FastQCFastQC Report
Thu 26 May 2016
SRR937853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937853_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693578
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT108701.5672354082741955No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52190.7524748478181257No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT43530.6276150627615062No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33340.4806957544789483No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28470.41048014787089554No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27730.3998108359838403No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA26050.37558861440241764No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24470.35280819172465105No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24090.3473293558907578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23670.34127380049540207No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22140.31921427726946355No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21340.3076798860402147No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC18470.2663002575052842No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA17410.2510171891265294No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC16450.23717591965143067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14410.20776322201684597No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT13280.1914708944055319No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA12700.18310846076432644No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC11830.17056481030251824No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG10890.15701190060815076No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG9770.14086375288720232No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC9550.13769179529915884No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT9280.13379893825928735No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG7460.10755819821274609No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC7180.10352116128250896No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG352.7859167E-454.290664
CTAGGCG500.001614651438.003463
GCGTCAG804.483744E-429.6966271
TATATAG1257.3801493E-626.602422
GAGTACT26850.022.73156712-13
GTTCTAG1302.933774E-421.929821
GTGTAAG1754.034644E-621.7209631
AGTACTT27050.021.59774212-13
ATCTAGT1100.002871101321.5975481
AAGTGCG1100.002877114821.5897629
GTACTTT28900.021.28347214-15
TGCTAGC1656.313665E-520.1533492
GTACATG19050.019.953641
TACTTTT29550.019.85089114-15
TAAGGGT1200.004759120319.793474
GCATAAG2651.845001E-819.7230421
AGAGTAC40550.019.5612310-11
TACATGG18800.019.2038762
GTCTAGG1507.7008957E-419.0058421
TGGACCG1507.71211E-419.0017325
Produced by FastQC (version 0.11.5)