FastQCFastQC Report
Thu 26 May 2016
SRR937846_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937846_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382914
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55550.4016880297690239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45990.33255864066746016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30220.2185240730804663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37100.044.193311
GTATCAA60500.037.5477451
TATCAAC77100.029.393392
ATCAACG77500.029.180383
TCAACGC79250.028.5360184
CAACGCA80000.028.2091055
AACGCAG82250.027.6037276
ACGCAGA93150.024.0677197
CGCAGAG93800.023.8502988
GTACATG60050.022.5550171
GCAGAGT100050.022.1230289
GAGTACT53550.021.50938812-13
TACATGG61550.021.2270832
ACATGGG62050.020.6731993
ATACCGC1656.325905E-520.1506866
TGTACCG1908.238128E-620.0042425
AGTACTT58750.019.8481212-13
GTACTTT58150.019.8078714-15
AGAGTAC92600.019.79940210-11
TATACCG1200.00475813919.7958645
Produced by FastQC (version 0.11.5)