##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937850_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 77223 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02802273933931 31.0 31.0 34.0 30.0 34.0 2 32.22493298628647 33.0 31.0 34.0 30.0 34.0 3 32.29931497092835 34.0 31.0 34.0 30.0 34.0 4 35.90194631133212 37.0 35.0 37.0 35.0 37.0 5 35.83157867474716 37.0 35.0 37.0 35.0 37.0 6 35.934695621770715 37.0 35.0 37.0 35.0 37.0 7 35.88314362301387 37.0 35.0 37.0 35.0 37.0 8 35.91037644225166 37.0 35.0 37.0 35.0 37.0 9 37.495176307576756 39.0 37.0 39.0 35.0 39.0 10-11 37.43147766857024 39.0 37.0 39.0 34.5 39.0 12-13 37.41979073592064 39.0 37.0 39.0 34.0 39.0 14-15 38.323666524222055 40.0 37.5 41.0 33.5 41.0 16-17 38.412072828043456 40.0 38.0 41.0 33.5 41.0 18-19 38.50898048508864 40.0 38.0 41.0 34.0 41.0 20-21 38.42969711096435 40.0 38.0 41.0 34.0 41.0 22-23 38.27563679214742 40.0 37.5 41.0 34.0 41.0 24-25 38.26012327933388 40.0 37.5 41.0 34.0 41.0 26-27 37.96374137238905 40.0 37.0 41.0 33.5 41.0 28-29 37.902159978244825 40.0 37.0 41.0 33.5 41.0 30-31 37.90940522901208 40.0 37.0 41.0 33.0 41.0 32-33 37.6643616539114 40.0 36.0 41.0 33.0 41.0 34-35 37.362599225619306 39.0 35.0 41.0 33.0 41.0 36-37 37.096337878611294 39.5 35.0 41.0 32.5 41.0 38-39 36.89614493091436 39.0 35.0 41.0 32.5 41.0 40-41 36.8159550910998 39.0 35.0 41.0 32.5 41.0 42-43 36.440134415912354 39.0 35.0 41.0 31.0 41.0 44-45 36.07619491602243 39.0 35.0 41.0 30.0 41.0 46-47 35.521852297890526 39.0 35.0 41.0 28.0 41.0 48-49 35.04051901635523 39.0 35.0 40.0 26.0 41.0 50-51 34.588056667055156 38.0 35.0 40.0 23.0 41.0 52-53 34.493408699480725 38.0 35.0 40.0 23.0 41.0 54-55 34.273726739442914 38.5 34.5 40.0 21.0 41.0 56-57 33.81025730676094 38.0 34.0 40.0 16.0 41.0 58-59 33.53860896365073 38.0 34.0 40.0 10.0 41.0 60-61 32.88910687230488 38.0 33.0 40.0 3.5 41.0 62-63 32.60205508721495 37.0 33.0 40.0 2.0 41.0 64-65 32.16413503748883 36.5 32.0 39.5 2.0 40.5 66-67 31.577127280732423 36.0 31.0 39.0 2.0 40.0 68-69 31.037093870996983 35.5 31.0 38.5 2.0 40.0 70-71 30.38795436592725 35.0 30.0 37.5 2.0 39.5 72-73 29.79475674345726 34.5 29.5 37.0 2.0 39.0 74-75 29.34449581083356 34.0 29.0 36.5 2.0 39.0 76-77 28.1797391968714 33.0 27.5 35.0 2.0 37.5 78-79 28.705061963404685 34.0 29.0 36.0 2.0 37.5 80-81 28.390136358338836 34.0 29.0 35.0 2.0 37.0 82-83 27.988202996516584 34.0 27.5 35.0 2.0 36.5 84-85 27.571131657666758 34.0 27.0 35.0 2.0 36.0 86-87 27.363013609934864 34.0 26.5 35.0 2.0 36.0 88-89 26.904821102521268 33.5 25.5 35.0 2.0 35.5 90-91 26.409916734651596 33.0 25.0 35.0 2.0 35.0 92-93 25.912901596674565 33.0 24.0 35.0 2.0 35.0 94-95 25.426058298693395 32.0 20.0 35.0 2.0 35.0 96-97 24.74730973932637 32.0 16.5 34.0 2.0 35.0 98-99 24.072866891988138 32.0 2.0 34.0 2.0 35.0 100-101 21.524649392020514 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.634545265520746 1101 2 0.570369780304155 1101 3 0.41848457421374974 1101 4 0.3391290683495001 1101 5 0.6163987506750672 1101 6 0.48492539550877467 1101 7 0.42864065257591477 1101 8 0.4504683401378955 1101 9 0.4319671857602714 1101 10-11 0.6839658462276148 1101 12-13 0.47079189327185844 1101 14-15 0.6393623427115571 1101 16-17 0.42693311939621736 1101 18-19 0.9018760998855626 1101 20-21 1.072868614524971 1101 22-23 0.9739853507477179 1101 24-25 0.9496272533803847 1101 26-27 0.7017094807107469 1101 28-29 0.8594925466473384 1101 30-31 0.7245987389097337 1101 32-33 0.08734613749039255 1101 34-35 0.47391858445422486 1101 36-37 0.880577886842481 1101 38-39 0.2775452892941459 1101 40-41 0.14649874517662909 1101 42-43 0.46704740879275164 1101 44-45 0.32472817220082106 1101 46-47 -0.8520612325178263 1101 48-49 -1.25981567880563 1101 50-51 -0.9527950887233771 1101 52-53 -0.915785168966579 1101 54-55 -0.6137328645177575 1101 56-57 -0.5502546284410101 1101 58-59 -0.5295991417362558 1101 60-61 -0.873344745314526 1101 62-63 -1.3281676476655164 1101 64-65 -0.9173101346905668 1101 66-67 -0.37425221910731565 1101 68-69 -0.07825320295242832 1101 70-71 0.035369652322923884 1101 72-73 0.37438982225519624 1101 74-75 0.24963032184397704 1101 76-77 0.42009895607095515 1101 78-79 0.4666650505344947 1101 80-81 0.273389276800593 1101 82-83 0.5478366290754977 1101 84-85 0.36039227174426713 1101 86-87 0.32745811094664745 1101 88-89 0.9671102943826462 1101 90-91 1.0575983598411405 1101 92-93 1.1235268718980436 1101 94-95 0.5176831458456235 1101 96-97 1.1453424714183598 1101 98-99 1.8810817988080828 1101 100-101 2.3492930079216627 1102 1 0.13597861478018203 1102 2 0.30352792650577953 1102 3 0.36007559189172866 1102 4 0.3522681032276651 1102 5 -0.18102122065794646 1102 6 0.12611029756323688 1102 7 0.2886502082376481 1102 8 -0.05156223797964543 1102 9 0.18877559474259442 1102 10-11 0.3660012021760153 1102 12-13 -0.27263858928905194 1102 14-15 0.10520085202833229 1102 16-17 -0.11427567181257103 1102 18-19 0.3729105002677855 1102 20-21 -0.23052364538902737 1102 22-23 -0.15585220300288682 1102 24-25 -0.07067852779496064 1102 26-27 -0.11046204341730004 1102 28-29 0.26274147163538686 1102 30-31 0.13740332562736768 1102 32-33 0.5785071503905286 1102 34-35 -0.2983580519528459 1102 36-37 -0.20279526174805795 1102 38-39 0.2404573772062335 1102 40-41 -0.7722184072361742 1102 42-43 -0.44671274409783734 1102 44-45 0.41084857353860826 1102 46-47 -0.10898908727177314 1102 48-49 0.19348102449107074 1102 50-51 0.43223835609267525 1102 52-53 0.9791622748939091 1102 54-55 1.4745375607091944 1102 56-57 0.9016087255962546 1102 58-59 1.3044309585981892 1102 60-61 0.14636380700272866 1102 62-63 0.6451959452632892 1102 64-65 1.2257858997098197 1102 66-67 0.7355781678969855 1102 68-69 1.3631586460681255 1102 70-71 1.6579807368905328 1102 72-73 0.5491987090206081 1102 74-75 0.7542289840513376 1102 76-77 1.1493153918091288 1102 78-79 1.3775585049062116 1102 80-81 0.9480189949085727 1102 82-83 1.2485652482823753 1102 84-85 1.719446261233042 1102 86-87 1.7433085648405786 1102 88-89 2.01184273585422 1102 90-91 1.927521914547306 1102 92-93 1.8853520032883928 1102 94-95 1.5964576322288089 1102 96-97 2.0146090743806155 1102 98-99 2.1970158647421485 1102 100-101 1.981937537305317 1103 1 0.4423127301030654 1103 2 0.22376499281025986 1103 3 0.2617918531585488 1103 4 0.18842071466117005 1103 5 0.3276835577097543 1103 6 0.06968357821516946 1103 7 0.13406322218998667 1103 8 0.33749813984478294 1103 9 0.4050985975824517 1103 10-11 0.4221192414401216 1103 12-13 0.44880850294455144 1103 14-15 0.3547995679191729 1103 16-17 0.42974210816026215 1103 18-19 0.2849366763389085 1103 20-21 0.3107777498449522 1103 22-23 0.2330229667711663 1103 24-25 0.5558803066290423 1103 26-27 0.7441496370370757 1103 28-29 0.5795707098129483 1103 30-31 0.7829768532233672 1103 32-33 0.745626547847003 1103 34-35 0.4421037334527682 1103 36-37 0.4787703636377927 1103 38-39 -0.07217381182945104 1103 40-41 -0.003049374022197071 1103 42-43 0.4338280634092584 1103 44-45 0.25743457181000196 1103 46-47 0.2362387186301973 1103 48-49 0.3216449953516758 1103 50-51 0.22264457908317326 1103 52-53 0.2956828329875023 1103 54-55 0.6457932712907422 1103 56-57 0.925685772145215 1103 58-59 0.4388302671548061 1103 60-61 0.8837387915687174 1103 62-63 0.9103521373857717 1103 64-65 0.9710630944252188 1103 66-67 0.9120814399058723 1103 68-69 -0.3085292166309799 1103 70-71 0.546300282513446 1103 72-73 0.6016125872771809 1103 74-75 0.6513767800755375 1103 76-77 0.7187066746835562 1103 78-79 0.2495180060791924 1103 80-81 0.12365796268237261 1103 82-83 -0.19599580863823007 1103 84-85 -0.24048706386882301 1103 86-87 0.5412465818797187 1103 88-89 0.8009525024920734 1103 90-91 1.2260155557375754 1103 92-93 1.2300241850392268 1103 94-95 0.5739239860996577 1103 96-97 0.3117445232014546 1103 98-99 -0.3183564034391111 1103 100-101 0.3966183620985042 1104 1 0.4511693064414608 1104 2 0.4176843583092662 1104 3 0.2160549066945734 1104 4 0.04935924737763031 1104 5 0.36985143609706483 1104 6 -0.1452536326242182 1104 7 0.23835932265264148 1104 8 0.15878036059825007 1104 9 0.5859313801336725 1104 10-11 0.24816021962914903 1104 12-13 0.19943639455063789 1104 14-15 0.2951168184148827 1104 16-17 0.4239919429256318 1104 18-19 0.4965691945147199 1104 20-21 0.9639172078753546 1104 22-23 0.5186145067579488 1104 24-25 0.326865105042792 1104 26-27 0.0034358234217393147 1104 28-29 0.09913449038135269 1104 30-31 0.7946754652524461 1104 32-33 0.6916939635773431 1104 34-35 0.7981513210271203 1104 36-37 0.5381226438757309 1104 38-39 0.9054864657647315 1104 40-41 0.7085831441536143 1104 42-43 1.048633086542111 1104 44-45 0.4465951798734551 1104 46-47 0.9401382559732241 1104 48-49 1.460772556488486 1104 50-51 1.4797496683098714 1104 52-53 1.5204680279643696 1104 54-55 0.5830702045359573 1104 56-57 0.7537095659323896 1104 58-59 1.1913087866524918 1104 60-61 1.4214122879335633 1104 62-63 1.248686572283333 1104 64-65 1.4727154407569998 1104 66-67 0.9547119752660898 1104 68-69 0.6473861832368328 1104 70-71 0.6483491919648721 1104 72-73 0.9550164207206713 1104 74-75 0.9840932374450055 1104 76-77 1.2672856568382187 1104 78-79 1.5930077615319362 1104 80-81 1.735026105445094 1104 82-83 1.9462381636023522 1104 84-85 1.345302757677775 1104 86-87 1.385066806598818 1104 88-89 1.890575767528425 1104 90-91 1.6122019404038035 1104 92-93 1.4739105036115987 1104 94-95 1.796263708505471 1104 96-97 1.595022778323731 1104 98-99 2.107552387043377 1104 100-101 3.237464363420383 1105 1 -0.030372275708639762 1105 2 0.16332330354253344 1105 3 -0.0594014827577638 1105 4 -0.017442645793430245 1105 5 0.13563913048516696 1105 6 -0.15025701328682572 1105 7 0.27121936322059526 1105 8 0.5260555465346926 1105 9 0.21757438216206992 1105 10-11 0.04403581124510936 1105 12-13 0.50802327757971 1105 14-15 0.6216212132762706 1105 16-17 -0.19088297255780873 1105 18-19 0.18717095245927595 1105 20-21 0.8482309333655493 1105 22-23 0.5420763052704487 1105 24-25 0.1480280529805853 1105 26-27 0.10357104992613131 1105 28-29 0.6983231313549751 1105 30-31 0.7283468648467704 1105 32-33 0.9031882164509071 1105 34-35 1.061437325083915 1105 36-37 0.9273045235241426 1105 38-39 1.0663383927424235 1105 40-41 1.2555067411786283 1105 42-43 1.3548535057442734 1105 44-45 0.7004153286226042 1105 46-47 0.8961896420448952 1105 48-49 1.0189199533782798 1105 50-51 1.331000513475523 1105 52-53 1.7531928420279286 1105 54-55 1.6927388996001866 1105 56-57 1.7612146369263044 1105 58-59 1.3150410381737885 1105 60-61 2.0170850397929243 1105 62-63 2.325630453284006 1105 64-65 2.2391116544148844 1105 66-67 1.4645158968347083 1105 68-69 1.701544398246977 1105 70-71 2.2110942900040875 1105 72-73 1.5070110116604951 1105 74-75 1.434600336836482 1105 76-77 1.0671504303338217 1105 78-79 0.3914526567313956 1105 80-81 0.4108205611584168 1105 82-83 1.0344058444678055 1105 84-85 1.0140654351625606 1105 86-87 1.1804691054493937 1105 88-89 0.8447339825385711 1105 90-91 1.3428307436492375 1105 92-93 1.153761754456763 1105 94-95 0.8836876435967511 1105 96-97 0.8696678087496927 1105 98-99 1.2977484654747506 1105 100-101 1.6407097995258582 1106 1 0.04465102345259098 1106 2 0.21784334082119017 1106 3 0.2742425060586413 1106 4 0.41374716470672723 1106 5 0.330264790628064 1106 6 0.2992614707144128 1106 7 0.1812846008720399 1106 8 -0.22961391603131887 1106 9 -0.1267872208202192 1106 10-11 0.09847818465299696 1106 12-13 -0.174331490981956 1106 14-15 -0.28572578889831135 1106 16-17 0.028284470747571788 1106 18-19 0.07152944888674284 1106 20-21 0.28967237480699737 1106 22-23 0.44460814745746546 1106 24-25 0.2569127997963676 1106 26-27 0.055264122308862795 1106 28-29 0.8327592916532112 1106 30-31 0.5377597259837685 1106 32-33 0.6484507203341039 1106 34-35 0.5889303353355473 1106 36-37 0.6499668410140487 1106 38-39 -0.1920493053004506 1106 40-41 0.4930028579850898 1106 42-43 0.13273483534974417 1106 44-45 -0.19466078197074665 1106 46-47 -0.6270318553145344 1106 48-49 -0.5908967598867108 1106 50-51 -0.8354331615788411 1106 52-53 -1.3830295872979548 1106 54-55 -1.3220172358456068 1106 56-57 -1.8590090350215007 1106 58-59 -2.0682301640629284 1106 60-61 -2.187093276454359 1106 62-63 -1.985335685268339 1106 64-65 -1.9660762921523762 1106 66-67 -1.9161147337959186 1106 68-69 -2.5372720043625314 1106 70-71 -1.6627795838697885 1106 72-73 -1.5737594139375162 1106 74-75 -1.4864155065931506 1106 76-77 -1.1615696690759343 1106 78-79 -2.26247713512943 1106 80-81 -1.2598069629173843 1106 82-83 -1.2184677187505883 1106 84-85 -1.8878450960583137 1106 86-87 -2.102771031239019 1106 88-89 -1.6846229606114775 1106 90-91 -1.4094991724737795 1106 92-93 -0.9255330575966667 1106 94-95 -1.5629572771861007 1106 96-97 -0.7417021819306413 1106 98-99 -0.6662154984892155 1106 100-101 -0.457845652477868 1107 1 0.09182978115766716 1107 2 -0.44526713807876206 1107 3 -0.42419277132400524 1107 4 -0.8214353862652999 1107 5 -0.8008399983714583 1107 6 -0.31904104232494035 1107 7 -0.1129306670274417 1107 8 0.04753165058792774 1107 9 -0.1919839965433141 1107 10-11 -0.15318136963812634 1107 12-13 -0.20159559719343179 1107 14-15 -0.8922928823075154 1107 16-17 -1.463417757796762 1107 18-19 -1.1464648612128414 1107 20-21 -0.2394883648800601 1107 22-23 -0.7809726965444312 1107 24-25 -0.17623087018940709 1107 26-27 -1.0310327008300604 1107 28-29 -0.2835280483183382 1107 30-31 -1.0423730383595284 1107 32-33 -1.3714639311594965 1107 34-35 -1.6962759235549214 1107 36-37 -2.339483135278037 1107 38-39 -1.1281564650918199 1107 40-41 -1.0133982799949948 1107 42-43 -1.0649892044795592 1107 44-45 -1.2315265951523529 1107 46-47 -2.3049597832424595 1107 48-49 -2.5712191875775616 1107 50-51 -3.815399970493015 1107 52-53 -2.28582712167443 1107 54-55 -1.9270433145558883 1107 56-57 -2.775083003727051 1107 58-59 -2.628092658135948 1107 60-61 -3.6755949360086504 1107 62-63 -2.834079165590765 1107 64-65 -2.5267685633709647 1107 66-67 -3.4828525242179182 1107 68-69 -3.2555033936465527 1107 70-71 -2.5257478152590735 1107 72-73 -2.419183785677678 1107 74-75 -2.081128640779969 1107 76-77 -1.8684212575064656 1107 78-79 -1.79306034992317 1107 80-81 -1.442476474534267 1107 82-83 -1.4991694731365541 1107 84-85 -1.138082175242765 1107 86-87 -1.5061612635766188 1107 88-89 -1.8842436339163626 1107 90-91 -1.1229889780688502 1107 92-93 -1.1850162249745715 1107 94-95 -1.2999179220414838 1107 96-97 -1.4228306788486123 1107 98-99 -0.23733925382349597 1107 100-101 0.4189917112015955 1108 1 0.4746987182061666 1108 2 0.50003729948574 1108 3 0.21899608316515895 1108 4 0.025829835612924512 1108 5 0.2404396713911865 1108 6 0.2988640144346064 1108 7 0.16777108735852408 1108 8 0.017603890010015277 1108 9 0.32710785072190873 1108 10-11 0.5893366902173867 1108 12-13 0.4464952180800523 1108 14-15 0.3877638772384131 1108 16-17 0.16958017821974636 1108 18-19 0.7215691945147213 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-0.7403208340339944 2316 78-79 -0.9073979179812461 2316 80-81 -0.8592943813703187 2316 82-83 -0.8391573739230012 2316 84-85 -0.7445552544926102 2316 86-87 -0.9976918140908353 2316 88-89 -1.0690591209097065 2316 90-91 -1.7519968425576593 2316 92-93 -1.5215256018650756 2316 94-95 -1.96976063226532 2316 96-97 -1.0699873636641009 2316 98-99 -2.980987166709159 2316 100-101 -1.1696350280603482 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 3.0 6 4.0 7 12.0 8 27.0 9 62.0 10 115.0 11 195.0 12 304.0 13 418.0 14 558.0 15 787.0 16 1068.0 17 1447.0 18 1246.0 19 859.0 20 789.0 21 780.0 22 806.0 23 785.0 24 793.0 25 834.0 26 945.0 27 1052.0 28 1250.0 29 1461.0 30 1838.0 31 2158.0 32 2704.0 33 3633.0 34 5055.0 35 7419.0 36 10767.0 37 14844.0 38 11329.0 39 875.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.89684739528016 23.907468331476828 16.638603217366526 15.557081055876488 2 13.910363492741801 29.853800033668726 34.22943941571812 22.00639705787136 3 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32.66578609999611 30.760913199435404 14.76891599652953 48-49 20.619504551752716 31.170117711044636 30.84314258705308 17.36723515014957 50-51 14.967043497403623 32.88787019411315 34.4508760343421 17.694210274141124 52-53 19.16656954534271 34.64447120676483 28.306981080766093 17.881978167126373 54-55 17.291480517462414 31.322921927405044 29.768333268585785 21.617264286546753 56-57 20.339795138754 30.348471310360903 29.723657459565157 19.588076091319945 58-59 23.724797016432927 29.37143079134455 28.555611670098287 18.34816052212424 60-61 18.561827434831592 33.65966098183184 31.79104670888207 15.987464874454501 62-63 17.121841938282635 31.609753570827344 32.352407961358665 18.915996529531355 64-65 16.8382476723256 35.9899252813281 29.20697201610919 17.964855030237107 66-67 19.112829079419345 30.593217046734782 27.89130181422633 22.402652059619545 68-69 17.08504645666742 31.19557123895238 29.567159830360325 22.15222247401988 70-71 21.60560972767181 31.65701928182018 27.81878455900444 18.918586431503567 72-73 20.06720795617886 27.60511764629709 32.90599950791863 19.421674889605427 74-75 16.899110368672545 35.95690403118242 27.94115742719138 19.202828172953655 76-77 18.950312730663143 29.381142923740338 29.185605324838455 22.482939020758064 78-79 18.617510327234115 31.037385234968855 29.876461675925565 20.468642761871465 80-81 21.015112078007846 30.06811442186913 28.558201572070494 20.358571928052523 82-83 19.205267997047436 28.16138097149739 32.067701790963596 20.565649240491577 84-85 17.759187796223873 31.31685271036751 30.128072310999453 20.79588718240916 86-87 17.96097017727879 31.499035261515353 28.561438949535763 21.978555611670096 88-89 17.55370809214871 31.442057418126723 26.69994690700957 24.304287582714995 90-91 19.950915954904843 27.2066775022826 28.500475946875266 24.341930595937292 92-93 21.239688685720207 31.31984822781368 28.106344129187654 19.33411895727846 94-95 20.293176903254214 29.10661331468604 28.512230812063766 22.087978969995987 96-97 15.884516271059141 30.330341996555433 31.361770456988204 22.423371275397226 98-99 19.350828460985607 31.86936280699028 26.22585678858867 22.553951943435443 100-101 17.555709539752748 29.38989878057468 27.559141804335013 25.49524987533756 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 602.0 1 361.0 2 130.0 3 139.0 4 117.0 5 125.5 6 182.5 7 254.5 8 315.0 9 337.5 10 325.5 11 283.5 12 277.0 13 279.0 14 248.5 15 236.5 16 238.0 17 237.5 18 240.5 19 253.5 20 269.0 21 294.0 22 318.5 23 336.5 24 365.0 25 432.5 26 560.0 27 703.0 28 727.0 29 853.0 30 1170.5 31 1412.5 32 1855.5 33 2390.0 34 2648.0 35 3018.0 36 3631.0 37 3835.0 38 3500.0 39 3120.5 40 2814.5 41 2385.5 42 1867.5 43 1782.0 44 1814.5 45 1668.0 46 1759.5 47 3983.0 48 8589.0 49 7881.0 50 3142.5 51 1155.5 52 594.5 53 363.5 54 203.0 55 164.5 56 144.0 57 110.5 58 91.5 59 78.0 60 72.0 61 65.5 62 45.5 63 28.0 64 23.0 65 18.5 66 13.5 67 13.0 68 6.5 69 2.5 70 3.0 71 2.5 72 2.5 73 3.5 74 2.0 75 1.0 76 1.5 77 2.0 78 1.0 79 1.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02201416676378799 2 0.0 3 0.0 4 0.0 5 0.023309117749893166 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0012949509861051758 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.474754930525879E-4 24-25 0.0 26-27 6.474754930525879E-4 28-29 0.001942426479157764 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.014891936340209523 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 6.474754930525879E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0025899019722103516 84-85 0.0012949509861051758 86-87 0.0 88-89 0.0 90-91 0.011007083381893995 92-93 0.0025899019722103516 94-95 0.0 96-97 0.0 98-99 0.001942426479157764 100-101 0.01877678929852505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 77223.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.082307084676845 #Duplication Level Percentage of deduplicated Percentage of total 1 71.68219127547727 20.13001307900496 2 10.495250391957946 5.894616888750761 3 4.860278520704602 4.094635018064566 4 2.683759107258139 3.0146458956528495 5 1.8398967075532604 2.583427217279826 6 1.3695471732915245 2.3076026572394235 7 0.9084201789172738 1.7857374098390375 8 0.7700820806049986 1.7300545174365152 9 0.6870792216176336 1.736529272367041 >10 4.279258507793046 22.042655685482305 >50 0.23978603707461035 4.612615412506637 >100 0.11067047864982016 7.32035792445256 >500 0.04611269943742507 9.490695777164834 >1k 0.02766761966245504 13.256413244758686 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4200 5.438794141641739 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1333 1.7261696644781994 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1325 1.7158100565893581 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1228 1.590199810937156 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1089 1.4102016238685364 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1062 1.3752379472436969 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 986 1.2768216722997034 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 935 1.2107791720083396 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 916 1.186175103272341 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 823 1.0657446615645598 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 651 0.8430130919544695 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 651 0.8430130919544695 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 649 0.8404231899822592 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 610 0.7899201015241574 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 595 0.7704958367325797 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 513 0.6643098558719552 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 480 0.6215764733304844 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 423 0.5477642671224894 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 411 0.5322248552892272 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 384 0.49726117866438757 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 361 0.4674773059839685 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 350 0.4532328451368116 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 272 0.35222666822060783 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 263 0.3405721093456613 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 259 0.33539230540124054 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 212 0.2745296090542973 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 210 0.2719397070820869 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 205 0.2654649521515611 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 193 0.24992554031829897 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 189 0.24474573637387828 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 189 0.24474573637387828 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 175 0.2266164225684058 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 166 0.2149618636934592 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 164 0.21237196172124886 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 158 0.2046022558046178 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 127 0.16445877523535735 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 125 0.161868873263147 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 121 0.15668906931872628 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 111 0.14373955945767453 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 105 0.13596985354104346 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 100 0.12949509861051758 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 93 0.12043044170778137 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 91 0.11784053973557101 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 89 0.11525063776336067 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 88 0.11395568677725548 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 87 0.11266073579115031 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 84 0.10877588283283478 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 83 0.1074809318467296 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 82 0.10618598086062443 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 82 0.10618598086062443 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 80 0.10359607888841407 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 79 0.1023011279023089 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 79 0.1023011279023089 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0019424264791577637 0.0 24-25 0.0 0.0 0.0 0.008417181409683644 0.0 26-27 0.0 0.0 0.0 0.011654558874946583 0.0 28-29 0.0 0.0 0.0 0.03496367662483975 0.0 30-31 0.0 0.0 0.0 0.06733745127746915 0.0 32-33 0.0 0.0 0.0 0.09453142198567785 0.0 34-35 0.0 0.0 0.0 0.12107791720083394 0.0 36-37 0.0 0.0 0.0 0.14179713297851676 0.0 38-39 0.0 0.0 0.0 0.17028605467283064 0.0 40-41 0.0 0.0 0.0 0.20201235383240745 0.0 42-43 0.0 0.0 0.0 0.27711951102650767 0.0 44-45 0.0 0.0 0.0 0.3813630654079743 0.0 46-47 0.0 0.0 0.0 0.47006720795617885 0.0 48-49 0.0 0.0 0.0 0.6461805420664828 0.0 50-51 0.0 0.0 0.0 0.8222938761767866 0.0 52-53 0.0 0.0 0.0 0.9595586807039354 0.0 54-55 0.0 0.0 0.0 1.184880152286236 0.0 56-57 0.0 0.0 0.0 1.4639420897919013 0.0 58-59 0.0 0.0 0.0 1.700270644756096 0.0 60-61 0.0 0.0 0.0 1.9560234645118681 0.0 62-63 0.0 0.0 0.0 2.3024228532950026 0.0 64-65 0.0 0.0 0.0 2.578247413335405 0.0 66-67 0.0 0.0 0.0 2.9498983463475907 0.0 68-69 0.0 0.0 0.0 3.4355049661370316 0.0 70-71 0.0 0.0 0.0 3.804565997177007 0.0 72-73 0.0 0.0 0.0 4.24549680794582 0.0 74-75 0.0 0.0 0.0 4.920166271706616 0.0 76-77 0.0 0.0 0.0 5.340377866697745 0.0 78-79 0.0 0.0 0.0 5.852530981702342 0.0 80-81 0.0 0.0 0.0 6.514250935602087 0.0 82-83 0.0 0.0 0.0 7.111870815689626 0.0 84-85 0.0 0.0 0.0 7.8467555003043135 0.0 86-87 0.0 0.0 0.0 8.63149579788405 0.0 88-89 0.0 0.0 0.0 9.804073915802288 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATCAAT 45 9.540397E-4 42.248146 1 AGGAGTG 50 0.0016016503 38.023335 5 GTGTGGT 50 0.0016067628 37.998707 9 CTATAGA 55 0.0025570905 34.566666 1 GAACTAG 55 0.0025652384 34.544277 6 ATCAATA 60 0.003916559 31.686111 2 CAATACT 80 4.439333E-4 29.705725 4 TACTTAT 80 4.4562362E-4 29.686487 7 AGGCTGC 65 0.005811774 29.229774 8 ACTAGTA 65 0.005811774 29.229774 8 AGGAGGC 70 0.008319037 27.159523 5 TTATTGC 35 0.0083708735 27.141933 14-15 TGCCTAT 35 0.0083708735 27.141933 18-19 AACTAGG 35 0.0083708735 27.141933 64-65 CTAGTAC 70 0.008345404 27.141933 9 ATCTAGG 110 9.325779E-5 25.925 1 TCTAGGA 180 1.8911851E-7 23.764582 2 CTACAAA 50 0.001647519 23.749191 24-25 ACGTCTA 50 0.001647519 23.749191 20-21 AATGTCA 50 0.001647519 23.749191 30-31 >>END_MODULE