##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937853_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 693578 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87991112751558 33.0 31.0 34.0 30.0 34.0 2 32.01550798900772 33.0 31.0 34.0 30.0 34.0 3 32.04020312062955 34.0 31.0 34.0 30.0 34.0 4 35.61675687521807 37.0 35.0 37.0 33.0 37.0 5 35.591381214513724 37.0 35.0 37.0 33.0 37.0 6 35.65861662278792 37.0 35.0 37.0 33.0 37.0 7 35.616183039254416 37.0 35.0 37.0 33.0 37.0 8 35.60267338352716 37.0 35.0 37.0 33.0 37.0 9 37.24573876334024 39.0 37.0 39.0 34.0 39.0 10-11 37.14140947377224 39.0 37.0 39.0 34.0 39.0 12-13 37.03436095147193 39.0 37.0 39.0 33.0 39.0 14-15 38.21155154863621 40.0 38.0 41.0 33.5 41.0 16-17 38.146467304326265 40.0 38.0 41.0 33.0 41.0 18-19 38.07407962766985 40.0 38.0 41.0 33.0 41.0 20-21 37.984077493807476 40.0 38.0 41.0 33.0 41.0 22-23 37.792332657610245 40.0 37.5 41.0 33.0 41.0 24-25 37.59065815236354 40.0 37.0 41.0 32.0 41.0 26-27 37.54451265755257 40.0 37.0 41.0 32.0 41.0 28-29 37.61077773516461 40.0 37.0 41.0 33.0 41.0 30-31 37.47546634985538 40.0 37.0 41.0 32.0 41.0 32-33 37.2420535253425 39.5 36.5 41.0 32.0 41.0 34-35 37.267295098748804 40.0 37.0 41.0 32.0 41.0 36-37 37.19292566949932 40.0 37.0 41.0 31.5 41.0 38-39 37.06631626147312 40.0 36.0 41.0 31.0 41.0 40-41 36.89035551877366 39.5 36.0 41.0 31.0 41.0 42-43 36.637772536037765 39.0 36.0 41.0 30.0 41.0 44-45 36.42235696633976 39.0 36.0 41.0 30.0 41.0 46-47 36.10150120101849 39.0 35.0 41.0 29.5 41.0 48-49 35.75579242709544 39.0 35.0 40.0 28.0 41.0 50-51 34.783104423727394 38.0 34.0 39.5 26.5 40.5 52-53 34.89571684799691 38.0 34.5 39.5 27.0 40.5 54-55 35.25754493366283 38.0 35.0 40.0 27.0 41.0 56-57 34.99541507948638 38.0 34.5 40.0 26.0 41.0 58-59 34.73957939842383 38.0 34.0 40.0 26.0 41.0 60-61 34.34968381350043 38.0 34.0 40.0 25.5 41.0 62-63 33.98392754672149 37.0 33.5 40.0 25.0 41.0 64-65 33.59729619451598 36.5 33.0 39.5 23.0 41.0 66-67 33.115323006208385 36.0 32.5 39.0 22.0 40.5 68-69 32.585991049312405 35.5 32.0 39.0 21.5 40.0 70-71 32.05121558065567 35.0 31.5 37.5 19.5 40.0 72-73 31.592907358653243 35.0 31.0 37.0 18.5 39.0 74-75 31.13891444076946 35.0 31.0 36.5 16.5 39.0 76-77 30.66218219147666 34.5 30.5 36.0 12.0 38.5 78-79 30.133868144606662 34.0 30.0 35.5 7.5 37.0 80-81 29.593702510748614 34.0 29.0 35.0 4.5 37.0 82-83 29.116891683415563 34.0 29.0 35.0 2.0 36.0 84-85 28.671845848628415 34.0 29.0 35.0 2.0 36.0 86-87 28.31187840444766 34.0 28.0 35.0 2.0 35.5 88-89 27.81185749836356 33.0 27.0 35.0 2.0 35.0 90-91 27.308618497126496 33.0 26.0 35.0 2.0 35.0 92-93 26.750991957645716 33.0 25.0 35.0 2.0 35.0 94-95 26.12334661710723 32.0 24.5 34.5 2.0 35.0 96-97 25.418307674118843 32.0 21.5 34.0 2.0 35.0 98-99 24.490072493648874 31.5 10.0 34.0 2.0 35.0 100-101 22.073234012612858 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24574932554595108 1101 2 0.30190869094044004 1101 3 0.14975272255270156 1101 4 0.11261066832167188 1101 5 0.09447218474065266 1101 6 0.1002352533304105 1101 7 0.12168415075169747 1101 8 -0.0300371217888582 1101 9 0.18535167752256143 1101 10-11 0.17307236382203683 1101 12-13 0.40223400560342526 1101 14-15 0.45066550654417625 1101 16-17 0.44373216081234546 1101 18-19 0.5117276729214879 1101 20-21 0.4463334469165261 1101 22-23 0.36566848003027275 1101 24-25 0.5182285436733522 1101 26-27 0.4584367302276746 1101 28-29 0.3817150538261629 1101 30-31 0.6440481381786043 1101 32-33 0.41092328940543155 1101 34-35 0.5441753584781068 1101 36-37 0.5212949847196313 1101 38-39 0.6230133962573134 1101 40-41 0.6695233531364266 1101 42-43 0.6594209193717617 1101 44-45 0.9290574118889268 1101 46-47 0.8753220551813143 1101 48-49 0.826554676378187 1101 50-51 0.8776846645061838 1101 52-53 0.7694361318314051 1101 54-55 0.6583245453507658 1101 56-57 0.8314509144299578 1101 58-59 0.9512172240898309 1101 60-61 0.8458459291506912 1101 62-63 1.1527542232654895 1101 64-65 0.8733926386932467 1101 66-67 1.0545368802319857 1101 68-69 0.8273567739051018 1101 70-71 0.7455265157293276 1101 72-73 0.920673168865477 1101 74-75 0.9807959012426046 1101 76-77 1.153888548038264 1101 78-79 1.3266008076463365 1101 80-81 1.0535844163613355 1101 82-83 1.1593544257810606 1101 84-85 1.2192316613462673 1101 86-87 1.1019945373604472 1101 88-89 1.3642841627382651 1101 90-91 1.4820145482483404 1101 92-93 1.5470409007911705 1101 94-95 1.6879160864137184 1101 96-97 1.8533304084517646 1101 98-99 2.2781823988456473 1101 100-101 2.575271215736688 1102 1 0.2081590949761889 1102 2 0.2625629063724908 1102 3 0.3590841403114773 1102 4 0.1888616319491092 1102 5 0.14355586264380094 1102 6 0.10492899500817288 1102 7 0.2433342313459903 1102 8 0.35452731860640796 1102 9 0.2128290760788758 1102 10-11 0.405395932397262 1102 12-13 0.4142565720061171 1102 14-15 0.5231732155637303 1102 16-17 0.5035068471954105 1102 18-19 0.5894547385883158 1102 20-21 0.6353507221285213 1102 22-23 0.6086471400625086 1102 24-25 0.6194549786022208 1102 26-27 0.6351003016061867 1102 28-29 0.5411393302295551 1102 30-31 0.6323286764083278 1102 32-33 0.6567069813249873 1102 34-35 0.7546701373694091 1102 36-37 0.6214745698342128 1102 38-39 0.6979520745108658 1102 40-41 0.5224177395685601 1102 42-43 0.4881755623039936 1102 44-45 0.6870704120991746 1102 46-47 0.7452814076236649 1102 48-49 0.776203390373567 1102 50-51 0.7977167270193206 1102 52-53 0.7449328379775935 1102 54-55 0.7285449532279813 1102 56-57 0.8173372764735518 1102 58-59 0.8143786229264762 1102 60-61 0.8937942787196889 1102 62-63 0.8413273538282482 1102 64-65 0.8863998571388265 1102 66-67 1.1079927986565394 1102 68-69 1.1812699773531392 1102 70-71 1.158058928652455 1102 72-73 1.35784115540973 1102 74-75 1.064747124173433 1102 76-77 0.9232390631409331 1102 78-79 1.2248575179974495 1102 80-81 1.3323924968154621 1102 82-83 1.2398716309110611 1102 84-85 1.6331236649905314 1102 86-87 1.4880070469967208 1102 88-89 1.430961049696002 1102 90-91 1.9221233505470323 1102 92-93 2.049709272786405 1102 94-95 2.179762922211605 1102 96-97 2.27531337852675 1102 98-99 2.141155452318209 1102 100-101 2.6216593648711743 1103 1 0.1682983798776121 1103 2 0.1250988163211879 1103 3 0.19050854886140556 1103 4 0.0693171215341053 1103 5 0.07976473981609189 1103 6 0.17604279034252102 1103 7 0.2657108561423627 1103 8 0.20291251340275807 1103 9 0.3547555877017814 1103 10-11 0.1840750556120696 1103 12-13 0.2853642740740625 1103 14-15 0.43761121030559735 1103 16-17 0.5557619830125304 1103 18-19 0.36597344043583746 1103 20-21 0.2633191733456499 1103 22-23 0.32777533701121797 1103 24-25 0.23758818099006618 1103 26-27 0.3411889084525086 1103 28-29 0.3083442951663855 1103 30-31 0.07852961441292905 1103 32-33 0.06441818917167552 1103 34-35 0.2929994712499351 1103 36-37 0.3258400367845198 1103 38-39 0.35708579124208484 1103 40-41 0.43607077822674256 1103 42-43 0.40313417289588216 1103 44-45 0.4065588286205255 1103 46-47 0.41947803733049227 1103 48-49 0.37151731008223265 1103 50-51 0.18029410702229853 1103 52-53 0.32344636771721724 1103 54-55 0.4061941352069738 1103 56-57 0.36791138761831377 1103 58-59 0.1791941313647456 1103 60-61 0.326279094837453 1103 62-63 0.47943482112622604 1103 64-65 0.28816206562531477 1103 66-67 0.2843068384383969 1103 68-69 0.34925147532892 1103 70-71 0.5960276845329027 1103 72-73 0.601968769913853 1103 74-75 0.5556134970490767 1103 76-77 0.6207343722218752 1103 78-79 0.5747006163875739 1103 80-81 0.5956126447384662 1103 82-83 0.597811605068447 1103 84-85 0.6968986585836383 1103 86-87 0.7594226382317366 1103 88-89 0.9438061929146464 1103 90-91 1.0922088175690199 1103 92-93 1.3658037399213008 1103 94-95 1.157021597499643 1103 96-97 1.3003853776378484 1103 98-99 1.8131871803147988 1103 100-101 2.09766354413507 1104 1 0.2795825439631727 1104 2 0.39635249696204156 1104 3 0.35906475810008587 1104 4 0.30884333514155315 1104 5 0.1971593064751218 1104 6 0.32283350660551946 1104 7 0.16037449013727922 1104 8 0.29601436062898046 1104 9 0.3199778171073362 1104 10-11 0.48977542059058976 1104 12-13 0.3813077817468411 1104 14-15 0.5070443083521923 1104 16-17 0.41122813874844866 1104 18-19 0.5059426043548712 1104 20-21 0.615182562062472 1104 22-23 0.6355729081310955 1104 24-25 0.4639728170204833 1104 26-27 0.5069935753973667 1104 28-29 0.48694182161900557 1104 30-31 0.40120549664026584 1104 32-33 0.4804848844410543 1104 34-35 0.5564598907695313 1104 36-37 0.8154313901436723 1104 38-39 0.7378222524589546 1104 40-41 0.8051741613797461 1104 42-43 0.8702919834949512 1104 44-45 0.9783937713465249 1104 46-47 0.887093295509203 1104 48-49 1.2376967509970527 1104 50-51 0.9899146116447781 1104 52-53 1.0203104519110795 1104 54-55 0.9577250663038086 1104 56-57 0.9659726718803583 1104 58-59 1.111996355324031 1104 60-61 1.0840268071097796 1104 62-63 1.1512411611341733 1104 64-65 1.1696233902446096 1104 66-67 1.0046604150515606 1104 68-69 1.0800793281072032 1104 70-71 1.1060738994725305 1104 72-73 0.9438594095764188 1104 74-75 1.169855887452666 1104 76-77 1.189486686397263 1104 78-79 1.3914766982462012 1104 80-81 1.3070366878697968 1104 82-83 1.4590786271140885 1104 84-85 1.6077649487131538 1104 86-87 1.473879353111613 1104 88-89 1.6752864227551179 1104 90-91 1.3267638395989927 1104 92-93 1.6594116201660292 1104 94-95 1.8112411840924132 1104 96-97 2.0693861044603423 1104 98-99 2.3275551484051356 1104 100-101 2.874015757221404 1105 1 0.1439988209659191 1105 2 0.208006346583975 1105 3 0.17188437912776422 1105 4 0.1908943199285602 1105 5 0.08769916812832435 1105 6 0.053701330026576954 1105 7 0.15699310898799723 1105 8 0.31775825160036675 1105 9 0.33450165423425204 1105 10-11 0.3176888829960731 1105 12-13 0.31322608757827197 1105 14-15 0.36539342146421916 1105 16-17 0.4556208426940387 1105 18-19 0.5521387115800849 1105 20-21 0.45732358819893193 1105 22-23 0.2962013941740409 1105 24-25 0.275968025120342 1105 26-27 0.3323790528483812 1105 28-29 0.4746625775026132 1105 30-31 0.3798383881397953 1105 32-33 0.3038028888465192 1105 34-35 0.3613155540999031 1105 36-37 0.34991825750335437 1105 38-39 0.6099641802970552 1105 40-41 0.576921272714138 1105 42-43 0.48945507555869483 1105 44-45 0.5819509843155686 1105 46-47 0.23509538230443638 1105 48-49 0.3216000885812704 1105 50-51 0.23615966838757174 1105 52-53 0.3880920082481154 1105 54-55 0.5247311592305977 1105 56-57 0.5229215711602819 1105 58-59 0.5208182177243614 1105 60-61 0.4522463328147168 1105 62-63 0.6403337916554932 1105 64-65 0.7550725020685007 1105 66-67 0.600082991084335 1105 68-69 0.5440370254188451 1105 70-71 0.6887534991170021 1105 72-73 0.7580509052418165 1105 74-75 0.668010619454062 1105 76-77 1.0347707868210634 1105 78-79 0.950731377433641 1105 80-81 0.9009392525668893 1105 82-83 0.8140431387138349 1105 84-85 1.3302276247059943 1105 86-87 1.1755642470328596 1105 88-89 1.1633002316716592 1105 90-91 1.3638205566321346 1105 92-93 1.451469560937113 1105 94-95 1.3606318914129432 1105 96-97 1.4047160628532502 1105 98-99 2.0694978001808444 1105 100-101 2.7331989443502565 1106 1 0.010563145810898789 1106 2 -0.02148013827545725 1106 3 -0.0575870554637703 1106 4 -0.044326316998777315 1106 5 -0.10616333262419175 1106 6 -0.02952665143149602 1106 7 0.08517323059415816 1106 8 -0.26492792021348777 1106 9 -0.1896841262726383 1106 10-11 -0.33151947291266026 1106 12-13 0.009278402309476519 1106 14-15 -0.2496433011894439 1106 16-17 -0.2952671231117492 1106 18-19 -0.13091017169004004 1106 20-21 0.012610747310361603 1106 22-23 0.20354441987989702 1106 24-25 0.04026577962036981 1106 26-27 -0.24687565788546806 1106 28-29 -0.01744355698658495 1106 30-31 0.11573628712239525 1106 32-33 0.23185508317557435 1106 34-35 0.0804625049156229 1106 36-37 -0.016706268413201997 1106 38-39 -0.05735717302302845 1106 40-41 -0.1953531237701256 1106 42-43 -0.09362240322043647 1106 44-45 -0.1519979049746567 1106 46-47 -0.04845166483301 1106 48-49 0.2746884035532702 1106 50-51 0.15997521230424638 1106 52-53 -0.1184398080182163 1106 54-55 -0.0325275849750426 1106 56-57 0.050538812747184636 1106 58-59 0.17916208624522056 1106 60-61 -0.094236419578273 1106 62-63 0.04350789315449788 1106 64-65 0.23812590041899284 1106 66-67 0.10697601378014099 1106 68-69 0.016638012716420292 1106 70-71 0.13774814122163193 1106 72-73 0.10328147534596255 1106 74-75 0.4355667569533104 1106 76-77 0.5536513478950482 1106 78-79 0.45681921079707166 1106 80-81 0.7574198979223787 1106 82-83 0.5021489119965992 1106 84-85 0.5144429037379616 1106 86-87 0.9809642831534973 1106 88-89 1.2477472848292024 1106 90-91 1.052731519247267 1106 92-93 0.8765342770890605 1106 94-95 1.1026627745626634 1106 96-97 0.9994725495187176 1106 98-99 1.1700862656555273 1106 100-101 2.047124957233283 1107 1 0.09218503507293363 1107 2 0.13397064963464445 1107 3 0.30509915826222667 1107 4 0.1728871506667744 1107 5 0.058662724380894815 1107 6 0.05086816472281441 1107 7 -0.0627428978825435 1107 8 0.11771437721181144 1107 9 0.08203722049391615 1107 10-11 0.15440147774608448 1107 12-13 0.3993692487746472 1107 14-15 0.48002926403918167 1107 16-17 0.43037439932000865 1107 18-19 0.42755878551309223 1107 20-21 0.2858820811603593 1107 22-23 0.46755888642601207 1107 24-25 0.5560616549325132 1107 26-27 0.4835449913868999 1107 28-29 0.32337061678418877 1107 30-31 0.5830304832818669 1107 32-33 0.5492310842088983 1107 34-35 0.6481560380250713 1107 36-37 0.622835624293252 1107 38-39 0.4658764875297976 1107 40-41 0.4762272172270414 1107 42-43 0.5556984010506483 1107 44-45 0.6074634745138425 1107 46-47 0.4322757527162935 1107 48-49 0.2878471480637401 1107 50-51 0.2879944579772058 1107 52-53 0.221976105182506 1107 54-55 0.43902907566389615 1107 56-57 0.45254518491630336 1107 58-59 0.5175813146960948 1107 60-61 0.666640399503784 1107 62-63 0.6831089867564941 1107 64-65 0.7372917059817183 1107 66-67 0.7779212906250592 1107 68-69 0.6765073401942416 1107 70-71 0.8828183211923495 1107 72-73 0.9637611102378969 1107 74-75 0.9343935028415338 1107 76-77 1.085609067691827 1107 78-79 1.262451906913494 1107 80-81 1.1935744230235592 1107 82-83 1.377525644968273 1107 84-85 1.4362869578152875 1107 86-87 1.4321827452218727 1107 88-89 1.4397088795936916 1107 90-91 1.566100157841948 1107 92-93 1.5824672832028988 1107 94-95 1.968287505467682 1107 96-97 2.010042600323846 1107 98-99 2.5600694741287917 1107 100-101 2.7244800764961816 1108 1 0.05615796910607074 1108 2 0.03441831866829759 1108 3 0.10659910680601925 1108 4 0.12062307739312672 1108 5 0.013888102584040496 1108 6 0.12645476552552992 1108 7 -0.02717121938095346 1108 8 0.03087430311486372 1108 9 0.08221731723159564 1108 10-11 0.049291876017157676 1108 12-13 0.07273036846179082 1108 14-15 0.18702087667682576 1108 16-17 0.1905550106197893 1108 18-19 0.20280062970368817 1108 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760.0 9 962.0 10 1185.0 11 1570.0 12 2124.0 13 2603.0 14 3054.0 15 3820.0 16 4977.0 17 6191.0 18 5540.0 19 4163.0 20 4305.0 21 4754.0 22 4962.0 23 5637.0 24 6240.0 25 7148.0 26 8513.0 27 9971.0 28 11966.0 29 14621.0 30 18029.0 31 22725.0 32 29144.0 33 38221.0 34 52252.0 35 73997.0 36 106795.0 37 137750.0 38 87612.0 39 6787.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.275432958348645 17.967156779084938 14.730563518395847 23.026846744170573 2 15.53231548777631 20.79647041470603 37.74276021436649 25.928453883151175 3 20.933369769368575 22.35582101809801 30.86970791890958 25.841101293623836 4 13.435360381199112 15.654202905255366 36.494812870096226 34.415623843449296 5 13.864140043687756 36.811632334721644 34.33883598587304 14.98539163571756 6 33.03104784115851 33.928223090161126 16.40332282847899 16.63740624020137 7 27.754459828672072 32.384850669606415 20.949311032865545 18.91137846885597 8 25.704761120335007 31.536046170541425 20.57010908281711 22.18908362630646 9 24.61847917657106 17.556677210479847 21.97554162879516 35.84930198415394 10-11 25.505684708221587 24.853691736798297 27.02172731896791 22.618896236012205 12-13 26.756023920116 22.923300004985165 27.226363279717535 23.0943127951813 14-15 23.392430120577675 24.698917040536926 26.942138610141818 24.96651422874358 16-17 22.15556229861141 27.61100755703903 27.283222795381967 22.95020734896759 18-19 21.76310072456491 27.42690231703273 28.69844907419114 22.11154788421122 20-21 22.937967461703717 25.369960226476486 28.14818346386504 23.543888847954765 22-23 22.41316046083282 25.083101592690237 28.9732384701506 23.530499476326337 24-25 24.024220326091747 25.236031166009486 28.02275549186432 22.716993016034444 26-27 22.87473857967947 24.725901471177846 29.795956699042453 22.603403250100236 28-29 21.926565600077456 27.12887940938038 28.495388132406767 22.449166858135396 30-31 22.262561194823512 25.44252455017831 28.273236693196463 24.021677561801713 32-33 22.548726352112922 25.73797592004948 28.67116648459311 23.042131243244494 34-35 24.09034457197807 25.179120641734876 27.66184797160368 23.068686814683378 36-37 22.886627279116823 24.71223226855962 29.410205271305284 22.990935181018273 38-39 22.6684445895548 25.842773733067414 28.73315048465494 22.755631192722845 40-41 22.69040189535119 26.117274854814944 28.43749097107856 22.75483227875531 42-43 22.43321341366506 26.013899122997124 26.998540736566827 24.55434672677098 44-45 22.984141569343883 25.22619555865698 27.49808388432946 24.29157898766968 46-47 23.744006911781597 26.27582256196801 27.447820311015725 22.53235021523467 48-49 23.410617604688497 25.809266887932992 27.38828193972636 23.391833567652153 50-51 21.798866947629744 26.53395941906681 28.690458213545906 22.976715419757536 52-53 23.24602827628817 26.810733861089936 26.25539710010876 23.687840762513144 54-55 22.30452199229177 25.502389406423042 27.47654841844051 24.716540182844675 56-57 23.54760291829946 25.762399233881396 26.878427781332647 23.8115700664865 58-59 24.648475245579853 25.206581727790205 26.89881142385039 23.246131602779556 60-61 23.481153585870732 25.333258220682414 28.543890705315018 22.64169748813184 62-63 22.33303405230604 26.0812219333747 28.375814144315093 23.209929870004167 64-65 22.34420488465091 26.954340640015367 27.362105063977705 23.339349411356014 66-67 22.848824359598474 25.889584997565944 26.533922899022187 24.727667743813388 68-69 22.18132439616318 25.604342424592623 27.89588870911823 24.318444470125968 70-71 23.72863973190426 25.60408210432044 26.795923673604992 23.871354490170305 72-73 23.129414823695956 24.82911291837 28.62062490068904 23.420847357245005 74-75 22.30957858280456 27.31602497661602 26.817792769204658 23.556603671374763 76-77 22.928264604190357 25.64501750436706 26.936280636254484 24.490437255188098 78-79 23.00756192695845 25.76112344500788 27.50404393676793 23.727270691265748 80-81 23.57300848198355 25.644239229210037 26.88077980504874 23.90197248375767 82-83 22.86992632787167 25.349525454410177 28.116040644997714 23.664507572720435 84-85 22.789839658326837 26.11361224892216 27.244360883247946 23.852187209503057 86-87 22.718463713198727 26.359170459445735 27.321083276932224 23.601282550423317 88-89 22.58048903468059 25.838842058802918 26.06985817575284 25.510810730763644 90-91 22.776864757873142 24.982680714732048 27.126443424270114 25.114011103124696 92-93 23.61291496975537 25.491520379549513 27.571588106716515 23.323976543978596 94-95 22.88246210033524 25.167818928198134 27.492037946256048 24.457681025210583 96-97 21.45301478756152 25.501140449332706 28.587576252652504 24.458268510453276 98-99 22.287125887991216 25.93450805196306 26.826273344192074 24.952092715853645 100-101 21.94995648547048 24.526883312448767 28.11486391327428 25.408296288806476 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1068.0 1 656.0 2 226.5 3 208.0 4 181.5 5 183.0 6 258.5 7 480.5 8 752.0 9 869.0 10 802.5 11 711.5 12 733.0 13 817.0 14 865.5 15 876.5 16 890.0 17 902.0 18 908.5 19 933.0 20 999.0 21 1101.5 22 1281.0 23 1486.5 24 1653.0 25 1838.5 26 2163.5 27 2733.0 28 3293.5 29 3694.0 30 4377.0 31 5511.5 32 6647.5 33 7168.5 34 7961.0 35 9203.5 36 10513.5 37 11809.5 38 13326.0 39 14517.0 40 14942.5 41 16823.0 42 19183.5 43 21084.0 44 22757.0 45 23684.0 46 25647.5 47 34518.0 48 50140.0 49 49874.5 50 36225.5 51 29194.0 52 26588.5 53 27129.0 54 27583.0 55 24622.5 56 20910.5 57 17722.5 58 15475.0 59 13729.0 60 11609.5 61 9471.5 62 7340.0 63 5536.5 64 4196.5 65 3278.5 66 2463.0 67 1893.5 68 1449.5 69 1090.5 70 846.5 71 621.5 72 475.0 73 391.5 74 301.0 75 244.5 76 175.5 77 106.5 78 64.5 79 39.0 80 23.0 81 15.0 82 11.0 83 9.5 84 4.5 85 4.0 86 4.5 87 2.0 88 1.0 89 1.0 90 0.5 91 1.5 92 2.0 93 0.5 94 0.5 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31315872187410787 2 0.29585713503023453 3 0.2818716856647702 4 0.26903967542223084 5 0.2668769770667467 6 0.21872089368463243 7 0.20978174048196455 8 0.22131613171121348 9 0.22275793061486956 10-11 0.22290211050523517 12-13 0.21980224286237454 14-15 0.21526057631585777 16-17 0.21742327467134193 18-19 0.21735118472615916 20-21 0.20314946552514643 22-23 0.1950753916646722 24-25 0.19457076204839255 26-27 0.20841203152349125 28-29 0.22477644907998814 30-31 0.22001851269792294 32-33 0.22347883006669764 34-35 0.21864880373944962 36-37 0.20134721689557628 38-39 0.19961705821118894 40-41 0.19579629111650027 42-43 0.20877248124940526 44-45 0.20509589404508216 46-47 0.20567261360654462 48-49 0.21641401543878264 50-51 0.22398345968297728 52-53 0.2386898085002696 54-55 0.2105747298789754 56-57 0.1812341221895735 58-59 0.17835052438226126 60-61 0.1851990691746278 62-63 0.19658928051351107 64-65 0.1826038311480468 66-67 0.18988491561151016 68-69 0.192912693309188 70-71 0.18570369879090745 72-73 0.18858729659821966 74-75 0.19183134413144592 76-77 0.21122353938562063 78-79 0.21518848637067495 80-81 0.20257274596368396 82-83 0.20033795766301699 84-85 0.19284060336400521 86-87 0.19658928051351107 88-89 0.2045191744836197 90-91 0.20624933316800703 92-93 0.20026586771783422 94-95 0.2001937777726514 96-97 0.18844311670785405 98-99 0.1954358413905862 100-101 0.18462234961316534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 693578.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.21654980510495 #Duplication Level Percentage of deduplicated Percentage of total 1 67.37435004666915 25.748152041451068 2 14.956092288684076 11.43140491680482 3 6.437866881643818 7.3809918096293 4 3.3833716876368594 5.172031704390241 5 1.9550899091245946 3.7358395442759083 6 1.1878565548463524 2.72374675137636 7 0.8557670521383158 2.289312491872825 8 0.6099251484283931 1.864738784983977 9 0.4583463998407448 1.5764776215753933 >10 2.4650393064729035 17.64808989279092 >50 0.21401978529918408 5.677451933205963 >100 0.09053226100933634 5.757795177561017 >500 0.0037879607116877018 1.0156557290131247 >1k 0.007197125352206633 5.7871820237681995 >5k 7.575921423375404E-4 2.191129577300835 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8313 1.1985674286093273 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6823 0.9837393919645663 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4751 0.6849986591270196 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3079 0.4439298824357174 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2576 0.3714073975818149 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2510 0.36189152481768455 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2494 0.35958464657183475 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2354 0.3393994619206492 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2254 0.324981472884088 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2199 0.3170515789139794 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2182 0.314600520777764 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2044 0.2947036959073096 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2020 0.29124337853853494 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1792 0.25837036353517556 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1730 0.24943121033250767 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1542 0.2223253909437727 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1460 0.2105026399337926 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1362 0.19637301067796267 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1309 0.18873147648858526 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1172 0.16897883150849652 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1147 0.16537433424935624 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 989 0.14259391157158965 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 975 0.14057539310647107 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 795 0.11462301284066105 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 744 0.10726983843201486 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.4417989036561137E-4 0.0 10-11 0.0 0.0 0.0 1.4417989036561137E-4 0.0 12-13 0.0 0.0 0.0 1.4417989036561137E-4 0.0 14-15 0.0 0.0 0.0 1.4417989036561137E-4 0.0 16-17 0.0 0.0 0.0 1.4417989036561137E-4 0.0 18-19 0.0 0.0 0.0 1.4417989036561137E-4 0.0 20-21 0.0 0.0 0.0 3.604497259140284E-4 0.0 22-23 0.0 0.0 0.0 0.0010092592325592795 0.0 24-25 0.0 0.0 0.0 0.001369708958473308 0.0 26-27 0.0 0.0 0.0 0.001802248629570142 0.0 28-29 0.0 0.0 0.0 0.004253306765785535 0.0 30-31 0.0 0.0 0.0 0.012399470571442577 0.0 32-33 0.0 0.0 0.0 0.022492062897035373 0.0 34-35 0.0 0.0 0.0 0.032296295441896945 0.0 36-37 0.0 0.0 0.0 0.04015409946682277 0.0 38-39 0.0 0.0 0.0 0.04599338502663003 0.0 40-41 0.0 0.0 0.0 0.053346559435276206 0.0 42-43 0.0 0.0 0.0 0.06279034225422375 0.0 44-45 0.0 0.0 0.0 0.07129695578579481 0.0 46-47 0.0 0.0 0.0 0.08470568558979669 0.0 48-49 0.0 0.0 0.0 0.09782605561306731 0.0 50-51 0.0 0.0 0.0 0.11411838322438139 0.0 52-53 0.0 0.0 0.0 0.15081216532242947 0.0 54-55 0.0 0.0 0.0 0.2004100476081998 0.0 56-57 0.0 0.0 0.0 0.27033729443552135 0.0 58-59 0.0 0.0 0.0 0.363189143830975 0.0 60-61 0.0 0.0 0.0 0.4904278970786271 0.0 62-63 0.0 0.0 0.0 0.5750614927232409 0.0 64-65 0.0 0.0 0.0 0.6603438978745 0.0 66-67 0.0 0.0 0.0 0.8216811951936192 0.0 68-69 0.0 0.0 0.0 1.0223796025825502 0.0 70-71 0.0 0.0 0.0 1.1962605503634776 0.0 72-73 0.0 0.0 0.0 1.363725493023135 0.0 74-75 0.0 0.0 0.0 1.662033686189585 0.0 76-77 0.0 0.0 0.0 1.8921447912131009 0.0 78-79 0.0 0.0 0.0 2.104305499886098 0.0 80-81 0.0 0.0 0.0 2.3101943833281906 0.0 82-83 0.0 0.0 0.0 2.618883528600965 0.0 84-85 0.0 0.0 0.0 2.934421218666105 0.0 86-87 0.0 0.0 0.0 3.368979408228058 0.0 88-89 0.0 0.0 0.0 3.9313530705991253 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGGA 60 0.0039358474 31.689688 1 CGGAACG 70 0.008371416 27.154736 4 ATTCCGT 90 8.9399616E-4 26.390896 6 TACATGG 1950 0.0 25.835546 2 ACATGGG 1965 0.0 25.636477 3 ATCGGAC 150 1.0577223E-6 25.337091 8 GTACATG 2000 0.0 25.193302 1 TATAAGA 215 1.6480044E-9 24.316477 2 CATGGGG 1185 0.0 24.061157 4 GTAAGGT 120 1.7047928E-4 23.760391 4 GCACGTC 65 2.9404153E-4 21.924744 70-71 GAGTACT 2320 0.0 21.806616 12-13 AGTACTT 2290 0.0 21.366255 12-13 TACATAG 135 3.7789534E-4 21.123402 2 TAAGGTG 180 5.1410916E-6 21.121876 5 ATAATGC 135 3.7807992E-4 21.121874 3 GTACTTT 2520 0.0 19.981678 14-15 GTATAAG 170 7.9037534E-5 19.573042 1 ATAAGGC 220 1.3366207E-6 19.441727 3 CATGGGC 445 0.0 19.221891 4 >>END_MODULE