##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937852_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 597165 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91210804384048 33.0 31.0 34.0 30.0 34.0 2 32.005921311530315 33.0 31.0 34.0 30.0 34.0 3 32.06079056877078 34.0 31.0 34.0 30.0 34.0 4 35.64553515360076 37.0 35.0 37.0 35.0 37.0 5 35.63234449440272 37.0 35.0 37.0 35.0 37.0 6 35.71244463423007 37.0 35.0 37.0 35.0 37.0 7 35.642040307117796 37.0 35.0 37.0 33.0 37.0 8 35.661892441787444 37.0 35.0 37.0 33.0 37.0 9 37.25729572228781 39.0 37.0 39.0 34.0 39.0 10-11 37.17448946271131 39.0 37.0 39.0 34.0 39.0 12-13 37.09689533043631 39.0 37.0 39.0 33.5 39.0 14-15 38.257395359741444 40.0 38.0 41.0 33.5 41.0 16-17 38.17100801286077 40.0 38.0 41.0 33.0 41.0 18-19 38.09226093290799 40.0 38.0 41.0 33.5 41.0 20-21 38.10344377182186 40.0 38.0 41.0 33.5 41.0 22-23 38.107296140932576 40.0 38.0 41.0 34.0 41.0 24-25 38.01674160407927 40.0 38.0 41.0 33.0 41.0 26-27 37.90999807423409 40.0 38.0 41.0 33.0 41.0 28-29 37.78076494771127 40.0 38.0 41.0 33.0 41.0 30-31 37.582866544422394 40.0 37.0 41.0 33.0 41.0 32-33 37.453048152520665 40.0 37.0 41.0 32.5 41.0 34-35 37.42468413252619 40.0 37.0 41.0 33.0 41.0 36-37 37.27526311823365 40.0 37.0 41.0 32.0 41.0 38-39 37.103447120979965 40.0 37.0 41.0 31.0 41.0 40-41 36.92287140070165 39.5 36.0 41.0 31.0 41.0 42-43 36.657196084834176 39.0 36.0 41.0 30.5 41.0 44-45 36.41067125501327 39.0 36.0 41.0 30.0 41.0 46-47 36.0585382599449 39.0 35.0 40.5 29.5 41.0 48-49 35.70470221797996 39.0 35.0 40.0 28.0 41.0 50-51 34.775862617534514 38.0 34.0 39.5 26.5 40.5 52-53 34.903486473587705 38.0 34.5 39.5 27.0 40.5 54-55 35.187488382607825 38.0 35.0 40.0 27.0 41.0 56-57 34.87947049810354 38.0 34.0 40.0 26.0 41.0 58-59 34.58417439066255 38.0 34.0 40.0 26.0 41.0 60-61 34.16630244572271 37.5 34.0 40.0 25.0 41.0 62-63 33.800985489772515 37.0 33.0 40.0 23.0 41.0 64-65 33.34833337519781 36.5 33.0 39.0 22.5 41.0 66-67 32.753299339378565 36.0 32.0 39.0 20.5 40.0 68-69 32.25589493691023 35.0 31.5 38.5 19.5 40.0 70-71 31.70474240787722 35.0 31.0 37.5 18.0 39.5 72-73 31.21021576951094 35.0 31.0 37.0 15.0 39.0 74-75 30.67930304019827 34.5 30.0 36.5 9.5 39.0 76-77 30.1557031976087 34.0 30.0 36.0 7.0 38.0 78-79 29.628569993217955 34.0 29.0 35.0 3.5 37.0 80-81 29.070534944278382 34.0 29.0 35.0 2.0 37.0 82-83 28.55358904155468 34.0 28.5 35.0 2.0 36.0 84-85 28.019052523171986 33.0 27.0 35.0 2.0 36.0 86-87 27.527300662296014 33.0 26.0 35.0 2.0 35.0 88-89 26.970001590850103 32.5 25.5 35.0 2.0 35.0 90-91 26.401878877697122 32.0 24.5 34.5 2.0 35.0 92-93 25.723162777456814 32.0 23.5 34.0 2.0 35.0 94-95 24.957102308407222 31.5 19.0 34.0 2.0 35.0 96-97 24.065363006874147 31.0 9.0 34.0 2.0 35.0 98-99 22.87347801696349 31.0 2.0 34.0 2.0 35.0 100-101 19.64836100575218 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1726371938728981 1101 2 0.14767098165243908 1101 3 0.14171708437984165 1101 4 0.09113937357606261 1101 5 -0.09368424035972112 1101 6 0.05229123950145009 1101 7 -0.05162853102892484 1101 8 0.020902914900332803 1101 9 0.2428430727605999 1101 10-11 0.33785694296778246 1101 12-13 0.16684196894161119 1101 14-15 0.22685181917716335 1101 16-17 0.23467965947007485 1101 18-19 0.37501325033367294 1101 20-21 0.2978501099602937 1101 22-23 0.28302735554191827 1101 24-25 0.1508540419458413 1101 26-27 0.39133778690351306 1101 28-29 0.24468546163009108 1101 30-31 0.3447644065239359 1101 32-33 0.2331826619804005 1101 34-35 0.5331504294245732 1101 36-37 0.5565022627049885 1101 38-39 0.25205254565507573 1101 40-41 0.5306887703920324 1101 42-43 0.6141637280650656 1101 44-45 0.6202974906636172 1101 46-47 0.5524176819677464 1101 48-49 0.6443697589058459 1101 50-51 0.5663375412493323 1101 52-53 0.7924271046456752 1101 54-55 1.1000572741503305 1101 56-57 1.2656487054292853 1101 58-59 1.0589011510837665 1101 60-61 1.2740130130330272 1101 62-63 1.1542121311472613 1101 64-65 1.2678522973144837 1101 66-67 1.606149277202384 1101 68-69 1.4585615370909082 1101 70-71 1.1551430104727913 1101 72-73 1.1941285803077406 1101 74-75 1.312980109067528 1101 76-77 1.0517011241703287 1101 78-79 1.3167031470697275 1101 80-81 1.7975827039721608 1101 82-83 1.8614754819707748 1101 84-85 1.9614135663113466 1101 86-87 2.328313269148815 1101 88-89 2.3292168680707803 1101 90-91 2.547370145117778 1101 92-93 2.718285910337631 1101 94-95 2.8484243678268406 1101 96-97 2.9550782440127854 1101 98-99 3.1813239710670373 1101 100-101 4.255431066483268 1102 1 0.14174444073203674 1102 2 0.10527352762132125 1102 3 0.12040291514779256 1102 4 0.21406765379904868 1102 5 0.12302056037245279 1102 6 0.23352719923187237 1102 7 0.1788066133258468 1102 8 0.10465156287437338 1102 9 0.37301821212327724 1102 10-11 0.26025793306678935 1102 12-13 0.09265006445704671 1102 14-15 0.14982482025877886 1102 16-17 0.2092615714427879 1102 18-19 0.1607826571062816 1102 20-21 0.18222588997527112 1102 22-23 0.24522678977105983 1102 24-25 0.1957829045799997 1102 26-27 0.30153102261363784 1102 28-29 0.3046987738856899 1102 30-31 0.4372741827116471 1102 32-33 0.42047257794678927 1102 34-35 0.5021588743867156 1102 36-37 0.42283057620860376 1102 38-39 0.5578007360203259 1102 40-41 0.5343839613396995 1102 42-43 0.5355756591741425 1102 44-45 0.6988491588556442 1102 46-47 0.8468161344180274 1102 48-49 0.8723889368740672 1102 50-51 0.5765411771591502 1102 52-53 0.5962512580694224 1102 54-55 0.6888916197697696 1102 56-57 0.8473017131670346 1102 58-59 0.977487347938748 1102 60-61 0.8260972130496711 1102 62-63 1.0120102008702077 1102 64-65 0.7980045562378635 1102 66-67 0.9147034414423345 1102 68-69 0.8837653810047073 1102 70-71 1.1145780716259637 1102 72-73 1.3157672357699255 1102 74-75 1.2180283659940585 1102 76-77 1.2602896090692361 1102 78-79 1.8006733192970366 1102 80-81 1.741962436063016 1102 82-83 1.782910293519155 1102 84-85 2.06784088971596 1102 86-87 2.198500056735135 1102 88-89 2.0391303384094144 1102 90-91 2.1521999978509534 1102 92-93 2.133897650083039 1102 94-95 2.4037783906240797 1102 96-97 2.178420452183179 1102 98-99 2.6301206679636167 1102 100-101 3.400968340798933 1103 1 0.2612397312953867 1103 2 0.2674336484672395 1103 3 0.3343312961678748 1103 4 0.11780410306153044 1103 5 0.09201380721801655 1103 6 0.2497747556562544 1103 7 0.14347695276071448 1103 8 0.1884484350975839 1103 9 0.3342808742878418 1103 10-11 0.39534101463779336 1103 12-13 0.4212465634796132 1103 14-15 0.6181449730933295 1103 16-17 0.6289790052440978 1103 18-19 0.7003485892193098 1103 20-21 0.5676777293212751 1103 22-23 0.5184141009493075 1103 24-25 0.492121984018425 1103 26-27 0.5341660857023172 1103 28-29 0.5047787454513539 1103 30-31 0.6368254533709603 1103 32-33 0.6122288423861022 1103 34-35 0.5631749575571376 1103 36-37 0.7526333130132983 1103 38-39 0.8900403841454363 1103 40-41 0.7792102453276115 1103 42-43 0.6450300753817118 1103 44-45 0.7779388158664844 1103 46-47 0.7900585371192932 1103 48-49 0.9715425711374408 1103 50-51 0.7383880760395485 1103 52-53 0.8007363300438328 1103 54-55 0.9341644116659822 1103 56-57 0.7261825555341872 1103 58-59 0.8565814478143494 1103 60-61 0.9686687431722945 1103 62-63 1.3312078194946935 1103 64-65 1.2620706660654761 1103 66-67 1.359716325750135 1103 68-69 1.5704412295918786 1103 70-71 1.5323228734742003 1103 72-73 1.3124928444424029 1103 74-75 1.423586389807813 1103 76-77 1.6066864457495313 1103 78-79 1.6417276111043932 1103 80-81 1.668122023765168 1103 82-83 1.8468120173506826 1103 84-85 2.1317825993303146 1103 86-87 2.1128727242311385 1103 88-89 2.299109012658569 1103 90-91 2.255789832576383 1103 92-93 2.4809142663972388 1103 94-95 2.8840254139046912 1103 96-97 2.6949390233578754 1103 98-99 3.1571088499433593 1103 100-101 3.590943016469808 1104 1 0.13730300998105704 1104 2 0.12969715874523757 1104 3 0.03101064527154307 1104 4 -0.052329193124158735 1104 5 0.017142445437720255 1104 6 0.18593014888435988 1104 7 0.20709954433110056 1104 8 0.23076192056211653 1104 9 0.11965747301495355 1104 10-11 0.27138905698610216 1104 12-13 0.13608327347552063 1104 14-15 0.2946862374481327 1104 16-17 0.3822569943958243 1104 18-19 0.4174511602424076 1104 20-21 0.27697711899713795 1104 22-23 0.2797368762263872 1104 24-25 0.13386563046574906 1104 26-27 0.01646363831120823 1104 28-29 0.09685846196841652 1104 30-31 0.08469323678860263 1104 32-33 0.1868673058667767 1104 34-35 0.3602682997130344 1104 36-37 0.14788567029234656 1104 38-39 0.3602185186953619 1104 40-41 0.5052413100428623 1104 42-43 0.2524005447479709 1104 44-45 0.1588233070163554 1104 46-47 0.26580664128360354 1104 48-49 0.47709948103409516 1104 50-51 0.3319848964336032 1104 52-53 0.23696790495872477 1104 54-55 0.4139806179050396 1104 56-57 0.28146216409091807 1104 58-59 0.31542810060869186 1104 60-61 0.4523898050144055 1104 62-63 0.41680338615584844 1104 64-65 0.4469026745629989 1104 66-67 0.5726942025882877 1104 68-69 0.354857939065397 1104 70-71 0.7057036861368999 1104 72-73 0.2686448421438854 1104 74-75 0.2323485897608002 1104 76-77 0.594070758416585 1104 78-79 0.8399871860541133 1104 80-81 1.2028456366054812 1104 82-83 0.8977645060975625 1104 84-85 1.2215703353847118 1104 86-87 1.2793993617020796 1104 88-89 1.435985737629622 1104 90-91 1.4545478534196938 1104 92-93 1.844740589564303 1104 94-95 2.195810938513553 1104 96-97 2.302492328772434 1104 98-99 2.3117467910353255 1104 100-101 3.1016019227619793 1105 1 -0.0284349106293611 1105 2 0.05397021008577241 1105 3 -0.1278709441589676 1105 4 -0.1478898780288489 1105 5 -0.1531549809341186 1105 6 -0.10651787092889009 1105 7 0.010011891645703486 1105 8 -0.06725857558859616 1105 9 -0.11726382486213538 1105 10-11 -0.09063069252562173 1105 12-13 0.016684773127238373 1105 14-15 0.07454952056544073 1105 16-17 -0.04257375984612821 1105 18-19 0.14125961092098294 1105 20-21 0.14808059678689744 1105 22-23 0.032391086769479216 1105 24-25 0.03984944114723987 1105 26-27 0.08485622473239829 1105 28-29 -0.024703698003541774 1105 30-31 -0.1141267093987608 1105 32-33 0.029504034131157653 1105 34-35 0.13595336374643097 1105 36-37 0.2949985903336838 1105 38-39 0.22567895365808965 1105 40-41 0.4006754305340081 1105 42-43 0.2585056842647475 1105 44-45 0.2808919522763347 1105 46-47 0.38025865834703865 1105 48-49 0.4565179676332889 1105 50-51 0.2903119698364094 1105 52-53 0.2691359806788398 1105 54-55 0.31166831338559575 1105 56-57 0.25150555110031547 1105 58-59 0.7079712713659347 1105 60-61 0.9153082529158496 1105 62-63 0.6291345387570146 1105 64-65 0.37312768605850266 1105 66-67 1.0053327974501443 1105 68-69 1.1384373445768716 1105 70-71 0.9206788057584845 1105 72-73 0.8412736942775041 1105 74-75 0.8680399820148743 1105 76-77 0.9960971548363595 1105 78-79 0.9530836436163348 1105 80-81 1.0034622966707651 1105 82-83 1.1249533192602605 1105 84-85 1.3337687774587295 1105 86-87 1.5642444113490193 1105 88-89 1.359077369354491 1105 90-91 1.4170406260453596 1105 92-93 1.4025982819945675 1105 94-95 1.368400637627147 1105 96-97 1.3452590359579233 1105 98-99 1.268940247928306 1105 100-101 1.806038733885078 1106 1 -0.04278714968191011 1106 2 -0.03290730026539279 1106 3 0.007494418415767257 1106 4 0.13678007209969678 1106 5 -0.054702751174389164 1106 6 0.09567316873078369 1106 7 -0.07740253700094968 1106 8 0.11211343651271477 1106 9 0.09725568489015757 1106 10-11 -0.0030045941663203735 1106 12-13 -0.14980636255820912 1106 14-15 -0.13821984205058868 1106 16-17 -0.052176337053950306 1106 18-19 0.31620204708449506 1106 20-21 0.24254724073779244 1106 22-23 0.36467123421549985 1106 24-25 0.08902909194384279 1106 26-27 0.28603537991211425 1106 28-29 0.23106042965910234 1106 30-31 0.6107491282454092 1106 32-33 0.6720248655284919 1106 34-35 0.5304540813583927 1106 36-37 0.3446170685833394 1106 38-39 0.5549957251270783 1106 40-41 0.7818512816011349 1106 42-43 0.8646606264943983 1106 44-45 0.76178488870314 1106 46-47 0.9146265919343719 1106 48-49 0.853734252778203 1106 50-51 0.7211217871809339 1106 52-53 0.8061368789844536 1106 54-55 0.9891094846935147 1106 56-57 0.9967842839731347 1106 58-59 0.7117193740825343 1106 60-61 0.8152039668623061 1106 62-63 0.9349404840946107 1106 64-65 0.9136908307476617 1106 66-67 1.3907110667146654 1106 68-69 1.2606172328565606 1106 70-71 0.8684996402534182 1106 72-73 0.7711593926326721 1106 74-75 1.1618736818981965 1106 76-77 1.4857253389167298 1106 78-79 1.442639550233853 1106 80-81 1.65045916808916 1106 82-83 1.523160838181468 1106 84-85 1.3180587546397042 1106 86-87 1.6434346972580123 1106 88-89 1.9564505998473223 1106 90-91 1.8984962941472503 1106 92-93 2.1660599594512284 1106 94-95 2.2767631400794173 1106 96-97 2.30946620381717 1106 98-99 2.5231762235191724 1106 100-101 2.633620846245556 1107 1 0.19610305164627917 1107 2 0.27123738304300815 1107 3 0.1816419890906218 1107 4 0.2591125392230964 1107 5 0.1556156465997134 1107 6 0.30465287841921196 1107 7 0.328889297001723 1107 8 0.18945548444300186 1107 9 0.3525776262503726 1107 10-11 0.2674366479262318 1107 12-13 0.3138259444475935 1107 14-15 0.34372099318577654 1107 16-17 0.28403123834568333 1107 18-19 0.4767199356321541 1107 20-21 0.5419737047638336 1107 22-23 0.4272549022208594 1107 24-25 0.2552560914451121 1107 26-27 0.47598471975513945 1107 28-29 0.5559744648849758 1107 30-31 0.5858390303374392 1107 32-33 0.5822933906092018 1107 34-35 0.6450037834346674 1107 36-37 0.6849850765629668 1107 38-39 0.37424002257832 1107 40-41 0.684835768568604 1107 42-43 0.898639679963182 1107 44-45 0.6830122819362074 1107 46-47 0.8465503654598479 1107 48-49 0.8027131750030563 1107 50-51 0.7018158050826102 1107 52-53 0.6784890326973425 1107 54-55 0.7205122198170173 1107 56-57 0.7910856725339173 1107 58-59 0.8923438327013287 1107 60-61 1.3082021031985036 1107 62-63 0.9908372738680811 1107 64-65 1.151701697745068 1107 66-67 1.6147182063844596 1107 68-69 1.6634901624835692 1107 70-71 1.189607601510204 1107 72-73 1.3301010415814076 1107 74-75 1.1192390912480157 1107 76-77 1.37700942952754 1107 78-79 1.4709751147139976 1107 80-81 1.6845563410949076 1107 82-83 1.9792663058035878 1107 84-85 2.182405028624533 1107 86-87 2.395541162334002 1107 88-89 2.251119471412011 1107 90-91 2.7540426626277075 1107 92-93 3.1103534733762075 1107 94-95 3.1443052037589716 1107 96-97 3.0531664951786937 1107 98-99 3.2794562455681557 1107 100-101 3.6457149743921278 1108 1 0.011380759990029077 1108 2 0.1443814114387436 1108 3 0.03498065495740832 1108 4 0.09997807605923725 1108 5 -0.08816445305200205 1108 6 0.01633779234878574 1108 7 -0.010551453552359646 1108 8 0.20466986895198858 1108 9 0.181969884078363 1108 10-11 0.27825585837799593 1108 12-13 0.13381127998059839 1108 14-15 0.2261886230413026 1108 16-17 0.4009262041008199 1108 18-19 0.4525235650877306 1108 20-21 0.2618115624185222 1108 22-23 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-2.3116385179254983 2316 88-89 -2.379011629916377 2316 90-91 -3.511770590384341 2316 92-93 -3.4038427345323505 2316 94-95 -3.0608567903713926 2316 96-97 -2.7249025116177243 2316 98-99 -2.96273849493231 2316 100-101 -2.6802957994725602 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2395.0 3 418.0 4 344.0 5 400.0 6 437.0 7 521.0 8 631.0 9 834.0 10 1028.0 11 1293.0 12 1918.0 13 2353.0 14 2689.0 15 3522.0 16 4331.0 17 5753.0 18 4990.0 19 3776.0 20 3916.0 21 4398.0 22 4769.0 23 5186.0 24 5887.0 25 6700.0 26 7899.0 27 9472.0 28 11090.0 29 13416.0 30 16913.0 31 20937.0 32 26736.0 33 34905.0 34 47442.0 35 66815.0 36 93474.0 37 114846.0 38 61486.0 39 3245.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.21704806299509 18.036982392697624 14.88033823227927 22.865631312028015 2 15.535356792561839 20.618189635481748 38.05070740457092 25.79574616738549 3 20.770685531548963 22.410781468343547 31.024266365688487 25.794266634419007 4 13.32604427515019 15.700026704153252 36.63373154432302 34.34019747637354 5 13.72699515298496 36.59748277680554 34.64305748230655 15.032464587902952 6 33.00982605120287 33.93184697876197 16.390793216584292 16.667533753450865 7 27.524349069331922 32.28559058659275 21.12076198658871 19.069298357486623 8 25.535517695075455 31.40808764298373 20.776274099135385 22.280120562805436 9 24.569155765148146 17.459323840131574 22.133201312377803 35.83831908234248 10-11 25.467814371257486 24.825964662603045 27.076256679466184 22.62996428667329 12-13 26.631668427487604 22.909189471325753 27.463771703621532 22.995370397565114 14-15 23.250677794438854 24.57874372867077 27.19921324659131 24.97136523029907 16-17 21.966815597927546 27.627713800264303 27.56822520084744 22.83724540096071 18-19 21.588934577181004 27.238602794042755 29.104206501758128 22.068256127018113 20-21 22.70902061596185 25.263362787903603 28.55090897497424 23.476707621160305 22-23 22.283164560254466 24.910201646701687 29.468039673210345 23.338594119833502 24-25 23.865891282295678 25.12780669201125 28.3661635506138 22.64013847507927 26-27 22.63792510299806 24.69956451328696 30.112521711404046 22.549988672310935 28-29 21.727926183135775 26.98022834491057 28.882291437653247 22.409554034300406 30-31 21.947322532217473 25.307153760236712 28.79797795860756 23.94754574893825 32-33 22.293679370916028 25.602227404357304 29.022380613929165 23.08171261079751 34-35 23.85448509834163 25.099247889382763 27.951780336372934 23.09448667590267 36-37 22.709095821355874 24.65712161715424 29.776510250691796 22.857272310798088 38-39 22.351253397765024 25.71478908688211 29.091999731534617 22.84195778381825 40-41 22.478156734604852 25.988766919879364 28.843680658380976 22.68939568713481 42-43 22.25650964648435 25.953762317673267 27.310856332615984 24.478871703226403 44-45 22.81831049373963 25.234947305185244 27.74773610725394 24.199006093821186 46-47 23.57529908692274 26.07615074417223 27.87243433009396 22.47611583881107 48-49 23.228524196729015 25.80679539141891 27.644018159085 23.32066225276708 50-51 21.573033519304627 26.43885812675528 29.01848608907323 22.969622264866864 52-53 23.023028063170457 26.765958982337683 26.56757575528707 23.64343719920479 54-55 22.148447857476093 25.413501911439436 27.876137381982456 24.56191284910201 56-57 23.334342759020448 25.779194142758517 27.133698012829495 23.75276508539154 58-59 24.401897823809687 25.310216360972326 27.209717582547537 23.07816823267045 60-61 23.28396420458549 25.359531183416266 28.831364272364585 22.525140339633655 62-63 22.224207362746405 26.047839649854765 28.609006442759593 23.11894654463924 64-65 22.10652323086241 26.911745707915085 27.630826170770707 23.350904890451798 66-67 22.69493788965767 25.836358877651683 26.76642014911851 24.702283083572134 68-69 22.041600048317527 25.56819230375618 28.108721145796462 24.28148650212983 70-71 23.576263935375092 25.605019671003515 27.074850475207825 23.74386591841357 72-73 22.86746427981171 24.764487115060493 28.956360588704612 23.41168801642319 74-75 22.134769713521017 27.25825058625622 27.029033841352145 23.577945858870613 76-77 22.638515874673935 25.661000766025065 27.204042061740108 24.496441297560885 78-79 22.842855944290626 25.595100260088934 27.818692843359344 23.743350952261096 80-81 23.38722803061192 25.566338969456876 27.099871303758366 23.94656169617284 82-83 22.649689158593294 25.183396849391027 28.550516866638088 23.616397125377596 84-85 22.609392235070107 26.02446829481066 27.58223553145354 23.78390393866569 86-87 22.528482422312468 26.177319594547548 27.687154324773015 23.607043658366965 88-89 22.431774193954332 25.671058029583183 26.396081750996885 25.501086025465597 90-91 22.544671133710125 24.759792246404956 27.467619248164205 25.22791737172071 92-93 23.444313390590633 25.22553659841986 27.92442573524847 23.405724275741036 94-95 22.603368320738763 25.048487980349787 27.805759499617466 24.542384199293988 96-97 21.244083205111643 25.2066788260542 28.917585192617786 24.631652776216374 98-99 22.008540555052182 25.747088828484177 27.190677539514752 25.05369307694889 100-101 21.824715150376445 24.11106115711352 28.590313099727567 25.473910592782467 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 938.0 1 572.0 2 198.5 3 185.0 4 160.5 5 162.0 6 251.0 7 481.0 8 701.5 9 796.0 10 752.0 11 648.5 12 680.5 13 731.0 14 785.0 15 837.5 16 829.5 17 809.5 18 833.5 19 873.5 20 926.0 21 1048.5 22 1182.5 23 1331.0 24 1473.5 25 1704.5 26 2028.0 27 2456.5 28 2872.5 29 3287.5 30 4015.5 31 4923.5 32 5892.5 33 6442.0 34 7027.0 35 8071.0 36 9326.5 37 10516.0 38 11640.5 39 12535.0 40 12973.0 41 14643.5 42 16618.0 43 18273.5 44 19674.5 45 20450.0 46 22343.5 47 29976.5 48 42896.0 49 42710.5 50 31257.0 51 24987.0 52 22816.0 53 23167.5 54 23480.0 55 20859.5 56 17456.0 57 14882.0 58 13040.0 59 11434.5 60 9679.5 61 7935.5 62 6139.0 63 4640.5 64 3536.5 65 2690.0 66 1964.0 67 1537.5 68 1219.0 69 917.5 70 650.5 71 448.5 72 349.5 73 290.5 74 232.5 75 196.0 76 132.0 77 76.5 78 44.0 79 32.0 80 21.5 81 10.0 82 5.0 83 4.5 84 3.5 85 4.0 86 3.0 87 0.0 88 1.0 89 1.0 90 0.5 91 1.0 92 0.5 93 0.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34680532181222945 2 0.29288387631559115 3 0.2969028660420487 4 0.29338625003139834 5 0.292548960505053 6 0.2176952768497819 7 0.20915492368105965 8 0.21652307151289846 9 0.2176952768497819 10-11 0.21920239799720345 12-13 0.22196545343414298 14-15 0.21744408999187828 16-17 0.2097410263495014 18-19 0.20404745757035325 20-21 0.20413118652298778 22-23 0.20873627891788699 24-25 0.20856882101261795 26-27 0.21434611874440063 28-29 0.21970477171301064 30-31 0.2229702008657574 32-33 0.2201234164761833 34-35 0.21911866904456892 36-37 0.20999221320740497 38-39 0.19843761774383964 40-41 0.1938325253489404 42-43 0.20471728919142948 44-45 0.20655932614938918 46-47 0.2123366238811719 48-49 0.22104443495516313 50-51 0.21509967931811141 52-53 0.22581698525533145 54-55 0.21417866083913156 56-57 0.18696675123290882 58-59 0.16888129746385003 60-61 0.18604573275392897 62-63 0.17909622968526287 64-65 0.18855760133296492 66-67 0.18905997504877212 68-69 0.1855433590381218 70-71 0.1858782748486599 72-73 0.19734914135959075 74-75 0.20471728919142948 76-77 0.20622441033885108 78-79 0.2034613549019115 80-81 0.19893999145964683 82-83 0.18780404075925414 84-85 0.182110471980106 86-87 0.1908182830540973 88-89 0.1983538887912051 90-91 0.19600947811743824 92-93 0.19123692781726995 94-95 0.19106946991200088 96-97 0.1996098230807231 98-99 0.19843761774383964 100-101 0.17767283749047583 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 597165.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.96899757397744 #Duplication Level Percentage of deduplicated Percentage of total 1 68.56273616054325 27.40383835266008 2 14.813151298270865 11.84133616607098 3 6.133298389428045 7.354253653425877 4 3.1724132178785567 5.071927048361679 5 1.8482466234017862 3.6936282403428997 6 1.1089761436691494 2.6594798877546655 7 0.7755525782147702 2.169864138201066 8 0.5851413621693962 1.8710010947985956 9 0.42139548439739893 1.5158479584208218 >10 2.2990380368776555 17.201279191693445 >50 0.19287702705427867 5.335607119410328 >100 0.07538206946364084 5.049124788165098 >500 0.0033690310374811555 0.9666674553599413 >1k 0.0075803198343326 5.75758784225007 >5k 8.422577593702889E-4 2.1085570630844477 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6723 1.1258194971239104 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5804 0.9719256821816415 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4251 0.7118635552987868 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2720 0.4554855023318513 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2350 0.393526077382298 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2190 0.36673281253924794 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2148 0.3596995805179473 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1978 0.3312317366222066 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1975 0.3307293629063994 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1960 0.32821749432736347 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1915 0.32068188859025565 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1741 0.2915442130734387 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1731 0.28986963402074806 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1696 0.2840086073363308 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1505 0.2520241474299398 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1355 0.22690546163958036 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1330 0.22271901400785377 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1178 0.1972654124069562 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1168 0.19559083335426558 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1015 0.16996977384809892 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 973 0.1629365418267983 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 903 0.15121448845796387 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 878 0.14702804082623733 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 712 0.11923002855157286 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 671 0.11236425443554128 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.372895263453149E-5 0.0 18-19 0.0 0.0 0.0 1.6745790526906298E-4 0.0 20-21 0.0 0.0 0.0 3.3491581053812596E-4 0.0 22-23 0.0 0.0 0.0 3.3491581053812596E-4 0.0 24-25 0.0 0.0 0.0 7.535605737107834E-4 0.0 26-27 0.0 0.0 0.0 0.00167457905269063 0.0 28-29 0.0 0.0 0.0 0.004688821347533763 0.0 30-31 0.0 0.0 0.0 0.012391884989910663 0.0 32-33 0.0 0.0 0.0 0.022858004069227097 0.0 34-35 0.0 0.0 0.0 0.03072852561687306 0.0 36-37 0.0 0.0 0.0 0.03533361801177229 0.0 38-39 0.0 0.0 0.0 0.040189897264575114 0.0 40-41 0.0 0.0 0.0 0.046134652901626855 0.0 42-43 0.0 0.0 0.0 0.05643331407567423 0.0 44-45 0.0 0.0 0.0 0.06622960153391441 0.0 46-47 0.0 0.0 0.0 0.07753301013957617 0.0 48-49 0.0 0.0 0.0 0.08950625036631417 0.0 50-51 0.0 0.0 0.0 0.10566593822477874 0.0 52-53 0.0 0.0 0.0 0.1406646404260129 0.0 54-55 0.0 0.0 0.0 0.1850409853223146 0.0 56-57 0.0 0.0 0.0 0.24524210226654275 0.0 58-59 0.0 0.0 0.0 0.3217703649745045 0.0 60-61 0.0 0.0 0.0 0.43078546130466455 0.0 62-63 0.0 0.0 0.0 0.5084022003968752 0.0 64-65 0.0 0.0 0.0 0.5872748737786039 0.0 66-67 0.0 0.0 0.0 0.7473646312158282 0.0 68-69 0.0 0.0 0.0 0.9494025939229527 0.0 70-71 0.0 0.0 0.0 1.1266567866502557 0.0 72-73 0.0 0.0 0.0 1.28674654408748 0.0 74-75 0.0 0.0 0.0 1.6109450486883858 0.0 76-77 0.0 0.0 0.0 1.8388552577595807 0.0 78-79 0.0 0.0 0.0 2.049182386777524 0.0 80-81 0.0 0.0 0.0 2.2575837498848728 0.0 82-83 0.0 0.0 0.0 2.5780981805698593 0.0 84-85 0.0 0.0 0.0 2.919461120460844 0.0 86-87 0.0 0.0 0.0 3.3927808897038507 0.0 88-89 0.0 0.0 0.0 4.032553816784306 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 125 0.0 28.494184 94-95 GCATAAG 185 2.8558134E-10 28.270706 1 GTACATG 1535 0.0 24.779774 1 TACATGG 1510 0.0 24.239296 2 GATAGCG 160 1.8535666E-6 23.755123 7 GAGTACT 1880 0.0 22.990221 12-13 GCTTATA 290 3.6379788E-12 22.953333 1 ACATGGG 1630 0.0 22.163187 3 GTCTAGC 130 2.9199471E-4 21.944395 1 AGTTGTA 260 6.220944E-10 21.927805 7 GCACCGT 110 0.0028693585 21.599195 6 TATATGT 135 3.7724435E-4 21.128088 4 ATAGCGT 180 5.152213E-6 21.115665 8 CATGGGG 1065 0.0 20.979298 4 ATATTCG 255 1.1870725E-8 20.492895 9 GTACTTT 2100 0.0 20.46606 14-15 CCTATAA 375 0.0 20.286375 1 TATAAGC 385 0.0 19.754473 3 TATAGCT 145 6.1115646E-4 19.669325 2 CATAAGG 195 1.0248881E-5 19.501211 2 >>END_MODULE