FastQCFastQC Report
Thu 26 May 2016
SRR937847_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937847_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390468
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50420.3626117249731745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39680.2853715439693686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26200.18842576743945202No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19140.13765149575538593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31900.044.575081
GTATCAA54000.039.1902351
ATCAACG69100.030.4076963
TATCAAC70800.030.0132872
TCAACGC70500.029.8766694
CAACGCA71600.029.3512675
AACGCAG73000.028.6983836
GTACATG60300.025.000781
ACGCAGA83650.024.9896287
CGCAGAG84150.024.6144288
TACATGG63100.023.6559052
GCAGAGT90600.022.390969
ACATGGG63650.022.2565143
GAGTACT46350.022.13478512-13
GTGGTAT19050.020.7202361
TGGTATC17900.020.449232
GTGTAAG4700.020.2368891
AGTACTT48850.020.22413812-13
AGAGTAC84200.019.82861310-11
TAGGGCC2902.410161E-919.674374
Produced by FastQC (version 0.11.5)