##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937853_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 693578 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25712176568461 33.0 31.0 34.0 31.0 34.0 2 32.46854715691674 34.0 31.0 34.0 31.0 34.0 3 32.53318300176765 34.0 31.0 34.0 31.0 34.0 4 36.11393960016033 37.0 35.0 37.0 35.0 37.0 5 36.02718223473063 37.0 35.0 37.0 35.0 37.0 6 36.08922284155495 37.0 35.0 37.0 35.0 37.0 7 36.04339237980444 37.0 35.0 37.0 35.0 37.0 8 36.01219329332822 37.0 35.0 37.0 35.0 37.0 9 37.700617666650324 39.0 37.0 39.0 35.0 39.0 10-11 37.619254070919204 39.0 37.0 39.0 35.0 39.0 12-13 37.53305828616248 39.0 37.0 39.0 35.0 39.0 14-15 38.73234445152528 40.0 38.0 41.0 35.0 41.0 16-17 38.71280591368239 40.0 38.0 41.0 34.5 41.0 18-19 38.6782870852305 40.0 38.0 41.0 34.5 41.0 20-21 38.5539088610077 40.0 38.0 41.0 34.0 41.0 22-23 38.50989074047908 40.0 38.0 41.0 34.0 41.0 24-25 38.49891865082226 40.0 38.0 41.0 34.0 41.0 26-27 38.30208065999787 40.0 38.0 41.0 34.0 41.0 28-29 38.2832435573216 40.0 38.0 41.0 34.0 41.0 30-31 38.36074241109147 40.0 38.0 41.0 34.0 41.0 32-33 38.24687778447414 40.0 38.0 41.0 34.0 41.0 34-35 38.071482947844366 40.0 38.0 41.0 33.5 41.0 36-37 37.89584805169714 40.0 37.0 41.0 33.0 41.0 38-39 37.74330140229361 40.0 37.0 41.0 33.0 41.0 40-41 37.61989927592859 40.0 37.0 41.0 33.0 41.0 42-43 37.39377834937095 40.0 37.0 41.0 32.5 41.0 44-45 37.115354725784265 40.0 36.0 41.0 31.5 41.0 46-47 36.78704413923164 39.5 36.0 41.0 31.0 41.0 48-49 36.45057008728651 39.0 36.0 41.0 30.0 41.0 50-51 36.1832360599673 39.0 35.0 41.0 30.0 41.0 52-53 35.95986031852222 39.0 35.0 40.0 29.0 41.0 54-55 35.739149742350534 39.0 35.0 40.0 28.5 41.0 56-57 35.396378633693686 38.0 35.0 40.0 28.0 41.0 58-59 35.12786665667019 38.0 34.0 40.0 28.0 41.0 60-61 34.68508444616178 38.0 34.0 40.0 26.5 41.0 62-63 34.29386673164374 37.0 34.0 40.0 26.0 41.0 64-65 33.88672002283809 36.5 33.0 39.0 26.0 40.5 66-67 33.39344745652255 36.0 33.0 39.0 25.0 40.0 68-69 32.905848368892904 35.5 32.0 38.5 24.0 40.0 70-71 32.40062689416331 35.0 32.0 37.5 22.5 39.5 72-73 31.910112489150464 35.0 31.5 37.0 21.5 39.0 74-75 31.44138727006912 34.5 31.0 36.5 20.5 39.0 76-77 30.19304461790887 33.5 29.5 35.0 20.0 37.0 78-79 30.806088140050576 34.0 31.0 35.0 20.0 37.0 80-81 30.627713825986408 34.0 31.0 35.0 18.5 37.0 82-83 30.278636288924965 34.0 30.5 35.0 17.5 36.0 84-85 29.90732765456805 34.0 30.0 35.0 14.5 36.0 86-87 29.68698257441845 34.0 30.0 35.0 9.0 35.5 88-89 29.315469204617216 34.0 29.5 35.0 6.0 35.0 90-91 28.847778476249246 34.0 29.0 35.0 2.0 35.0 92-93 28.442847091459072 33.5 29.0 35.0 2.0 35.0 94-95 27.97768311566976 33.0 28.0 35.0 2.0 35.0 96-97 27.45851295744675 33.0 27.0 35.0 2.0 35.0 98-99 26.729906369579197 33.0 25.5 34.5 2.0 35.0 100-101 24.33992067222432 30.5 20.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2816543055267857 1101 2 0.2642945398750811 1101 3 0.25276873262696853 1101 4 0.16392076631255037 1101 5 0.1475677038038441 1101 6 0.23080259044287743 1101 7 0.13491758423830902 1101 8 0.16392097019985385 1101 9 0.23586921378423398 1101 10-11 0.27714728139601874 1101 12-13 0.2017019316146218 1101 14-15 0.39127085006937534 1101 16-17 0.3512712162359293 1101 18-19 0.25938244484300554 1101 20-21 0.32549404504938906 1101 22-23 0.3325560010254307 1101 24-25 0.1765124611473894 1101 26-27 0.27337371458270354 1101 28-29 0.2772009922391163 1101 30-31 0.33723629871026617 1101 32-33 0.2165075256583222 1101 34-35 0.2748763828342575 1101 36-37 0.4373939117416157 1101 38-39 0.5510312114308036 1101 40-41 0.4035072661296084 1101 42-43 0.6352199468655613 1101 44-45 0.6102171312334903 1101 46-47 0.515445808665234 1101 48-49 0.49509964105369875 1101 50-51 0.6540333049550426 1101 52-53 0.5783576889246973 1101 54-55 0.8095717523611441 1101 56-57 0.8410940487352576 1101 58-59 0.5468777976357586 1101 60-61 0.4606194811630573 1101 62-63 0.4192624784482888 1101 64-65 0.6958942704022917 1101 66-67 0.7563047509009166 1101 68-69 0.5376633355902456 1101 70-71 0.3633324780841676 1101 72-73 0.5789331166143832 1101 74-75 0.7268060701779895 1101 76-77 0.5143419559179385 1101 78-79 0.672981979800312 1101 80-81 0.8087292508826742 1101 82-83 0.8016086469254127 1101 84-85 0.8185072409429495 1101 86-87 0.8317871090073297 1101 88-89 0.8220433582157121 1101 90-91 0.7611514463529083 1101 92-93 0.9184521285442706 1101 94-95 1.0141761037625159 1101 96-97 1.126209953188404 1101 98-99 1.2744712946360721 1101 100-101 1.6755295964394818 1102 1 0.25185193675532247 1102 2 0.2420732910139165 1102 3 0.259219780352808 1102 4 0.18568473015023557 1102 5 0.16266511777818948 1102 6 0.19051945917929913 1102 7 0.18870902021840408 1102 8 0.24169621723888923 1102 9 0.3012644762413075 1102 10-11 0.3799321647094942 1102 12-13 0.3025946010378462 1102 14-15 0.3363309453808938 1102 16-17 0.3494079111690027 1102 18-19 0.49789600570922943 1102 20-21 0.32024928367857797 1102 22-23 0.32574315499557827 1102 24-25 0.3602096368429457 1102 26-27 0.3113432639554716 1102 28-29 0.3768987636316439 1102 30-31 0.4111587880227603 1102 32-33 0.3074625680579359 1102 34-35 0.27587943140108706 1102 36-37 0.10982891069038914 1102 38-39 0.3047972426873713 1102 40-41 0.43600428764478494 1102 42-43 0.48530930141037487 1102 44-45 0.4882508056325392 1102 46-47 0.3562002413514733 1102 48-49 0.2511398330783692 1102 50-51 0.25946465964002385 1102 52-53 0.45000776951899724 1102 54-55 0.3943578270685535 1102 56-57 0.477552455070672 1102 58-59 0.4235282286400306 1102 60-61 0.4828310937497875 1102 62-63 0.5573792000125195 1102 64-65 0.7398120291800581 1102 66-67 0.6079271491068141 1102 68-69 0.5340497326748377 1102 70-71 0.5086667692047868 1102 72-73 0.6405071582430857 1102 74-75 0.6136245227675161 1102 76-77 0.5792308759543836 1102 78-79 0.6657127247719288 1102 80-81 0.6625522237609438 1102 82-83 0.7064653292437235 1102 84-85 0.7270879053202712 1102 86-87 1.0167273641057974 1102 88-89 0.881309950128248 1102 90-91 1.1063166646583262 1102 92-93 1.0383615475645236 1102 94-95 1.304935792598453 1102 96-97 1.40933946471689 1102 98-99 1.34286081036948 1102 100-101 2.0202296454968796 1103 1 0.23013840271457298 1103 2 0.2345803654754235 1103 3 0.1423097115885028 1103 4 0.023179107319840853 1103 5 0.06752413930713175 1103 6 0.08435504493037627 1103 7 0.2205696349573003 1103 8 0.12174309954744444 1103 9 0.23361129779641487 1103 10-11 0.25497808822466794 1103 12-13 0.28089988443745995 1103 14-15 0.17175271590489416 1103 16-17 0.20011392361001157 1103 18-19 0.24033094664934396 1103 20-21 0.35750243918411684 1103 22-23 0.3829553422452392 1103 24-25 0.09340521456524442 1103 26-27 0.23161466520961227 1103 28-29 -0.056978543072922605 1103 30-31 -0.05460332727259498 1103 32-33 0.2618526768944207 1103 34-35 0.1466134728675499 1103 36-37 0.1523599101832147 1103 38-39 0.2830980457440404 1103 40-41 0.2330107017037406 1103 42-43 0.41024473966856334 1103 44-45 0.6252247461658413 1103 46-47 0.31773560111405175 1103 48-49 0.31233683734186 1103 50-51 0.5673789166289112 1103 52-53 0.48180565945667553 1103 54-55 0.6223373895361775 1103 56-57 0.628168143797943 1103 58-59 0.1654954217768605 1103 60-61 0.25710155950832103 1103 62-63 0.3564295465222429 1103 64-65 0.3627400945859023 1103 66-67 0.41993016706582154 1103 68-69 0.44978613080169083 1103 70-71 0.28070692024610366 1103 72-73 0.11267257967310584 1103 74-75 0.2629973643623025 1103 76-77 0.2725200390800886 1103 78-79 0.5029795005200093 1103 80-81 0.4414132488992486 1103 82-83 0.5406254351948725 1103 84-85 0.42152045196512233 1103 86-87 0.5065489210070453 1103 88-89 0.6591387042524026 1103 90-91 0.3135253572404899 1103 92-93 0.5743342210568407 1103 94-95 0.5293740920036427 1103 96-97 0.6783580724772023 1103 98-99 0.5467352317331908 1103 100-101 1.2391284488868841 1104 1 0.22555609055704906 1104 2 0.14376477660227494 1104 3 0.2535233484241033 1104 4 0.22508295125087585 1104 5 0.23469135353953163 1104 6 0.2326718925190292 1104 7 0.2634972211033997 1104 8 0.2533391116232764 1104 9 0.22829432678507544 1104 10-11 0.3009990779178935 1104 12-13 0.26627233688544294 1104 14-15 0.44222178088988073 1104 16-17 0.4390680828566289 1104 18-19 0.35383965774425263 1104 20-21 0.4358719275285097 1104 22-23 0.2807202161483886 1104 24-25 0.16678098179398404 1104 26-27 0.31948476185153396 1104 28-29 0.39805347825765836 1104 30-31 0.5101544018283235 1104 32-33 0.44777524189213835 1104 34-35 0.5169902647005031 1104 36-37 0.3955701930562583 1104 38-39 0.22185688369005163 1104 40-41 0.451879287887067 1104 42-43 0.311097139848016 1104 44-45 0.011792439591722825 1104 46-47 0.2599355811313302 1104 48-49 0.14743531101462537 1104 50-51 0.2176048976923468 1104 52-53 0.22512663093036878 1104 54-55 0.32610530786562464 1104 56-57 0.2894296803908176 1104 58-59 0.1917276405837427 1104 60-61 0.23503436663000343 1104 62-63 0.4402835080966483 1104 64-65 0.1978679546128177 1104 66-67 0.13565049517426075 1104 68-69 0.3086307377200228 1104 70-71 0.6127521517224181 1104 72-73 0.5758073983886867 1104 74-75 0.5588533006997061 1104 76-77 0.5179656439590055 1104 78-79 0.7744663129038116 1104 80-81 0.6080397542152411 1104 82-83 0.5190529126114605 1104 84-85 0.5563558198137066 1104 86-87 0.8347758855338014 1104 88-89 0.7298021109928499 1104 90-91 0.944232921875777 1104 92-93 1.2207801374311167 1104 94-95 1.1543699289368057 1104 96-97 1.3177042456882084 1104 98-99 1.114810680217932 1104 100-101 1.6849086663850876 1105 1 0.3378179447132439 1105 2 0.22374571205331506 1105 3 0.24592973247170846 1105 4 0.17183102869416444 1105 5 0.20979794755481151 1105 6 0.18660137945079924 1105 7 0.3056131284084671 1105 8 0.22563110837715072 1105 9 0.3522525393547937 1105 10-11 0.2426010152882725 1105 12-13 0.3265253286026635 1105 14-15 0.41301630884906615 1105 16-17 0.5642757962700884 1105 18-19 0.502031101883837 1105 20-21 0.5539465368984793 1105 22-23 0.39121731138096294 1105 24-25 0.431337022550899 1105 26-27 0.2574348075806867 1105 28-29 0.38619610169416774 1105 30-31 0.32073265020847685 1105 32-33 0.26840746355616574 1105 34-35 0.5080408759253956 1105 36-37 0.5203818018207969 1105 38-39 0.40123110275202833 1105 40-41 0.49736691525241383 1105 42-43 0.6584422725913797 1105 44-45 0.5870394414337738 1105 46-47 0.5993264172663828 1105 48-49 0.5753957907198952 1105 50-51 0.7091256819151397 1105 52-53 0.8127816914087802 1105 54-55 0.7801217445983752 1105 56-57 0.8108386536102756 1105 58-59 0.8239815082397044 1105 60-61 0.578914388786103 1105 62-63 0.6444352000753639 1105 64-65 0.7730891935508737 1105 66-67 0.7897545257805874 1105 68-69 0.6732692197697219 1105 70-71 0.7482843489116888 1105 72-73 0.7486893656440294 1105 74-75 0.8449728921000492 1105 76-77 0.3266148949025691 1105 78-79 0.5111243489976403 1105 80-81 0.7664687323296526 1105 82-83 0.6975254300336253 1105 84-85 0.8395191179806751 1105 86-87 0.8486653111498548 1105 88-89 0.8414231129121852 1105 90-91 0.9337119959570082 1105 92-93 0.6540735382632583 1105 94-95 0.9731343400612253 1105 96-97 1.0387274194203506 1105 98-99 1.2902141917017467 1105 100-101 1.422304165605759 1106 1 -0.40327229669305 1106 2 -0.28055866456458745 1106 3 -0.29177118860468454 1106 4 -0.24796609369461464 1106 5 -0.2154022210081905 1106 6 -0.2751408395606987 1106 7 -0.1427945532482653 1106 8 -0.27667158261074576 1106 9 -0.6246302491588409 1106 10-11 -0.5698049205373863 1106 12-13 -0.3240902273542261 1106 14-15 -0.47157823693384415 1106 16-17 -0.36484496707949887 1106 18-19 -0.30226273954654204 1106 20-21 -0.4313042005646466 1106 22-23 -0.4121584637078328 1106 24-25 -0.5771663788096504 1106 26-27 -0.467536596910314 1106 28-29 -0.568668144889422 1106 30-31 -0.8082255702478491 1106 32-33 -0.8102602867297932 1106 34-35 -1.2648882072122873 1106 36-37 -1.2926186195866975 1106 38-39 -1.139923854806213 1106 40-41 -1.4056943092373757 1106 42-43 -1.4006375540295366 1106 44-45 -1.629037254731415 1106 46-47 -1.619015344216976 1106 48-49 -1.8275149310909455 1106 50-51 -2.050656992216453 1106 52-53 -1.9105341836137981 1106 54-55 -2.029244037112541 1106 56-57 -1.8837537851351343 1106 58-59 -2.116351704691489 1106 60-61 -2.280854740104388 1106 62-63 -2.015391119833133 1106 64-65 -2.4458395283803824 1106 66-67 -2.1622953207066757 1106 68-69 -2.0272981753299106 1106 70-71 -2.0797663403905844 1106 72-73 -2.1421284659133697 1106 74-75 -2.474514194770098 1106 76-77 -2.5731804214540617 1106 78-79 -2.4236703653482756 1106 80-81 -2.7787573226941262 1106 82-83 -2.3822259395407563 1106 84-85 -2.079134184048101 1106 86-87 -2.205891909968681 1106 88-89 -2.5678689224860456 1106 90-91 -2.881387937821817 1106 92-93 -2.9271753329666517 1106 94-95 -2.728546774862618 1106 96-97 -3.134862370477908 1106 98-99 -3.251414849295184 1106 100-101 -1.9503028417989725 1107 1 0.09128122091652813 1107 2 0.07405469976848877 1107 3 0.034963935824833925 1107 4 0.014153944193957102 1107 5 0.12863032872056124 1107 6 0.07902044520636764 1107 7 -0.012434181251364862 1107 8 0.12601290890737715 1107 9 0.04816101924726013 1107 10-11 0.12823822076977365 1107 12-13 0.19090912681782157 1107 14-15 0.21093274214133118 1107 16-17 0.10749206900074881 1107 18-19 0.10279516969310976 1107 20-21 0.08741942812733328 1107 22-23 0.19174987177826353 1107 24-25 0.16498681158042672 1107 26-27 0.17499763197111662 1107 28-29 0.25632324407122553 1107 30-31 0.14666334734450714 1107 32-33 0.42085462759036574 1107 34-35 0.373227482101413 1107 36-37 0.2539079024145039 1107 38-39 0.1951101587992241 1107 40-41 0.3032740882482017 1107 42-43 0.4110443972319828 1107 44-45 0.10540367353861768 1107 46-47 0.07021762745270621 1107 48-49 0.4203161633721493 1107 50-51 0.20148500382246226 1107 52-53 0.25706355168286166 1107 54-55 0.1440154565583427 1107 56-57 0.3546266936386573 1107 58-59 0.4816795964365568 1107 60-61 0.5384425990844548 1107 62-63 0.429439027415647 1107 64-65 0.676507195119143 1107 66-67 0.6535898941387543 1107 68-69 0.6669937819593272 1107 70-71 0.6060706526344717 1107 72-73 0.6354537695124485 1107 74-75 0.879862365980788 1107 76-77 0.9787656734395931 1107 78-79 1.0667444714725285 1107 80-81 1.037859830496263 1107 82-83 1.0764087801699134 1107 84-85 1.1145765280848536 1107 86-87 1.3006578618387792 1107 88-89 1.141255550087795 1107 90-91 1.1766827588112676 1107 92-93 1.110374180509627 1107 94-95 0.836019874581968 1107 96-97 1.000976775306988 1107 98-99 1.197580888240335 1107 100-101 1.6520285971057014 1108 1 0.15061023192815526 1108 2 0.1483446040599361 1108 3 0.02557575017082314 1108 4 0.11315054808405023 1108 5 0.08018430628565199 1108 6 3.361805156245623E-4 1108 7 0.1406300360996724 1108 8 0.036610315514359115 1108 9 0.05000057878209674 1108 10-11 0.11603795319746979 1108 12-13 0.16271498253460237 1108 14-15 0.10009091853321905 1108 16-17 -0.1273926690871363 1108 18-19 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-0.3417993803950665 2316 68-69 -0.32119584192343353 2316 70-71 -0.29268053832782215 2316 72-73 -0.18273449327054792 2316 74-75 -0.1773888930365004 2316 76-77 -0.0741130525710254 2316 78-79 -0.1469623703223064 2316 80-81 -0.31171028882681284 2316 82-83 -0.47426382996573935 2316 84-85 -0.26631873528795325 2316 86-87 0.03127069700959595 2316 88-89 0.3398371707039196 2316 90-91 0.15078783571340537 2316 92-93 0.019308099115857402 2316 94-95 0.12107102924827373 2316 96-97 0.2882595664689127 2316 98-99 0.44767446384942744 2316 100-101 0.7921538182844152 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 3.0 5 25.0 6 42.0 7 92.0 8 200.0 9 389.0 10 605.0 11 1030.0 12 1531.0 13 2029.0 14 2686.0 15 3531.0 16 4717.0 17 6397.0 18 5829.0 19 3961.0 20 3547.0 21 3707.0 22 3865.0 23 4087.0 24 4568.0 25 5309.0 26 6548.0 27 8024.0 28 10036.0 29 12555.0 30 15891.0 31 19942.0 32 25902.0 33 34202.0 34 48093.0 35 70887.0 36 111200.0 37 162356.0 38 104378.0 39 5409.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.099833985037016 18.866857730513434 14.971636105586095 22.061672178863454 2 16.14656174215445 21.98224280470257 36.37716305880521 25.49403239433777 3 21.761070852881723 22.782585376122082 30.248508459034166 25.20783531196203 4 14.189340188616404 16.44950740869435 35.56346303294371 33.797689369745534 5 14.02128036937115 37.77705359601905 33.44412352938632 14.757542505223482 6 33.35414329750944 34.9856829368867 15.48160408778825 16.178569677815617 7 28.57587755090271 32.62646739083419 20.08570052683332 18.711954531429775 8 26.29365406630545 32.139860260850256 19.697568261969092 21.868917410875202 9 24.960566799985003 18.082465130093514 21.066123781319476 35.890844288602004 10-11 25.918858441300042 25.569366387053797 26.254797585851914 22.256977585794242 12-13 27.515373912291203 23.11486637492881 26.509599232926483 22.860160479853505 14-15 23.52640943051827 25.402045624284508 26.47661834717941 24.594926598017818 16-17 22.430786443629987 28.076222140840684 26.835265824463868 22.65772559106546 18-19 22.04971899339368 27.98416328084224 28.09799330428589 21.868124421478193 20-21 23.31656755464978 25.514669996272936 28.081585926838535 23.087176522238746 22-23 22.909843540597766 25.399943624728127 28.40780227995441 23.2824105547197 24-25 24.413043666321595 25.856861088442827 27.704454293532955 22.02564095170262 26-27 23.45905964790865 25.01492279077815 29.514756981991724 22.011260579321483 28-29 22.261279797221018 27.746433993739572 28.058806481891335 21.933479727148075 30-31 22.737457070437646 25.711960298625392 27.985605079745895 23.56497755119107 32-33 22.90621963211059 26.196476820199027 28.391543561070275 22.505759986620106 34-35 24.524062181903115 25.46512432631946 27.49114014573703 22.519673346040385 36-37 23.455752378261007 25.055797939369125 28.87911023192911 22.60933945044076 38-39 22.868011961163702 26.25393250650972 28.608318026234976 22.269737506091598 40-41 23.111021719321315 26.35392516465391 27.93342041823823 22.601632697786545 42-43 22.580845192695254 26.674136676667537 26.58314099499668 24.161877135640527 44-45 23.678447124908804 25.469017183359334 26.97879690532283 23.873738786409028 46-47 24.242118406293166 26.769231434676417 26.8413934698044 22.147256689226012 48-49 24.03536444350888 26.380089910579628 26.78674929135584 22.79779635455565 50-51 22.233548353609834 27.020536983583675 28.185582587683 22.56033207512349 52-53 23.862853204686424 27.55984186349625 25.507801573867685 23.069503357949646 54-55 22.859937887303232 26.23800062862432 26.673928527144746 24.228132956927702 56-57 23.921173970339314 26.490099166928594 26.401212264518193 23.187514598213898 58-59 25.404785042201457 25.790249979093915 25.984460291416394 22.820504687288235 60-61 23.761927281430495 26.349667953712487 27.87855151114943 22.009853253707586 62-63 22.643884321590363 26.62930485107659 27.83659516305304 22.89021566428001 64-65 22.826704422573957 27.846327305652718 26.57732800059979 22.749640271173536 66-67 23.319150838117704 26.48130419361629 25.969537672763554 24.230007295502453 68-69 22.76564423900412 26.302088589891838 26.92429690676463 24.007970264339413 70-71 24.271963643598845 26.576390831312413 25.982802222677194 23.168843302411553 72-73 23.765748048525186 25.36124271531104 27.929086562722578 22.9439226734412 74-75 22.686849928919315 28.481583902603603 25.873946405451154 22.95761976302593 76-77 23.476523188451768 26.35586768899821 26.17989613280698 23.98771298974304 78-79 23.472630331411896 26.57220961449181 26.591385539910434 23.36377451418586 80-81 23.875036405422318 26.4779159661927 26.27938025715925 23.367667371225732 82-83 23.346778117802263 26.12721721797492 27.244467993843514 23.281536670379303 84-85 23.0344676445908 27.17033988967355 26.53371358376419 23.26147888197146 86-87 23.02646566067551 27.09406872767014 26.35074930289023 23.528716308764118 88-89 22.89281090230659 26.968127593435774 25.16371626551015 24.975345238747483 90-91 23.336306135676583 25.85191695283194 26.06157521995012 24.750201691541356 92-93 23.847924523133067 26.82522941302419 26.41639878684504 22.910447276997708 94-95 23.43795506777897 26.44706146965446 26.26986438439512 23.845119078171454 96-97 21.795241486898373 26.80196026978941 27.622271756024556 23.78052648728766 98-99 22.730955889349946 27.26428614682788 25.484158864086602 24.520599099735573 100-101 21.926904550242575 26.55876324189012 26.36524941581752 25.149082792049782 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 408.0 1 281.0 2 162.0 3 171.0 4 165.0 5 172.5 6 267.0 7 476.0 8 656.5 9 770.0 10 784.5 11 725.5 12 773.0 13 869.0 14 919.5 15 963.5 16 999.5 17 991.0 18 1007.0 19 1051.5 20 1063.5 21 1153.0 22 1330.5 23 1468.0 24 1596.5 25 1835.0 26 2220.0 27 2726.5 28 3183.5 29 3651.0 30 4454.0 31 5500.5 32 6431.5 33 6999.0 34 7813.5 35 8893.5 36 10347.5 37 11852.5 38 13242.0 39 14332.0 40 14811.5 41 16677.5 42 19105.5 43 21087.5 44 22749.0 45 23543.0 46 24753.5 47 34115.0 48 51624.5 49 50643.0 50 35934.0 51 29371.5 52 26964.5 53 27049.0 54 27252.5 55 24470.5 56 20667.0 57 17747.5 58 15766.5 59 14197.5 60 11965.5 61 9597.5 62 7504.0 63 5738.0 64 4262.5 65 3223.5 66 2363.5 67 1861.0 68 1494.5 69 1166.0 70 866.0 71 573.5 72 493.0 73 417.0 74 308.5 75 249.0 76 180.0 77 118.5 78 70.0 79 38.5 80 20.5 81 13.0 82 6.5 83 8.5 84 5.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03849603072761824 2 0.0 3 0.0 4 1.4417989036561137E-4 5 0.010813491777420853 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0047579363820651754 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.604497259140284E-4 22-23 0.0016580687392045307 24-25 0.0 26-27 0.001153439122924891 28-29 0.0021626983554841705 30-31 0.0 32-33 0.0 34-35 0.0 36-37 5.767195614624455E-4 38-39 0.0 40-41 2.8835978073122275E-4 42-43 0.019968914815637173 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.208994518280569E-5 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.009876322490044378 92-93 0.002090608410301365 94-95 0.0 96-97 0.0 98-99 5.767195614624455E-4 100-101 0.012615740406990995 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 693578.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.345352476131566 #Duplication Level Percentage of deduplicated Percentage of total 1 65.58029202485486 23.835388291309894 2 15.477011312205033 11.250348628383348 3 6.845099976957098 7.463627141905972 4 3.4644549602836534 5.036673466767671 5 2.102931933403417 3.821590117643001 6 1.3725282882244558 2.9931014651387606 7 0.9379524074075348 2.38631475971441 8 0.6588824917760537 1.9157853123162025 9 0.4850347072305079 1.5865881657705079 >10 2.726217270721519 18.329640884073147 >50 0.23080420718361422 5.808893675374579 >100 0.1068316526631376 6.524175413449399 >500 0.003986255696385729 1.0625636224431585 >1k 0.0071752602534943125 5.654304675905256 >5k 3.986255696385729E-4 0.7561384708932157 >10k+ 3.986255696385729E-4 1.5748659089115262 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10870 1.5672354082741955 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5219 0.7524748478181257 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4353 0.6276150627615062 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3334 0.4806957544789483 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2847 0.41048014787089554 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2773 0.3998108359838403 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2605 0.37558861440241764 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2447 0.35280819172465105 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2409 0.3473293558907578 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2367 0.34127380049540207 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2214 0.31921427726946355 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2134 0.3076798860402147 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1847 0.2663002575052842 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1741 0.2510171891265294 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1645 0.23717591965143067 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1441 0.20776322201684597 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1328 0.1914708944055319 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1270 0.18310846076432644 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1183 0.17056481030251824 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1089 0.15701190060815076 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 977 0.14086375288720232 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 955 0.13769179529915884 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 928 0.13379893825928735 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 746 0.10755819821274609 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 718 0.10352116128250896 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.4417989036561137E-4 0.0 10-11 0.0 0.0 0.0 1.4417989036561137E-4 0.0 12-13 0.0 0.0 0.0 1.4417989036561137E-4 0.0 14-15 0.0 0.0 0.0 1.4417989036561137E-4 0.0 16-17 0.0 0.0 0.0 1.4417989036561137E-4 0.0 18-19 0.0 0.0 0.0 1.4417989036561137E-4 0.0 20-21 0.0 0.0 0.0 3.604497259140284E-4 0.0 22-23 0.0 0.0 0.0 0.0010092592325592795 0.0 24-25 0.0 0.0 0.0 0.001369708958473308 0.0 26-27 0.0 0.0 0.0 0.0018743385747529476 0.0 28-29 0.0 0.0 0.0 0.0044695766013339525 0.0 30-31 0.0 0.0 0.0 0.012687830352173801 0.0 32-33 0.0 0.0 0.0 0.02256415284221818 0.0 34-35 0.0 0.0 0.0 0.03236838538707976 0.0 36-37 0.0 0.0 0.0 0.04000991957645715 0.0 38-39 0.0 0.0 0.0 0.04556084535553319 0.0 40-41 0.0 0.0 0.0 0.05298610970936218 0.0 42-43 0.0 0.0 0.0 0.06293452214458936 0.0 44-45 0.0 0.0 0.0 0.07180158540207446 0.0 46-47 0.0 0.0 0.0 0.08549867498680755 0.0 48-49 0.0 0.0 0.0 0.0989074047908094 0.0 50-51 0.0 0.0 0.0 0.11469510278584384 0.0 52-53 0.0 0.0 0.0 0.15131679493870914 0.0 54-55 0.0 0.0 0.0 0.20149139678594188 0.0 56-57 0.0 0.0 0.0 0.27120237377771494 0.0 58-59 0.0 0.0 0.0 0.36463094273463115 0.0 60-61 0.0 0.0 0.0 0.49244641554374563 0.0 62-63 0.0 0.0 0.0 0.5778009106401876 0.0 64-65 0.0 0.0 0.0 0.662578686175167 0.0 66-67 0.0 0.0 0.0 0.824708972891297 0.0 68-69 0.0 0.0 0.0 1.0274979886905293 0.0 70-71 0.0 0.0 0.0 1.2018835660877363 0.0 72-73 0.0 0.0 0.0 1.3703577679799532 0.0 74-75 0.0 0.0 0.0 1.6733518075832854 0.0 76-77 0.0 0.0 0.0 1.9060581506333822 0.0 78-79 0.0 0.0 0.0 2.120237377771498 0.0 80-81 0.0 0.0 0.0 2.330379567979376 0.0 82-83 0.0 0.0 0.0 2.6415918613335485 0.0 84-85 0.0 0.0 0.0 2.9607340486578293 0.0 86-87 0.0 0.0 0.0 3.401564063450686 0.0 88-89 0.0 0.0 0.0 3.972228069517776 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCG 35 2.7859167E-4 54.29066 4 CTAGGCG 50 0.0016146514 38.00346 3 GCGTCAG 80 4.483744E-4 29.696627 1 TATATAG 125 7.3801493E-6 26.60242 2 GAGTACT 2685 0.0 22.731567 12-13 GTTCTAG 130 2.933774E-4 21.92982 1 GTGTAAG 175 4.034644E-6 21.720963 1 AGTACTT 2705 0.0 21.597742 12-13 ATCTAGT 110 0.0028711013 21.597548 1 AAGTGCG 110 0.0028771148 21.589762 9 GTACTTT 2890 0.0 21.283472 14-15 TGCTAGC 165 6.313665E-5 20.153349 2 GTACATG 1905 0.0 19.95364 1 TACTTTT 2955 0.0 19.850891 14-15 TAAGGGT 120 0.0047591203 19.79347 4 GCATAAG 265 1.845001E-8 19.723042 1 AGAGTAC 4055 0.0 19.56123 10-11 TACATGG 1880 0.0 19.203876 2 GTCTAGG 150 7.7008957E-4 19.005842 1 TGGACCG 150 7.71211E-4 19.001732 5 >>END_MODULE