FastQCFastQC Report
Thu 26 May 2016
SRR937852_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937852_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597165
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT67231.1258194971239104No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58040.9719256821816415No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42510.7118635552987868No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27200.4554855023318513No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23500.393526077382298No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21900.36673281253924794No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC21480.3596995805179473No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC19780.3312317366222066No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT19750.3307293629063994No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC19600.32821749432736347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19150.32068188859025565No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA17410.2915442130734387No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG17310.28986963402074806No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA16960.2840086073363308No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC15050.2520241474299398No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT13550.22690546163958036No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG13300.22271901400785377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11780.1972654124069562No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA11680.19559083335426558No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC10150.16996977384809892No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC9730.1629365418267983No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC9030.15121448845796387No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8780.14702804082623733No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG7120.11923002855157286No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG6710.11236425443554128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG1250.028.49418494-95
GCATAAG1852.8558134E-1028.2707061
GTACATG15350.024.7797741
TACATGG15100.024.2392962
GATAGCG1601.8535666E-623.7551237
GAGTACT18800.022.99022112-13
GCTTATA2903.6379788E-1222.9533331
ACATGGG16300.022.1631873
GTCTAGC1302.9199471E-421.9443951
AGTTGTA2606.220944E-1021.9278057
GCACCGT1100.002869358521.5991956
TATATGT1353.7724435E-421.1280884
ATAGCGT1805.152213E-621.1156658
CATGGGG10650.020.9792984
ATATTCG2551.1870725E-820.4928959
GTACTTT21000.020.4660614-15
CCTATAA3750.020.2863751
TATAAGC3850.019.7544733
TATAGCT1456.1115646E-419.6693252
CATAAGG1951.0248881E-519.5012112
Produced by FastQC (version 0.11.5)