FastQCFastQC Report
Thu 26 May 2016
SRR937846_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937846_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382914
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51970.3758006643941706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39670.28685804034090334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25410.18374244530028622No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17820.1288583382625384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32100.045.6269451
GTATCAA55700.040.210681
ATCAACG68650.032.26623
TATCAAC69900.031.8943582
TCAACGC69950.031.6054654
CAACGCA71550.030.9629065
AACGCAG73000.030.3291956
GTACATG62400.026.7484251
ACGCAGA82900.026.7072497
CGCAGAG82900.026.6499378
TACATGG63800.025.0343782
TAAGCGT1151.2738776E-424.8056354
GAGTACT44500.024.65741212-13
ACATGGG64650.024.3367143
GCAGAGT89450.024.0079949
GTACTTT50100.021.90010314-15
AGTACTT48350.020.97475612-13
CATGGGA38350.020.579734
GAGTACG1404.8162264E-420.3797511
CAGAGTA86700.020.18932310-11
Produced by FastQC (version 0.11.5)