FastQCFastQC Report
Thu 26 May 2016
SRR937854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937854_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733484
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT109081.4871490039319195No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55270.7535270026340043No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46100.6285072339682938No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC34520.47063057953547727No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30060.40982489052249266No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28740.39182858794465864No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA26500.3612894078125767No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26170.3567903321681182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24780.3378396802111566No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24740.337294337708798No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23820.3247514601545501No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22650.3088001919605608No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20790.28344176560088563No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA17810.24281374917516949No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC16990.23163422787681803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14990.20436710275888773No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT14740.20095871211914643No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA14110.1923695677069984No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC13700.18677980705782266No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG12130.16537511384024736No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG11120.15160521565569254No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC10620.14478843437620997No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT10000.1363356255896516No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG7700.10497843170403172No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC7450.10157004106429042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGT651.3267464E-436.5431
TAAGGTG2150.033.1413885
GTAAGGT2050.032.440844
ACTCCGT856.4079615E-427.938936
GGTAAGG2253.6379788E-1227.445983
TAAGGCC2700.026.3903664
ATAAGGC3000.025.3347533
CCTATAA4350.025.1180611
AGGTAAG2850.025.00142
AAGGTAA2701.8189894E-1224.632691
CATAAGG2602.3646862E-1123.751332
TACATGG19450.022.7133542
GAGTACT26750.022.63991212-13
GCATAAG2755.0931703E-1122.4573351
CTATAAG4450.022.4169862
GTACATG20050.022.0351541
GCGGTCG1302.940346E-421.922819
AGTACTT26950.021.85502212-13
CATGGGG12500.021.6612134
GTCTAGA1100.002874312521.593591
Produced by FastQC (version 0.11.5)