##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937852_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 597165 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24821448008507 33.0 31.0 34.0 31.0 34.0 2 32.47784615642243 34.0 31.0 34.0 31.0 34.0 3 32.58998601726491 34.0 31.0 34.0 31.0 34.0 4 36.1736722681336 37.0 35.0 37.0 35.0 37.0 5 36.0166788073648 37.0 35.0 37.0 35.0 37.0 6 36.118900136478196 37.0 35.0 37.0 35.0 37.0 7 36.08601140388335 37.0 35.0 37.0 35.0 37.0 8 36.083260070499776 37.0 35.0 37.0 35.0 37.0 9 37.750095869650764 39.0 37.0 39.0 35.0 39.0 10-11 37.70022774275117 39.0 37.0 39.0 35.0 39.0 12-13 37.63250441670225 39.0 37.0 39.0 35.0 39.0 14-15 38.851438044761494 40.0 38.0 41.0 35.0 41.0 16-17 38.84778746242663 40.0 38.0 41.0 35.0 41.0 18-19 38.82276757680039 40.0 38.0 41.0 35.0 41.0 20-21 38.723687757989836 40.0 38.0 41.0 34.5 41.0 22-23 38.58354223707016 40.0 38.0 41.0 34.0 41.0 24-25 38.50573040951831 40.0 38.0 41.0 34.0 41.0 26-27 38.41183927390252 40.0 38.0 41.0 34.0 41.0 28-29 38.31990823306791 40.0 38.0 41.0 34.0 41.0 30-31 38.44919327154137 40.0 38.0 41.0 34.0 41.0 32-33 38.37477330386074 40.0 38.0 41.0 34.0 41.0 34-35 38.21623839307394 40.0 38.0 41.0 34.0 41.0 36-37 38.06062980918172 40.0 37.5 41.0 33.5 41.0 38-39 37.92521413679636 40.0 37.0 41.0 33.0 41.0 40-41 37.80085654718545 40.0 37.0 41.0 33.0 41.0 42-43 37.60133212763641 40.0 37.0 41.0 33.0 41.0 44-45 37.32957976438672 40.0 37.0 41.0 32.5 41.0 46-47 37.00478427235353 40.0 36.5 41.0 31.5 41.0 48-49 36.68900890038766 39.5 36.0 41.0 31.0 41.0 50-51 36.39628997010877 39.0 36.0 41.0 30.0 41.0 52-53 36.20554369395393 39.0 35.0 40.5 30.0 41.0 54-55 36.01247059020538 39.0 35.0 40.0 29.5 41.0 56-57 35.67520785712492 38.5 35.0 40.0 28.5 41.0 58-59 35.410619343062635 38.0 35.0 40.0 28.0 41.0 60-61 34.96649669689282 38.0 34.0 40.0 28.0 41.0 62-63 34.61928780152889 37.0 34.0 40.0 27.0 41.0 64-65 34.21073153985917 36.5 34.0 39.0 26.0 41.0 66-67 33.72572990714459 36.0 33.0 39.0 26.0 40.0 68-69 33.24777908953136 35.5 33.0 38.5 25.5 40.0 70-71 32.75305652541592 35.0 32.0 37.5 24.5 39.5 72-73 32.271517084892785 35.0 32.0 37.0 23.5 39.0 74-75 31.8012743546591 35.0 31.0 36.5 22.5 39.0 76-77 30.598351376922626 33.5 30.0 35.0 21.0 37.0 78-79 31.16542915274673 34.0 31.0 35.5 22.0 37.0 80-81 30.95565044836854 34.0 31.0 35.0 20.0 37.0 82-83 30.64095852904976 34.0 31.0 35.0 20.0 36.0 84-85 30.291550074100122 34.0 31.0 35.0 18.5 36.0 86-87 29.990486716401662 34.0 30.0 35.0 17.5 35.5 88-89 29.57091339914429 34.0 30.0 35.0 9.5 35.0 90-91 29.110555709058637 34.0 29.0 35.0 4.5 35.0 92-93 28.731974412432074 34.0 29.0 35.0 2.0 35.0 94-95 28.30261652976983 33.5 29.0 35.0 2.0 35.0 96-97 27.75805849304631 33.0 27.5 35.0 2.0 35.0 98-99 27.05766747883751 33.0 26.5 35.0 2.0 35.0 100-101 24.726552125459463 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.031056848759270395 1101 2 0.0708343529263118 1101 3 0.06685822241763617 1101 4 0.014013383999454732 1101 5 -0.20127427145325072 1101 6 -0.06424689917025717 1101 7 -0.047393098199897565 1101 8 -0.07157917497558941 1101 9 0.13340653202273955 1101 10-11 0.11046501581004264 1101 12-13 0.10269859668152748 1101 14-15 0.14091657634077848 1101 16-17 -0.042320305779796286 1101 18-19 0.04153127590383576 1101 20-21 0.18505471721299926 1101 22-23 0.10431254600223383 1101 24-25 0.17260256705815635 1101 26-27 0.008991479553870363 1101 28-29 0.21210139527236294 1101 30-31 0.2740893287391444 1101 32-33 0.09012961498471128 1101 34-35 2.5414001104451245E-4 1101 36-37 0.06596852249334972 1101 38-39 0.18294612038626212 1101 40-41 0.06932379023692903 1101 42-43 0.23852508930096405 1101 44-45 0.29884206233702315 1101 46-47 0.18051617333291148 1101 48-49 0.22846917736990946 1101 50-51 0.2886738762381782 1101 52-53 0.43694061456859856 1101 54-55 0.42526015680751783 1101 56-57 0.4975154978321612 1101 58-59 0.38281647670724084 1101 60-61 0.6026292838061522 1101 62-63 0.5080656128622181 1101 64-65 0.45907305965484824 1101 66-67 0.5600091217708538 1101 68-69 0.431457709006672 1101 70-71 0.32407644556016635 1101 72-73 0.23418801753546603 1101 74-75 0.07560314284569714 1101 76-77 -0.020871092168309957 1101 78-79 0.021013976240727317 1101 80-81 0.17984064322921256 1101 82-83 0.047442333765125966 1101 84-85 0.2057615390792975 1101 86-87 0.5814008861529345 1101 88-89 0.7152367087069784 1101 90-91 0.5205342514361782 1101 92-93 0.6110172419247206 1101 94-95 0.416947477582962 1101 96-97 0.7569239284790257 1101 98-99 0.7709477835614322 1101 100-101 1.5627000947511753 1102 1 0.22442776148079702 1102 2 0.1508971701324029 1102 3 0.13161194568912293 1102 4 0.030878347806755357 1102 5 0.057723981313202444 1102 6 -0.045195813389412365 1102 7 -0.0401628793797002 1102 8 0.03288875080858844 1102 9 0.11494825762387251 1102 10-11 0.1619928051435906 1102 12-13 0.19624319273777502 1102 14-15 0.010044623599277713 1102 16-17 0.04694252806662291 1102 18-19 0.09946974832250532 1102 20-21 0.24951099477353722 1102 22-23 0.47377651971052614 1102 24-25 0.162294929151507 1102 26-27 0.25729936706530054 1102 28-29 0.09230461517418576 1102 30-31 0.153392931368316 1102 32-33 0.3661945954323329 1102 34-35 0.34401090014083735 1102 36-37 0.30485694915113726 1102 38-39 0.2544225327333791 1102 40-41 0.3677648003043217 1102 42-43 0.26890303255747483 1102 44-45 0.16289897222969074 1102 46-47 0.10214026851554792 1102 48-49 0.04072477267733632 1102 50-51 -0.17525927127825014 1102 52-53 -0.3315010195107746 1102 54-55 -0.48593461813216976 1102 56-57 -0.3262520785053127 1102 58-59 -0.10394198905530061 1102 60-61 -0.15518854629618062 1102 62-63 -0.18885072793152347 1102 64-65 -0.22077343339782374 1102 66-67 -0.09320141987155495 1102 68-69 0.08336406560871978 1102 70-71 -0.2437546967977653 1102 72-73 -0.16806362569608524 1102 74-75 -0.10124185257821594 1102 76-77 -0.13066496021057716 1102 78-79 0.10174240622658459 1102 80-81 0.04319553964322509 1102 82-83 -0.011563199141104263 1102 84-85 0.1031448488388449 1102 86-87 0.19357119982295856 1102 88-89 0.2116049589773823 1102 90-91 0.5035351250529523 1102 92-93 0.9103672294444785 1102 94-95 0.545268261342013 1102 96-97 0.6376481983850084 1102 98-99 0.9106983867730136 1102 100-101 1.8872164854658777 1103 1 0.2196072532170632 1103 2 0.2874575042358316 1103 3 0.3529421393980243 1103 4 0.22919005919825963 1103 5 0.060354157250650076 1103 6 0.21610239169322654 1103 7 0.2630715261649996 1103 8 0.18380398094897998 1103 9 0.3918364751465262 1103 10-11 0.34142900560565437 1103 12-13 0.38243254763381174 1103 14-15 0.3438548244167663 1103 16-17 0.28093932920398856 1103 18-19 0.39928092657023484 1103 20-21 0.4693706002471387 1103 22-23 0.5807362673558032 1103 24-25 0.484009430662077 1103 26-27 0.4821771882844246 1103 28-29 0.24920660557759788 1103 30-31 0.4247097964829436 1103 32-33 0.3832418675133695 1103 34-35 0.5258195903843088 1103 36-37 0.18617025997395587 1103 38-39 0.381848336891899 1103 40-41 0.18271726165139768 1103 42-43 0.3325759488539006 1103 44-45 0.16177937031037715 1103 46-47 0.1830448357805139 1103 48-49 0.2343448188831303 1103 50-51 0.13692528532384785 1103 52-53 -0.04956570762167445 1103 54-55 0.05866285832065188 1103 56-57 0.06609064408221599 1103 58-59 -0.13076800647132814 1103 60-61 -0.09454122090076567 1103 62-63 0.05085737585380201 1103 64-65 0.21160172210244355 1103 66-67 0.25919550566603533 1103 68-69 0.010250061698997115 1103 70-71 0.26938063286279856 1103 72-73 0.11905428793462391 1103 74-75 0.20940325138481697 1103 76-77 0.34378190312690293 1103 78-79 0.4084644606072487 1103 80-81 0.2704280791847218 1103 82-83 0.180313022646704 1103 84-85 0.42610557391437 1103 86-87 0.3985605369475351 1103 88-89 0.4862095440138141 1103 90-91 0.38292245400265656 1103 92-93 0.7287362537913786 1103 94-95 0.8808400580365188 1103 96-97 1.0502081770592575 1103 98-99 1.2654855735510466 1103 100-101 1.7929899882204516 1104 1 0.08311143667997811 1104 2 0.14644302536210319 1104 3 0.16801218301748122 1104 4 0.06754557954094764 1104 5 -0.055498598785113984 1104 6 -0.14571708818174756 1104 7 0.07428024657385635 1104 8 0.06821677250717784 1104 9 0.09059667260946469 1104 10-11 0.0713736324024552 1104 12-13 0.1335948625123109 1104 14-15 0.05953606282626822 1104 16-17 0.003019829304122368 1104 18-19 0.1586556272850359 1104 20-21 0.05533401562452411 1104 22-23 0.06869303098362423 1104 24-25 0.10332518851749484 1104 26-27 -0.022012804489193627 1104 28-29 -0.04723810393074501 1104 30-31 -0.09785304252556415 1104 32-33 -0.08617445016862035 1104 34-35 -0.25509276149671933 1104 36-37 -0.24141360518010657 1104 38-39 -0.4499468519080878 1104 40-41 -0.23029631174856746 1104 42-43 -0.06740184962594498 1104 44-45 -0.32834530646160687 1104 46-47 -0.36014147415270514 1104 48-49 -0.2985700230507504 1104 50-51 -0.4250452519529375 1104 52-53 -0.5321875765543425 1104 54-55 -0.666956825285915 1104 56-57 -0.5682655553657909 1104 58-59 -0.5370492953784023 1104 60-61 -0.39582890906953594 1104 62-63 -0.5189511686841897 1104 64-65 -0.5563684495022443 1104 66-67 -0.5548580565896373 1104 68-69 -0.6167143375104587 1104 70-71 -0.7059495603339556 1104 72-73 -0.6526944953350089 1104 74-75 -0.4125658057906243 1104 76-77 -0.6334005933687372 1104 78-79 -1.0238339626494977 1104 80-81 -0.7394646570002763 1104 82-83 -0.7495802359270023 1104 84-85 -0.7036274854551046 1104 86-87 -0.5837936077715504 1104 88-89 -0.4120770210192255 1104 90-91 -0.3671950834569664 1104 92-93 -0.0807986461276542 1104 94-95 -0.2717409775120352 1104 96-97 -0.12424788569704148 1104 98-99 0.01827382328767513 1104 100-101 0.5659141460863211 1105 1 0.09708132527597968 1105 2 0.12113924800620879 1105 3 0.007903647203853836 1105 4 0.0839124524438759 1105 5 -0.015048065894305296 1105 6 0.020455613057940525 1105 7 0.04662273534012229 1105 8 -3.237759082637126E-4 1105 9 0.1196086051752161 1105 10-11 0.18081656687577663 1105 12-13 -0.02408368208799061 1105 14-15 0.09396340438173922 1105 16-17 -0.08303145204675388 1105 18-19 0.14640892683750906 1105 20-21 0.1476338458096791 1105 22-23 -0.07660564737432196 1105 24-25 -0.08420981018498708 1105 26-27 -0.036245610167291886 1105 28-29 -0.17477906663977194 1105 30-31 -0.1263812531870201 1105 32-33 -0.17111374022420733 1105 34-35 -0.21454662239424493 1105 36-37 -0.22518672708712728 1105 38-39 -0.32274733995502913 1105 40-41 -0.24800394795327207 1105 42-43 -0.04980689217070733 1105 44-45 0.06979424218601338 1105 46-47 0.1791777185666561 1105 48-49 -0.2084156417822811 1105 50-51 -0.01637650016031955 1105 52-53 -4.8159752397225475E-4 1105 54-55 -0.20511963811171796 1105 56-57 0.015713119896325622 1105 58-59 0.0037756951747880407 1105 60-61 -0.14027009076231423 1105 62-63 -0.18606172133362264 1105 64-65 -0.13468245672278556 1105 66-67 0.1250694192697921 1105 68-69 -0.10760215500275194 1105 70-71 -0.16365177885333537 1105 72-73 -0.030939984770519402 1105 74-75 -0.2625186855863042 1105 76-77 -0.2107562621341934 1105 78-79 -0.17775996344166245 1105 80-81 -0.03863584219861238 1105 82-83 0.059486192179402764 1105 84-85 -0.013047884178533309 1105 86-87 0.13490332509012504 1105 88-89 0.18269035996241811 1105 90-91 -0.042563053554985686 1105 92-93 0.39472161100752956 1105 94-95 0.41563695384270716 1105 96-97 0.5688743855423333 1105 98-99 0.8505657318152942 1105 100-101 1.067507582947524 1106 1 0.0672926852280682 1106 2 0.2309080633785925 1106 3 0.12011957096145665 1106 4 0.19710928462593102 1106 5 0.0949788832739884 1106 6 0.00932488377834062 1106 7 0.06027285046779696 1106 8 -0.014170072720823157 1106 9 -0.02916938943495495 1106 10-11 0.11664966788869435 1106 12-13 -0.2249169379811775 1106 14-15 0.20056749941627317 1106 16-17 0.12134339952631734 1106 18-19 0.16730743895431033 1106 20-21 0.2784870296319255 1106 22-23 0.33197368746651534 1106 24-25 0.22631998361773498 1106 26-27 0.06576886597598275 1106 28-29 0.2727784713833401 1106 30-31 -0.09355695969922095 1106 32-33 -0.1828794786417447 1106 34-35 -0.0581949822121004 1106 36-37 0.033179389238284784 1106 38-39 0.04946610571812471 1106 40-41 -0.22474609294186365 1106 42-43 -0.1790544837823944 1106 44-45 0.054837970051046625 1106 46-47 -0.0362039580639717 1106 48-49 -0.36886128396754003 1106 50-51 -0.4878682037401205 1106 52-53 -0.3415228060031481 1106 54-55 -0.29170803861146766 1106 56-57 -0.3047651504007334 1106 58-59 -0.15873501737774376 1106 60-61 0.15028530991296662 1106 62-63 0.011013106491134295 1106 64-65 -0.07572986041306962 1106 66-67 -0.32536372923974 1106 68-69 0.08279217018387897 1106 70-71 0.21760151235255876 1106 72-73 0.4532054374847334 1106 74-75 0.5185838554827917 1106 76-77 0.26173700057373495 1106 78-79 -0.0023425610936769203 1106 80-81 0.19643628038396344 1106 82-83 0.12827884878917573 1106 84-85 0.1877853717146607 1106 86-87 0.30756902117371965 1106 88-89 0.39711694154818744 1106 90-91 0.6077017917196166 1106 92-93 0.5990654865250882 1106 94-95 0.34420616983874197 1106 96-97 0.6639553879274906 1106 98-99 0.7992332451608135 1106 100-101 1.5781478580148978 1107 1 0.08176889070356452 1107 2 0.05689173663370184 1107 3 0.07457792726247447 1107 4 0.06338484438128944 1107 5 -0.18023963786940556 1107 6 -0.05743890339650193 1107 7 0.005006031558167479 1107 8 -0.06295344149002347 1107 9 0.2569161291098183 1107 10-11 0.18421404303634148 1107 12-13 0.10731394634041891 1107 14-15 0.2459647747922915 1107 16-17 0.13383125946279506 1107 18-19 0.18887737884459455 1107 20-21 0.18699032385456604 1107 22-23 0.25924486367461697 1107 24-25 0.259734688187649 1107 26-27 0.3395371416932278 1107 28-29 0.3984379721314255 1107 30-31 0.28038324556628424 1107 32-33 0.22818963441178397 1107 34-35 0.31411779832180287 1107 36-37 0.41821626878260787 1107 38-39 0.10145182637855044 1107 40-41 0.37420526805768617 1107 42-43 0.42280393026595675 1107 44-45 0.2964449207295772 1107 46-47 0.47303561460578436 1107 48-49 0.4335312928757844 1107 50-51 0.30178714969977705 1107 52-53 0.25804540146724975 1107 54-55 0.3146323556452941 1107 56-57 0.5380964922482931 1107 58-59 0.560704833529158 1107 60-61 0.6591836483648166 1107 62-63 0.39883235735078415 1107 64-65 0.5106286455060314 1107 66-67 0.6177748381015178 1107 68-69 0.4942222040448172 1107 70-71 0.6677353811611297 1107 72-73 0.5253895989672728 1107 74-75 0.755715388149401 1107 76-77 0.8355617111808691 1107 78-79 0.7673046552225671 1107 80-81 0.4039591824497464 1107 82-83 0.5444402263254666 1107 84-85 0.6125825998428596 1107 86-87 0.7686420715451412 1107 88-89 0.756502194980218 1107 90-91 0.8503783804273901 1107 92-93 1.0866694973395887 1107 94-95 1.1076324238744952 1107 96-97 1.4415166132208235 1107 98-99 1.6144221051760361 1107 100-101 2.200047220169399 1108 1 0.05408970924744949 1108 2 0.07710537125517902 1108 3 0.0584303493991527 1108 4 0.008276443974686742 1108 5 -0.13490477143270851 1108 6 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-0.5320524900990122 2316 54-55 -0.3080217222045647 2316 56-57 -0.174682508369564 2316 58-59 -0.09103192118200099 2316 60-61 -0.17919872729165576 2316 62-63 -0.03883658766003606 2316 64-65 0.05144093311801612 2316 66-67 0.03710118193840373 2316 68-69 -0.08704034615598744 2316 70-71 -0.024919855168803906 2316 72-73 0.04281024308219372 2316 74-75 -0.25911798379993556 2316 76-77 -0.21665195116080227 2316 78-79 -0.17133451685034018 2316 80-81 -0.24460140605813407 2316 82-83 -0.24155471497821068 2316 84-85 -0.06175899731500323 2316 86-87 -0.5925430535707079 2316 88-89 -0.704514950524878 2316 90-91 -0.648472793499085 2316 92-93 -0.8881480420487335 2316 94-95 -0.6902803811919291 2316 96-97 -0.9532596070448562 2316 98-99 -1.3519288756704277 2316 100-101 -2.159361568832768 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 2.0 5 17.0 6 27.0 7 61.0 8 128.0 9 284.0 10 480.0 11 723.0 12 1156.0 13 1562.0 14 1867.0 15 2732.0 16 3790.0 17 5089.0 18 4716.0 19 3110.0 20 2703.0 21 2848.0 22 3102.0 23 3345.0 24 3642.0 25 4237.0 26 5112.0 27 6432.0 28 8070.0 29 10205.0 30 12881.0 31 16388.0 32 21546.0 33 28638.0 34 40529.0 35 60543.0 36 97073.0 37 143033.0 38 95855.0 39 5237.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.2404026256446 18.865537912016112 14.87530198598718 22.01875747635211 2 16.039787998291928 22.030594559292656 36.43750052330596 25.49211691910946 3 21.907345541014628 22.777624274697946 30.160843318010933 25.15418686627649 4 14.313799368683696 16.462284293285776 35.53741428248474 33.68650205554579 5 14.047619845175207 37.873453678177476 33.42215716290771 14.656769313739613 6 33.366992372292415 35.14606515787094 15.421365954133279 16.065576515703363 7 28.64149774350473 32.678405465826025 20.065476040960203 18.614620749709044 8 26.24450528748336 32.24837356509507 19.566451483258398 21.94066966416317 9 24.95524687481684 18.232816725695578 20.972093140086912 35.839843259400666 10-11 25.91997186707192 25.56755670543317 26.27690839215292 22.23556303534199 12-13 27.39882941043483 23.28560543260737 26.44544366014385 22.87012149681395 14-15 23.4474558957742 25.459881272345164 26.56208920482614 24.5305736270545 16-17 22.397494829737173 28.039905218825616 26.85614528647861 22.706454664958596 18-19 21.890348563629818 28.198906499878596 28.184756306883358 21.72598862960823 20-21 23.271245420018623 25.618171892478447 28.125188390458906 22.985394297044028 22-23 22.887096342043392 25.569106644070978 28.38391128735155 23.159885726534085 24-25 24.42992951673161 25.9539690168346 27.64647173384821 21.969629732585574 26-27 23.453310659134278 25.099450812143253 29.52318034947405 21.92405817924842 28-29 22.247443898052328 27.92801467639176 28.089029631608163 21.735511793947744 30-31 22.729175667574292 25.815691554385577 28.081827041908227 23.373305736131904 32-33 22.844105063089764 26.36239565279278 28.41794143997053 22.37555784414693 34-35 24.38153609136503 25.565714668475216 27.655338139375214 22.397411100784538 36-37 23.36952380792897 24.998974319471433 29.02064673971745 22.610855132882147 38-39 22.79370023360378 26.321619652859763 28.588078671723892 22.296601441812562 40-41 22.983848685036797 26.554553599089033 27.942109802148487 22.519487913725687 42-43 22.53402713461967 26.83885532836373 26.549287636682145 24.077829900334454 44-45 23.581505948942088 25.641908015372632 27.019919117831755 23.756666917853526 46-47 24.07919084340174 26.986846181541114 26.823490994951143 22.110471980106 48-49 24.008691065283465 26.64205035459211 26.620448284812404 22.728810295312016 50-51 22.23958202506845 27.25745815645592 28.087379534969397 22.415580283506234 52-53 23.819651034179024 27.663566739148095 25.53986380637161 22.976918420301274 54-55 22.661492217393853 26.663652424371826 26.581430592884715 24.09342476534961 56-57 23.87095693819966 26.7528237589276 26.334430182612845 23.041789120259896 58-59 25.338139375214556 26.01927440489647 25.94031800256211 22.70226821732687 60-61 23.68340408429831 26.556395636046986 27.845654048713502 21.9145462309412 62-63 22.433916924133197 26.787236358460394 27.937672167658857 22.841174549747556 64-65 22.626660973097888 28.14448268066615 26.62128557433875 22.607570771897215 66-67 23.220382976229352 26.65812631349794 26.053017172808186 24.06847353746452 68-69 22.665427478167675 26.527844063198614 26.812438773203382 23.994289685430324 70-71 24.185191697437055 26.760108177806803 26.055947686150393 22.998752438605745 72-73 23.81695176375039 25.47327790476669 27.96195356392287 22.747816767560057 74-75 22.758031699781466 28.72773856471829 25.752765148660757 22.761464586839484 76-77 23.457670827995614 26.457428014032974 26.30587860976447 23.779022548206942 78-79 23.415722622725713 26.731137960195255 26.715396917099966 23.137742499979066 80-81 23.77592457695947 26.595580785879953 26.29440774325354 23.334086893907045 82-83 23.218811566997033 26.317538969580408 27.313914340185995 23.14973512323656 84-85 22.95491690724826 27.28505067284699 26.631712561373426 23.128319858531324 86-87 22.906399403849857 27.25067611129252 26.375373640451134 23.46755084440649 88-89 22.80165448410406 27.254778829971617 25.104954242127388 24.83861244379694 90-91 23.250225041344805 26.051539701480042 26.05179091042308 24.646444346752077 92-93 23.790177737846932 27.01076039511441 26.42857202665691 22.77048984038175 94-95 23.36690864333978 26.55698173871543 26.279671447589863 23.79643817035493 96-97 21.631207455225944 27.051652390880243 27.720228077666974 23.59691207622684 98-99 22.605256195136327 27.559858128949934 25.470391619024653 24.364494056889086 100-101 21.70840856029825 27.050974573006727 26.382892132068964 24.857724734626068 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 379.0 1 256.0 2 143.5 3 158.5 4 149.0 5 158.0 6 242.0 7 428.0 8 635.5 9 715.0 10 711.0 11 696.0 12 736.5 13 803.0 14 841.5 15 896.0 16 899.5 17 893.5 18 924.0 19 938.0 20 959.5 21 1044.5 22 1189.5 23 1327.0 24 1424.5 25 1623.0 26 1913.5 27 2295.5 28 2758.5 29 3308.5 30 4041.0 31 4888.0 32 5754.5 33 6202.5 34 6918.0 35 7999.5 36 9097.0 37 10252.0 38 11536.0 39 12422.0 40 12699.5 41 14254.5 42 16500.0 43 18330.0 44 19613.0 45 20295.0 46 21490.5 47 29508.5 48 44431.0 49 43527.5 50 30768.5 51 25218.0 52 23330.5 53 23305.0 54 23291.5 55 20905.0 56 17541.5 57 14863.5 58 13359.0 59 12031.5 60 10024.5 61 7928.5 62 6196.5 63 4841.0 64 3531.0 65 2660.0 66 1970.5 67 1546.0 68 1219.0 69 943.5 70 685.5 71 453.5 72 391.0 73 330.5 74 259.0 75 195.0 76 138.5 77 91.0 78 43.5 79 31.5 80 27.5 81 16.5 82 5.5 83 2.0 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04739058719114483 2 0.0 3 0.0 4 0.0 5 0.017248164242713488 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00502373715807189 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.6745790526906298E-4 22-23 1.6745790526906298E-4 24-25 3.3491581053812596E-4 26-27 0.0017583080053251612 28-29 0.0025955975316704766 30-31 5.02373715807189E-4 32-33 0.0 34-35 0.0 36-37 8.372895263453149E-5 38-39 0.0 40-41 0.0 42-43 0.011387137558296285 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 8.372895263453149E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.372895263453149E-5 84-85 1.6745790526906298E-4 86-87 0.0 88-89 0.0 90-91 0.008791540026625808 92-93 0.003600344963284854 94-95 0.0 96-97 0.0 98-99 5.02373715807189E-4 100-101 0.013899006137332227 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 597165.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.73180895994678 #Duplication Level Percentage of deduplicated Percentage of total 1 66.50481668375447 25.09347038027705 2 15.484991128872974 11.685534540422115 3 6.509273117568032 7.368199492205825 4 3.374504455449063 5.093046297899731 5 2.039300454212837 3.8473247575145737 6 1.278703065595434 2.8948667872527127 7 0.8450271162766365 2.231908120212735 8 0.6065404666226502 1.8308695210466248 9 0.480066204565692 1.630238968691948 >10 2.553669160762437 17.783376986914327 >50 0.21510667163287464 5.504585592370902 >100 0.09545358551167818 6.042463786404032 >500 0.0044813889911586 1.270887304284671 >1k 0.0076183612849696205 6.064107423305102 >5k 4.4813889911585994E-4 1.6591200411976037 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9812 1.643096966500046 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4269 0.7148777975936299 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4005 0.6706689106025973 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2978 0.4986896418912696 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2412 0.4039084675089799 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2355 0.3943633669086433 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2248 0.3764453710448536 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2241 0.37527316570797015 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2078 0.3479775271491129 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1859 0.3113042458951881 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1858 0.311136787989919 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1621 0.2714492644411511 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1506 0.25219160533520885 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1476 0.24716786817713696 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1430 0.23946480453476007 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1310 0.2193698559024725 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1198 0.20061457051233747 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1019 0.17063960546917517 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 960 0.16075958905830048 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 960 0.16075958905830048 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 933 0.15623822561603576 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 858 0.14367888272085605 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 824 0.1379853139417079 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 671 0.11236425443554128 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 642 0.10750797518273844 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.372895263453149E-5 0.0 18-19 0.0 0.0 0.0 1.6745790526906298E-4 0.0 20-21 0.0 0.0 0.0 3.3491581053812596E-4 0.0 22-23 0.0 0.0 0.0 3.3491581053812596E-4 0.0 24-25 0.0 0.0 0.0 7.535605737107834E-4 0.0 26-27 0.0 0.0 0.0 0.0015071211474215669 0.0 28-29 0.0 0.0 0.0 0.004437634489630169 0.0 30-31 0.0 0.0 0.0 0.012056969179372535 0.0 32-33 0.0 0.0 0.0 0.02252308825868897 0.0 34-35 0.0 0.0 0.0 0.03039360980633493 0.0 36-37 0.0 0.0 0.0 0.03499870220123416 0.0 38-39 0.0 0.0 0.0 0.03985498145403699 0.0 40-41 0.0 0.0 0.0 0.04588346604372326 0.0 42-43 0.0 0.0 0.0 0.056265856170405165 0.0 44-45 0.0 0.0 0.0 0.06656451734445254 0.0 46-47 0.0 0.0 0.0 0.07803538385538336 0.0 48-49 0.0 0.0 0.0 0.08975743722421775 0.0 50-51 0.0 0.0 0.0 0.1058333961300478 0.0 52-53 0.0 0.0 0.0 0.14108328518918556 0.0 54-55 0.0 0.0 0.0 0.18612946170656353 0.0 56-57 0.0 0.0 0.0 0.24783769979821324 0.0 58-59 0.0 0.0 0.0 0.325621896795693 0.0 60-61 0.0 0.0 0.0 0.43564174055746735 0.0 62-63 0.0 0.0 0.0 0.5134259375549471 0.0 64-65 8.372895263453149E-5 0.0 0.0 0.5928847136051175 0.0 66-67 1.6745790526906298E-4 0.0 0.0 0.7543978632371288 0.0 68-69 1.6745790526906298E-4 0.0 0.0 0.958277862902213 0.0 70-71 1.6745790526906298E-4 0.0 0.0 1.1359507003926888 0.0 72-73 1.6745790526906298E-4 0.0 0.0 1.2983848685036798 0.0 74-75 1.6745790526906298E-4 0.0 0.0 1.6250952416836217 0.0 76-77 1.6745790526906298E-4 0.0 0.0 1.8545963008548725 0.0 78-79 1.6745790526906298E-4 0.0 0.0 2.0690261485519077 0.0 80-81 1.6745790526906298E-4 0.0 0.0 2.2811953145278103 0.0 82-83 1.6745790526906298E-4 0.0 0.0 2.6055612770339858 0.0 84-85 1.6745790526906298E-4 0.0 0.0 2.951110664556697 0.0 86-87 1.6745790526906298E-4 0.0 0.0 3.430124002578852 0.0 88-89 1.6745790526906298E-4 0.0 0.0 4.083544748938735 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTAAG 95 7.2759576E-12 50.017727 1 ATAGGAC 130 2.055458E-10 36.53917 3 CTATAGG 55 0.0025741584 34.5577 1 GAACGAT 70 2.0519215E-4 33.929226 6 CATAAGG 220 0.0 30.22786 2 TCGTGTC 65 0.0058389087 29.233784 9 GGACCGC 85 6.4028206E-4 27.941717 6 ATAAGGC 260 0.0 27.404379 3 GTAGCAC 140 5.797265E-7 27.143383 3 GCATAAG 295 0.0 25.771845 1 ACTCGTC 95 0.0012257256 25.000486 3 CCTATAA 370 0.0 24.400537 1 GTAAGGT 200 2.0494554E-8 23.75046 4 TAATGCC 180 1.9473919E-7 23.75046 4 TAAGGCC 285 3.6379788E-12 23.333786 4 TAATATA 105 0.0021947476 22.619486 4 GTGTTAC 190 3.27027E-7 22.507977 1 TATAAGC 365 0.0 22.123716 3 GAGTACT 2060 0.0 22.02198 12-13 TTACATA 240 5.9117156E-9 21.771255 4 >>END_MODULE