##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937854_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 733484 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.802396507626614 33.0 31.0 34.0 30.0 34.0 2 31.919529260351965 33.0 31.0 34.0 30.0 34.0 3 31.971422689520153 34.0 31.0 34.0 30.0 34.0 4 35.543669664232624 37.0 35.0 37.0 33.0 37.0 5 35.513527220771 37.0 35.0 37.0 33.0 37.0 6 35.61356212269116 37.0 35.0 37.0 33.0 37.0 7 35.555724460247255 37.0 35.0 37.0 33.0 37.0 8 35.55682741545828 37.0 35.0 37.0 33.0 37.0 9 37.178491964378225 39.0 37.0 39.0 34.0 39.0 10-11 37.07528521412873 39.0 37.0 39.0 33.0 39.0 12-13 36.966479159736274 39.0 37.0 39.0 33.0 39.0 14-15 38.11041958652132 40.0 38.0 41.0 33.0 41.0 16-17 38.06260804598328 40.0 38.0 41.0 33.0 41.0 18-19 38.0034929187276 40.0 38.0 41.0 33.0 41.0 20-21 37.82286457509639 40.0 38.0 41.0 33.0 41.0 22-23 37.685688440374975 40.0 37.0 41.0 32.5 41.0 24-25 37.50850120793365 40.0 37.0 41.0 32.0 41.0 26-27 37.607534043005714 40.0 37.0 41.0 32.0 41.0 28-29 37.57283253622438 40.0 37.0 41.0 32.0 41.0 30-31 37.41510721433596 40.0 37.0 41.0 32.0 41.0 32-33 37.18856171368428 39.0 36.5 41.0 31.5 41.0 34-35 36.90271566932612 39.0 36.0 41.0 31.0 41.0 36-37 36.94702065757399 39.0 36.0 41.0 31.0 41.0 38-39 36.90974445250339 39.0 36.0 41.0 31.0 41.0 40-41 36.78038239416266 39.0 36.0 41.0 30.5 41.0 42-43 36.54094636011146 39.0 36.0 41.0 30.0 41.0 44-45 36.339123961804205 39.0 35.5 41.0 30.0 41.0 46-47 35.99232362260118 39.0 35.0 40.5 29.0 41.0 48-49 35.6785376368128 39.0 35.0 40.0 28.0 41.0 50-51 34.7331727754116 38.0 34.0 39.5 26.5 40.5 52-53 34.85292112711389 38.0 34.5 39.5 27.0 40.5 54-55 35.17575570837265 38.0 35.0 40.0 27.0 41.0 56-57 34.83314632630024 38.0 34.0 40.0 26.0 41.0 58-59 34.58781922986732 38.0 34.0 40.0 26.0 41.0 60-61 34.20379858320018 37.5 34.0 40.0 25.0 41.0 62-63 33.844197010432396 37.0 33.0 40.0 24.0 41.0 64-65 33.46248929765339 36.5 33.0 39.0 22.5 41.0 66-67 32.96726445293967 36.0 32.5 39.0 22.0 40.5 68-69 32.45698474677021 35.5 32.0 38.5 20.5 40.0 70-71 31.93518127184778 35.0 31.0 37.5 19.0 40.0 72-73 31.446880231879632 35.0 31.0 37.0 18.0 39.0 74-75 30.957384891831314 35.0 30.5 36.5 14.0 39.0 76-77 30.52162010350601 34.0 30.0 36.0 10.0 38.5 78-79 29.98826831941801 34.0 29.5 35.5 7.0 37.0 80-81 29.44749919561981 34.0 29.0 35.0 4.0 37.0 82-83 28.972496332571673 34.0 29.0 35.0 2.0 36.0 84-85 28.51663158296568 34.0 28.5 35.0 2.0 36.0 86-87 28.098187554193412 33.5 27.0 35.0 2.0 35.5 88-89 27.596878050509623 33.0 26.0 35.0 2.0 35.0 90-91 27.068696795022113 33.0 25.5 35.0 2.0 35.0 92-93 26.503409072317872 32.0 25.0 34.5 2.0 35.0 94-95 25.872216026525457 32.0 23.5 34.0 2.0 35.0 96-97 25.105413342349664 32.0 19.5 34.0 2.0 35.0 98-99 24.182620888799214 31.5 9.0 34.0 2.0 35.0 100-101 21.61905713008055 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34406912193778183 1101 2 0.2543116227045772 1101 3 0.4489944618681072 1101 4 0.2204408579415471 1101 5 0.14449092588925083 1101 6 0.34414619977046357 1101 7 0.26072509975915636 1101 8 0.18602520293066505 1101 9 0.3632239594737712 1101 10-11 0.1562176279217269 1101 12-13 0.3560260358227154 1101 14-15 0.5823060021568764 1101 16-17 0.7355388127415381 1101 18-19 0.5931552212431228 1101 20-21 0.5818106322911802 1101 22-23 0.577214566317565 1101 24-25 0.5860052537798808 1101 26-27 0.5481297058891172 1101 28-29 0.45882670969673 1101 30-31 0.5045338275885527 1101 32-33 0.47522457611888314 1101 34-35 0.6116208992306653 1101 36-37 0.7615646565435199 1101 38-39 0.6162028303628446 1101 40-41 0.9677666062050037 1101 42-43 0.8804967198239524 1101 44-45 1.0519272536976345 1101 46-47 1.1013480216601863 1101 48-49 1.1338934609954592 1101 50-51 1.1273689199967265 1101 52-53 0.9203815088405563 1101 54-55 1.0021734708422514 1101 56-57 1.3216342374836145 1101 58-59 1.3700065632091167 1101 60-61 1.2779446277109017 1101 62-63 1.1482822244990416 1101 64-65 1.3875949971233865 1101 66-67 1.5217283763708522 1101 68-69 1.5626830109466212 1101 70-71 1.4243637837073067 1101 72-73 1.5633450711593788 1101 74-75 1.4862831633452522 1101 76-77 1.396958788627277 1101 78-79 1.4799464854657778 1101 80-81 1.5269209175323866 1101 82-83 1.7101966769589332 1101 84-85 1.9489261176590773 1101 86-87 1.8608541382300636 1101 88-89 1.9142972517149168 1101 90-91 2.247374722963567 1101 92-93 2.1998120451948786 1101 94-95 2.4805695160520607 1101 96-97 2.4044593796084364 1101 98-99 2.547730011745074 1101 100-101 2.3975237618544334 1102 1 0.03885271366904419 1102 2 0.19049702322136852 1102 3 0.21382327323761885 1102 4 0.036742537528105856 1102 5 -0.025854681345897745 1102 6 0.09864878374979469 1102 7 0.1116359524723407 1102 8 0.14019186959733076 1102 9 0.10099204732907197 1102 10-11 0.08753868735325199 1102 12-13 0.18765717277362626 1102 14-15 0.20110283678219787 1102 16-17 0.291283321785464 1102 18-19 0.1757731152999682 1102 20-21 0.211647518596088 1102 22-23 0.175962951330483 1102 24-25 0.297071145252751 1102 26-27 0.2414371994291642 1102 28-29 0.1966577820481561 1102 30-31 0.15947568805366785 1102 32-33 0.1281913073850447 1102 34-35 0.2211202532358314 1102 36-37 0.276708390393658 1102 38-39 0.3716081921199432 1102 40-41 0.4584933503910449 1102 42-43 0.562355569953148 1102 44-45 0.22753125886558934 1102 46-47 0.26408057979972455 1102 48-49 0.44007401655101575 1102 50-51 0.3706085840794202 1102 52-53 0.5770126457914628 1102 54-55 0.6509234708422511 1102 56-57 0.7115874028582851 1102 58-59 0.4522917699274629 1102 60-61 0.5069126509667186 1102 62-63 0.44763138470576536 1102 64-65 0.38145481624482613 1102 66-67 0.3458724332183962 1102 68-69 0.4463264217993341 1102 70-71 0.661700670012209 1102 72-73 0.8071225259397359 1102 74-75 0.6647279307871123 1102 76-77 0.666663245991618 1102 78-79 0.6721880875329624 1102 80-81 0.7270921061628783 1102 82-83 1.0727968061578963 1102 84-85 1.1899580944032628 1102 86-87 1.1007410891344556 1102 88-89 1.299148672903545 1102 90-91 1.4899118676664038 1102 92-93 1.6020488023008248 1102 94-95 1.5263980044241556 1102 96-97 1.6102765630709648 1102 98-99 2.0670339523135475 1102 100-101 2.5604162037148974 1103 1 0.2288216926318647 1103 2 0.2646618797739819 1103 3 0.3094604001714494 1103 4 0.20106103789012764 1103 5 0.11064452486097309 1103 6 0.15557936172419318 1103 7 0.1546004210959211 1103 8 0.03779255511575741 1103 9 0.1254701034326402 1103 10-11 0.2662401214949952 1103 12-13 0.3709071412211742 1103 14-15 0.5743898710514728 1103 16-17 0.5647467176258587 1103 18-19 0.5285327919372094 1103 20-21 0.4802119819055761 1103 22-23 0.44549059459520635 1103 24-25 0.4499576959392684 1103 26-27 0.08172867761147273 1103 28-29 0.41199991021087357 1103 30-31 0.46015722090475464 1103 32-33 0.35359700542480255 1103 34-35 0.31582077069596437 1103 36-37 0.562527060142493 1103 38-39 0.7015322005427933 1103 40-41 0.6839073517062815 1103 42-43 0.8244700488727972 1103 44-45 0.7166091303043132 1103 46-47 1.0253647311717557 1103 48-49 1.3073976383733523 1103 50-51 1.052269305600845 1103 52-53 0.8688744394318135 1103 54-55 1.1232885094026628 1103 56-57 1.1866294174321936 1103 58-59 1.1323732084533304 1103 60-61 1.3507579310528044 1103 62-63 1.1476058106172928 1103 64-65 1.2155288017506365 1103 66-67 1.4300991989929557 1103 68-69 1.4453051189157762 1103 70-71 1.5838641050440643 1103 72-73 1.3626847241156703 1103 74-75 1.4698483947077214 1103 76-77 1.21515769608229 1103 78-79 1.051752398062174 1103 80-81 1.1295269586634937 1103 82-83 0.9372757258021167 1103 84-85 0.9547246395099798 1103 86-87 1.0589357577673404 1103 88-89 1.0828383828203165 1103 90-91 1.326765789801609 1103 92-93 1.4302988703876842 1103 94-95 1.3094882178515483 1103 96-97 1.4857158063436522 1103 98-99 1.6126255772977736 1103 100-101 1.7284379649392676 1104 1 0.08911516559398791 1104 2 0.215152345760508 1104 3 0.16756369788447856 1104 4 0.1460769425254469 1104 5 0.23265429560275663 1104 6 0.21291495829706975 1104 7 0.2062878555572496 1104 8 0.19385262448592044 1104 9 0.29717177666340433 1104 10-11 0.31376026093673204 1104 12-13 0.3110175092197167 1104 14-15 0.4890940308062639 1104 16-17 0.3850979873663647 1104 18-19 0.29537469600438016 1104 20-21 0.3659213075981427 1104 22-23 0.3791032088823698 1104 24-25 0.209494339728046 1104 26-27 0.38924839620289475 1104 28-29 0.4079527328890933 1104 30-31 0.32689058065813725 1104 32-33 0.3671098080424926 1104 34-35 0.35114852542439223 1104 36-37 0.4144833127508889 1104 38-39 0.5399519777025361 1104 40-41 0.5293474384014516 1104 42-43 0.4672233569317328 1104 44-45 0.5058034474220463 1104 46-47 0.4808466574037027 1104 48-49 0.4991160053337964 1104 50-51 0.39205684632688076 1104 52-53 0.5786130913780667 1104 54-55 0.7856437982638056 1104 56-57 0.9102947081520938 1104 58-59 0.920832791039949 1104 60-61 0.8343421379428264 1104 62-63 0.46663403213887733 1104 64-65 0.4505912454180674 1104 66-67 0.5845609138879055 1104 68-69 0.5633378540571314 1104 70-71 0.5049239815244917 1104 72-73 0.37113241768052063 1104 74-75 0.3744030473834492 1104 76-77 0.5722307872630203 1104 78-79 0.5105774540878762 1104 80-81 0.8507732367684042 1104 82-83 0.8304200739575656 1104 84-85 0.8574126456263365 1104 86-87 1.0855659390486174 1104 88-89 0.8710673744980006 1104 90-91 1.2494731199622002 1104 92-93 1.413433293489561 1104 94-95 1.3359097275118152 1104 96-97 1.6944303891582315 1104 98-99 2.3541138794121963 1104 100-101 1.9899666336143262 1105 1 0.08711489658566762 1105 2 0.23246303115527667 1105 3 0.1891881238399371 1105 4 0.11781761850492245 1105 5 0.1809874047624902 1105 6 0.27939267864370265 1105 7 0.32315467722394686 1105 8 0.3453561888461607 1105 9 0.14929790313574642 1105 10-11 0.3678549518653895 1105 12-13 0.4959732189213071 1105 14-15 0.6142338190582777 1105 16-17 0.5468152211922401 1105 18-19 0.5649950099755188 1105 20-21 0.7523652097559719 1105 22-23 0.4427515381485492 1105 24-25 0.6018151129348155 1105 26-27 0.4041860439172851 1105 28-29 0.5859833998375805 1105 30-31 0.6547098839265857 1105 32-33 0.49319112541466126 1105 34-35 0.6051596316250354 1105 36-37 0.5473128959801414 1105 38-39 0.8640109289543929 1105 40-41 0.7923616766275359 1105 42-43 0.6971428465845193 1105 44-45 0.7112758452469237 1105 46-47 0.752157880815119 1105 48-49 0.8190255032489802 1105 50-51 0.630027370700958 1105 52-53 0.7332160158828103 1105 54-55 0.7086699497154925 1105 56-57 0.8519775473427629 1105 58-59 1.0457371970119311 1105 60-61 1.1855238530630174 1105 62-63 1.0345586329497465 1105 64-65 1.2792675323346572 1105 66-67 1.193823446793381 1105 68-69 1.1431055461578907 1105 70-71 1.1200239949749076 1105 72-73 1.1414348598917634 1105 74-75 1.1187567548945445 1105 76-77 1.031932380176574 1105 78-79 0.9717422601136612 1105 80-81 0.8996145795042203 1105 82-83 0.8826086487899154 1105 84-85 0.9796567514618921 1105 86-87 1.0442520255540089 1105 88-89 0.8908817587571711 1105 90-91 1.2291264835269438 1105 92-93 1.0350673268850237 1105 94-95 1.088500854080234 1105 96-97 1.1603220556647749 1105 98-99 1.7662951525901427 1105 100-101 1.250507916784013 1106 1 0.0636449477636063 1106 2 0.12306913021208032 1106 3 0.05773695436059967 1106 4 -0.004810643559956418 1106 5 0.09531299796132231 1106 6 -0.21649193836767466 1106 7 -0.20294607141201482 1106 8 -0.12257089316543102 1106 9 0.06317516067497309 1106 10-11 -0.5180203600662665 1106 12-13 -0.5050587990121187 1106 14-15 -0.4742236524977841 1106 16-17 -0.3429090100812857 1106 18-19 -0.3521094183965161 1106 20-21 -0.2117235268679778 1106 22-23 -0.497006529778524 1106 24-25 -0.3418701215954911 1106 26-27 -0.4254738977144896 1106 28-29 -0.13664063264560866 1106 30-31 0.024916710471437398 1106 32-33 0.024029005548321436 1106 34-35 -0.29731678845701737 1106 36-37 -0.04222100411713825 1106 38-39 0.02268368621869854 1106 40-41 0.09581090049929486 1106 42-43 0.2200481462503845 1106 44-45 -0.015816740296365595 1106 46-47 0.3113254991376664 1106 48-49 0.7561400826170797 1106 50-51 0.5831234245012382 1106 52-53 0.34600838571743253 1106 54-55 0.6081306780494558 1106 56-57 0.1744514296758055 1106 58-59 0.24690033197788352 1106 60-61 -0.12569088279960994 1106 62-63 0.37756713440895595 1106 64-65 0.5607969490753391 1106 66-67 0.48811351150597915 1106 68-69 0.47253286079646983 1106 70-71 0.22631761253612837 1106 72-73 0.294901002090306 1106 74-75 0.5072797849668724 1106 76-77 0.5552902450837358 1106 78-79 0.62845249147178 1106 80-81 0.07461986648133845 1106 82-83 0.13624397425622803 1106 84-85 0.22796703357499482 1106 86-87 0.29768221630814296 1106 88-89 0.6779984529161176 1106 90-91 0.7775192424830841 1106 92-93 0.6797951533029902 1106 94-95 0.8137958674034138 1106 96-97 1.0449961663952188 1106 98-99 1.1740945012345634 1106 100-101 0.9441022153078862 1107 1 0.4210383000199691 1107 2 0.48371230763608253 1107 3 0.5772307632379743 1107 4 0.460731953831953 1107 5 0.47358339164266994 1107 6 0.5997283915512881 1107 7 0.5589785244166947 1107 8 0.41530602484847634 1107 9 0.6479328635833639 1107 10-11 0.767056669017613 1107 12-13 0.5292623371925771 1107 14-15 0.6804310021568725 1107 16-17 0.6083384702757826 1107 18-19 0.6556809061746307 1107 20-21 0.6212370021541957 1107 22-23 0.6650142238518129 1107 24-25 0.6567929250127591 1107 26-27 0.6918283360260986 1107 28-29 0.7673455453131695 1107 30-31 0.7884379371775978 1107 32-33 0.7127502610503953 1107 34-35 0.6318092553950478 1107 36-37 0.8941245880503743 1107 38-39 0.7087798851573623 1107 40-41 0.7749755103145901 1107 42-43 0.85783404859108 1107 44-45 0.9567731441085883 1107 46-47 1.044464460016357 1107 48-49 0.8425664062009375 1107 50-51 1.0075572761611085 1107 52-53 0.9340287691145264 1107 54-55 0.9120536078285539 1107 56-57 0.8204441689904556 1107 58-59 0.9421726590995263 1107 60-61 1.0611894907246011 1107 62-63 1.0507051697045213 1107 64-65 0.9769956820548913 1107 66-67 1.2211290613023564 1107 68-69 0.987409038343884 1107 70-71 1.2553654960360703 1107 72-73 1.2227029478717064 1107 74-75 1.1544937797836106 1107 76-77 1.033765295476595 1107 78-79 1.2260423758767338 1107 80-81 1.287485986025537 1107 82-83 1.528159005726053 1107 84-85 1.3651333094399014 1107 86-87 1.4422068779560924 1107 88-89 1.518749306509438 1107 90-91 1.4454997229635644 1107 92-93 1.59737197670173 1107 94-95 1.4855609544082249 1107 96-97 1.8609491056358323 1107 98-99 1.6983036418820596 1107 100-101 1.7192446522653917 1108 1 0.35969412193778183 1108 2 0.39993662270457264 1108 3 0.5761819618681052 1108 4 0.5841908579415431 1108 5 0.5107409258892446 1108 6 0.36539619977046556 1108 7 0.20728759975916233 1108 8 0.2204002029306693 1108 9 0.21978645947377373 1108 10-11 0.49496762792172433 1108 12-13 0.501651035822718 1108 14-15 0.4757435021568668 1108 16-17 0.5736638127415361 1108 18-19 0.5094052212431208 1108 20-21 0.4716543822911845 1108 22-23 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473.0 5 513.0 6 590.0 7 707.0 8 852.0 9 1057.0 10 1323.0 11 1774.0 12 2302.0 13 2927.0 14 3363.0 15 4139.0 16 5285.0 17 6499.0 18 5841.0 19 4420.0 20 4635.0 21 5167.0 22 5523.0 23 6043.0 24 7044.0 25 8162.0 26 9257.0 27 11142.0 28 13319.0 29 16441.0 30 19979.0 31 25213.0 32 32164.0 33 41802.0 34 56338.0 35 79369.0 36 112453.0 37 141081.0 38 86687.0 39 6342.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.1868798914545 18.07222324044414 14.685100434674379 23.055796433426977 2 15.562746859273297 20.859565869471 37.704989753075786 25.872697518179915 3 20.938116876370458 22.31472519781055 30.669931155913538 26.077226769905455 4 13.461044181099306 15.72150431201842 36.405247701806296 34.41220380507598 5 13.905313713775572 36.68823578468433 34.27808166552054 15.128368836019563 6 33.10206881098702 33.96869594681372 16.307564508592492 16.62167073360677 7 27.784213704334142 32.3130264671408 20.93887721618247 18.96388261234259 8 25.715944563220194 31.58994815609183 20.533739523637134 22.16036775705084 9 24.748912100448823 17.52635210372789 21.913883442749498 35.810852353073784 10-11 25.500543426655163 24.952198264996834 26.910993476830757 22.636264831517245 12-13 26.808807416656876 22.8994472481263 27.168861189058628 23.122884146158196 14-15 23.413451193228976 24.779132240579006 26.766856686146795 25.040559880045222 16-17 22.235032326541095 27.690247124215055 27.193703803371577 22.88101674587227 18-19 21.858111615656576 27.419427550368024 28.579517822112205 22.142943011863196 20-21 23.113000076493538 25.458619728775776 28.059195069445202 23.369185125285487 22-23 22.592223752226705 25.046036741964745 28.8657202496093 23.49601925619925 24-25 24.060171945213042 25.392552683578874 27.836423233572688 22.710852137635403 26-27 23.027507520306877 24.78354357406512 29.631716706419787 22.557232199208215 28-29 22.074553475669152 27.10556049682279 28.3635342675744 22.456351759933654 30-31 22.253121270681813 25.486660013238545 28.26395392603492 23.996264790044723 32-33 22.60144173095158 25.729404917078607 28.576727904708033 23.09242544726178 34-35 24.16551166353922 25.238588694335284 27.564602188232833 23.031297453892662 36-37 22.95889793465195 24.757062069719158 29.216547371871926 23.067492623756966 38-39 22.680959398607147 25.874288242982818 28.680615214834866 22.764137143575166 40-41 22.836656161544425 26.16888548961495 28.206355655864485 22.788102692976135 42-43 22.491363424632645 26.084370018345464 26.883825254999245 24.540441302022646 44-45 23.100886275838327 25.29349007993968 27.30086114101184 24.304762503210153 46-47 23.839036354583374 26.26181009994488 27.289398930542646 22.609754614929102 48-49 23.472957436474413 25.938153879574465 27.24456385485214 23.344324829098976 50-51 21.877589385679027 26.601790138594684 28.52383583952281 22.99678463620348 52-53 23.243588920211145 26.839560055150248 26.224175649989956 23.69267537464865 54-55 22.413088884365767 25.49925482315881 27.40085596298568 24.686800329489742 56-57 23.511910249240138 25.945106940606156 26.792546627961684 23.750436182192022 58-59 24.752010751322402 25.31115599933897 26.790487155638775 23.14634609369985 60-61 23.530054420932597 25.425227550511774 28.3973479184164 22.647370110139228 62-63 22.44108091276957 26.094357943441498 28.278293931030152 23.18626721275878 64-65 22.383273710064984 26.99667388883237 27.257245439144317 23.36280696195833 66-67 22.914593933153107 25.88574148264435 26.504335071641183 24.69532951256136 68-69 22.31518956483499 25.551049450538194 27.77750842817664 24.356252556450176 70-71 23.71060876310765 25.737497370958444 26.76412102605005 23.787772839883857 72-73 23.116076818232724 24.860151702625707 28.555615646620836 23.468155832520736 74-75 22.355863466926014 27.32109367423347 26.720083704297632 23.602959154542884 76-77 23.01541394897164 25.75750022365284 26.85090652813621 24.376179299239308 78-79 23.00555091158425 25.795075205017675 27.384474384400626 23.81489949899745 80-81 23.67182879876972 25.728669158730437 26.765283921818213 23.834218120681623 82-83 22.986205559287963 25.40624095345776 27.9712258508529 23.636327636401376 84-85 22.922195541760914 26.102457978273442 27.24961919463848 23.725727285327164 86-87 22.90002376094847 26.393907365074682 27.09116830125605 23.614900572720792 88-89 22.6733729281353 25.899315944942376 26.0209955329525 25.406315593969826 90-91 22.866757624551525 25.015193790826913 27.062429067190013 25.055619517431545 92-93 23.70038108907761 25.561305767821768 27.405227905744468 23.33308523735615 94-95 23.016927625693935 25.24455946519901 27.321283467739182 24.417229441367866 96-97 21.579091490775397 25.51271886965233 28.4352147194856 24.47297492008667 98-99 22.31787238227404 25.93037518234616 26.74021067469445 25.011541760685347 100-101 22.098413489605917 24.537139774740787 27.993390393537883 25.371056342115416 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1026.0 1 619.5 2 210.5 3 203.0 4 179.0 5 170.5 6 261.0 7 493.0 8 750.5 9 893.5 10 837.5 11 770.0 12 818.0 13 860.5 14 890.0 15 890.0 16 922.5 17 953.5 18 922.0 19 944.5 20 1061.0 21 1184.5 22 1343.0 23 1542.0 24 1630.5 25 1925.5 26 2388.0 27 2787.0 28 3273.0 29 3805.5 30 4589.0 31 5846.5 32 7051.0 33 7541.0 34 8315.0 35 9669.0 36 11131.0 37 12498.5 38 13954.5 39 15119.0 40 15747.0 41 17768.5 42 20185.0 43 22353.5 44 23933.5 45 24883.5 46 27188.0 47 36618.5 48 52676.0 49 52503.5 50 38688.0 51 31130.0 52 28453.0 53 28814.5 54 29314.5 55 26157.0 56 22042.5 57 18908.0 58 16509.5 59 14571.0 60 12250.0 61 10014.5 62 7920.5 63 6030.0 64 4603.5 65 3557.5 66 2562.5 67 2002.5 68 1596.0 69 1211.0 70 898.0 71 624.0 72 499.5 73 418.5 74 328.5 75 257.5 76 183.0 77 117.0 78 64.5 79 43.5 80 30.5 81 20.0 82 10.0 83 6.0 84 7.0 85 5.5 86 3.5 87 3.0 88 3.5 89 2.5 90 1.0 91 2.0 92 1.0 93 1.5 94 2.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32202474764275707 2 0.2788063543308375 3 0.2693991961651515 4 0.2781246762028892 5 0.27771566932612024 6 0.21350158967339436 7 0.20204939712386363 8 0.20164039024709468 9 0.2139105965501633 10-11 0.2119337299791134 12-13 0.20586679464037388 14-15 0.2101613668464479 16-17 0.19400559521407418 18-19 0.20068604086796712 20-21 0.1903245333231536 22-23 0.19905001336089131 24-25 0.19748215366661034 26-27 0.1998680271144292 28-29 0.21172922654072893 30-31 0.20866167496496174 32-33 0.2162283021851874 34-35 0.20770732558583418 36-37 0.1914152183278708 38-39 0.17955401890157113 40-41 0.17744081670493153 42-43 0.19400559521407418 44-45 0.19523261584438106 46-47 0.1950281124059966 48-49 0.2119337299791134 50-51 0.2085253393393721 52-53 0.22699881660676988 54-55 0.19673230772586722 56-57 0.17430509731636953 58-59 0.17655463513859881 60-61 0.1675564838496818 62-63 0.17055586761265412 64-65 0.17007869292309036 66-67 0.17157838480457652 68-69 0.17280540543488337 70-71 0.17423692950357472 72-73 0.17205555949414028 74-75 0.18855217019048814 76-77 0.1803038648423142 78-79 0.18432576579720894 80-81 0.17628196388741948 82-83 0.15814932568399584 84-85 0.15739947974325275 86-87 0.1622393944516854 88-89 0.16810182635204038 90-91 0.17410059387798507 92-93 0.16912434354396277 94-95 0.17028319636147482 96-97 0.17512311106990744 98-99 0.18541645080192615 100-101 0.16585228852981115 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 733484.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.67419268553384 #Duplication Level Percentage of deduplicated Percentage of total 1 66.8486889593749 25.184703886308085 2 15.127857992423083 11.398596738518807 3 6.410654571509153 7.245487067023026 4 3.4108583477380794 5.14005338462984 5 2.053909917984827 3.868969900444468 6 1.2860623619532232 2.90708167379031 7 0.9179704566774228 2.4208657105144913 8 0.6132406362742004 1.8482676714874882 9 0.4809444145337888 1.6307273289759499 >10 2.520333617746369 17.72263027339249 >50 0.22244365242452815 5.764223480043492 >100 0.09538499556607474 5.9893002232751265 >500 0.0032765838171551626 0.8060805050080704 >1k 0.007645362240028712 5.9573422927548965 >5k 7.281297371455916E-4 2.115669863833501 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8630 1.1765764488386932 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6795 0.9264005758816825 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4688 0.6391414127642866 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3144 0.4286392068538646 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2646 0.3607440653102181 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2609 0.35569964716340097 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2600 0.3544726265330941 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2474 0.337294337708798 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2405 0.32788717954311203 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2367 0.3227064257707053 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2285 0.31152690447235387 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2220 0.3026650888090265 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2082 0.28385077247765456 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1798 0.24513145481019355 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1722 0.23476994726538003 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1586 0.2162283021851874 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1527 0.20818450027539795 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1442 0.1965959721002776 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1323 0.18037203265510904 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1237 0.168647168854399 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1178 0.16060336694460955 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1089 0.14846949626713057 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1012 0.1379716530967274 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 850 0.11588528175120384 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 808 0.11015918547643848 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 755 0.10293339732018694 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.3633562558965158E-4 0.0 10-11 0.0 0.0 0.0 1.3633562558965158E-4 0.0 12-13 0.0 0.0 0.0 1.3633562558965158E-4 0.0 14-15 0.0 0.0 0.0 2.0450343838447735E-4 0.0 16-17 0.0 0.0 0.0 2.7267125117930315E-4 0.0 18-19 0.0 0.0 0.0 2.7267125117930315E-4 0.0 20-21 0.0 0.0 0.0 2.7267125117930315E-4 0.0 22-23 0.0 0.0 0.0 6.816781279482579E-4 0.0 24-25 0.0 0.0 0.0 8.861815663327353E-4 0.0 26-27 0.0 0.0 0.0 0.0015678596942809932 0.0 28-29 0.0 0.0 0.0 0.004567243457253328 0.0 30-31 0.0 0.0 0.0 0.011043185672761777 0.0 32-33 0.0 0.0 0.0 0.022222706971113206 0.0 34-35 0.0 0.0 0.0 0.03210703982636295 0.0 36-37 0.0 0.0 0.0 0.03878748548025587 0.0 38-39 0.0 0.0 0.0 0.04417274269104711 0.0 40-41 0.0 0.0 0.0 0.0515348664728883 0.0 42-43 0.0 0.0 0.0 0.06066935338739496 0.0 44-45 0.0 0.0 0.0 0.06857681967159475 0.0 46-47 0.0 0.0 0.0 0.0809151937874582 0.0 48-49 0.0 0.0 0.0 0.09482142759760268 0.0 50-51 0.0 0.0 0.0 0.11077269579159191 0.0 52-53 0.0 0.0 0.0 0.14499293781459446 0.0 54-55 0.0 0.0 0.0 0.1947554411548173 0.0 56-57 0.0 0.0 0.0 0.2640139389543603 0.0 58-59 0.0 0.0 0.0 0.34738317400243224 0.0 60-61 0.0 0.0 0.0 0.4717894323529893 0.0 62-63 0.0 0.0 0.0 0.5588397292919818 0.0 64-65 0.0 0.0 0.0 0.6471170468612812 0.0 66-67 0.0 0.0 0.0 0.804925533481303 0.0 68-69 0.0 0.0 0.0 1.000567156202453 0.0 70-71 0.0 0.0 0.0 1.1647834172251883 0.0 72-73 0.0 0.0 0.0 1.325386784169798 0.0 74-75 0.0 0.0 0.0 1.6215077629505212 0.0 76-77 0.0 0.0 0.0 1.8356910307518637 0.0 78-79 0.0 0.0 0.0 2.0497379629276167 0.0 80-81 0.0 0.0 0.0 2.2525372059922235 0.0 82-83 0.0 0.0 0.0 2.547499331955435 0.0 84-85 0.0 0.0 0.0 2.868637898031859 0.0 86-87 0.0 0.0 0.0 3.2871201007792945 0.0 88-89 0.0 0.0 0.0 3.839074880978999 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGCG 80 4.4618815E-4 29.721691 5 ATACCGT 70 0.008370505 27.15555 6 TAGACTG 155 1.3954086E-6 24.544365 5 GAGTACT 2300 0.0 24.165258 12-13 TAAGGTG 200 2.0265361E-8 23.777351 5 GTACTTT 2485 0.0 22.077982 14-15 GTGCTAG 285 8.185452E-11 21.69904 1 CTAGTCA 110 0.0028560956 21.617252 4 TCAATAC 200 5.328329E-7 21.39669 3 TACATGG 2025 0.0 21.368801 2 ATAAGGC 245 7.4196578E-9 21.348171 3 GTACATG 2125 0.0 21.26702 1 GGTAAGG 205 6.756072E-7 20.87482 3 ACATGGG 2030 0.0 20.846258 3 GTATATA 235 1.11278496E-7 20.242968 1 AGTACTT 2355 0.0 19.969982 12-13 GTAAGCG 145 2.4519977E-9 19.64758 94-95 ACTTTTT 2935 0.0 19.095594 16-17 CTATTAG 125 0.005977324 19.028387 1 CATGGGC 475 0.0 19.023182 4 >>END_MODULE