FastQCFastQC Report
Thu 26 May 2016
SRR937855_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937855_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732937
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT110331.5053135535523516No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT57450.7838327168637959No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46060.6284305472366657No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33500.45706520478567736No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28750.3922574518683052No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28180.38448052151822054No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA26010.35487361123807365No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25990.35460073648894785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25680.35037117787749833No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24610.33577237879926924No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24030.3278590110746217No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23930.3264946373289928No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20730.2828346774688684No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA17690.24135771560175023No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC16360.22321154478488603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14520.19810706786531446No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA14140.1929224476319247No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT13790.18814713952222362No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC13440.1833718314125225No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG12670.17286615357118007No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC10640.14516936653491364No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG10560.14407786753841054No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT10260.13998474630152385No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC8110.11065071077050279No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG7530.10273734304585523No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAT753.063741E-431.6906341
TATTTCG600.00394200531.679825
CTAGAAC1657.6397555E-1131.6798173
AACGTAC300.00394987331.6690138-39
ATATATC700.00838938527.1430158
GTAGTGC700.008392177527.1411636
GTCTAGA1351.33402045E-524.648271
CCCTAAT1201.701242E-423.7679731
TAAGCCG1302.9318564E-421.9321825
ATCGGAC2405.9189915E-921.770968
GGCGCGC1100.002876286221.5910328
GAGTACT26100.021.29322612-13
GGATCGG2509.482392E-920.8986936
GTGTAAG1604.9571456E-520.7969781
ATAAGGC3800.020.0083053
CCTATAA4050.019.953361
AGTACTT26500.019.89634112-13
GACGCAT1200.00476172419.791787
GTACTTT28050.019.72693314-15
TCGGACC2651.8519131E-819.7170949
Produced by FastQC (version 0.11.5)