FastQCFastQC Report
Thu 26 May 2016
SRR937847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937847_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390468
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56410.40569074584959885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46400.3337005957706326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31820.22884381373753296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39050.042.1135141
GTATCAA60200.034.8973271
ATCAACG77100.026.9378073
TATCAAC78300.026.8284572
TCAACGC78450.026.413674
CAACGCA80000.025.9019055
AACGCAG81950.025.2701136
TAGCGCG701.7814222E-523.74868828-29
ACGCAGA90850.022.6385277
GAGTACT52500.022.25671412-13
CGCAGAG92600.022.1593998
GTACATG56600.022.001451
AGTACTT55750.020.78883712-13
GCAGAGT98400.020.61199
TACATGG59550.020.111942
GTACTTT57800.020.09188314-15
CGCGCGT857.9624166E-519.55774332-33
ACATGGG59600.019.5368713
AGAGTAC90650.019.04679310-11
CCGTCTA1801.2337395E-418.4718679
Produced by FastQC (version 0.11.5)