FastQCFastQC Report
Thu 26 May 2016
SRR937854_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937854_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733484
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86301.1765764488386932No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT67950.9264005758816825No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46880.6391414127642866No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC31440.4286392068538646No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC26460.3607440653102181No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT26090.35569964716340097No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC26000.3544726265330941No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24740.337294337708798No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24050.32788717954311203No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23670.3227064257707053No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA22850.31152690447235387No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG22200.3026650888090265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20820.28385077247765456No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA17980.24513145481019355No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC17220.23476994726538003No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT15860.2162283021851874No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG15270.20818450027539795No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA14420.1965959721002776No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC13230.18037203265510904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12370.168647168854399No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC11780.16060336694460955No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC10890.14846949626713057No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10120.1379716530967274No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG8500.11588528175120384No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG8080.11015918547643848No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT7550.10293339732018694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG804.4618815E-429.7216915
ATACCGT700.00837050527.155556
TAGACTG1551.3954086E-624.5443655
GAGTACT23000.024.16525812-13
TAAGGTG2002.0265361E-823.7773515
GTACTTT24850.022.07798214-15
GTGCTAG2858.185452E-1121.699041
CTAGTCA1100.002856095621.6172524
TCAATAC2005.328329E-721.396693
TACATGG20250.021.3688012
ATAAGGC2457.4196578E-921.3481713
GTACATG21250.021.267021
GGTAAGG2056.756072E-720.874823
ACATGGG20300.020.8462583
GTATATA2351.11278496E-720.2429681
AGTACTT23550.019.96998212-13
GTAAGCG1452.4519977E-919.6475894-95
ACTTTTT29350.019.09559416-17
CTATTAG1250.00597732419.0283871
CATGGGC4750.019.0231824
Produced by FastQC (version 0.11.5)