##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937855_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 732937 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84109548296784 33.0 31.0 34.0 30.0 34.0 2 31.969302955097096 33.0 31.0 34.0 30.0 34.0 3 32.00447787463315 34.0 31.0 34.0 30.0 34.0 4 35.58007850606533 37.0 35.0 37.0 33.0 37.0 5 35.55016461169241 37.0 35.0 37.0 33.0 37.0 6 35.63795933347614 37.0 35.0 37.0 33.0 37.0 7 35.582728119879334 37.0 35.0 37.0 33.0 37.0 8 35.57416394587802 37.0 35.0 37.0 33.0 37.0 9 37.229744166278955 39.0 37.0 39.0 34.0 39.0 10-11 37.10533579284441 39.0 37.0 39.0 33.5 39.0 12-13 37.003226743908414 39.0 37.0 39.0 33.0 39.0 14-15 38.16540234699572 40.0 38.0 41.0 33.0 41.0 16-17 38.08723532854802 40.0 38.0 41.0 33.0 41.0 18-19 38.01664194876231 40.0 38.0 41.0 33.0 41.0 20-21 37.9003502347405 40.0 38.0 41.0 33.0 41.0 22-23 37.823867535681785 40.0 38.0 41.0 33.0 41.0 24-25 37.83295221826705 40.0 38.0 41.0 33.0 41.0 26-27 37.74895181986992 40.0 37.5 41.0 33.0 41.0 28-29 37.64076039277592 40.0 37.0 41.0 33.0 41.0 30-31 37.4461788666693 40.0 37.0 41.0 32.0 41.0 32-33 37.320022730466604 39.5 37.0 41.0 32.0 41.0 34-35 37.31885687310096 40.0 37.0 41.0 32.0 41.0 36-37 37.185250574060255 40.0 36.5 41.0 31.5 41.0 38-39 37.03107634080419 40.0 36.0 41.0 31.0 41.0 40-41 36.85547052475179 39.5 36.0 41.0 31.0 41.0 42-43 36.58596509659084 39.0 36.0 41.0 30.0 41.0 44-45 36.37020235027022 39.0 35.5 41.0 30.0 41.0 46-47 36.041417611609184 39.0 35.0 40.5 29.5 41.0 48-49 35.72740290093146 39.0 35.0 40.0 28.0 41.0 50-51 34.79653776518309 38.0 34.0 39.5 26.5 40.5 52-53 34.88916237002635 38.0 34.5 39.5 27.0 40.5 54-55 35.197371670416416 38.0 35.0 40.0 27.0 41.0 56-57 34.88950892095774 38.0 34.0 40.0 26.0 41.0 58-59 34.628255907397225 38.0 34.0 40.0 26.0 41.0 60-61 34.22715253834913 37.5 34.0 40.0 25.0 41.0 62-63 33.86123500382707 37.0 33.0 40.0 24.0 41.0 64-65 33.446078585199004 36.5 33.0 39.0 23.0 41.0 66-67 32.914543132629404 36.0 32.0 39.0 22.0 40.0 68-69 32.38490347737937 35.0 32.0 38.5 20.5 40.0 70-71 31.853979264247812 35.0 31.0 37.5 18.5 39.5 72-73 31.380965212562607 35.0 31.0 37.0 17.5 39.0 74-75 30.847700416270428 34.5 30.0 36.5 13.5 39.0 76-77 30.38233981911133 34.0 30.0 36.0 9.0 38.5 78-79 29.818041659787948 34.0 29.5 35.5 7.0 37.0 80-81 29.251488190663046 34.0 29.0 35.0 2.0 37.0 82-83 28.78394186676345 34.0 28.5 35.0 2.0 36.0 84-85 28.27514370266476 33.5 27.5 35.0 2.0 36.0 86-87 27.851344658545003 33.0 26.5 35.0 2.0 35.0 88-89 27.38889631714595 33.0 26.0 35.0 2.0 35.0 90-91 26.821743205759837 32.5 25.0 35.0 2.0 35.0 92-93 26.189332780307176 32.0 24.5 34.0 2.0 35.0 94-95 25.44912591395986 32.0 21.5 34.0 2.0 35.0 96-97 24.61637289425967 31.0 18.0 34.0 2.0 35.0 98-99 23.573068353760284 31.0 2.0 34.0 2.0 35.0 100-101 20.722716959302097 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2719612731154122 1101 2 0.36671518979772344 1101 3 0.37560596728499007 1101 4 0.16351777369605713 1101 5 0.1506794944202241 1101 6 0.25421674237227876 1101 7 0.28485747150509866 1101 8 0.21923998997272776 1101 9 0.3779308529347176 1101 10-11 0.4023089698502602 1101 12-13 0.40360503367698186 1101 14-15 0.552843918207941 1101 16-17 0.638621140515319 1101 18-19 0.49019646095781155 1101 20-21 0.6583183255882261 1101 22-23 0.5630194972326308 1101 24-25 0.4881661927044192 1101 26-27 0.5086623495809519 1101 28-29 0.6173424166323471 1101 30-31 0.6860226861927856 1101 32-33 0.7229208715176938 1101 34-35 0.6153507900003561 1101 36-37 0.5328966077398647 1101 38-39 0.6947419493945972 1101 40-41 0.6780022106743999 1101 42-43 0.7328236073874024 1101 44-45 0.8303161957259277 1101 46-47 0.9177391004392419 1101 48-49 0.9099560969018867 1101 50-51 0.7513930360076984 1101 52-53 0.7684359990258685 1101 54-55 0.6452531726623363 1101 56-57 0.5664269488380071 1101 58-59 0.44031126087299555 1101 60-61 0.6860083697180599 1101 62-63 0.8953138214654075 1101 64-65 0.941142619782255 1101 66-67 1.0316318042509351 1101 68-69 1.0301331208624092 1101 70-71 1.2962570741690307 1101 72-73 1.212029950458792 1101 74-75 1.2255519478874746 1101 76-77 1.1026098563008127 1101 78-79 1.2431652325552882 1101 80-81 1.3634224213657156 1101 82-83 1.3327622454754966 1101 84-85 1.3756833303523628 1101 86-87 1.4867834293319433 1101 88-89 1.400840431898697 1101 90-91 1.240834336914066 1101 92-93 1.377830011912284 1101 94-95 1.3588711081478984 1101 96-97 1.6989219991757274 1101 98-99 1.8083355958890373 1101 100-101 1.9776423996238144 1102 1 0.2410803214716033 1102 2 0.29193973631866754 1102 3 0.23906345050635025 1102 4 0.08360810830563281 1102 5 0.1349792979957627 1102 6 0.15070518222545815 1102 7 0.2729946039832427 1102 8 0.2669907407544727 1102 9 0.4469494530578686 1102 10-11 0.21497215522885682 1102 12-13 0.40520893739803654 1102 14-15 0.5143760289624382 1102 16-17 0.48724702932738495 1102 18-19 0.4026809379218008 1102 20-21 0.44698315529062427 1102 22-23 0.5981058586329908 1102 24-25 0.6334970698720568 1102 26-27 0.48551109587003793 1102 28-29 0.4322390271924803 1102 30-31 0.3910970419652813 1102 32-33 0.5260122346896523 1102 34-35 0.4951715311844751 1102 36-37 0.5021773364711635 1102 38-39 0.753574685886548 1102 40-41 0.7868294431535787 1102 42-43 0.6329223356164562 1102 44-45 0.8105825899439694 1102 46-47 0.5749870252544298 1102 48-49 0.7020437345452066 1102 50-51 0.7081977157945474 1102 52-53 0.7668260752908438 1102 54-55 0.7024579253773595 1102 56-57 0.644994151113913 1102 58-59 0.741522659683433 1102 60-61 0.6895704979821957 1102 62-63 0.6333735537639882 1102 64-65 0.6342466077835027 1102 66-67 0.7694197759190331 1102 68-69 0.7348841158847179 1102 70-71 0.7418593679663523 1102 72-73 0.7788890855719899 1102 74-75 0.7435999497588384 1102 76-77 0.7219927532691344 1102 78-79 1.0125608575531402 1102 80-81 0.8636236618325945 1102 82-83 0.9504870585970586 1102 84-85 1.2037740915229271 1102 86-87 1.337418282804805 1102 88-89 1.3654255497361731 1102 90-91 1.290454732033382 1102 92-93 1.2767453773959296 1102 94-95 1.3370433975076104 1102 96-97 1.5542825636122366 1102 98-99 1.6118526600486298 1102 100-101 1.7250279935583883 1103 1 0.24955807569032729 1103 2 0.2560520954754537 1103 3 0.17450950514388097 1103 4 0.27817764937139344 1103 5 0.29705895874541 1103 6 0.0818405143788965 1103 7 0.36025869706681135 1103 8 0.24185186890208854 1103 9 0.19170736919153342 1103 10-11 0.28754386917911745 1103 12-13 0.23050814709891654 1103 14-15 0.22926379246848683 1103 16-17 0.3512454872803161 1103 18-19 0.3272082479258316 1103 20-21 0.22645991068340976 1103 22-23 0.16536539566840247 1103 24-25 0.23562001307916347 1103 26-27 0.3448606323797563 1103 28-29 0.08993086310465515 1103 30-31 0.25625904452368076 1103 32-33 0.3989799743640745 1103 34-35 0.19738384302844736 1103 36-37 0.3443891226172653 1103 38-39 0.46503489931988184 1103 40-41 0.5696487607977474 1103 42-43 0.5241642092036471 1103 44-45 0.20512409379032448 1103 46-47 0.3339755825642641 1103 48-49 0.3301195051306536 1103 50-51 0.14760139918425352 1103 52-53 0.16570633884070674 1103 54-55 0.33768581540137177 1103 56-57 0.19815184294983368 1103 58-59 0.3591624515070819 1103 60-61 0.49344839589166867 1103 62-63 0.5178540766581321 1103 64-65 0.4910281102280081 1103 66-67 0.5674825792905125 1103 68-69 0.608416980762847 1103 70-71 0.41147490759520977 1103 72-73 0.2530659303334062 1103 74-75 0.0904518957171021 1103 76-77 0.07456341582367543 1103 78-79 0.1489181218214526 1103 80-81 0.2542837631170478 1103 82-83 0.6410113546000069 1103 84-85 0.6104013892606766 1103 86-87 0.6993034353447953 1103 88-89 0.9132640553523785 1103 90-91 0.7629572290982338 1103 92-93 0.8179127771190622 1103 94-95 1.056777219155851 1103 96-97 1.282932221032464 1103 98-99 1.2593582236843552 1103 100-101 1.1340253124992863 1104 1 0.24812222992679622 1104 2 0.3067025444021638 1104 3 0.35524794010798644 1104 4 0.3038357453463476 1104 5 0.34683854382556945 1104 6 0.39588049494639677 1104 7 0.30671316564194484 1104 8 0.3842147168427843 1104 9 0.25550894178451244 1104 10-11 0.42509717360361066 1104 12-13 0.4540700522035479 1104 14-15 0.35676257793730315 1104 16-17 0.24612466391813115 1104 18-19 0.42661537677410166 1104 20-21 0.34492798200787433 1104 22-23 0.34502064874071436 1104 24-25 0.3749519309588649 1104 26-27 0.48817981117674236 1104 28-29 0.3335928095933056 1104 30-31 0.3411106917878328 1104 32-33 0.3477420522020225 1104 34-35 0.28918170905215845 1104 36-37 0.18215024682332626 1104 38-39 0.15608695322706723 1104 40-41 0.1790168447173528 1104 42-43 0.11654954846268595 1104 44-45 0.2922095787931127 1104 46-47 0.19744518329837035 1104 48-49 -0.009731512376589535 1104 50-51 0.28646369833678165 1104 52-53 0.13956422969484095 1104 54-55 0.2994766641337634 1104 56-57 0.39783786414565014 1104 58-59 0.5086829365998398 1104 60-61 0.2847307608987748 1104 62-63 0.48922442770262364 1104 64-65 0.4757214186109664 1104 66-67 0.645976380683166 1104 68-69 0.6372931345674786 1104 70-71 0.4346508616769391 1104 72-73 0.5083181029947568 1104 74-75 0.48470131544108597 1104 76-77 0.919860930982825 1104 78-79 0.7830653409948525 1104 80-81 0.8602619555347744 1104 82-83 0.8516623433183597 1104 84-85 0.9611624954736762 1104 86-87 1.1705019527170926 1104 88-89 1.195777505160752 1104 90-91 1.474055345225409 1104 92-93 1.2831537940865125 1104 94-95 1.2049761143822941 1104 96-97 1.0780525046030078 1104 98-99 1.3649378699408992 1104 100-101 1.2697784118029531 1105 1 0.25886096410965465 1105 2 0.44628005999155107 1105 3 0.35628351402416314 1105 4 0.35947785695035606 1105 5 0.3188984992791575 1105 6 0.2655134518538773 1105 7 0.38477907796954725 1105 8 0.4445364326604775 1105 9 0.5879579160643544 1105 10-11 0.43628955237453226 1105 12-13 0.4272455271359661 1105 14-15 0.48690837633318296 1105 16-17 0.5878380274696511 1105 18-19 0.6042051482041515 1105 20-21 0.7104372352419546 1105 22-23 0.6306252417062481 1105 24-25 0.6855525266462692 1105 26-27 0.634060941421076 1105 28-29 0.7313684637525242 1105 30-31 0.8628001088545432 1105 32-33 0.7053097363464005 1105 34-35 0.7999511200206939 1105 36-37 0.8523292505156945 1105 38-39 0.8456230086758225 1105 40-41 0.9703059390270852 1105 42-43 0.9755274351482228 1105 44-45 0.8907294703646116 1105 46-47 0.9503975915190068 1105 48-49 1.0502202228139623 1105 50-51 1.0772954296601966 1105 52-53 1.0277705859248272 1105 54-55 1.1519668754503414 1105 56-57 1.2325783013549128 1105 58-59 1.3778659673175255 1105 60-61 1.1416876981285 1105 62-63 1.0706768712480468 1105 64-65 0.8454677866654592 1105 66-67 1.067686434860235 1105 68-69 1.2200693278944357 1105 70-71 0.7860348495103402 1105 72-73 0.9464988022767358 1105 74-75 0.858632740740326 1105 76-77 1.152640099028396 1105 78-79 1.0029100606834014 1105 80-81 0.9957074010546627 1105 82-83 1.1860941534718172 1105 84-85 1.245593004187743 1105 86-87 1.4380853285021402 1105 88-89 1.6051908162828497 1105 90-91 1.4515832053243933 1105 92-93 1.7101802281797625 1105 94-95 2.0105178292332013 1105 96-97 1.993287897805029 1105 98-99 2.098057271427514 1105 100-101 2.181149459610399 1106 1 0.25731936593863836 1106 2 0.2745631526087706 1106 3 0.2678176658953717 1106 4 0.17554949368511785 1106 5 0.36230352802948573 1106 6 0.3505202201359694 1106 7 0.09968333473128865 1106 8 0.21812133832085578 1106 9 0.4549189455093554 1106 10-11 0.37486471599091686 1106 12-13 0.4039157702469467 1106 14-15 0.4848671861622975 1106 16-17 0.44826391775448826 1106 18-19 0.42918022807939593 1106 20-21 0.22370972937174827 1106 22-23 0.20790974507612248 1106 24-25 0.17036968786936058 1106 26-27 0.4136266770227195 1106 28-29 0.4560825662830794 1106 30-31 0.3051590871175307 1106 32-33 0.40104750288467983 1106 34-35 0.2652128229632922 1106 36-37 0.1915462709014264 1106 38-39 0.2360077658860078 1106 40-41 0.4747059171402057 1106 42-43 0.586851996280437 1106 44-45 0.6443092849446117 1106 46-47 0.6143732019133807 1106 48-49 0.36644054764392564 1106 50-51 0.3194940626813221 1106 52-53 0.5444819383203736 1106 54-55 0.47173787199363915 1106 56-57 0.36889792604237215 1106 58-59 0.22391431354905222 1106 60-61 0.1374340291010654 1106 62-63 0.5898100553381838 1106 64-65 0.6202636081731399 1106 66-67 0.6375805451463634 1106 68-69 0.5248133107846016 1106 70-71 0.5163455719441608 1106 72-73 0.4696429964229445 1106 74-75 0.5933148931729804 1106 76-77 0.7033879406720054 1106 78-79 0.5760883936162671 1106 80-81 0.6450616189195948 1106 82-83 0.5645220088185212 1106 84-85 0.38281784199876867 1106 86-87 0.510996022863651 1106 88-89 0.6468567890717409 1106 90-91 0.8519288754081131 1106 92-93 0.9992037161407836 1106 94-95 0.9570638642569804 1106 96-97 0.8183069893564543 1106 98-99 1.052649116658671 1106 100-101 1.1236761580447734 1107 1 0.30379027042136997 1107 2 0.14874968137147704 1107 3 0.25911333164894046 1107 4 0.16986351534355038 1107 5 0.27889808526048654 1107 6 0.07656154012266114 1107 7 0.040665388442150174 1107 8 0.19379016400375804 1107 9 0.11305365788231114 1107 10-11 0.12096568626817117 1107 12-13 0.07037592231678502 1107 14-15 0.15828312533436417 1107 16-17 0.24006648297299193 1107 18-19 0.21029950305955936 1107 20-21 0.11632768590128251 1107 22-23 0.029228963734112767 1107 24-25 0.14140985581180843 1107 26-27 0.2363411492584504 1107 28-29 0.24532503319380083 1107 30-31 0.27797596328557006 1107 32-33 0.2695198922244302 1107 34-35 0.3718754788092724 1107 36-37 0.35672509376822603 1107 38-39 0.06673278572677077 1107 40-41 0.04853787228728379 1107 42-43 0.08019446063106983 1107 44-45 0.25373172676638234 1107 46-47 0.2934917600660114 1107 48-49 0.20499178801155438 1107 50-51 0.3269362684620276 1107 52-53 0.21593172199206379 1107 54-55 0.25530076080856645 1107 56-57 0.41243111771692753 1107 58-59 0.025324927716638967 1107 60-61 0.21621209417876486 1107 62-63 0.38177774689753363 1107 64-65 0.3505943728745038 1107 66-67 0.4163957316495157 1107 68-69 0.27465458468952164 1107 70-71 0.30446799584523987 1107 72-73 0.3905897207771609 1107 74-75 0.31693692025882214 1107 76-77 0.11427091857835947 1107 78-79 0.2996092968189501 1107 80-81 0.4132690380844899 1107 82-83 0.5567981922239653 1107 84-85 0.31699259430925864 1107 86-87 0.5768302308972366 1107 88-89 0.6802379109068184 1107 90-91 0.8697638002692258 1107 92-93 0.6698009870759876 1107 94-95 0.841511267037248 1107 96-97 1.4008727198805069 1107 98-99 1.0381922415146647 1107 100-101 1.345389631842167 1108 1 0.22210645648136662 1108 2 0.09306693007938804 1108 3 0.22382859030710733 1108 4 0.22170624345058343 1108 5 0.22389156446639902 1108 6 0.2974135905501356 1108 7 0.3845479175442179 1108 8 0.3053778566087004 1108 9 0.46891029701673403 1108 10-11 0.3286843743810053 1108 12-13 0.3798576399422515 1108 14-15 0.3215130920058584 1108 16-17 0.4327293820697449 1108 18-19 0.44851860174090774 1108 20-21 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-0.6626013619072353 2316 54-55 -0.73718571688471 2316 56-57 -0.6868937836617306 2316 58-59 -0.7756673859195402 2316 60-61 -0.9272097611223913 2316 62-63 -0.9611174501417921 2316 64-65 -0.972722964146115 2316 66-67 -1.2337710095762304 2316 68-69 -1.1823973491020148 2316 70-71 -1.0526693419887536 2316 72-73 -0.936442692090381 2316 74-75 -1.0801349411426173 2316 76-77 -0.8702750165959046 2316 78-79 -0.726166456566709 2316 80-81 -0.7309438213241854 2316 82-83 -1.0961848489733192 2316 84-85 -1.1741294521342738 2316 86-87 -1.202133940073523 2316 88-89 -1.1573154666197176 2316 90-91 -1.066431246296311 2316 92-93 -1.1879016441818884 2316 94-95 -1.36436737788528 2316 96-97 -1.5600610309837002 2316 98-99 -1.7513864318758756 2316 100-101 -1.6684132881627143 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2671.0 3 489.0 4 399.0 5 476.0 6 604.0 7 653.0 8 771.0 9 1025.0 10 1309.0 11 1708.0 12 2227.0 13 2825.0 14 3253.0 15 4051.0 16 5345.0 17 6646.0 18 5834.0 19 4646.0 20 4756.0 21 5266.0 22 5827.0 23 6251.0 24 7157.0 25 8320.0 26 9576.0 27 11451.0 28 13785.0 29 16711.0 30 20272.0 31 25390.0 32 32550.0 33 42346.0 34 56724.0 35 80014.0 36 113275.0 37 141137.0 38 82127.0 39 5070.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25839547131798 18.03466040359579 14.767078365031919 22.93986576005431 2 15.645154670205635 20.93103118030948 37.61817460426044 25.805639545224444 3 20.933411042340463 22.508386553058287 30.66878998319953 25.88941242140172 4 13.492266542621234 15.706887941826341 36.36948208752044 34.431363428031986 5 13.817501648801233 36.77428800521049 34.421086046899845 14.987124299088439 6 33.118083198460255 34.07673002055903 16.340738594112246 16.464448186868466 7 27.819720512389395 32.40968186857275 20.89882756273298 18.871770056304875 8 25.673994646865356 31.63516664980712 20.549830903279958 22.14100780004757 9 24.71748690126155 17.625919440537263 21.8583873617849 35.79820629641628 10-11 25.524247761963252 24.985287091396383 26.92909984340407 22.561365303236293 12-13 26.852301168867204 22.89864304236272 27.18829410454055 23.06076168422952 14-15 23.40338718937095 24.78908663440772 26.87470953279204 24.93281664342929 16-17 22.27175328336939 27.720830727484614 27.193987922531964 22.813428066614037 18-19 21.79749785564657 27.495224328415844 28.609331269286365 22.09794654665122 20-21 23.01296635090815 25.483906020096374 28.058314066847135 23.444813562148344 22-23 22.55180548664559 25.13486990026393 28.849789032996142 23.463535580094337 24-25 24.12330100272277 25.367819925424545 27.912010256858867 22.596868814993822 26-27 23.004340299727392 24.842970812492354 29.617778895523223 22.53490999225703 28-29 22.02467812698853 27.208137370760387 28.355172010769085 22.412012491481992 30-31 22.290673981351926 25.541407238448848 28.19288950801542 23.975029272183804 32-33 22.647046157821237 25.773859691214863 28.558353309969725 23.02074084099418 34-35 24.193563821788405 25.27252594020424 27.517395999945315 23.01651423806204 36-37 23.028850983396282 24.77587339161525 29.223007256652046 22.972268368336422 38-39 22.637948482469323 25.958360409521795 28.648213176554517 22.755477931454365 40-41 22.762729272457786 26.226015270138493 28.25590636465331 22.75534909275041 42-43 22.451187499188425 26.154301425436422 26.847097562125775 24.547413513249374 44-45 23.14470111342196 25.326846858176243 27.329106371403554 24.199345656998236 46-47 23.864986227966455 26.336912467278605 27.23677645563218 22.56132484912276 48-49 23.49647673139092 25.901731220055634 27.27337967234627 23.328412376207172 50-51 21.783206148609626 26.60524436212276 28.561166568131256 23.050382921136357 52-53 23.32294624923259 26.932195904325795 26.153506427093742 23.59135141934787 54-55 22.401405644858716 25.63771020944573 27.31569534246875 24.645188803226805 56-57 23.632995475719145 25.9596415834778 26.7460778974428 23.66128504336025 58-59 24.811731577505228 25.352638149492744 26.69889146951524 23.136738803486782 60-61 23.546352532186553 25.52335818340955 28.37281536946809 22.557473914935805 62-63 22.426221129227223 26.28659048652475 28.14000509750516 23.147183286742866 64-65 22.451761551501832 27.150393813234064 27.11684435579917 23.28100027946493 66-67 22.971581471209422 25.95487445653177 26.441920387668883 24.63162368458993 68-69 22.29323737290562 25.713672652576463 27.67249267278993 24.320597301727982 70-71 23.855038377781135 25.742597936284618 26.719449639454535 23.68291404647972 72-73 23.22911527849712 24.88824994841254 28.539753774083827 23.342880999006514 74-75 22.347693346325357 27.454146400794826 26.670525097337684 23.527635155542132 76-77 22.96662716976697 25.779653766623888 26.821469913339158 24.432249150269985 78-79 23.107148812175915 25.88502529815191 27.344965107411223 23.662860782260953 80-81 23.673871005213638 25.761853676535907 26.727777132431825 23.836498185818638 82-83 23.073622268640275 25.441899650907988 27.93378389366394 23.550694186787798 84-85 22.87579555819745 26.279811013292615 27.19010381970422 23.654289608805716 86-87 22.828536935146992 26.49865095963264 27.093677171804682 23.579134933415684 88-89 22.71682797590781 25.95854585953968 26.039445319412795 25.28518084513971 90-91 22.90819066778002 25.049916430124515 26.98384497958927 25.058047922506194 92-93 23.726071238477605 25.640235507456804 27.32530133653283 23.308391917532763 94-95 23.000205669508915 25.342104921514192 27.28557928764555 24.372110121331346 96-97 21.6508461883261 25.511853742798106 28.391859537110935 24.445440531764863 98-99 22.40563700954785 25.94441570758833 26.752016268173406 24.897931014690418 100-101 22.213713027129835 24.42457042607612 27.956873182782065 25.40484336401198 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1035.0 1 620.0 2 201.0 3 206.5 4 185.0 5 179.0 6 261.5 7 464.0 8 735.5 9 862.5 10 795.5 11 727.0 12 763.0 13 834.5 14 895.0 15 927.0 16 931.0 17 928.0 18 951.5 19 1020.0 20 1088.5 21 1144.0 22 1288.5 23 1489.0 24 1643.0 25 1947.0 26 2338.0 27 2828.0 28 3339.0 29 3835.5 30 4601.5 31 5725.5 32 6917.0 33 7634.5 34 8483.0 35 9697.0 36 11119.5 37 12439.5 38 14082.5 39 15280.5 40 15580.5 41 17621.5 42 20228.0 43 22329.5 44 24182.0 45 25397.5 46 27474.0 47 36606.0 48 52814.0 49 52758.0 50 38627.0 51 31037.5 52 28426.0 53 28911.0 54 29173.0 55 26028.5 56 22142.0 57 18803.0 58 16265.0 59 14378.0 60 12136.0 61 9872.0 62 7802.5 63 5893.5 64 4435.0 65 3481.0 66 2608.0 67 1983.5 68 1507.5 69 1163.0 70 860.0 71 607.5 72 475.0 73 364.0 74 290.5 75 254.0 76 188.5 77 115.5 78 66.0 79 38.0 80 24.5 81 16.5 82 12.5 83 6.5 84 5.0 85 7.5 86 3.5 87 2.0 88 2.5 89 1.5 90 1.5 91 2.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3144881483674586 2 0.2765585582389755 3 0.273556935998592 4 0.27587637136616106 5 0.2869277987057551 6 0.18896576376960095 7 0.18882932639503805 8 0.1914216365117329 9 0.18801070214766072 10-11 0.19599228855958972 12-13 0.18746495264940916 14-15 0.18514551728184006 16-17 0.1923084794463917 18-19 0.18616879759106172 20-21 0.1790740541137915 22-23 0.17818721117913272 24-25 0.18132527079407917 26-27 0.17811899249185129 28-29 0.19080766832619994 30-31 0.19653803805784126 32-33 0.19885747342541035 34-35 0.19742488099250005 36-37 0.17238862276020994 38-39 0.1644752550355624 40-41 0.17020562476720374 42-43 0.18234855110330084 44-45 0.18603236021649883 46-47 0.18855645164591225 48-49 0.1871920779002834 50-51 0.18957973195513392 52-53 0.21557105180936426 54-55 0.17948336623748018 56-57 0.16624894090487993 58-59 0.16406594291187374 60-61 0.15669832468547773 62-63 0.1646116924101253 64-65 0.16120075804605308 66-67 0.1607914459223644 68-69 0.17006918739264085 70-71 0.1635201934136222 72-73 0.15963172823857985 74-75 0.16454347372284386 76-77 0.16836372021060475 78-79 0.1723204040729285 80-81 0.1639977242245923 82-83 0.16120075804605308 84-85 0.15547038831441173 86-87 0.15287807819771684 88-89 0.15888132267848398 90-91 0.16563497271934696 92-93 0.15765338630741796 94-95 0.16106432067149018 96-97 0.16167828885702318 98-99 0.16392950553731084 100-101 0.1431228059164703 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 732937.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.32947946866873 #Duplication Level Percentage of deduplicated Percentage of total 1 66.65773791771124 24.88298659027103 2 15.050460686292919 11.236517263659547 3 6.583990900174721 7.373308593899221 4 3.494241258522007 5.217528292742899 5 1.9983652084521388 3.729896650990801 6 1.317775394569393 2.9515121721537 7 0.8906617614552704 2.327355795644099 8 0.6419522199336299 1.9170993771103002 9 0.46271428068842685 1.5545594916736605 >10 2.5654144510150165 17.79562484687206 >50 0.22653786028854075 5.867221653643323 >100 0.09876609460409687 6.2578394837645295 >500 0.003304441826902823 0.8577151756405909 >1k 0.007343204059784052 5.882846080032829 >5k 7.343204059784052E-4 2.147988531901461 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8841 1.2062428285104996 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6831 0.9320037056390932 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4879 0.6656779504923342 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3171 0.4326429147389203 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2710 0.36974528506542853 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2604 0.35528292336176237 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2540 0.34655093138973747 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2512 0.34273068490197656 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2455 0.3349537545518919 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2406 0.32826832319831034 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2395 0.32676751207811855 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2267 0.3093035281340688 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2127 0.2902022956952644 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1757 0.23972046710699557 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1730 0.23603665799379758 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1579 0.21543461443480136 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1520 0.2073848093355909 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1520 0.2073848093355909 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1245 0.1698645313307965 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1237 0.1687730323342934 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1208 0.16481634847196963 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1060 0.14462361703666207 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 947 0.12920619371105566 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 840 0.11460739463282656 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 807 0.11010496127225122 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 774 0.10560252791167589 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.3643737456288876E-4 0.0 18-19 0.0 0.0 0.0 2.728747491257775E-4 0.0 20-21 0.0 0.0 0.0 2.728747491257775E-4 0.0 22-23 0.0 0.0 0.0 4.093121236886663E-4 0.0 24-25 0.0 0.0 0.0 5.45749498251555E-4 0.0 26-27 0.0 0.0 0.0 9.550616219402213E-4 0.0 28-29 0.0 0.0 0.0 0.004297777298730996 0.0 30-31 0.0 0.0 0.0 0.010710333903186767 0.0 32-33 0.0 0.0 0.0 0.01944232587521165 0.0 34-35 0.0 0.0 0.0 0.029061160781895308 0.0 36-37 0.0 0.0 0.0 0.0358148108227583 0.0 38-39 0.0 0.0 0.0 0.04147696186711819 0.0 40-41 0.0 0.0 0.0 0.04659336341322651 0.0 42-43 0.0 0.0 0.0 0.052937701330400835 0.0 44-45 0.0 0.0 0.0 0.06098750642961128 0.0 46-47 0.0 0.0 0.0 0.07142496558367227 0.0 48-49 0.0 0.0 0.0 0.08629663941102714 0.0 50-51 0.0 0.0 0.0 0.10389706072963979 0.0 52-53 0.0 0.0 0.0 0.1360280624392001 0.0 54-55 0.0 0.0 0.0 0.17859652330282139 0.0 56-57 0.0 0.0 0.0 0.23931115498330688 0.0 58-59 0.0 0.0 0.0 0.31257802512357813 0.0 60-61 6.821868728144438E-5 0.0 0.0 0.41777124091156537 0.0 62-63 1.3643737456288876E-4 0.0 0.0 0.4958816378488192 0.0 64-65 1.3643737456288876E-4 0.0 0.0 0.5755610645935463 0.0 66-67 1.3643737456288876E-4 0.0 0.0 0.7186156518227351 0.0 68-69 1.3643737456288876E-4 0.0 0.0 0.8925733043904183 0.0 70-71 1.3643737456288876E-4 0.0 0.0 1.0511817523197764 0.0 72-73 1.3643737456288876E-4 0.0 0.0 1.208903357314476 0.0 74-75 1.3643737456288876E-4 0.0 0.0 1.4849161660552 0.0 76-77 1.3643737456288876E-4 0.0 0.0 1.7022609037338816 0.0 78-79 1.3643737456288876E-4 0.0 0.0 1.9263592914534264 0.0 80-81 1.3643737456288876E-4 0.0 0.0 2.135108474534646 0.0 82-83 1.3643737456288876E-4 0.0 0.0 2.4058684443546987 0.0 84-85 1.3643737456288876E-4 0.0 0.0 2.703643014338204 0.0 86-87 1.3643737456288876E-4 0.0 0.0 3.112818700652307 0.0 88-89 1.3643737456288876E-4 0.0 0.0 3.63721574978477 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCTCGT 55 0.0025789961 34.545506 6 GTACATG 2005 0.0 25.148367 1 TACTATA 115 1.2728122E-4 24.806393 2 TACATGG 2040 0.0 23.539724 2 ACATGGG 2105 0.0 21.909367 3 ACTATAC 110 0.002860408 21.61163 3 TATAAGC 420 0.0 21.508717 3 GTTCTAG 155 3.8608465E-5 21.482492 1 GAGTACT 2160 0.0 21.218195 12-13 CATGGGG 1440 0.0 20.80404 4 ATATAGC 115 0.0037013704 20.671995 3 GTACTTT 2270 0.0 20.399223 14-15 TATAACT 210 8.5088504E-7 20.379469 4 GTTCCGG 120 0.0047224048 19.820158 1 CCCTAAT 145 6.083871E-4 19.683466 1 AGTACTT 2180 0.0 19.28065 12-13 TCTAAAG 450 0.0 19.018234 3 ATCGGAC 200 1.2844308E-5 18.99873 8 CCTATAA 380 1.0913936E-11 18.77699 1 GGATCGG 205 1.588086E-5 18.536612 6 >>END_MODULE