##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937851_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 79197 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.969241259138602 31.0 31.0 34.0 30.0 34.0 2 32.24610780711391 33.0 31.0 34.0 31.0 34.0 3 32.31635036680682 34.0 31.0 34.0 30.0 34.0 4 35.92304001414195 37.0 35.0 37.0 35.0 37.0 5 35.85073929568039 37.0 35.0 37.0 35.0 37.0 6 35.969405406770456 37.0 35.0 37.0 35.0 37.0 7 35.91161281361668 37.0 35.0 37.0 35.0 37.0 8 35.943899390128415 37.0 35.0 37.0 35.0 37.0 9 37.515259416392034 39.0 37.0 39.0 35.0 39.0 10-11 37.44350796116015 39.0 37.0 39.0 34.5 39.0 12-13 37.428134904099906 39.0 37.0 39.0 34.0 39.0 14-15 38.33962145030746 40.0 38.0 41.0 33.5 41.0 16-17 38.42355771051933 40.0 38.0 41.0 34.0 41.0 18-19 38.50484866851017 40.0 38.0 41.0 34.0 41.0 20-21 38.433949518289836 40.0 38.0 41.0 34.0 41.0 22-23 38.38144121620769 40.0 38.0 41.0 34.0 41.0 24-25 38.294158869654154 40.0 37.5 41.0 34.0 41.0 26-27 38.010057199136334 40.0 37.0 41.0 33.5 41.0 28-29 37.91064686793692 40.0 37.0 41.0 33.5 41.0 30-31 37.9504526686617 40.0 37.0 41.0 34.0 41.0 32-33 37.70558859552761 40.0 36.0 41.0 33.0 41.0 34-35 37.40126519943937 39.0 35.0 41.0 33.0 41.0 36-37 37.15201964720886 39.5 35.0 41.0 32.5 41.0 38-39 36.96599618672424 39.0 35.0 41.0 32.5 41.0 40-41 36.891630996123595 39.0 35.0 41.0 32.5 41.0 42-43 36.544136772857556 39.0 35.0 41.0 31.0 41.0 44-45 36.22432667904087 39.0 35.0 41.0 31.0 41.0 46-47 35.687627056580425 39.0 35.0 41.0 29.0 41.0 48-49 35.19524729472076 39.0 35.0 40.0 27.0 41.0 50-51 34.74444107731354 38.0 35.0 40.0 24.0 41.0 52-53 34.67166685606778 39.0 35.0 40.0 23.5 41.0 54-55 34.452517140800786 38.5 34.5 40.0 22.5 41.0 56-57 34.01318863088248 38.0 34.0 40.0 20.0 41.0 58-59 33.76858340593709 38.0 34.0 40.0 16.5 41.0 60-61 33.12805409295807 38.0 33.0 40.0 8.0 41.0 62-63 32.82676742805914 37.0 33.0 40.0 2.0 41.0 64-65 32.35935073298231 37.0 32.5 39.5 2.0 40.5 66-67 31.78713208833668 36.0 31.5 39.0 2.0 40.0 68-69 31.232243645592636 36.0 31.0 38.5 2.0 40.0 70-71 30.605338586057552 35.0 30.5 37.5 2.0 40.0 72-73 30.040569718549946 35.0 29.5 37.0 2.0 39.0 74-75 29.593387375784438 34.0 29.0 36.5 2.0 39.0 76-77 28.41446014369231 33.0 28.0 35.0 2.0 37.5 78-79 28.959076732704524 34.0 29.0 36.0 2.0 37.5 80-81 28.64167834640201 34.0 29.0 35.0 2.0 37.0 82-83 28.22570299380027 34.0 28.0 35.0 2.0 37.0 84-85 27.83136987512153 34.0 28.0 35.0 2.0 36.0 86-87 27.675126583077642 34.0 28.0 35.0 2.0 36.0 88-89 27.14860411379219 33.5 26.0 35.0 2.0 35.0 90-91 26.640049496824375 33.0 25.0 35.0 2.0 35.0 92-93 26.23534351048651 33.0 25.0 35.0 2.0 35.0 94-95 25.699994949303637 32.0 23.5 35.0 2.0 35.0 96-97 24.985883303660493 32.0 18.5 34.0 2.0 35.0 98-99 24.319702766518937 32.0 4.5 34.0 2.0 35.0 100-101 21.654191446645704 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3974617329825598 1101 2 0.43787773596991286 1101 3 0.26252779138291515 1101 4 0.11830622578310823 1101 5 0.37623777647054624 1101 6 0.17499884827994805 1101 7 0.483837844178737 1101 8 0.5327296804659625 1101 9 0.5283120828928105 1101 10-11 0.38992501663905443 1101 12-13 0.04542506373930166 1101 14-15 0.5069662860293818 1101 16-17 0.2122008399551376 1101 18-19 0.5198782816646315 1101 20-21 0.4622552987302697 1101 22-23 0.6180742557185397 1101 24-25 0.08006581601662788 1101 26-27 0.7066030454584435 1101 28-29 0.04204616011560347 1101 30-31 0.8934949570995059 1101 32-33 1.0069256548433358 1101 34-35 0.9257389844801764 1101 36-37 0.7621969892881779 1101 38-39 0.3614534600701802 1101 40-41 0.6315165734269002 1101 42-43 1.1997468591415483 1101 44-45 0.8019004559671785 1101 46-47 1.0274410178372975 1101 48-49 0.43922413201554633 1101 50-51 0.921776048814337 1101 52-53 0.9890260292842541 1101 54-55 0.8766791009410966 1101 56-57 0.35543236276769363 1101 58-59 0.20916988511915946 1101 60-61 0.2193840570372103 1101 62-63 0.45343359850816967 1101 64-65 0.8306954126988586 1101 66-67 1.0868775816167258 1101 68-69 0.49644918518249526 1101 70-71 0.08998389937385198 1101 72-73 0.19826658193615287 1101 74-75 0.712018328173496 1101 76-77 0.752283613183721 1101 78-79 0.1971312879167435 1101 80-81 0.24263133057817043 1101 82-83 -0.17641491291667677 1101 84-85 0.16502073444964793 1101 86-87 0.39331004930515334 1101 88-89 0.5422870409844514 1101 90-91 0.5054695066288417 1101 92-93 0.8153080913220556 1101 94-95 0.3255962029458459 1101 96-97 -0.38577321872204706 1101 98-99 -0.3034750866500744 1101 100-101 1.285167297976468 1102 1 0.3461991067199328 1102 2 0.3247464228385937 1102 3 0.26252779138291515 1102 4 0.17386178133866537 1102 5 0.18280343303620583 1102 6 0.07297864625974171 1102 7 0.1868681472090401 1102 8 0.08954786228414235 1102 9 -0.2042636746829487 1102 10-11 -0.08242346820942714 1102 12-13 -0.318842613028373 1102 14-15 -0.281417552354462 1102 16-17 -0.36620825095395304 1102 18-19 -0.123051011264657 1102 20-21 -0.6840830851081137 1102 22-23 -0.17447624933196693 1102 24-25 -0.11488367893286267 1102 26-27 0.09852223737763666 1102 28-29 0.04558151365095853 1102 30-31 -0.09400504290049128 1102 32-33 0.16740545282312524 1102 34-35 0.26071373195492953 1102 36-37 0.011818201409390383 1102 38-39 0.4074130560297746 1102 40-41 0.667880209790539 1102 42-43 0.34873675813144445 1102 44-45 0.9885166175833362 1102 46-47 0.7223905127867951 1102 48-49 0.12028473807615114 1102 50-51 0.41356897810727133 1102 52-53 -0.19544366768543853 1102 54-55 -0.4061491818871943 1102 56-57 -0.1573201624848366 1102 58-59 -0.3110321350828613 1102 60-61 -0.2943785692254224 1102 62-63 0.015554810629382132 1102 64-65 0.49950854401198796 1102 66-67 0.30619576343490706 1102 68-69 0.19202999326330428 1102 70-71 0.31801420240415723 1102 72-73 0.46594334961292105 1102 74-75 0.43979610595127383 1102 76-77 0.36086947176957906 1102 78-79 0.41013633842179686 1102 80-81 0.6149798154266577 1102 82-83 0.8667669052651448 1102 84-85 0.3148944718233828 1102 86-87 0.44141610991121283 1102 88-89 1.3542819904794001 1102 90-91 0.8193583955177317 1102 92-93 0.7134141519281165 1102 94-95 0.041252768602412004 1102 96-97 0.18607021562138826 1102 98-99 1.1096562264812384 1102 100-101 1.3435006313098015 1103 1 0.5176098432992084 1103 2 0.35258662258890894 1103 3 0.40297722958516147 1103 4 -0.02776118994722765 1103 5 0.28405187248689145 1103 6 0.3312807686068098 1103 7 0.10896552548619098 1103 8 0.3816826937448141 1103 9 0.405099621197202 1103 10-11 0.14547915351342056 1103 12-13 0.024166126047781233 1103 14-15 0.21227782841958032 1103 16-17 0.39165691758537236 1103 18-19 0.2453634706329666 1103 20-21 0.5371403855535561 1103 22-23 0.6246498430525591 1103 24-25 0.5994733747500263 1103 26-27 0.1435284795748899 1103 28-29 0.5606876310042566 1103 30-31 0.3464443953017522 1103 32-33 0.31559521561963066 1103 34-35 0.41814194668651083 1103 36-37 0.8209942313719409 1103 38-39 0.5953656153306568 1103 40-41 0.9925993109141373 1103 42-43 -0.07604476496468493 1103 44-45 0.4496277286944448 1103 46-47 -0.20264069819947395 1103 48-49 -0.3793407300886429 1103 50-51 -0.10484550379036506 1103 52-53 0.3508110139624989 1103 54-55 -0.3590205926237715 1103 56-57 -0.4487683522476331 1103 58-59 -0.23643787790432924 1103 60-61 0.09444789769093376 1103 62-63 0.319112863063836 1103 64-65 0.652005422913362 1103 66-67 0.19215081961468172 1103 68-69 1.0700574589312168 1103 70-71 1.2979767492206378 1103 72-73 1.0470919139075505 1103 74-75 1.6558385528926003 1103 76-77 0.7762876989855592 1103 78-79 0.3160664258125543 1103 80-81 0.09943674426560989 1103 82-83 0.005530950208964214 1103 84-85 0.347541163458839 1103 86-87 -0.048658796455828934 1103 88-89 -0.14827731039450853 1103 90-91 0.30462680999962544 1103 92-93 0.2385077848869166 1103 94-95 0.34474839906433274 1103 96-97 0.8640352593167684 1103 98-99 0.9475463638095754 1103 100-101 1.5815156125832068 1104 1 0.19529471395507514 1104 2 -0.06000809912522698 1104 3 0.44857430301082246 1104 4 0.374120179271479 1104 5 0.2879713916925368 1104 6 0.2585083620220203 1104 7 0.538806131705158 1104 8 0.48489669949344716 1104 9 0.1903099687279024 1104 10-11 0.0761036635735195 1104 12-13 0.606867981286868 1104 14-15 0.27387960526828437 1104 16-17 0.4977452374181368 1104 18-19 0.4601531231022662 1104 20-21 0.3654001190262548 1104 22-23 0.040510830771395945 1104 24-25 -0.006614945082738188 1104 26-27 0.255757379496508 1104 28-29 0.6197417203692979 1104 30-31 0.21762286407624742 1104 32-33 -0.3645066918797113 1104 34-35 -0.41318807683060044 1104 36-37 -0.3885693954898315 1104 38-39 -0.6401321636084631 1104 40-41 -1.0170035111396984 1104 42-43 -1.5201262909641642 1104 44-45 -1.6201397131660187 1104 46-47 -2.0486689187377536 1104 48-49 -2.3490951068850876 1104 50-51 -2.901030505096866 1104 52-53 -2.5969940552823374 1104 54-55 -3.0889657193548956 1104 56-57 -2.7823847619680393 1104 58-59 -2.3510838146694226 1104 60-61 -1.5976085433856255 1104 62-63 -2.0243676699907738 1104 64-65 -2.287829957280003 1104 66-67 -1.4042693528441639 1104 68-69 -1.6268330558323072 1104 70-71 -1.183536185192743 1104 72-73 -1.4248835237720883 1104 74-75 -1.956586322989292 1104 76-77 -1.7392597271968278 1104 78-79 -1.7815949277280758 1104 80-81 -2.333082200077218 1104 82-83 -2.0006749551999725 1104 84-85 -1.6662424790810064 1104 86-87 -1.8023823397011896 1104 88-89 -1.9935216270916563 1104 90-91 -1.375344446859529 1104 92-93 -1.2001517395447543 1104 94-95 -1.4291606680900983 1104 96-97 -0.966772161639593 1104 98-99 -1.1776822748107527 1104 100-101 -0.9328559578374858 1105 1 0.0451890057098332 1105 2 -0.2212131731209972 1105 3 0.47843688229200865 1105 4 0.2660334985103816 1105 5 0.10351050374327286 1105 6 -0.2113647880836922 1105 7 0.1429287532696435 1105 8 -0.17181577407949078 1105 9 0.26694844652917027 1105 10-11 0.15128865300269467 1105 12-13 0.02837960919384841 1105 14-15 0.16605719512029538 1105 16-17 0.15538265813695062 1105 18-19 0.14487828166463856 1105 20-21 -0.6002447012697374 1105 22-23 -0.22851665337237392 1105 24-25 0.03461127056208113 1105 26-27 -0.03771513635972923 1105 28-29 -0.617044748975303 1105 30-31 -0.6633232247186811 1105 32-33 -0.6862561633384843 1105 34-35 -0.011761015519816453 1105 36-37 0.2962878983790844 1105 38-39 -0.036273812657093174 1105 40-41 0.07469839160872027 1105 42-43 0.08042867732336134 1105 44-45 0.05190045596717141 1105 46-47 -0.2736953457990694 1105 48-49 -0.2312304134389933 1105 50-51 0.3535942306325168 1105 52-53 0.15948057473880084 1105 54-55 -0.5437754445134502 1105 56-57 -0.6843403645050401 1105 58-59 -0.26242102397174705 1105 60-61 -0.5192523065991566 1105 62-63 -0.8136118560372836 1105 64-65 -0.4818045873011414 1105 66-67 -0.771076963837821 1105 68-69 -0.9069599057265947 1105 70-71 -1.660016100626148 1105 72-73 -0.9096879635183939 1105 74-75 -1.5834362172810508 1105 76-77 -0.8840800231799157 1105 78-79 -1.6153687120832565 1105 80-81 -2.0585050330581893 1105 82-83 -1.8298240038257632 1105 84-85 -1.6758883564594456 1105 86-87 -2.7203263143312135 1105 88-89 -2.230440231742822 1105 90-91 -1.5570304933711583 1105 92-93 -1.6733282723143112 1105 94-95 -1.5778128879632476 1105 96-97 -1.573273218722047 1105 98-99 -1.502338723013711 1105 100-101 -1.368241792932622 1106 1 0.3325790350353479 1106 2 0.2179363869962998 1106 3 0.11736650106033153 1106 4 0.29572558062182 1106 5 0.0104019993444453 1106 6 0.10021878962891861 1106 7 0.10407244828430606 1106 8 -0.02335536352230605 1106 9 0.19143525004823658 1106 10-11 -0.13995034992985467 1106 12-13 -0.03210426177389536 1106 14-15 0.4329193652082708 1106 16-17 -0.001692121921699652 1106 18-19 -0.4280689324116125 1106 20-21 0.8044473808417081 1106 22-23 0.2678176574780693 1106 24-25 0.1944353174829061 1106 26-27 0.08490216569305176 1106 28-29 0.13662094017425375 1106 30-31 0.35922076355112154 1106 32-33 0.6801294671600502 1106 34-35 0.40282842729543233 1106 36-37 0.9628934702265965 1106 38-39 1.2764094718003847 1106 40-41 0.4614285968873162 1106 42-43 0.25913102336442506 1106 44-45 0.0383374061137971 1106 46-47 -0.19176719330639713 1106 48-49 -0.8485324662249241 1106 50-51 -0.5685098749393305 1106 52-53 0.7362767624220865 1106 54-55 0.9558579865715942 1106 56-57 1.0880203393072847 1106 58-59 1.5984660728024451 1106 60-61 1.4145820042512796 1106 62-63 0.3617913697691648 1106 64-65 0.2579314830800854 1106 66-67 0.7142602795639377 1106 68-69 1.4991984520446664 1106 70-71 1.1577991486407129 1106 72-73 0.7114630628745715 1106 74-75 0.720082844302528 1106 76-77 0.4612278947086459 1106 78-79 0.0968746896762731 1106 80-81 -0.18717072220775322 1106 82-83 0.2506378730070793 1106 84-85 0.006113109816212159 1106 86-87 0.7919537443198159 1106 88-89 0.15464041341846624 1106 90-91 -0.8695304933711583 1106 92-93 -1.5521772459213459 1106 94-95 -1.3892131812183806 1106 96-97 -1.338302544235244 1106 98-99 -1.0270821247732407 1106 100-101 -0.8317681858945001 1107 1 0.40643302484859234 1107 2 0.12328443453450433 1107 3 0.4993698966460727 1107 4 0.1972535942041631 1107 5 -0.0914655728117495 1107 6 -0.3106470847344056 1107 7 -0.2482195720892122 1107 8 0.08954786228414235 1107 9 0.3781924656679294 1107 10-11 0.16055898793092638 1107 12-13 0.4033796091938484 1107 14-15 0.19416724296718257 1107 16-17 0.46668648588815387 1107 18-19 0.6209548366885542 1107 20-21 0.5086069733714211 1107 22-23 0.3085646863405529 1107 24-25 0.8270753853946147 1107 26-27 0.5929666818220838 1107 28-29 0.34587391131177014 1107 30-31 0.015205483415293486 1107 32-33 0.017990248144769794 1107 34-35 0.15091841031750164 1107 36-37 -0.16932214946780277 1107 38-39 0.40989843614674015 1107 40-41 0.7810381045273829 1107 42-43 1.3779765242133166 1107 44-45 1.3575224655365545 1107 46-47 1.017273553722461 1107 48-49 0.7645829836901896 1107 50-51 0.4735104985751022 1107 52-53 0.37867913933210673 1107 54-55 0.9586168999841576 1107 56-57 0.6236739895619436 1107 58-59 1.0698158181335131 1107 60-61 1.2776974541663932 1107 62-63 1.6567828808048262 1107 64-65 1.5654442165266076 1107 66-67 1.3575115529085906 1107 68-69 1.1067960751346533 1107 70-71 1.417137009421701 1107 72-73 1.5003000747591209 1107 74-75 1.1528078018577048 1107 76-77 0.5531209337578851 1107 78-79 0.7955164553808594 1107 80-81 1.0969973592863056 1107 82-83 1.174661642107246 1107 84-85 1.5080231267941429 1107 86-87 0.9001880397357773 1107 88-89 1.4965334524677054 1107 90-91 1.3607326645235815 1107 92-93 0.6182387133316247 1107 94-95 -0.12775307935080704 1107 96-97 0.7550760635746023 1107 98-99 1.4190129516274368 1107 100-101 1.6009567716606803 1108 1 0.23350069402152585 1108 2 0.13268293077510407 1108 3 0.2228452517003774 1108 4 0.24529035276723476 1108 5 -0.4156092653764958 1108 6 0.02059769387879129 1108 7 -0.21313185279096558 1108 8 0.010182782919059719 1108 9 -0.26299383341310545 1108 10-11 -0.08797902376498001 1108 12-13 0.2724272282414688 1108 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-1.6124432561709447 2316 74-75 -1.6783926307306167 2316 76-77 -1.0017637093567515 2316 78-79 -1.724490754424476 2316 80-81 -1.8099060293221996 2316 82-83 -0.9969317248718426 2316 84-85 -1.2070994398965524 2316 86-87 -1.5857528398604757 2316 88-89 -1.0754744783181636 2316 90-91 -1.3078866577547217 2316 92-93 -0.7664945238709713 2316 94-95 -1.5725980685360987 2316 96-97 -0.5569282623085989 2316 98-99 -0.6817440779327661 2316 100-101 -0.8809285924344898 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 5.0 6 5.0 7 24.0 8 43.0 9 62.0 10 127.0 11 180.0 12 331.0 13 430.0 14 518.0 15 750.0 16 1014.0 17 1372.0 18 1214.0 19 767.0 20 784.0 21 763.0 22 773.0 23 826.0 24 823.0 25 835.0 26 834.0 27 1035.0 28 1269.0 29 1505.0 30 1888.0 31 2252.0 32 2835.0 33 3746.0 34 5208.0 35 7815.0 36 11254.0 37 15150.0 38 11878.0 39 882.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.9318549201859 23.9164477672257 16.38588603758335 15.765811275005051 2 13.869212217634505 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30.697501167973535 20.69144033233582 46-47 21.758399939391644 32.800484866851015 30.760003535487456 14.681111658269884 48-49 20.474260388651086 31.370506458577978 30.76631690594341 17.38891624682753 50-51 14.876826142404385 32.806798237306964 34.67239920704067 17.643976413247977 52-53 19.185070141545765 34.7210121595515 28.166470952182532 17.927446746720204 54-55 17.301269626814317 31.27410933563983 29.959025967056625 21.465595070489226 56-57 20.21541219995707 30.409611475182142 29.693675265477225 19.681301059383564 58-59 23.506572218644646 29.39063348359155 28.785812593911388 18.31698170385242 60-61 18.700834627574277 33.20959127239668 32.04161773804564 16.04795636198341 62-63 16.85291109511724 31.848428602093513 32.61108375317247 18.68757654961678 64-65 16.68181875576095 35.88646034572017 29.39884086518429 18.032880033334596 66-67 19.246941172014093 30.741694761165196 27.548391984544867 22.462972082275844 68-69 16.873113880576284 30.99044155712969 29.8022652373196 22.334179324974432 70-71 21.854994507367703 31.129967044206218 28.139323459221938 18.875714989204138 72-73 20.133969721075292 27.84133237370102 33.09847595237193 18.926221952851748 74-75 16.987493134166282 35.89615702714135 27.975352446130824 19.14099739256154 76-77 18.995037690821622 29.179766910362765 29.40262888745786 22.422566511357754 78-79 18.56320315163453 31.17542331148907 29.94999810598886 20.311375430887534 80-81 21.189565261310403 29.974620250767074 28.66017652183795 20.175637966084576 82-83 19.177857327752207 28.23519013075238 32.542884381057 20.044068160438407 84-85 17.647133098475955 31.14322512216372 30.514413424750934 20.69522835460939 86-87 17.97732237332222 31.455736959733322 28.837582231650188 21.729358435294266 88-89 17.379446191143604 31.562432920438905 26.749119284821393 24.309001603596094 90-91 19.679997979466325 27.0176922979782 28.615176733554748 24.68713298900072 92-93 21.368908194382186 31.16946252580672 28.341614632329264 19.120014647481835 94-95 20.10429687993232 28.898190588027322 28.962586966678032 22.03492556536232 96-97 15.701352323951664 30.232837102415495 31.938709799613623 22.12710077401922 98-99 19.233173183158346 32.01404102454023 26.359119405529285 22.393666386772143 100-101 17.310971066291156 29.348581099001024 28.008613177403667 25.331834657304153 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 634.0 1 394.5 2 147.5 3 142.5 4 133.5 5 134.0 6 194.0 7 300.5 8 342.0 9 327.0 10 312.0 11 267.0 12 270.0 13 298.0 14 270.5 15 256.5 16 250.5 17 233.5 18 250.0 19 248.5 20 270.0 21 312.5 22 331.5 23 350.5 24 373.0 25 430.5 26 531.5 27 683.0 28 775.0 29 923.5 30 1212.0 31 1438.5 32 1848.0 33 2360.0 34 2700.5 35 3078.0 36 3596.5 37 3870.0 38 3621.5 39 3226.0 40 2906.0 41 2469.5 42 1957.5 43 1858.5 44 1917.5 45 1735.0 46 1747.5 47 4033.5 48 8930.5 49 8286.0 50 3237.0 51 1138.0 52 582.5 53 338.5 54 188.5 55 158.0 56 130.0 57 96.5 58 81.5 59 76.5 60 69.5 61 56.5 62 44.0 63 33.5 64 23.5 65 12.5 66 13.0 67 13.5 68 10.0 69 9.5 70 7.0 71 2.5 72 0.5 73 1.0 74 2.5 75 2.5 76 1.0 77 1.0 78 2.0 79 1.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016414763185474197 2 0.0 3 0.0 4 0.0 5 0.04040557091809033 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0025253481823806455 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.313370455951614E-4 24-25 0.0 26-27 0.0012626740911903227 28-29 0.0018940111367854843 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.00883871863833226 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 6.313370455951614E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 6.313370455951614E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0018940111367854843 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012626740911903227 92-93 0.003156685227975807 94-95 0.0 96-97 0.0 98-99 6.313370455951614E-4 100-101 0.020202785459045164 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 79197.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.809096304152934 #Duplication Level Percentage of deduplicated Percentage of total 1 70.70930670685757 18.956526131040317 2 10.691409193669932 5.732540374004066 3 4.8605877920120575 3.90923898632524 4 2.7034664657121326 2.8990997133729812 5 2.067633760361718 2.7715696301627584 6 1.3423134890730972 2.1591726959354522 7 1.1397889977392615 2.138969910476407 8 0.8289374529012812 1.7778451203959746 9 0.6311228334589299 1.5227849539755294 >10 4.559155990957046 22.521055090470597 >50 0.273172569706104 4.902963496092023 >100 0.1177467972871138 7.603823377148124 >500 0.04709871891484552 9.632940641690974 >1k 0.02825923134890731 13.471469878909554 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4229 5.339848731643875 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1424 1.7980479058550196 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1407 1.7765824463047843 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1385 1.7488036162985972 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1180 1.489955427604581 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1044 1.318231751202697 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 998 1.260148743007942 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 959 1.2109044534515196 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 950 1.1995403866308068 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 784 0.9899364874932131 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 736 0.9293281311160776 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 696 0.8788211674684647 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 665 0.8396782706415646 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 658 0.8308395520032325 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 629 0.7942220033587131 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 554 0.6995214465194388 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 468 0.5909314746770711 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 465 0.5871434524035001 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 453 0.5719913633092163 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 403 0.5088576587497001 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 391 0.49370556965541623 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 337 0.4255211687311388 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 318 0.4015303609985227 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 311 0.39269164236019044 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 276 0.3484980491685291 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 234 0.29546573733853554 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 228 0.2878896927913936 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 224 0.2828389964266323 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 201 0.2537974923292549 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 199 0.25127214414687427 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 193 0.2436960995997323 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 188 0.2373827291437807 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 161 0.203290528681642 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 161 0.203290528681642 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 143 0.18056239504021615 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 128 0.1616222836723613 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 116 0.14647019457807745 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 112 0.14141949821331615 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 106 0.13384345366617423 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 104 0.13131810548379358 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 102 0.12879275730141293 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 96 0.121216712754271 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 95 0.11995403866308069 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 92 0.1161660163895097 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 90 0.11364066820712906 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 86 0.10858997184236777 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 86 0.10858997184236777 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 85 0.10732729775117743 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 85 0.10732729775117743 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 84 0.10606462365998712 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 83 0.10480194956879679 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 82 0.10353927547760648 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0031566852279758073 0.0 26-27 0.0 0.0 0.0 0.009470055683927421 0.0 28-29 0.0 0.0 0.0 0.015783426139879035 0.0 30-31 0.0 0.0 0.0 0.030935515234162908 0.0 32-33 0.0 0.0 0.0 0.05682033410356453 0.0 34-35 0.0 0.0 0.0 0.08144247888177583 0.0 36-37 0.0 0.0 0.0 0.10227660138641614 0.0 38-39 0.0 0.0 0.0 0.12563607207343713 0.0 40-41 0.0 0.0 0.0 0.1584655984443855 0.0 42-43 0.0 0.0 0.0 0.21212924731997423 0.0 44-45 0.0 0.0 0.0 0.29294038915615495 0.0 46-47 0.0 0.0 0.0 0.37438286803793075 0.0 48-49 0.0 0.0 0.0 0.5208530626160082 0.0 50-51 0.0 0.0 0.0 0.676793312878013 0.0 52-53 0.0 0.0 0.0 0.828945540866447 0.0 54-55 0.0 0.0 0.0 1.0372867659128502 0.0 56-57 0.0 0.0 0.0 1.2544667095975859 0.0 58-59 0.0 0.0 0.0 1.4609139235072035 0.0 60-61 0.0 0.0 0.0 1.6995593267421745 0.0 62-63 0.0 0.0 0.0 2.018384534767731 0.0 64-65 0.0 0.0 0.0 2.2759700493705566 0.0 66-67 0.0 0.0 0.0 2.620680076265515 0.0 68-69 0.0 0.0 0.0 3.0866068159147444 0.0 70-71 0.0 0.0 0.0 3.406694698031491 0.0 72-73 0.0 0.0 0.0 3.83347854085382 0.0 74-75 0.0 0.0 0.0 4.505221157367072 0.0 76-77 0.0 0.0 0.0 4.960415167241184 0.0 78-79 0.0 0.0 0.0 5.489475611449929 0.0 80-81 0.0 0.0 0.0 6.131545386820208 0.0 82-83 0.0 0.0 0.0 6.70795610944859 0.0 84-85 0.0 0.0 0.0 7.440307082338977 0.0 86-87 0.0 0.0 0.0 8.269252623205425 0.0 88-89 0.0 0.0 0.0 9.405659305276714 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACTTGC 40 5.357644E-4 47.499996 7 GTAAGCG 20 5.374027E-4 47.499996 94-95 CATGGGC 50 0.0016067399 38.0 4 ACTTGCT 50 0.0016067399 38.0 8 GTTTAGA 25 0.0016116095 38.0 40-41 CTTGCTA 50 0.0016067399 38.0 9 GTAAGTT 25 0.0016116095 38.0 36-37 CAATCAC 25 0.0016116095 38.0 28-29 AGTAAGT 25 0.0016116095 38.0 36-37 ATGGGAT 65 1.3170418E-4 36.538464 5 AGGGTGA 65 1.3170418E-4 36.538464 8 GAGGGTG 65 1.3170418E-4 36.538464 7 GGAGGGT 65 1.3170418E-4 36.538464 6 AAACTTG 55 0.0025652002 34.545456 6 GACAATG 55 0.0025652002 34.545456 7 GGACAAT 55 0.0025652002 34.545456 6 TTTAGAT 30 0.0039407583 31.666668 42-43 AGCCAAA 30 0.0039407583 31.666668 52-53 CAATGGG 60 0.003928957 31.666668 9 TCTGGAA 60 0.003928957 31.666668 3 >>END_MODULE