##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937854_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 733484 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19531032715097 33.0 31.0 34.0 31.0 34.0 2 32.40357935551423 34.0 31.0 34.0 31.0 34.0 3 32.49483696985892 34.0 31.0 34.0 31.0 34.0 4 36.11046730399027 37.0 35.0 37.0 35.0 37.0 5 36.0122415758217 37.0 35.0 37.0 35.0 37.0 6 36.053080912467074 37.0 35.0 37.0 35.0 37.0 7 36.00735530700056 37.0 35.0 37.0 35.0 37.0 8 35.99155264463847 37.0 35.0 37.0 35.0 37.0 9 37.65105441972831 39.0 37.0 39.0 35.0 39.0 10-11 37.58730524455885 39.0 37.0 39.0 35.0 39.0 12-13 37.50246903817943 39.0 37.0 39.0 35.0 39.0 14-15 38.69184876561725 40.0 38.0 41.0 34.0 41.0 16-17 38.669325438591706 40.0 38.0 41.0 34.5 41.0 18-19 38.64542103167895 40.0 38.0 41.0 34.0 41.0 20-21 38.51842575979844 40.0 38.0 41.0 34.0 41.0 22-23 38.362857267506854 40.0 38.0 41.0 34.0 41.0 24-25 38.40975535935344 40.0 38.0 41.0 34.0 41.0 26-27 38.272212481799194 40.0 38.0 41.0 34.0 41.0 28-29 38.15025208457172 40.0 38.0 41.0 34.0 41.0 30-31 38.263976446657324 40.0 38.0 41.0 34.0 41.0 32-33 38.19256725436411 40.0 38.0 41.0 34.0 41.0 34-35 38.04202954665678 40.0 37.0 41.0 33.0 41.0 36-37 37.87819843377633 40.0 37.0 41.0 33.0 41.0 38-39 37.73720626489467 40.0 37.0 41.0 33.0 41.0 40-41 37.60872152630459 40.0 37.0 41.0 33.0 41.0 42-43 37.38598115296312 40.0 37.0 41.0 32.5 41.0 44-45 37.10678760545561 40.0 36.0 41.0 31.5 41.0 46-47 36.79251081141511 39.0 36.0 41.0 31.0 41.0 48-49 36.47733692895823 39.0 36.0 41.0 30.0 41.0 50-51 36.19358295477475 39.0 35.0 40.5 30.0 41.0 52-53 35.98976051284009 39.0 35.0 40.0 29.0 41.0 54-55 35.77834008649132 39.0 35.0 40.0 29.0 41.0 56-57 35.45145701883067 38.0 35.0 40.0 28.0 41.0 58-59 35.16740378794903 38.0 34.0 40.0 28.0 41.0 60-61 34.71286694733627 37.5 34.0 40.0 27.0 41.0 62-63 34.369476362129234 37.0 34.0 40.0 26.0 41.0 64-65 33.967801615304495 36.5 33.0 39.0 26.0 40.5 66-67 33.45924450976436 36.0 33.0 39.0 25.5 40.0 68-69 32.99106456309885 35.5 32.0 38.5 24.5 40.0 70-71 32.493862170135955 35.0 32.0 37.5 23.5 39.5 72-73 31.96978734369121 35.0 31.5 37.0 22.5 39.0 74-75 31.47584950728305 34.5 31.0 36.5 20.5 39.0 76-77 30.25665454188503 33.5 29.5 35.0 20.0 37.0 78-79 30.877668088192788 34.0 31.0 35.0 20.0 37.0 80-81 30.681180502914856 34.0 31.0 35.0 20.0 37.0 82-83 30.352581651406165 34.0 30.5 35.0 18.5 36.0 84-85 30.004673585245214 34.0 30.0 35.0 17.5 36.0 86-87 29.687290383975657 34.0 30.0 35.0 12.5 35.5 88-89 29.34156914124916 34.0 29.5 35.0 7.0 35.0 90-91 28.843121322346498 34.0 29.0 35.0 2.0 35.0 92-93 28.41628242742855 33.0 29.0 35.0 2.0 35.0 94-95 27.92819202600193 33.0 27.0 35.0 2.0 35.0 96-97 27.36577144150384 33.0 27.0 35.0 2.0 35.0 98-99 26.54674948601469 33.0 25.0 34.0 2.0 35.0 100-101 23.960624907973454 30.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29003598172587175 1101 2 0.27514425010627264 1101 3 0.06932070380213418 1101 4 0.05695565792851909 1101 5 0.0387452802811481 1101 6 -0.055307386021581806 1101 7 0.1881193880330656 1101 8 0.10444259584811988 1101 9 0.07317937357820625 1101 10-11 0.1585104080821509 1101 12-13 0.16493218326923653 1101 14-15 0.19770764595153167 1101 16-17 0.25582523056727524 1101 18-19 0.3188051617917296 1101 20-21 0.22520343727983771 1101 22-23 0.1302673321395318 1101 24-25 0.2572377634108989 1101 26-27 0.11973962807204686 1101 28-29 0.25683820204769603 1101 30-31 0.1512237695294445 1101 32-33 0.2987756890287301 1101 34-35 0.19215399027759617 1101 36-37 0.16413508662967047 1101 38-39 0.04246340958425776 1101 40-41 0.046567860753569335 1101 42-43 0.008421307585159354 1101 44-45 0.06019762807994056 1101 46-47 0.18199620816412931 1101 48-49 -0.09233528096473265 1101 50-51 -0.21274015715552963 1101 52-53 -0.24015734349059414 1101 54-55 0.04226600458227381 1101 56-57 0.10265276105250365 1101 58-59 0.004508703924244628 1101 60-61 -0.06185888510893278 1101 62-63 0.1143041670724898 1101 64-65 0.33041180174385687 1101 66-67 0.11592979358628241 1101 68-69 0.07892996088320103 1101 70-71 0.11895481103132255 1101 72-73 -0.06256501570264916 1101 74-75 0.028799796519987098 1101 76-77 -0.11581732668075517 1101 78-79 -0.028565572430053976 1101 80-81 0.020030665559030325 1101 82-83 0.07490050180826913 1101 84-85 0.09394305420948257 1101 86-87 0.2029785130826376 1101 88-89 0.24944620557505814 1101 90-91 0.24181644323711993 1101 92-93 0.4683761303318761 1101 94-95 0.49946661581588003 1101 96-97 0.6646890112928432 1101 98-99 0.6959425413265983 1101 100-101 0.8699938808804148 1102 1 0.2667284881858194 1102 2 0.25250536121738776 1102 3 0.21307716375045516 1102 4 0.1385157354479034 1102 5 0.175873833252723 1102 6 0.16691483620063963 1102 7 0.06788682989353134 1102 8 0.11604789300574225 1102 9 0.1377013374024969 1102 10-11 0.18648844425786137 1102 12-13 0.2921640954139306 1102 14-15 0.3336847131349927 1102 16-17 0.36349318922361107 1102 18-19 0.3403975390527094 1102 20-21 0.3495138377966285 1102 22-23 0.33858290061498764 1102 24-25 0.11565184609823831 1102 26-27 0.1883071345319962 1102 28-29 0.21064957155673625 1102 30-31 0.2484040020875895 1102 32-33 0.28634028851193705 1102 34-35 0.017804830070872413 1102 36-37 0.19286893675886319 1102 38-39 0.07601315118633067 1102 40-41 0.27580881682591496 1102 42-43 0.35799656598309326 1102 44-45 0.3846889071497088 1102 46-47 0.45577850790573393 1102 48-49 0.4055458559861762 1102 50-51 0.3975166257901961 1102 52-53 0.2974427857083768 1102 54-55 0.3617233689233501 1102 56-57 -0.0657419417898808 1102 58-59 0.03769345844620631 1102 60-61 0.04206553349571607 1102 62-63 0.17297503270555836 1102 64-65 0.25545540639502207 1102 66-67 0.22402572381883346 1102 68-69 -0.017586511985015818 1102 70-71 -0.03414757914955402 1102 72-73 0.29303414450407317 1102 74-75 0.026017173780967795 1102 76-77 0.06000147176886017 1102 78-79 0.19838210198854966 1102 80-81 0.3744525001843577 1102 82-83 0.22257815038707562 1102 84-85 0.2506872402559921 1102 86-87 0.2403331642454276 1102 88-89 0.30056216164740945 1102 90-91 0.05863007372807871 1102 92-93 0.27463259028019493 1102 94-95 0.5927224297693705 1102 96-97 0.8133566469517568 1102 98-99 0.6146263268563139 1102 100-101 0.935930573386873 1103 1 0.34417479920659133 1103 2 0.3341963066615463 1103 3 0.3470488529023896 1103 4 0.17069923119330355 1103 5 0.045123814985515764 1103 6 0.1644869584514268 1103 7 0.28860781990967155 1103 8 0.19400236448564812 1103 9 0.28806369234427365 1103 10-11 0.2906248039690382 1103 12-13 0.22719760743375872 1103 14-15 0.38257107782813904 1103 16-17 0.5536979812099574 1103 18-19 0.34770940343697276 1103 20-21 0.3195655838093998 1103 22-23 0.5632413038619006 1103 24-25 0.44411115672709656 1103 26-27 0.27475087485868244 1103 28-29 0.17875336914281803 1103 30-31 0.3706614301978206 1103 32-33 0.3889106504683184 1103 34-35 0.5305553398919898 1103 36-37 0.5719499966553698 1103 38-39 0.37469027333747107 1103 40-41 0.3586854700080693 1103 42-43 0.2418387240376063 1103 44-45 0.33024100854266436 1103 46-47 0.45532847037492985 1103 48-49 0.21668785528206058 1103 50-51 0.5908218608393199 1103 52-53 0.207551525404007 1103 54-55 0.3838084210347148 1103 56-57 0.3579708844458196 1103 58-59 0.44563338258748075 1103 60-61 0.38552222028952343 1103 62-63 0.4967961336534614 1103 64-65 0.5816794752657088 1103 66-67 0.5536724028407747 1103 68-69 0.5013897937880785 1103 70-71 0.7168870089747656 1103 72-73 0.45830420023565566 1103 74-75 0.31096399960481946 1103 76-77 0.09714700493878681 1103 78-79 0.3054993375956556 1103 80-81 0.1307729534767681 1103 82-83 0.24870191569001676 1103 84-85 0.45517698737143775 1103 86-87 0.6244277418744097 1103 88-89 0.5845956271688912 1103 90-91 0.4517087954222134 1103 92-93 0.6078330712059135 1103 94-95 0.7500578754559832 1103 96-97 0.8632510292877029 1103 98-99 1.0206581582931769 1103 100-101 1.23264812252566 1104 1 0.20247800082545808 1104 2 0.19391812459467417 1104 3 -0.07833789101368893 1104 4 -0.0881517772692959 1104 5 -0.07185328724955298 1104 6 0.10431062216395759 1104 7 0.014490465795688579 1104 8 0.03910664768986294 1104 9 0.12260638585651407 1104 10-11 0.08084669730452276 1104 12-13 0.1669257030509499 1104 14-15 0.12311948087648972 1104 16-17 0.06637434380056817 1104 18-19 -0.010125602191898508 1104 20-21 -0.055230566812930704 1104 22-23 -0.04636039637342293 1104 24-25 -0.050683450777370354 1104 26-27 -0.12150951926764719 1104 28-29 -0.06086302578313507 1104 30-31 0.20380391959765376 1104 32-33 0.13890188275315296 1104 34-35 0.05098499300611081 1104 36-37 0.06976946589296773 1104 38-39 0.021544241780709683 1104 40-41 0.09927420454619806 1104 42-43 0.2596687496042591 1104 44-45 0.200609463004902 1104 46-47 -0.024148061958655376 1104 48-49 -0.020548104975645742 1104 50-51 -0.05799169194407483 1104 52-53 -0.063965324390999 1104 54-55 -0.06493726963328328 1104 56-57 0.21100030539084003 1104 58-59 0.1916949249337918 1104 60-61 -0.07921052221671232 1104 62-63 0.029303314412182146 1104 64-65 0.15334239519542336 1104 66-67 0.19125977312243236 1104 68-69 -0.016497221927167516 1104 70-71 0.09119304431917641 1104 72-73 0.009853981568834769 1104 74-75 -0.08439682694521977 1104 76-77 0.054248328162355364 1104 78-79 -0.08037832822814295 1104 80-81 -0.1387159237997686 1104 82-83 0.24413481285874283 1104 84-85 0.049863927333628766 1104 86-87 0.003174624951665095 1104 88-89 0.11481881812622063 1104 90-91 0.24589983341447663 1104 92-93 0.11009338817634884 1104 94-95 0.12267261854439226 1104 96-97 0.44908959110184554 1104 98-99 0.37686938307284734 1104 100-101 0.3103519982064711 1105 1 0.3046838690498177 1105 2 0.22871819376824476 1105 3 0.10226084464720486 1105 4 0.1390507283510587 1105 5 0.12034739295719987 1105 6 0.08835458580940525 1105 7 0.13473910634292707 1105 8 0.3042489338762877 1105 9 0.3479681059725763 1105 10-11 0.28467237991313254 1105 12-13 0.30230894383260676 1105 14-15 0.19771644876842487 1105 16-17 0.29494494887713785 1105 18-19 0.29107628855229706 1105 20-21 0.42739533868828516 1105 22-23 0.2765437405902347 1105 24-25 0.2611990310165311 1105 26-27 0.17837519145233216 1105 28-29 0.13465510345614717 1105 30-31 0.21057236107873933 1105 32-33 0.29789540733858644 1105 34-35 0.2787649057705579 1105 36-37 0.23188156550290984 1105 38-39 0.28667115606312876 1105 40-41 0.4227826494859599 1105 42-43 0.17269067378234126 1105 44-45 0.29985431822078823 1105 46-47 0.3693025461923014 1105 48-49 0.4237210570634389 1105 50-51 0.24784963157686235 1105 52-53 0.49176167059390963 1105 54-55 0.27170262430057335 1105 56-57 0.10414923992573932 1105 58-59 0.32496645040311734 1105 60-61 0.35284181911642065 1105 62-63 0.2681509980584025 1105 64-65 0.481036801743862 1105 66-67 0.4550935259806437 1105 68-69 0.2814035524324936 1105 70-71 0.43715023356653404 1105 72-73 0.16587688570579573 1105 74-75 0.22951282468900303 1105 76-77 0.41297668740375215 1105 78-79 0.414726681091075 1105 80-81 0.42420320077030027 1105 82-83 0.46386176941389934 1105 84-85 0.4309853077306087 1105 86-87 0.49708062575869505 1105 88-89 0.3193669802229486 1105 90-91 0.47899073901177047 1105 92-93 0.5412810599093412 1105 94-95 0.6722835172243329 1105 96-97 0.4914055605886176 1105 98-99 0.6769020483688486 1105 100-101 0.42883190904942836 1106 1 -2.943486044628685E-4 1106 2 0.16391677262879512 1106 3 0.25030043353186215 1106 4 0.06637382609668663 1106 5 0.1121123973982634 1106 6 0.09484276412857184 1106 7 0.0686899586036418 1106 8 -0.00784344018791927 1106 9 0.012308502707334412 1106 10-11 0.1572266242983602 1106 12-13 0.11376476585181905 1106 14-15 0.1761667300356109 1106 16-17 0.13084775308980312 1106 18-19 0.09251011674668774 1106 20-21 0.09890276160415823 1106 22-23 0.008741431238632913 1106 24-25 0.1049667423898697 1106 26-27 1.1312657054673991E-4 1106 28-29 0.13183820204769603 1106 30-31 0.013209505265173505 1106 32-33 0.09475917251221233 1106 34-35 -0.06678557428696763 1106 36-37 0.07954049203507196 1106 38-39 0.0061758720467182115 1106 40-41 0.07024278567848796 1106 42-43 -0.007601590312738438 1106 44-45 0.012665721173028999 1106 46-47 0.12312608804401037 1106 48-49 0.19556261693316657 1106 50-51 0.299814272273899 1106 52-53 0.12290196581871982 1106 54-55 0.18692065923691814 1106 56-57 0.32463098928073464 1106 58-59 0.14026696218250123 1106 60-61 0.17772444822439581 1106 62-63 0.051824812718130886 1106 64-65 0.04274851345557096 1106 66-67 0.2239384272199132 1106 68-69 0.2945512071294445 1106 70-71 0.260548279499794 1106 72-73 0.28603295727032574 1106 74-75 -0.0011495278043334167 1106 76-77 0.17444731295888616 1106 78-79 0.37014689003240875 1106 80-81 0.48215904393741127 1106 82-83 0.3528246759824434 1106 84-85 -0.007588477322048703 1106 86-87 0.37803481938894024 1106 88-89 0.5116627971666503 1106 90-91 0.4369909927866722 1106 92-93 0.28933333753908386 1106 94-95 0.7338810302302967 1106 96-97 0.814735933214763 1106 98-99 0.6332379617470174 1106 100-101 0.7124338208203547 1107 1 0.4053784474792934 1107 2 0.42906548298298475 1107 3 0.3647487859939176 1107 4 0.16190428806550727 1107 5 0.24499528028114526 1107 6 0.3196926139784182 1107 7 0.25496870310156083 1107 8 0.25250766434126604 1107 9 0.4020149900165677 1107 10-11 0.3553939697259878 1107 12-13 0.31799725176238525 1107 14-15 0.4610894952665987 1107 16-17 0.4071266004302956 1107 18-19 0.4012537919287169 1107 20-21 0.38416747837572274 1107 22-23 0.46272452392035746 1107 24-25 0.6071521469725454 1107 26-27 0.47678586094875897 1107 28-29 0.5265299828696115 1107 30-31 0.4743915777486265 1107 32-33 0.3878167849191385 1107 34-35 0.44384063411321506 1107 36-37 0.436977552383091 1107 38-39 0.5685421767075454 1107 40-41 0.35422197034260705 1107 42-43 0.4373853486810475 1107 44-45 0.49833975136761666 1107 46-47 0.6319448383011164 1107 48-49 0.6093085546517045 1107 50-51 0.47398073325542356 1107 52-53 0.5327707387011884 1107 54-55 0.8875742237603532 1107 56-57 0.5005123500936008 1107 58-59 0.733036101184517 1107 60-61 0.839254128589701 1107 62-63 0.7032682081683745 1107 64-65 0.8909683085931732 1107 66-67 0.9028047935862773 1107 68-69 0.9173118101982709 1107 70-71 0.9008126877436453 1107 72-73 1.0519298473110474 1107 74-75 0.8214624677528626 1107 76-77 0.7976501390726689 1107 78-79 0.9205268933233697 1107 80-81 0.9188491587097154 1107 82-83 0.9894381730411439 1107 84-85 0.8167512733875668 1107 86-87 1.1402216637675657 1107 88-89 1.1109958631093058 1107 90-91 1.0398387035110943 1107 92-93 1.3235644864962595 1107 94-95 1.8862474377336902 1107 96-97 1.8200400386901023 1107 98-99 1.6563106920115267 1107 100-101 1.8401308671817844 1108 1 0.08159848172586948 1108 2 0.18201925010627207 1108 3 0.25650820380213446 1108 4 0.1138306579285242 1108 5 0.0699952802811481 1108 6 0.11969261397841535 1108 7 0.09968188803306788 1108 8 0.07506759584811817 1108 9 0.07380437357820568 1108 10-11 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88-89 0.15435956047339516 2316 90-91 0.21230281107445848 2316 92-93 0.27066587155183797 2316 94-95 0.16188805759037095 2316 96-97 0.2777493162836002 2316 98-99 0.6337972086094048 2316 100-101 1.066856172932173 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 4.0 5 18.0 6 38.0 7 100.0 8 192.0 9 378.0 10 608.0 11 972.0 12 1519.0 13 1933.0 14 2574.0 15 3555.0 16 4785.0 17 6426.0 18 5923.0 19 3985.0 20 3625.0 21 3949.0 22 3951.0 23 4268.0 24 5094.0 25 5712.0 26 6996.0 27 8561.0 28 11239.0 29 13886.0 30 17772.0 31 22232.0 32 28429.0 33 38041.0 34 52630.0 35 76786.0 36 118229.0 37 167163.0 38 106070.0 39 5838.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.927030550298085 18.81944179298232 14.982382170538408 22.271145486181183 2 16.10519111527995 21.940901233019396 36.390977853640976 25.56292979805967 3 21.629919671049404 22.779774337272524 30.198068396856648 25.392237594821427 4 14.233711982810803 16.383588462734018 35.49934286228466 33.883356692170516 5 14.064000043637634 37.576502225519285 33.557748952696805 14.801748778146273 6 33.39759285819459 34.9414574823718 15.466322373766845 16.194627285666762 7 28.519776845848032 32.63806708803464 20.07064912118056 18.771506944936768 8 26.249652344154743 32.13676099274149 19.66191491566278 21.95167174744098 9 24.84048731806011 18.001210660355234 21.18096645598268 35.977335565601976 10-11 25.96634691417945 25.43947788908824 26.284554264305697 22.30962093242661 12-13 27.350483867009512 23.10147982319562 26.62556955605304 22.922466753741823 14-15 23.569702951938964 25.269535531790744 26.446793658757382 24.713967857512912 16-17 22.407237240348802 28.007291229256538 26.882999492831473 22.702472037563194 18-19 22.01438613521222 27.816148682179843 28.284325220454708 21.88513996215323 20-21 23.36042776665885 25.51937056568378 28.019288764308424 23.10091290334895 22-23 22.95404147235801 25.36768389989318 28.426813947004437 23.251460680744373 24-25 24.386966859536134 25.780385120875167 27.765977171962852 22.06667084762585 26-27 23.431847385588615 25.003459523574183 29.458576039646484 22.106117051190726 28-29 22.309697509260687 27.593230598914488 28.123104064612797 21.973967827212032 30-31 22.700699674430528 25.745415032911417 27.973957168799863 23.579928123858192 32-33 22.94228640297539 26.122246702041217 28.429249990456505 22.506216904526887 34-35 24.46535984425018 25.5124856165915 27.527185323742575 22.494969215415743 36-37 23.32723004182777 24.99720511967541 28.94909773082985 22.72646710766697 38-39 22.90474155178678 26.17257238915395 28.552653195334322 22.37003286372495 40-41 23.130471771233182 26.441807246409414 27.811509644769018 22.61621133758839 42-43 22.683063514377693 26.61656165248373 26.574769598291777 24.1256052348468 44-45 23.64448304257489 25.468926384215607 27.041694161017826 23.844896412191677 46-47 24.177350835192044 26.695060832956134 26.9129251626484 22.21466316920342 48-49 23.9855947778002 26.382579579104657 26.724577495896295 22.907248147198846 50-51 22.220116594227004 27.035422722240703 28.056644725719988 22.687815957812305 52-53 23.81047166673029 27.4781726663431 25.516030342856833 23.19532532406978 54-55 22.891910389320014 26.216454619323663 26.661113262184315 24.230521729172004 56-57 23.937604637592642 26.488648695813406 26.368196170604946 23.205550495989005 58-59 25.32400161421381 25.714535013715363 26.079164644354886 22.88229872771594 60-61 23.83208086338625 26.258854998882047 27.79862955429157 22.11043458344013 62-63 22.74384989992965 26.601943600678407 27.735983334333124 22.918223165058816 64-65 22.88011735770651 27.656295161175976 26.664589820636852 22.798997660480666 66-67 23.334523997796815 26.396758484165982 26.044808066706292 24.223909451330908 68-69 22.770519036897216 26.220903401371675 26.96884115320931 24.0397364085218 70-71 24.185121965850655 26.502145922746784 26.118429304524703 23.194302806877857 72-73 23.730101815445188 25.301369900365927 27.889633584372664 23.078894699816217 74-75 22.697563955041964 28.316636763719455 25.886386069771124 23.09941321146746 76-77 23.482311815936 26.192255045781504 26.284077089616133 24.041356048666366 78-79 23.45395400581335 26.460768060380325 26.672633622546645 23.412644311259687 80-81 23.906042940268634 26.353880929918034 26.220203849027378 23.519872280785947 82-83 23.260723741795804 26.13754420791148 27.283745940507124 23.317986109785593 84-85 23.09497191470797 26.97633200632601 26.536579047826798 23.392117031139225 86-87 23.052854595328593 27.015858559968585 26.319456184456648 23.611830660246167 88-89 23.01604397641939 26.866843721199103 25.229112018803406 24.8880002835781 90-91 23.345732104727805 25.896161198572145 26.08452889668206 24.673577800018 92-93 23.856320538375332 26.731368597862748 26.532245530126826 22.8800653336351 94-95 23.44577386827797 26.318433667264724 26.263422242339303 23.972370222118002 96-97 21.8605313817343 26.752049124452615 27.52568563186109 23.861733861952 98-99 22.694805814521033 27.114662551092472 25.535056457352272 24.655475177034223 100-101 21.98508642089783 26.501226889713557 26.497954274751024 25.015732414637586 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 359.0 1 266.0 2 172.5 3 178.0 4 179.0 5 189.5 6 243.5 7 440.5 8 681.5 9 823.5 10 830.0 11 801.0 12 842.0 13 913.5 14 977.5 15 1009.5 16 1059.0 17 1078.0 18 1068.5 19 1102.0 20 1151.0 21 1217.0 22 1364.5 23 1543.0 24 1654.0 25 1880.5 26 2324.0 27 2843.0 28 3259.0 29 3783.0 30 4694.5 31 5794.5 32 6758.5 33 7324.0 34 8195.0 35 9411.0 36 10927.5 37 12381.0 38 13857.0 39 15171.5 40 15709.0 41 17621.0 42 20178.0 43 22226.0 44 23895.5 45 24865.5 46 26370.0 47 36057.0 48 54019.5 49 53314.0 50 38022.0 51 31008.5 52 28663.0 53 28777.0 54 29126.5 55 26268.5 56 22231.5 57 18813.5 58 16659.0 59 15041.5 60 12679.5 61 10241.5 62 8000.0 63 6159.0 64 4643.0 65 3539.0 66 2519.5 67 1980.5 68 1587.0 69 1191.5 70 910.0 71 630.0 72 517.5 73 427.0 74 296.5 75 244.5 76 188.5 77 117.0 78 63.5 79 44.0 80 31.0 81 16.0 82 9.5 83 7.0 84 3.5 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018541645080192615 2 0.0 3 0.0 4 0.0 5 0.02344972760142007 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0024540412606137286 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010225171919223868 24-25 0.0 26-27 2.0450343838447738E-4 28-29 9.543493791275611E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 6.816781279482579E-5 40-41 4.7717468956378056E-4 42-43 0.012406541928658294 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 6.816781279482579E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0024540412606137286 84-85 5.453425023586063E-4 86-87 0.0 88-89 0.0 90-91 0.010634178795992825 92-93 0.0024540412606137286 94-95 0.0 96-97 0.0 98-99 0.0013633562558965158 100-101 0.017110121011501275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 733484.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.681229530521996 #Duplication Level Percentage of deduplicated Percentage of total 1 65.12058250143237 23.235824513949026 2 15.483387092371265 11.049325775056413 3 6.77981923830206 7.257368592519138 4 3.634027209151015 5.186662358795186 5 2.161716710096328 3.856635505645599 6 1.472087252940879 3.1515529896683465 7 0.9847450427303861 2.459583972909462 8 0.6875532223157917 1.962619547191983 9 0.5169959975496814 1.6602347569438232 >10 2.7886106946218794 18.32248546834801 >50 0.25133887733112664 6.228976126728344 >100 0.10722254504156298 6.568090059292434 >500 0.003458791775534277 0.8408603332828326 >1k 0.0076862039456317265 5.966104269482956 >5k 3.8431019728158637E-4 0.7578987381040796 >10k+ 3.8431019728158637E-4 1.4957769920823776 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10908 1.4871490039319195 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5527 0.7535270026340043 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4610 0.6285072339682938 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3452 0.47063057953547727 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3006 0.40982489052249266 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2874 0.39182858794465864 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2650 0.3612894078125767 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2617 0.3567903321681182 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2478 0.3378396802111566 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2474 0.337294337708798 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2382 0.3247514601545501 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2265 0.3088001919605608 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2079 0.28344176560088563 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1781 0.24281374917516949 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1699 0.23163422787681803 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1499 0.20436710275888773 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1474 0.20095871211914643 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1411 0.1923695677069984 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1370 0.18677980705782266 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1213 0.16537511384024736 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1112 0.15160521565569254 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1062 0.14478843437620997 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1000 0.1363356255896516 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 770 0.10497843170403172 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 745 0.10157004106429042 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.3633562558965158E-4 0.0 10-11 0.0 0.0 0.0 1.3633562558965158E-4 0.0 12-13 0.0 0.0 0.0 1.3633562558965158E-4 0.0 14-15 0.0 0.0 0.0 2.0450343838447735E-4 6.816781279482579E-5 16-17 0.0 0.0 0.0 2.7267125117930315E-4 1.3633562558965158E-4 18-19 0.0 0.0 0.0 2.7267125117930315E-4 1.3633562558965158E-4 20-21 0.0 0.0 0.0 2.7267125117930315E-4 1.3633562558965158E-4 22-23 0.0 0.0 0.0 6.816781279482579E-4 1.3633562558965158E-4 24-25 0.0 0.0 0.0 8.861815663327353E-4 1.3633562558965158E-4 26-27 0.0 0.0 0.0 0.0015678596942809932 1.3633562558965158E-4 28-29 0.0 0.0 0.0 0.004430907831663677 1.3633562558965158E-4 30-31 0.0 0.0 0.0 0.010702346608787648 1.3633562558965158E-4 32-33 0.0 0.0 0.0 0.021813700094344253 1.3633562558965158E-4 34-35 0.0 0.0 0.0 0.03203887201356812 1.3633562558965158E-4 36-37 0.0 0.0 0.0 0.03878748548025587 1.3633562558965158E-4 38-39 0.0 0.0 0.0 0.044649917380610894 1.3633562558965158E-4 40-41 0.0 0.0 0.0 0.05208020897524691 1.3633562558965158E-4 42-43 0.0 0.0 0.0 0.061214695889753555 1.3633562558965158E-4 44-45 0.0 0.0 0.0 0.0698038403019016 1.3633562558965158E-4 46-47 0.0 0.0 0.0 0.08261938910732886 1.3633562558965158E-4 48-49 0.0 0.0 0.0 0.09666195854306298 1.3633562558965158E-4 50-51 0.0 0.0 0.0 0.11288589798823151 1.3633562558965158E-4 52-53 0.0 0.0 0.0 0.14799232157756678 1.3633562558965158E-4 54-55 0.0 0.0 0.0 0.1982319996073534 1.3633562558965158E-4 56-57 0.0 0.0 0.0 0.26810400772204984 1.3633562558965158E-4 58-59 0.0 0.0 0.0 0.3524957599620442 1.3633562558965158E-4 60-61 0.0 0.0 0.0 0.4770383539381909 1.3633562558965158E-4 62-63 0.0 0.0 0.0 0.5637478118132093 1.3633562558965158E-4 64-65 0.0 0.0 0.0 0.6519569615697138 1.3633562558965158E-4 66-67 0.0 0.0 0.0 0.8105152941304786 1.3633562558965158E-4 68-69 0.0 0.0 0.0 1.0069067627923718 1.3633562558965158E-4 70-71 0.0 0.0 0.0 1.1711911916279019 1.3633562558965158E-4 72-73 0.0 0.0 0.0 1.3330897470156131 1.3633562558965158E-4 74-75 0.0 0.0 0.0 1.6310512567417965 1.3633562558965158E-4 76-77 0.0 0.0 0.0 1.8470068876758048 1.3633562558965158E-4 78-79 0.0 0.0 0.0 2.0626216795458387 1.3633562558965158E-4 80-81 0.0 0.0 0.0 2.2661026007383938 1.3633562558965158E-4 82-83 0.0 0.0 0.0 2.564064110464577 1.3633562558965158E-4 84-85 0.0 0.0 0.0 2.887793053427205 1.3633562558965158E-4 86-87 0.0 0.0 0.0 3.3106379961935093 1.3633562558965158E-4 88-89 0.0 0.0 0.0 3.8715909276821305 1.3633562558965158E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTAGT 65 1.3267464E-4 36.543 1 TAAGGTG 215 0.0 33.141388 5 GTAAGGT 205 0.0 32.44084 4 ACTCCGT 85 6.4079615E-4 27.93893 6 GGTAAGG 225 3.6379788E-12 27.44598 3 TAAGGCC 270 0.0 26.390366 4 ATAAGGC 300 0.0 25.334753 3 CCTATAA 435 0.0 25.118061 1 AGGTAAG 285 0.0 25.0014 2 AAGGTAA 270 1.8189894E-12 24.63269 1 CATAAGG 260 2.3646862E-11 23.75133 2 TACATGG 1945 0.0 22.713354 2 GAGTACT 2675 0.0 22.639912 12-13 GCATAAG 275 5.0931703E-11 22.457335 1 CTATAAG 445 0.0 22.416986 2 GTACATG 2005 0.0 22.035154 1 GCGGTCG 130 2.940346E-4 21.92281 9 AGTACTT 2695 0.0 21.855022 12-13 CATGGGG 1250 0.0 21.661213 4 GTCTAGA 110 0.0028743125 21.59359 1 >>END_MODULE