FastQCFastQC Report
Thu 26 May 2016
SRR937852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937852_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597165
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT98121.643096966500046No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42690.7148777975936299No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40050.6706689106025973No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29780.4986896418912696No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG24120.4039084675089799No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23550.3943633669086433No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA22480.3764453710448536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22410.37527316570797015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20780.3479775271491129No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT18590.3113042458951881No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT18580.311136787989919No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA16210.2714492644411511No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15060.25219160533520885No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC14760.24716786817713696No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC14300.23946480453476007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13100.2193698559024725No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT11980.20061457051233747No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC10190.17063960546917517No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG9600.16075958905830048No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG9600.16075958905830048No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA9330.15623822561603576No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT8580.14367888272085605No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC8240.1379853139417079No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG6710.11236425443554128No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6420.10750797518273844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAG957.2759576E-1250.0177271
ATAGGAC1302.055458E-1036.539173
CTATAGG550.002574158434.55771
GAACGAT702.0519215E-433.9292266
CATAAGG2200.030.227862
TCGTGTC650.005838908729.2337849
GGACCGC856.4028206E-427.9417176
ATAAGGC2600.027.4043793
GTAGCAC1405.797265E-727.1433833
GCATAAG2950.025.7718451
ACTCGTC950.001225725625.0004863
CCTATAA3700.024.4005371
GTAAGGT2002.0494554E-823.750464
TAATGCC1801.9473919E-723.750464
TAAGGCC2853.6379788E-1223.3337864
TAATATA1050.002194747622.6194864
GTGTTAC1903.27027E-722.5079771
TATAAGC3650.022.1237163
GAGTACT20600.022.0219812-13
TTACATA2405.9117156E-921.7712554
Produced by FastQC (version 0.11.5)