##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937847_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1390468 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75986502386247 33.0 31.0 34.0 30.0 34.0 2 31.894951915470187 33.0 31.0 34.0 30.0 34.0 3 31.9254387731325 34.0 31.0 34.0 30.0 34.0 4 35.503949030110725 37.0 35.0 37.0 33.0 37.0 5 35.49386609400576 37.0 35.0 37.0 33.0 37.0 6 35.55937784975994 37.0 35.0 37.0 33.0 37.0 7 35.49853358725264 37.0 35.0 37.0 33.0 37.0 8 35.48149471976342 37.0 35.0 37.0 33.0 37.0 9 37.124751522508966 39.0 37.0 39.0 34.0 39.0 10-11 36.99862600218056 39.0 37.0 39.0 33.0 39.0 12-13 36.8821310522788 39.0 37.0 39.0 33.0 39.0 14-15 38.06248220023762 40.0 38.0 41.0 33.0 41.0 16-17 37.978804618301176 40.0 38.0 41.0 33.0 41.0 18-19 37.9042326036989 40.0 38.0 41.0 33.0 41.0 20-21 37.80203931338225 40.0 38.0 41.0 33.0 41.0 22-23 37.71821969293792 40.0 37.5 41.0 32.5 41.0 24-25 37.75955469669205 40.0 38.0 41.0 33.0 41.0 26-27 37.68489817816735 40.0 37.5 41.0 32.0 41.0 28-29 37.56003913790177 40.0 37.0 41.0 32.0 41.0 30-31 37.32838691721061 40.0 37.0 41.0 32.0 41.0 32-33 37.20553331684008 39.5 37.0 41.0 31.5 41.0 34-35 37.20806627696574 40.0 37.0 41.0 31.0 41.0 36-37 37.08588079696908 40.0 36.5 41.0 31.0 41.0 38-39 36.94653778440065 40.0 36.0 41.0 31.0 41.0 40-41 36.715244076095246 39.5 36.0 41.0 30.0 41.0 42-43 36.455992514750434 39.0 36.0 41.0 30.0 41.0 44-45 36.229751781414606 39.0 35.5 41.0 30.0 41.0 46-47 35.88858930949867 39.0 35.0 41.0 28.5 41.0 48-49 35.58831990380217 39.0 35.0 40.5 27.0 41.0 50-51 34.7187446960304 38.0 34.0 39.5 26.0 40.5 52-53 34.81268429046911 38.0 34.5 40.0 26.0 40.5 54-55 35.09530028738526 39.0 35.0 40.0 26.0 41.0 56-57 34.879739411478724 38.0 34.0 40.0 26.0 41.0 58-59 34.63707866703872 38.0 34.0 40.0 25.0 41.0 60-61 34.312021204371476 38.0 34.0 40.0 23.5 41.0 62-63 33.94039920372134 37.5 33.5 40.0 23.0 41.0 64-65 33.54529086609688 37.0 33.0 40.0 22.0 41.0 66-67 33.11010069990823 36.0 32.5 39.0 20.5 41.0 68-69 32.608585382763216 36.0 32.0 39.0 19.5 40.0 70-71 32.155145965243356 35.0 31.5 38.5 18.0 40.0 72-73 31.691613183474914 35.0 31.0 38.0 15.0 40.0 74-75 31.153689980639612 35.0 31.0 37.0 10.0 39.0 76-77 30.666291133632704 34.5 30.0 36.5 7.5 39.0 78-79 30.113200016109687 34.0 29.5 36.0 4.5 38.5 80-81 29.556373825215683 34.0 29.0 35.5 2.0 37.0 82-83 29.050423670303815 34.0 29.0 35.0 2.0 37.0 84-85 28.539314101439228 34.0 28.5 35.0 2.0 36.5 86-87 27.99580680749215 33.5 27.0 35.0 2.0 36.0 88-89 27.527022916025395 33.0 26.0 35.0 2.0 35.5 90-91 26.98402408397748 33.0 25.5 35.0 2.0 35.0 92-93 26.371550082418295 32.0 25.0 35.0 2.0 35.0 94-95 25.62845315390214 32.0 22.0 34.0 2.0 35.0 96-97 24.804571554325594 31.5 18.5 34.0 2.0 35.0 98-99 23.759770091796433 31.0 2.0 34.0 2.0 35.0 100-101 20.928233515622075 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.266549791970526 1101 2 0.3604762060697375 1101 3 0.31517386707094985 1101 4 0.11559699848712057 1101 5 0.14941542975491018 1101 6 0.29525168808455504 1101 7 0.3324990838011672 1101 8 0.4210208923573333 1101 9 0.3568422194896712 1101 10-11 0.48961706694831264 1101 12-13 0.4431797569471314 1101 14-15 0.5068382266616709 1101 16-17 0.6501771855629883 1101 18-19 0.6509121789300849 1101 20-21 0.6568208803900291 1101 22-23 0.611269918210624 1101 24-25 0.6018098144677495 1101 26-27 0.4429996327828363 1101 28-29 0.49027704235220426 1101 30-31 0.5360193428703042 1101 32-33 0.4076418511549349 1101 34-35 0.5580880559943395 1101 36-37 0.4679064523548888 1101 38-39 0.45744899066293243 1101 40-41 0.5254193751094007 1101 42-43 0.746351050686215 1101 44-45 0.7797726006102152 1101 46-47 0.6954776238800235 1101 48-49 0.8760126762231053 1101 50-51 0.9570402152873214 1101 52-53 0.8111003856555783 1101 54-55 1.0196321292712724 1101 56-57 0.7480146620566472 1101 58-59 0.8023392489847723 1101 60-61 0.8931165801590808 1101 62-63 0.9536157337229199 1101 64-65 0.9616833419506747 1101 66-67 1.032593244824298 1101 68-69 1.0456149454645711 1101 70-71 1.073123391454935 1101 72-73 1.092625743581877 1101 74-75 1.09397241035893 1101 76-77 0.895485707429355 1101 78-79 1.0241926241988466 1101 80-81 1.243641462832482 1101 82-83 1.1818280804011643 1101 84-85 1.1633596900099938 1101 86-87 1.295744297627781 1101 88-89 1.164573390993528 1101 90-91 1.286985711018371 1101 92-93 1.2039041544684501 1101 94-95 1.4158701949856898 1101 96-97 1.7231057761512183 1101 98-99 1.7820545043961964 1101 100-101 1.6628709405067816 1102 1 0.22931798449615215 1102 2 0.3248827339447864 1102 3 0.3524948067793652 1102 4 0.2089513415613311 1102 5 0.11276828729188537 1102 6 0.13204313987887417 1102 7 0.034399510362575825 1102 8 0.20289946029944161 1102 9 0.1990558821878281 1102 10-11 0.30613783263664374 1102 12-13 0.31199673875990896 1102 14-15 0.2935516877512043 1102 16-17 0.37217427815439663 1102 18-19 0.26236460990566 1102 20-21 0.5254580060877032 1102 22-23 0.5337460965599803 1102 24-25 0.37809745692016605 1102 26-27 0.3425682964359851 1102 28-29 0.3609116850804952 1102 30-31 0.47161334554427015 1102 32-33 0.5982007314842974 1102 34-35 0.6927986945631233 1102 36-37 0.5932162929779707 1102 38-39 0.5412773262245665 1102 40-41 0.6054388993130431 1102 42-43 0.5926419825424958 1102 44-45 0.858388292127799 1102 46-47 0.8317051020744657 1102 48-49 0.7008821611661276 1102 50-51 0.5684952990079566 1102 52-53 0.6240866338251863 1102 54-55 0.542582087888448 1102 56-57 0.5133825447416527 1102 58-59 0.5707259555487312 1102 60-61 0.734630979259272 1102 62-63 0.8117432140395593 1102 64-65 0.7420074012872817 1102 66-67 0.687646724164722 1102 68-69 0.7480963231942397 1102 70-71 0.6800592586903491 1102 72-73 0.675088976535335 1102 74-75 0.6471127299616626 1102 76-77 0.8529913843037846 1102 78-79 0.9365655577104448 1102 80-81 1.0661659211419412 1102 82-83 1.1207693167925825 1102 84-85 1.2015406496670487 1102 86-87 1.0260842734347477 1102 88-89 1.1270354567391614 1102 90-91 1.3144522333757074 1102 92-93 1.2434884162625544 1102 94-95 1.462074647352999 1102 96-97 1.4713443534309896 1102 98-99 1.8906043017264729 1102 100-101 1.935491428187433 1103 1 0.4003960474189512 1103 2 0.32166143039061623 1103 3 0.351608948301962 1103 4 0.13574184945352386 1103 5 0.2459262385428289 1103 6 0.2979241664009393 1103 7 0.29320440674397474 1103 8 0.303264541368911 1103 9 0.3618283507801223 1103 10-11 0.3480362541979005 1103 12-13 0.4596747157812828 1103 14-15 0.5623802070253419 1103 16-17 0.5608836091970275 1103 18-19 0.4717922916408668 1103 20-21 0.5434650929555218 1103 22-23 0.5189549444070849 1103 24-25 0.5298906492319873 1103 26-27 0.48511097870807873 1103 28-29 0.46218409998439824 1103 30-31 0.5945221826536908 1103 32-33 0.5712276830573728 1103 34-35 0.5449193860696226 1103 36-37 0.4899896205845451 1103 38-39 0.5123580735669933 1103 40-41 0.6856117759371188 1103 42-43 0.6000053455834049 1103 44-45 0.5572876800801296 1103 46-47 0.6760812429528045 1103 48-49 0.7599877125459145 1103 50-51 0.695017364937307 1103 52-53 0.6464424804911388 1103 54-55 0.6822658632562195 1103 56-57 0.6985242820980346 1103 58-59 0.7536798909078968 1103 60-61 0.8421446697993744 1103 62-63 1.0987264632610731 1103 64-65 1.248197672950539 1103 66-67 1.2002098080259884 1103 68-69 1.0595265819722997 1103 70-71 0.9671257249203506 1103 72-73 0.8248473787687729 1103 74-75 0.7735193658469832 1103 76-77 0.9926778440911797 1103 78-79 1.0553564951097805 1103 80-81 1.4089850986357675 1103 82-83 1.4687452643927124 1103 84-85 1.5316839976752092 1103 86-87 1.409467715934479 1103 88-89 1.3328096433600116 1103 90-91 1.2835867764874855 1103 92-93 1.2617102567006526 1103 94-95 1.1786536229784694 1103 96-97 1.1818672783746145 1103 98-99 1.4436403803262756 1103 100-101 1.526455671717983 1104 1 0.2169868737749745 1104 2 0.34520757163560845 1104 3 0.21266874080287224 1104 4 0.144166949562738 1104 5 0.23508031571792998 1104 6 0.12097431201433295 1104 7 0.2133673117312398 1104 8 0.24500553154000926 1104 9 0.18315616293883608 1104 10-11 0.24563818357540868 1104 12-13 0.3136324423921266 1104 14-15 0.36053273705810085 1104 16-17 0.2710446940168296 1104 18-19 0.2383307881825445 1104 20-21 0.3028071024203953 1104 22-23 0.271764827916364 1104 24-25 0.35712831759747843 1104 26-27 0.32318660666584975 1104 28-29 0.23902133150969007 1104 30-31 0.3395315110118986 1104 32-33 0.2843101366431 1104 34-35 0.21088794267682687 1104 36-37 0.25563623507307653 1104 38-39 0.3946364681792929 1104 40-41 0.3730253179829859 1104 42-43 0.3259745847534177 1104 44-45 0.3397139632981805 1104 46-47 0.6091775753153783 1104 48-49 0.7055271646755088 1104 50-51 0.5801886881990441 1104 52-53 0.4457121382996547 1104 54-55 0.6696361763250138 1104 56-57 0.7144803250539837 1104 58-59 0.595929390361853 1104 60-61 0.6136146825405504 1104 62-63 0.7990677446589629 1104 64-65 0.8868398280288119 1104 66-67 0.9748890035119757 1104 68-69 0.9418720952595194 1104 70-71 0.7229127198238814 1104 72-73 0.8845363576548237 1104 74-75 0.9692624492106461 1104 76-77 0.9149430424894298 1104 78-79 0.868486728091213 1104 80-81 1.1255326960147265 1104 82-83 0.9650139211091293 1104 84-85 0.9259550206093188 1104 86-87 1.1212555754175533 1104 88-89 1.041631767495435 1104 90-91 1.0788873316385583 1104 92-93 1.087109555936184 1104 94-95 1.2629410274196786 1104 96-97 1.2400921600637282 1104 98-99 1.513719102568725 1104 100-101 1.370042994251655 1105 1 0.3967637563854325 1105 2 0.47320461942282677 1105 3 0.4254062513793393 1105 4 0.3273156546357612 1105 5 0.30504017114227366 1105 6 0.43662770751888047 1105 7 0.4667540791961926 1105 8 0.42074726350484326 1105 9 0.5728321552869957 1105 10-11 0.5952661679774209 1105 12-13 0.6438347977245087 1105 14-15 0.5933666773884525 1105 16-17 0.5717174490475472 1105 18-19 0.5961129958123124 1105 20-21 0.5752361021628758 1105 22-23 0.7228337404720051 1105 24-25 0.6625587566586546 1105 26-27 0.6433143059632087 1105 28-29 0.5197672250828873 1105 30-31 0.6699189544508144 1105 32-33 0.5077149300801764 1105 34-35 0.6241994563066697 1105 36-37 0.7269912639514118 1105 38-39 0.75709761117308 1105 40-41 0.721768098397213 1105 42-43 0.7919252708439828 1105 44-45 0.5239329604655296 1105 46-47 0.538156049913475 1105 48-49 0.7841384520610077 1105 50-51 0.641037265434818 1105 52-53 0.5343164590148035 1105 54-55 0.631334434427508 1105 56-57 0.5761390363809156 1105 58-59 0.5314066417689816 1105 60-61 0.622374178365142 1105 62-63 0.899762572308596 1105 64-65 0.6414474872275733 1105 66-67 0.8741838293222202 1105 68-69 0.8736809233873899 1105 70-71 0.9524547359736815 1105 72-73 0.8764355915510151 1105 74-75 0.9108930179484673 1105 76-77 0.9364983343998183 1105 78-79 0.870524996494261 1105 80-81 1.0415682237342558 1105 82-83 0.8877137702795679 1105 84-85 0.969762271464635 1105 86-87 1.0741983613499357 1105 88-89 1.2468272251343997 1105 90-91 1.2952513045260226 1105 92-93 1.3071189597915343 1105 94-95 1.4488591563973507 1105 96-97 1.6529800403565567 1105 98-99 1.787698933713191 1105 100-101 1.9526572430468576 1106 1 0.204050311310759 1106 2 0.2079272842200588 1106 3 0.06590717547595304 1106 4 0.23556666901767898 1106 5 0.24310232991694392 1106 6 0.1900936009184946 1106 7 0.24021232489968014 1106 8 0.3302983862291171 1106 9 0.2758147567782956 1106 10-11 0.1821333742315403 1106 12-13 0.1603771893290329 1106 14-15 0.35458117402134093 1106 16-17 0.29626655362979193 1106 18-19 0.30848208212506023 1106 20-21 0.20817324234939605 1106 22-23 0.34024287097143713 1106 24-25 0.43548591235300194 1106 26-27 0.3388563364265238 1106 28-29 0.44854805485790905 1106 30-31 0.4399569804954666 1106 32-33 0.3663366452884702 1106 34-35 0.45263082885569617 1106 36-37 0.404855432370681 1106 38-39 0.43457620824570853 1106 40-41 0.4080734114216611 1106 42-43 0.7441770924827154 1106 44-45 0.5334246011087913 1106 46-47 0.7070724138588389 1106 48-49 0.6359144170515592 1106 50-51 0.6701452466554727 1106 52-53 0.5869687225204245 1106 54-55 0.6150193493468876 1106 56-57 0.6641661432149846 1106 58-59 0.6338985160918398 1106 60-61 0.7143908333478279 1106 62-63 0.8144419001610785 1106 64-65 0.8961550690685414 1106 66-67 0.9089372973399747 1106 68-69 0.8760846367687378 1106 70-71 0.6266310153695116 1106 72-73 0.7353196652238196 1106 74-75 0.8182411793148532 1106 76-77 0.8263895671659469 1106 78-79 1.0618416748449064 1106 80-81 0.7957011661853421 1106 82-83 0.7410702591372988 1106 84-85 0.8661402376023339 1106 86-87 1.2650699862729269 1106 88-89 1.1043060346430345 1106 90-91 1.24958075028049 1106 92-93 1.1103543601271824 1106 94-95 1.5922651428439316 1106 96-97 1.7928510917023424 1106 98-99 1.7759979586319297 1106 100-101 1.7012647250429076 1107 1 0.19430463704961554 1107 2 0.25430178560725736 1107 3 0.21285667088548976 1107 4 0.16113549561238472 1107 5 0.095889437905754 1107 6 0.2839417341804733 1107 7 0.27854146438222216 1107 8 0.20085983088736725 1107 9 0.39817652100418144 1107 10-11 0.2862027378668657 1107 12-13 0.28631264577560955 1107 14-15 0.23512360402578736 1107 16-17 0.37187010758624695 1107 18-19 0.3783041945354455 1107 20-21 0.43073388239555044 1107 22-23 0.35977710683664554 1107 24-25 0.3919154082179759 1107 26-27 0.31070250370235186 1107 28-29 0.2872555897465787 1107 30-31 0.32004131295207117 1107 32-33 0.299057410732658 1107 34-35 0.2617119390685687 1107 36-37 0.22588351409194019 1107 38-39 0.24356471216059816 1107 40-41 0.39219788339497796 1107 42-43 0.40806860648182663 1107 44-45 0.1791538946821447 1107 46-47 -5.584969089227343E-4 1107 48-49 0.17775880051753745 1107 50-51 0.19291007599800736 1107 52-53 0.20869286961262645 1107 54-55 0.22161147553435967 1107 56-57 0.35248939992040107 1107 58-59 0.29308191071991985 1107 60-61 0.2943150024530041 1107 62-63 0.3011984404808814 1107 64-65 0.43902647156056673 1107 66-67 0.3476011858177088 1107 68-69 0.4962439651453536 1107 70-71 0.508254358229145 1107 72-73 0.525177377924166 1107 74-75 0.4534892617192767 1107 76-77 0.43993589498465013 1107 78-79 0.7798964871463632 1107 80-81 0.6299943410252737 1107 82-83 0.17358303994502222 1107 84-85 0.2842163038082468 1107 86-87 0.3172851365114653 1107 88-89 0.2729986538451783 1107 90-91 0.8452923955224598 1107 92-93 0.7664931329419211 1107 94-95 1.1187524417383479 1107 96-97 1.4147543788463857 1107 98-99 1.398388567442435 1107 100-101 1.4663885859664667 1108 1 0.2056662082427394 1108 2 0.12585621872285557 1108 3 0.18131763998566797 1108 4 0.22653851213495102 1108 5 0.05969074932226448 1108 6 0.30948072469474397 1108 7 0.22366515211274418 1108 8 0.2669654168365341 1108 9 0.350483267411434 1108 10-11 0.37276917872257087 1108 12-13 0.4506696763788085 1108 14-15 0.4467940550976408 1108 16-17 0.5106470658157392 1108 18-19 0.5996079934933576 1108 20-21 0.41915576106201513 1108 22-23 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-1.3388804175718825 2316 98-99 -1.5779483970980763 2316 100-101 -1.4195224295788123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5574.0 3 1093.0 4 1015.0 5 1168.0 6 1302.0 7 1536.0 8 1927.0 9 2380.0 10 2941.0 11 3695.0 12 4984.0 13 6304.0 14 7097.0 15 8793.0 16 11019.0 17 14207.0 18 12300.0 19 8969.0 20 9096.0 21 9667.0 22 10409.0 23 11799.0 24 13173.0 25 14998.0 26 17441.0 27 20589.0 28 24271.0 29 29168.0 30 35473.0 31 44632.0 32 56447.0 33 72957.0 34 98766.0 35 138002.0 36 200987.0 37 270892.0 38 196884.0 39 18512.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.673062090034264 18.77189400110683 14.735064097337617 22.81997981152129 2 14.993688735655397 19.817297913315684 41.451879314199985 23.737134036828934 3 19.0490753516794 24.401786184407456 29.99865120140448 26.550487262508664 4 12.607165886950538 16.258848443669297 37.54957075941899 33.58441490996118 5 13.743936000230839 37.18500564470462 34.446620571251316 14.624437783813224 6 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29.60319867879405 21.748234465057863 28-29 21.67436101810467 26.675773530950607 29.949308084906434 21.70055736603829 30-31 21.788775443282283 26.479211154649175 29.904608636680415 21.827404765388128 32-33 21.66320121368932 26.680984933387624 29.687640765258504 21.96817308766455 34-35 22.159972958987318 26.60123587229608 29.4591004683169 21.779690700399705 36-37 22.171137813342327 26.410747467935348 29.438237220431745 21.97987749829058 38-39 22.003651989664345 26.613811639032992 29.617355200122763 21.7651811711799 40-41 22.08548224169509 26.524842317319376 29.56328706245488 21.82638837853066 42-43 22.01124757749058 26.48585081557367 29.365768207957327 22.137133398978428 44-45 22.01028758542962 26.78706802374014 29.113088495141575 22.08955589568867 46-47 22.05439579603991 26.95925612044588 28.723145129442422 22.263202954071794 48-49 22.32563334810635 26.9934025863591 28.53120169493358 22.14976237060097 50-51 22.18236562270402 27.18354210304238 28.350942103114445 22.283150171139155 52-53 22.451277415801854 27.219808648929966 28.23077028428746 22.098143650980724 54-55 22.265356194538818 27.07276651090399 28.301334130761386 22.360543163795807 56-57 22.170877897889397 27.517543944965993 28.240591146562227 22.07098701058238 58-59 22.317057312070006 27.073460932037534 28.33417445742093 22.27530729847153 60-61 22.360708433695013 26.81901830878007 28.731014127538668 22.089259129986253 62-63 22.32744776367806 26.995268633134266 28.593320753819786 22.083962849367893 64-65 22.285433311960116 26.989402246349144 28.46606341361498 22.25910102807576 66-67 22.08327194698525 27.35207571107004 28.295762486883735 22.268889855060976 68-69 22.040932341780838 27.17673043454453 28.48267322898328 22.299663994691354 70-71 22.01537687316259 27.221417103178077 28.34310823501695 22.420097788642384 72-73 22.13936378795045 27.10840834599204 28.295222736175056 22.457005129882457 74-75 22.0839253139058 27.281094470012885 28.223100066570794 22.41188014951052 76-77 22.12045430638591 27.21717165167346 28.227249737384742 22.435124304555888 78-79 22.15244869418108 27.159246719691506 28.159244558157166 22.529060027970253 80-81 21.960638952408534 27.41890978673249 28.25729707454205 22.36315418631693 82-83 22.063783502224567 27.35076171260789 28.157448342913504 22.428006442254038 84-85 22.055565841257124 27.180054447781494 28.095934138211494 22.66844557274989 86-87 22.23938721805324 26.95469041415225 28.242149145524188 22.563773222270328 88-89 22.26873336054121 26.86551949331396 28.22714997746885 22.63859716867598 90-91 21.978107879895216 27.051890938538374 28.393977469545845 22.576023712020564 92-93 22.142877468607395 26.90667243952279 28.332608724625036 22.617841367244782 94-95 22.01894678524587 26.834891219344037 28.293524960096768 22.85263703531332 96-97 22.051799286769207 26.82147617160765 28.220417131947695 22.906307409675446 98-99 21.957797559696374 26.87089704500787 28.13413168648381 23.03717370881195 100-101 22.196391353625952 26.187338584582847 28.810394261489463 22.805875800301735 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1792.0 1 1023.0 2 258.0 3 259.0 4 211.5 5 211.5 6 328.0 7 685.5 8 1180.5 9 1410.5 10 1307.5 11 1201.0 12 1268.5 13 1421.0 14 1567.5 15 1647.0 16 1743.0 17 1853.0 18 1956.5 19 2053.5 20 2258.5 21 2666.0 22 3237.0 23 3961.0 24 4867.0 25 6152.5 26 7794.0 27 9501.0 28 11057.0 29 13019.5 30 15632.5 31 18424.0 32 21355.5 33 24214.5 34 27640.0 35 30225.5 36 32591.0 37 36013.0 38 38351.5 39 39894.5 40 42292.0 41 46442.5 42 49921.5 43 52695.5 44 55317.0 45 56252.0 46 55492.5 47 55073.0 48 57912.5 49 58016.0 50 54922.5 51 52182.0 52 48933.5 53 46811.5 54 44382.5 55 40337.0 56 35953.5 57 31545.0 58 27519.0 59 23869.5 60 19753.5 61 15707.0 62 12119.5 63 9091.0 64 6687.0 65 4951.5 66 3777.5 67 2997.5 68 2213.5 69 1496.0 70 1078.0 71 838.5 72 679.0 73 517.0 74 377.0 75 299.5 76 214.0 77 138.0 78 90.0 79 63.0 80 43.5 81 34.0 82 23.5 83 16.5 84 14.0 85 12.5 86 7.0 87 4.0 88 3.5 89 4.5 90 3.5 91 1.5 92 1.5 93 1.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3257176720356024 2 0.2918441848356093 3 0.29119692074898523 4 0.2924195306903863 5 0.3029915107719128 6 0.20417586021397113 7 0.20309708673626437 8 0.20396010551842975 9 0.20381626905473554 10-11 0.21000123699358775 12-13 0.1993932977961377 14-15 0.20047207127384448 16-17 0.20701663037193233 18-19 0.1995011751439084 20-21 0.1920935972636551 22-23 0.1912305784814897 24-25 0.19507820388531055 26-27 0.19022372323563003 28-29 0.20640532540123183 30-31 0.21007315522543488 32-33 0.2130937209630139 34-35 0.21187111102161288 36-37 0.18475793761524895 38-39 0.1767390547642952 40-41 0.18367916413754218 42-43 0.19435902156683937 44-45 0.19964501160760262 46-47 0.20309708673626437 48-49 0.20158680386747485 50-51 0.20529059280760148 52-53 0.22834038611460314 54-55 0.19223743372734936 56-57 0.1764513818369067 58-59 0.17580411775028262 60-61 0.169978740970666 62-63 0.17957982492225638 64-65 0.17533664924327638 66-67 0.17271163378085652 68-69 0.18292402270314742 70-71 0.17796166470569622 72-73 0.17436575311334024 74-75 0.1777818691260784 76-77 0.18022708900888046 78-79 0.18447026468786049 80-81 0.17591199509805333 82-83 0.17580411775028262 84-85 0.16893592660888276 86-87 0.16591536087130376 88-89 0.17184861499869108 90-91 0.17835721498085536 92-93 0.16990682273881888 94-95 0.174221916649646 96-97 0.173898284606334 98-99 0.17576815863435907 100-101 0.15559509460124218 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1390468.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.69453628869227 #Duplication Level Percentage of deduplicated Percentage of total 1 77.47409998271539 45.47306372869261 2 13.00379574077046 15.265035219947876 3 4.248002591356315 7.480036267584661 4 1.8391774579416689 4.317986721860083 5 0.9607768952774974 2.8196177172601082 6 0.5677177971413035 1.9993159707628008 7 0.3729831633075991 1.5324451669680377 8 0.25587579335998184 1.2014808831012302 9 0.19365093740449055 1.022962677554443 >10 0.9655577468298661 10.263550649231897 >50 0.07736239851657542 3.1385786982321267 >100 0.039280984290735906 4.022991493059312 >500 0.0012275079201305363 0.48711658318841305 >1k 3.6825237603916087E-4 0.6125521653997764 >5k 1.227507920130536E-4 0.3632660571566305 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5042 0.3626117249731745 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3968 0.2853715439693686 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2620 0.18842576743945202 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1914 0.13765149575538593 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.595911592355955E-5 0.0 14-15 0.0 0.0 0.0 7.19182318471191E-5 0.0 16-17 0.0 0.0 0.0 7.19182318471191E-5 3.595911592355955E-5 18-19 0.0 0.0 0.0 7.19182318471191E-5 7.19182318471191E-5 20-21 0.0 0.0 0.0 7.19182318471191E-5 7.19182318471191E-5 22-23 7.19182318471191E-5 0.0 0.0 1.438364636942382E-4 7.19182318471191E-5 24-25 7.19182318471191E-5 0.0 0.0 3.2363204331203595E-4 1.0787734777067864E-4 26-27 7.19182318471191E-5 0.0 0.0 4.3150939108271456E-4 1.438364636942382E-4 28-29 7.19182318471191E-5 0.0 0.0 8.630187821654291E-4 1.438364636942382E-4 30-31 7.19182318471191E-5 0.0 0.0 0.001761996680254418 1.438364636942382E-4 32-33 7.19182318471191E-5 0.0 0.0 0.0028407701579612043 1.438364636942382E-4 34-35 7.19182318471191E-5 0.0 0.0 0.003344197780891038 1.438364636942382E-4 36-37 7.19182318471191E-5 0.0 0.0 0.004458930374521384 2.157546955413573E-4 38-39 1.438364636942382E-4 0.0 0.0 0.0064007226343935995 2.157546955413573E-4 40-41 1.438364636942382E-4 0.0 0.0 0.00884594251719565 2.157546955413573E-4 42-43 1.438364636942382E-4 0.0 0.0 0.013736382282799747 2.157546955413573E-4 44-45 2.157546955413573E-4 0.0 0.0 0.022474447452224718 2.157546955413573E-4 46-47 2.876729273884764E-4 0.0 0.0 0.0404540054140045 2.157546955413573E-4 48-49 2.876729273884764E-4 0.0 0.0 0.05300373687132677 2.157546955413573E-4 50-51 2.876729273884764E-4 0.0 0.0 0.0694370528483935 2.157546955413573E-4 52-53 2.876729273884764E-4 0.0 0.0 0.08813579312864446 2.157546955413573E-4 54-55 2.876729273884764E-4 0.0 0.0 0.11107770908787545 2.157546955413573E-4 56-57 2.876729273884764E-4 0.0 0.0 0.13970116536302885 2.157546955413573E-4 58-59 2.876729273884764E-4 0.0 0.0 0.18263634977575893 2.157546955413573E-4 60-61 2.876729273884764E-4 0.0 0.0 0.2331948667642837 2.157546955413573E-4 62-63 2.876729273884764E-4 0.0 0.0 0.29439728206618204 2.157546955413573E-4 64-65 2.876729273884764E-4 0.0 0.0 0.3478325283285915 2.157546955413573E-4 66-67 2.876729273884764E-4 0.0 0.0 0.4274819700992759 2.157546955413573E-4 68-69 2.876729273884764E-4 0.0 0.0 0.5207599168049895 2.157546955413573E-4 70-71 2.876729273884764E-4 0.0 0.0 0.5931815762750383 2.157546955413573E-4 72-73 2.876729273884764E-4 0.0 0.0 0.710192539490301 2.157546955413573E-4 74-75 2.876729273884764E-4 0.0 0.0 0.8156246673781777 2.157546955413573E-4 76-77 2.876729273884764E-4 0.0 0.0 0.9164540284278386 2.157546955413573E-4 78-79 2.876729273884764E-4 0.0 0.0 1.0443246446520165 2.157546955413573E-4 80-81 2.876729273884764E-4 0.0 0.0 1.175647336004856 2.876729273884764E-4 82-83 2.876729273884764E-4 0.0 0.0 1.3397287819640582 3.2363204331203595E-4 84-85 2.876729273884764E-4 0.0 0.0 1.5338720488353563 3.595911592355955E-4 86-87 2.876729273884764E-4 0.0 0.0 1.797020859163965 3.595911592355955E-4 88-89 2.876729273884764E-4 0.0 0.0 2.104651095890017 3.9555027515915506E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3190 0.0 44.57508 1 GTATCAA 5400 0.0 39.190235 1 ATCAACG 6910 0.0 30.407696 3 TATCAAC 7080 0.0 30.013287 2 TCAACGC 7050 0.0 29.876669 4 CAACGCA 7160 0.0 29.351267 5 AACGCAG 7300 0.0 28.698383 6 GTACATG 6030 0.0 25.00078 1 ACGCAGA 8365 0.0 24.989628 7 CGCAGAG 8415 0.0 24.614428 8 TACATGG 6310 0.0 23.655905 2 GCAGAGT 9060 0.0 22.39096 9 ACATGGG 6365 0.0 22.256514 3 GAGTACT 4635 0.0 22.134785 12-13 GTGGTAT 1905 0.0 20.720236 1 TGGTATC 1790 0.0 20.44923 2 GTGTAAG 470 0.0 20.236889 1 AGTACTT 4885 0.0 20.224138 12-13 AGAGTAC 8420 0.0 19.828613 10-11 TAGGGCC 290 2.410161E-9 19.67437 4 >>END_MODULE