FastQCFastQC Report
Thu 26 May 2016
SRR937853_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937853_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693578
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT83131.1985674286093273No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68230.9837393919645663No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT47510.6849986591270196No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30790.4439298824357174No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25760.3714073975818149No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25100.36189152481768455No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24940.35958464657183475No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23540.3393994619206492No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22540.324981472884088No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG21990.3170515789139794No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21820.314600520777764No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA20440.2947036959073096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20200.29124337853853494No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA17920.25837036353517556No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC17300.24943121033250767No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT15420.2223253909437727No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG14600.2105026399337926No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA13620.19637301067796267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.18873147648858526No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC11720.16897883150849652No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC11470.16537433424935624No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9890.14259391157158965No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC9750.14057539310647107No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG7950.11462301284066105No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG7440.10726983843201486No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGA600.003935847431.6896881
CGGAACG700.00837141627.1547364
ATTCCGT908.9399616E-426.3908966
TACATGG19500.025.8355462
ACATGGG19650.025.6364773
ATCGGAC1501.0577223E-625.3370918
GTACATG20000.025.1933021
TATAAGA2151.6480044E-924.3164772
CATGGGG11850.024.0611574
GTAAGGT1201.7047928E-423.7603914
GCACGTC652.9404153E-421.92474470-71
GAGTACT23200.021.80661612-13
AGTACTT22900.021.36625512-13
TACATAG1353.7789534E-421.1234022
TAAGGTG1805.1410916E-621.1218765
ATAATGC1353.7807992E-421.1218743
GTACTTT25200.019.98167814-15
GTATAAG1707.9037534E-519.5730421
ATAAGGC2201.3366207E-619.4417273
CATGGGC4450.019.2218914
Produced by FastQC (version 0.11.5)