##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937846_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1382914 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29115331828299 33.0 31.0 34.0 31.0 34.0 2 32.50078240584736 34.0 31.0 34.0 31.0 34.0 3 32.597706726520954 34.0 31.0 34.0 31.0 34.0 4 36.19411040744399 37.0 35.0 37.0 35.0 37.0 5 36.10988246557631 37.0 35.0 37.0 35.0 37.0 6 36.144846317269185 37.0 36.0 37.0 35.0 37.0 7 36.09119656030671 37.0 35.0 37.0 35.0 37.0 8 36.07315133117461 37.0 35.0 37.0 35.0 37.0 9 37.76323835032402 39.0 38.0 39.0 35.0 39.0 10-11 37.69799532002713 39.0 37.0 39.0 35.0 39.0 12-13 37.61271995221684 39.0 37.0 39.0 35.0 39.0 14-15 38.87020306396494 40.0 38.0 41.0 35.0 41.0 16-17 38.83186843144259 40.0 38.0 41.0 35.0 41.0 18-19 38.80590152388363 40.0 38.0 41.0 35.0 41.0 20-21 38.696540782724014 40.0 38.0 41.0 34.0 41.0 22-23 38.544559893095304 40.0 38.0 41.0 34.0 41.0 24-25 38.61886856304875 40.0 38.0 41.0 34.0 41.0 26-27 38.515928683923946 40.0 38.0 41.0 34.0 41.0 28-29 38.37982730668719 40.0 38.0 41.0 34.0 41.0 30-31 38.43780090446694 40.0 38.0 41.0 34.0 41.0 32-33 38.3930699956758 40.0 38.0 41.0 34.0 41.0 34-35 38.27696371574805 40.0 38.0 41.0 34.0 41.0 36-37 38.13178223663944 40.0 38.0 41.0 34.0 41.0 38-39 38.002993678565694 40.0 38.0 41.0 33.0 41.0 40-41 37.82740177624928 40.0 37.0 41.0 33.0 41.0 42-43 37.62705164601704 40.0 37.0 41.0 33.0 41.0 44-45 37.33368018546345 40.0 37.0 41.0 32.5 41.0 46-47 37.01475832915134 40.0 37.0 41.0 31.5 41.0 48-49 36.71774492123154 40.0 36.0 41.0 31.0 41.0 50-51 36.486677045716505 39.0 36.0 41.0 30.5 41.0 52-53 36.25127918294268 39.0 36.0 41.0 30.0 41.0 54-55 36.028802224867206 39.0 35.0 40.0 29.5 41.0 56-57 35.78412648942739 39.0 35.0 40.0 29.0 41.0 58-59 35.519220284124685 38.0 35.0 40.0 28.0 41.0 60-61 35.18283819528908 38.0 34.5 40.0 28.0 41.0 62-63 34.841023013723195 38.0 34.0 40.0 27.5 41.0 64-65 34.45119291582846 37.0 34.0 40.0 26.5 41.0 66-67 34.03555535629837 36.5 33.5 39.0 26.0 41.0 68-69 33.60444141862762 36.0 33.0 39.0 26.0 40.0 70-71 33.19086110922299 35.5 32.5 38.5 25.0 40.0 72-73 32.70540033581264 35.0 32.0 38.0 24.0 40.0 74-75 32.20592278334011 35.0 31.5 37.0 22.5 39.0 76-77 30.959112424923028 34.0 30.5 36.0 21.5 38.5 78-79 31.560125213860008 34.5 31.0 36.0 22.0 38.5 80-81 31.356935066099553 34.5 31.0 36.0 21.5 37.5 82-83 31.007195675219137 34.0 31.0 35.0 20.0 37.0 84-85 30.631142283612718 34.0 31.0 35.0 19.5 36.5 86-87 30.207698020267348 34.0 30.5 35.0 18.0 36.0 88-89 29.857559833800224 34.0 30.0 35.0 13.5 36.0 90-91 29.349857619490436 34.0 29.5 35.0 7.0 35.0 92-93 28.89566162465634 34.0 29.0 35.0 2.0 35.0 94-95 28.448030390899216 34.0 29.0 35.0 2.0 35.0 96-97 27.925526099236826 33.0 28.5 35.0 2.0 35.0 98-99 27.06997723647313 33.0 26.5 35.0 2.0 35.0 100-101 24.43683048982077 31.0 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23274880091181416 1101 2 0.1971573053101352 1101 3 0.20659994974679563 1101 4 0.17516358902472717 1101 5 0.18325349156921789 1101 6 0.20682183442776392 1101 7 0.22178566902810104 1101 8 0.2191269810574994 1101 9 0.35236017943576314 1101 10-11 0.27274307400424647 1101 12-13 0.2287584911050473 1101 14-15 0.29224442743879564 1101 16-17 0.26889469377728403 1101 18-19 0.37143887700994327 1101 20-21 0.4777802185709774 1101 22-23 0.47207140993540975 1101 24-25 0.3465527933015764 1101 26-27 0.3880734949655107 1101 28-29 0.25862480308034463 1101 30-31 0.35565668292451136 1101 32-33 0.3442666576188458 1101 34-35 0.4204667870965082 1101 36-37 0.3566525833307068 1101 38-39 0.23324803630959678 1101 40-41 0.26168928354441334 1101 42-43 0.4178955506680282 1101 44-45 0.39470758621719426 1101 46-47 0.32300571452526583 1101 48-49 0.22975142648343194 1101 50-51 0.21902964346003273 1101 52-53 0.08041004838877086 1101 54-55 0.3083098992271047 1101 56-57 0.12782722343717978 1101 58-59 0.22592265713734605 1101 60-61 0.13196533376010677 1101 62-63 0.1742042599168201 1101 64-65 0.2675445721200873 1101 66-67 0.09144007937638321 1101 68-69 0.09253961018897172 1101 70-71 0.07342681543382668 1101 72-73 0.23648032104077288 1101 74-75 0.2084821468279614 1101 76-77 0.21076622913581744 1101 78-79 0.15410706244876238 1101 80-81 0.056548094165673746 1101 82-83 0.18005924658955763 1101 84-85 0.10137407254235242 1101 86-87 -0.18224830285929272 1101 88-89 0.22790435155016908 1101 90-91 0.1711084666488354 1101 92-93 0.22212290165661486 1101 94-95 0.2890392748927866 1101 96-97 0.3797316536377622 1101 98-99 0.23913207717746587 1101 100-101 0.7256189989791189 1102 1 0.35288932169322607 1102 2 0.358267229590318 1102 3 0.32452347036205254 1102 4 0.1784867156121095 1102 5 0.09501973335041214 1102 6 0.1871061991171672 1102 7 0.25987344704324755 1102 8 0.23098579565077415 1102 9 0.2253028080288928 1102 10-11 0.291065369809985 1102 12-13 0.25608645996260293 1102 14-15 0.28828753313795374 1102 16-17 0.3772642254537999 1102 18-19 0.24895792564627328 1102 20-21 0.3356405997810654 1102 22-23 0.34409305773147025 1102 24-25 0.2831594063234846 1102 26-27 0.3391974238505995 1102 28-29 0.30440087182159203 1102 30-31 0.3696755297995864 1102 32-33 0.3793422587487143 1102 34-35 0.265008551676722 1102 36-37 0.33565754428396843 1102 38-39 0.4734388787221775 1102 40-41 0.44669486164975325 1102 42-43 0.5534719944478468 1102 44-45 0.5745599444502432 1102 46-47 0.6995159568761409 1102 48-49 0.7104203243922385 1102 50-51 0.649187017240564 1102 52-53 0.6661894402566091 1102 54-55 0.6377005802306854 1102 56-57 0.6358786844260536 1102 58-59 0.6944075487796795 1102 60-61 0.5376241436060525 1102 62-63 0.5617923821129338 1102 64-65 0.7481185235549717 1102 66-67 0.5947465903308284 1102 68-69 0.5897054579423013 1102 70-71 0.6572075840255351 1102 72-73 0.580400186216778 1102 74-75 0.6127547381545995 1102 76-77 0.4556972742591263 1102 78-79 0.4660180976026993 1102 80-81 0.503489630874359 1102 82-83 0.5305019345242385 1102 84-85 0.6141514942708532 1102 86-87 0.5617926190709674 1102 88-89 0.6330646924080128 1102 90-91 0.5704462149932077 1102 92-93 0.7109975485744151 1102 94-95 0.5833543547665094 1102 96-97 0.709703881794141 1102 98-99 0.6141825174917379 1102 100-101 0.9987301575634646 1103 1 0.15650846889898418 1103 2 0.17227520759271897 1103 3 0.1394899704975998 1103 4 0.08261029719299273 1103 5 0.05647552514778198 1103 6 0.0775490557067684 1103 7 0.09318257527220908 1103 8 0.03461462489828904 1103 9 0.05009173951876278 1103 10-11 0.07888342488143962 1103 12-13 0.04142591234631965 1103 14-15 0.18759671945916523 1103 16-17 0.18452382035717818 1103 18-19 0.055036311456277076 1103 20-21 0.11657714367944294 1103 22-23 0.1384150243477862 1103 24-25 0.1650917482157368 1103 26-27 0.09937324972876382 1103 28-29 0.08686475780586278 1103 30-31 0.0662112952618088 1103 32-33 0.15905160385540285 1103 34-35 0.1694976303336304 1103 36-37 0.1696831436023487 1103 38-39 0.061146074415610485 1103 40-41 0.1355503474946076 1103 42-43 0.07839356990967872 1103 44-45 0.17764948397233837 1103 46-47 0.10279962133530063 1103 48-49 -0.08216613624058056 1103 50-51 -0.04938197698893276 1103 52-53 -0.23817135134712686 1103 54-55 -0.05820585251785104 1103 56-57 -0.024068645266837052 1103 58-59 0.04098349471515661 1103 60-61 0.00474877084744918 1103 62-63 0.08770878736446974 1103 64-65 0.21469369492710655 1103 66-67 0.24987952476404018 1103 68-69 0.04053762943062367 1103 70-71 0.03241332741269787 1103 72-73 0.048827048073405876 1103 74-75 0.05318597629409538 1103 76-77 0.11081810614015453 1103 78-79 0.25263800000771397 1103 80-81 0.013086954758019687 1103 82-83 -0.02137444516671394 1103 84-85 0.10586850755461441 1103 86-87 0.2064495843318035 1103 88-89 0.11621551925437856 1103 90-91 0.29640086430965695 1103 92-93 0.19839153021726474 1103 94-95 0.43449296287618466 1103 96-97 0.3673589881029571 1103 98-99 0.3455978383545997 1103 100-101 0.2702049261626662 1104 1 0.24135095144944785 1104 2 0.306885927941984 1104 3 0.1781821310064018 1104 4 0.12549641032529024 1104 5 0.11571636919338602 1104 6 0.13273069259468429 1104 7 0.16249227425083745 1104 8 0.17698668407849283 1104 9 0.19153068634820158 1104 10-11 0.2476790699079885 1104 12-13 0.30638265905179907 1104 14-15 0.30010413045977913 1104 16-17 0.3181778478991504 1104 18-19 0.3640144018435336 1104 20-21 0.28236291186334483 1104 22-23 0.40233254664816087 1104 24-25 0.26915904010136416 1104 26-27 0.33497569670641525 1104 28-29 0.27334574522063093 1104 30-31 0.42513952982671555 1104 32-33 0.3358949218277516 1104 34-35 0.44358506666640096 1104 36-37 0.41873655670499943 1104 38-39 0.3167093778354442 1104 40-41 0.36611836700575395 1104 42-43 0.3775217667458506 1104 44-45 0.21280794464013297 1104 46-47 0.3861137534397585 1104 48-49 0.16994599893607187 1104 50-51 0.33561950521119144 1104 52-53 0.14523339708308924 1104 54-55 0.14422899804942801 1104 56-57 0.0687642434064557 1104 58-59 0.2584879413155292 1104 60-61 0.24614864149179994 1104 62-63 0.3543885917140557 1104 64-65 0.3068174855865493 1104 66-67 0.2564938428172425 1104 68-69 0.4058780638500039 1104 70-71 0.316872795976586 1104 72-73 0.29626014694962777 1104 74-75 0.3339045738632862 1104 76-77 0.22722808269444172 1104 78-79 0.3965545790898304 1104 80-81 0.2380125078881541 1104 82-83 0.42255796650763244 1104 84-85 0.5698072522658535 1104 86-87 0.5418428389737855 1104 88-89 0.5108024570289196 1104 90-91 0.6331668383846285 1104 92-93 0.7220460967410993 1104 94-95 0.8003808007504425 1104 96-97 1.0372073320811808 1104 98-99 1.153494596378696 1104 100-101 1.3513486456765058 1105 1 0.28507040403987105 1105 2 0.256166754642166 1105 3 0.2254415919755388 1105 4 0.09946303509836696 1105 5 0.122663722914929 1105 6 0.11548100679661388 1105 7 0.15392154390591628 1105 8 0.2260021847068998 1105 9 0.4356446369137643 1105 10-11 0.23574812450929983 1105 12-13 0.33993069703532086 1105 14-15 0.43025355093178774 1105 16-17 0.39183376187764907 1105 18-19 0.39080186169550757 1105 20-21 0.41160638344552325 1105 22-23 0.33242087230100026 1105 24-25 0.413264262835618 1105 26-27 0.26407952298766446 1105 28-29 0.45052770304776146 1105 30-31 0.4453846073298635 1105 32-33 0.5048849371887414 1105 34-35 0.48068184085995114 1105 36-37 0.5713112669410023 1105 38-39 0.6423063614969422 1105 40-41 0.5927539625929583 1105 42-43 0.6660464141414693 1105 44-45 0.47459924473788107 1105 46-47 0.452042045577727 1105 48-49 0.3352581061836588 1105 50-51 0.4241493893055903 1105 52-53 0.48163194477195503 1105 54-55 0.4433823984125027 1105 56-57 0.4063340660569281 1105 58-59 0.6036230481441862 1105 60-61 0.6270573832876423 1105 62-63 0.6942677985287489 1105 64-65 0.6337061882817068 1105 66-67 0.6781091898358156 1105 68-69 0.46026118073312006 1105 70-71 0.34239472028883 1105 72-73 0.40173773387883216 1105 74-75 0.36234333939882646 1105 76-77 0.38633644744797024 1105 78-79 0.4984048793272251 1105 80-81 0.43387377028167506 1105 82-83 0.46991343362116567 1105 84-85 0.53657690734196 1105 86-87 0.7461159851824171 1105 88-89 0.6123944134595867 1105 90-91 0.7400478605882306 1105 92-93 0.7991756875803695 1105 94-95 0.7964480399628435 1105 96-97 0.6155780205325172 1105 98-99 0.6045890664247757 1105 100-101 1.1796879139351297 1106 1 0.16531971870238493 1106 2 0.131761331214733 1106 3 0.20404980120721916 1106 4 0.03694729085890458 1106 5 0.07737588117232264 1106 6 0.10165756455018737 1106 7 0.12253493947530103 1106 8 0.07422771630651681 1106 9 0.13437768870305433 1106 10-11 0.06535377761738914 1106 12-13 -0.0851665467966427 1106 14-15 0.13283560620332935 1106 16-17 0.3059331913805039 1106 18-19 0.2853691729498635 1106 20-21 0.2456834005604449 1106 22-23 0.26128191549578617 1106 24-25 0.3806134885894963 1106 26-27 0.33158989566307895 1106 28-29 0.3437743504508788 1106 30-31 0.3771787097229833 1106 32-33 0.27182666498010377 1106 34-35 0.16095893947446172 1106 36-37 0.13553016743740187 1106 38-39 0.2337563133380982 1106 40-41 0.1182272159085116 1106 42-43 -0.14282778012391617 1106 44-45 -0.1011166550917082 1106 46-47 -0.06045424989534354 1106 48-49 -0.025422044961040058 1106 50-51 -0.0054544115390413594 1106 52-53 -0.04101382836281431 1106 54-55 -0.05993593158675736 1106 56-57 -0.2187668823019706 1106 58-59 -0.011677574216342634 1106 60-61 -0.03862295308341146 1106 62-63 -0.08897106936782251 1106 64-65 0.08251333060962907 1106 66-67 0.015892586146108556 1106 68-69 -0.11173166952989533 1106 70-71 0.031050532691587307 1106 72-73 0.11828400342020728 1106 74-75 9.815552986580656E-4 1106 76-77 0.014096320012246366 1106 78-79 -0.08172033368307297 1106 80-81 0.06171236404325242 1106 82-83 0.02630281644697874 1106 84-85 0.18684085488585822 1106 86-87 0.2490536750394199 1106 88-89 -0.10880288964480656 1106 90-91 0.10639779020221951 1106 92-93 0.2372897129193312 1106 94-95 0.27277703899966355 1106 96-97 0.622071130112424 1106 98-99 0.8670075668322745 1106 100-101 0.626654832509626 1107 1 0.3143967195463091 1107 2 0.3225188290687697 1107 3 0.2548391612799392 1107 4 0.11320762384295335 1107 5 0.09107677983730156 1107 6 0.06473687515185844 1107 7 0.11274558735728846 1107 8 0.1457542752664409 1107 9 0.2563378153709195 1107 10-11 0.22002691507690741 1107 12-13 0.24441397255896646 1107 14-15 0.37089107076185 1107 16-17 0.2898816766957495 1107 18-19 0.36860361480782444 1107 20-21 0.4133773092071351 1107 22-23 0.3567886628955037 1107 24-25 0.32765719392110526 1107 26-27 0.28718491138808844 1107 28-29 0.4325919121305972 1107 30-31 0.3044842550941027 1107 32-33 0.32590312707338853 1107 34-35 0.3280938491415242 1107 36-37 0.27130689760433313 1107 38-39 0.2915736573480956 1107 40-41 0.4243446028016109 1107 42-43 0.5537174904771476 1107 44-45 0.37140055419447293 1107 46-47 0.24752381536073642 1107 48-49 0.24358807848879138 1107 50-51 0.27380824494270684 1107 52-53 0.2957280970668634 1107 54-55 0.4963222897840751 1107 56-57 0.31437062059968923 1107 58-59 0.24371372868517938 1107 60-61 0.49625120706220827 1107 62-63 0.46411467008369556 1107 64-65 0.6061229020027668 1107 66-67 0.5370249893572066 1107 68-69 0.32919956636405345 1107 70-71 0.32266178759005726 1107 72-73 0.6273346524881731 1107 74-75 0.49170528180425066 1107 76-77 0.44178332975820567 1107 78-79 0.588834015408537 1107 80-81 0.5868022341573429 1107 82-83 0.44180212981110145 1107 84-85 0.5259449668187166 1107 86-87 0.9786171279320044 1107 88-89 0.8498174459136756 1107 90-91 0.9656367127298786 1107 92-93 1.0327315209809242 1107 94-95 1.3504876597633313 1107 96-97 1.4667829491442745 1107 98-99 1.621362949891207 1107 100-101 1.7784722300331097 1108 1 0.16642292002541126 1108 2 0.10406778327713795 1108 3 0.15142852553127994 1108 4 0.08187198700272802 1108 5 -0.011128326014734569 1108 6 0.022883008464667398 1108 7 0.09550480773688008 1108 8 0.07073390558540638 1108 9 0.12394043897104012 1108 10-11 0.16497734330273772 1108 12-13 0.2111323928929778 1108 14-15 0.16621407358955764 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82-83 0.13787382387383929 2316 84-85 0.37442534723045995 2316 86-87 0.4098557567856851 2316 88-89 0.337239301509225 2316 90-91 0.6995951135331104 2316 92-93 0.46484649056571214 2316 94-95 0.43433244783983227 2316 96-97 0.3767089889706057 2316 98-99 0.6214906490162697 2316 100-101 1.2147578267127557 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 9.0 5 21.0 6 53.0 7 124.0 8 293.0 9 552.0 10 924.0 11 1553.0 12 2513.0 13 3664.0 14 4725.0 15 6546.0 16 9009.0 17 12720.0 18 11049.0 19 6644.0 20 5677.0 21 5817.0 22 6201.0 23 6844.0 24 8153.0 25 9578.0 26 11811.0 27 14499.0 28 18340.0 29 23248.0 30 29331.0 31 37191.0 32 48933.0 33 64851.0 34 90761.0 35 132573.0 36 207693.0 37 318996.0 38 258351.0 39 23662.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7394967586361 19.62690502004561 15.081256504968549 22.552341716349737 2 15.26877304011674 20.579876984396716 40.13713072541026 24.014219250076287 3 19.340248200538863 24.53905304306703 30.067668705356947 26.053030051037158 4 13.179281704633624 17.04351611417349 36.62095880218062 33.156243379012274 5 13.876036889357243 37.65601708638882 33.87717964648302 14.590766377770914 6 32.307721232122894 36.25699067331736 16.9887643049387 14.446523789621047 7 28.87793456426068 33.080075839856995 20.18614317303896 17.855846422843356 8 26.608668362602444 35.513488185093216 19.372065074183933 18.505778378120404 9 26.501214102973865 16.977628399162928 20.227071242318758 36.29408625554445 10-11 25.07719207412753 27.03353209237885 26.99567724384886 20.893598589644764 12-13 25.78531736117313 24.29026849702194 28.01572388328719 21.908690258517733 14-15 22.526599629478046 25.735837514118735 26.258465819277266 25.479097037125953 16-17 21.988315976264612 29.3330243239999 26.66615566839297 22.012504031342512 18-19 21.832738695247862 27.40416251480569 29.505016219374454 21.258082570571997 20-21 22.56134510171999 26.598038634361938 29.640816420977735 21.199799842940344 22-23 22.346257015598738 26.658208598521732 29.429340722633334 21.5661936632462 24-25 22.237471830306088 26.75160087742292 29.704316285870373 21.306611006400615 26-27 22.043331877464013 27.079784049264305 29.40625840619911 21.470625667072575 28-29 21.794554271981244 26.957470909873848 29.71309215666637 21.534882661478537 30-31 21.88097018325073 26.71482102285464 29.772711824451846 21.631496969442786 32-33 21.714293151996436 27.034002114375877 29.55350079614495 21.698203937482734 34-35 22.256409292262568 26.880847254420736 29.327022504653215 21.535720948663474 36-37 22.31248653206201 26.660949270887418 29.191149991973468 21.835414205077104 38-39 22.159725275360977 26.926225240651608 29.33335599083673 21.580693493150687 40-41 22.187568921943303 26.872200187286904 29.26791789746946 21.672312993300334 42-43 22.163468840176176 26.914103463488363 29.04864360567292 21.87378409066254 44-45 22.10383292091916 27.263445160002718 28.811227596220736 21.821494322857387 46-47 22.311980354526746 27.332393771413116 28.333685247238797 22.021940626821333 48-49 22.593053508750362 27.465265374419523 28.092166251842126 21.84951486498799 50-51 22.5077264385204 27.63689571441174 27.855492098568675 21.999885748499185 52-53 22.684997042476972 27.747531661404828 27.659348303654458 21.90812299246374 54-55 22.3905101835689 27.609055949972305 27.894612390936818 22.105821475521978 56-57 22.367117550332125 28.053045959473984 27.760764588398125 21.819071901795773 58-59 22.52739505131917 27.57087570159822 27.814130162830082 22.087599084252528 60-61 22.491203357547903 27.482041544159653 28.155510754826402 21.871244343466042 62-63 22.479669740851563 27.605512707225465 28.14513411535352 21.769683436569448 64-65 22.45663866299712 27.496431448376402 27.960560092673877 22.086369795952603 66-67 22.217614399738522 27.939011391886986 27.83397955332002 22.00939465505447 68-69 22.323285095767087 27.701065685645332 27.949888369654623 22.02576084893296 70-71 22.242417098966385 27.798040948316384 27.83296719824949 22.12657475446774 72-73 22.34336336171302 27.730177003052976 27.71199076732176 22.21446886791225 74-75 22.30641240163886 27.9334434389991 27.493611316394222 22.26653284296782 76-77 22.358548687770895 27.877293888123194 27.663831590395354 22.100325833710556 78-79 22.3944511372363 27.79305148404022 27.583168584597452 22.229328794126026 80-81 22.145030092276233 28.113084481814838 27.615583915980253 22.12630150992868 82-83 22.172454998888192 28.01463609525755 27.630438594746842 22.182470311107416 84-85 22.29895679784338 27.878802998469528 27.413622984361545 22.408617219325542 86-87 22.29842202768936 27.645139177128875 27.71647405406265 22.339964741119115 88-89 22.34679813784516 27.627242185703523 27.60764588398122 22.418313792470105 90-91 22.085648434409507 27.86947783758531 27.720031328443145 22.32484239956204 92-93 22.162049979680116 27.776484183032245 27.63185868353193 22.429607153755708 94-95 22.104556031683824 27.617263257151205 27.643982199905416 22.634198511259555 96-97 22.158753183495143 27.608043594901783 27.616070124389513 22.617133097213564 98-99 22.040296711529173 27.792408951675185 27.350834338473284 22.816459998322355 100-101 21.903528600880115 27.779599951831923 27.690460081068462 22.626411366219497 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 311.0 1 214.0 2 155.5 3 202.0 4 180.0 5 170.0 6 314.5 7 673.0 8 1093.5 9 1365.0 10 1367.0 11 1329.5 12 1477.5 13 1643.5 14 1823.5 15 1944.0 16 2025.0 17 2087.5 18 2174.0 19 2369.5 20 2542.0 21 2809.5 22 3344.0 23 4000.5 24 4819.5 25 6103.0 26 7725.5 27 9376.5 28 10970.5 29 12963.5 30 15520.5 31 18168.5 32 20901.0 33 23830.0 34 27243.5 35 29783.5 36 32355.0 37 35626.0 38 37980.0 39 39896.0 40 41908.0 41 45351.0 42 49601.5 43 52829.5 44 54930.5 45 56252.5 46 55708.5 47 55061.0 48 57770.5 49 57735.5 50 54547.0 51 52006.0 52 48996.0 53 46441.5 54 43508.0 55 39388.5 56 34892.5 57 30663.0 58 27374.0 59 24324.5 60 20383.0 61 16116.5 62 12193.0 63 9171.0 64 6755.0 65 5113.0 66 3928.5 67 3040.5 68 2250.0 69 1483.5 70 1093.0 71 810.0 72 653.5 73 548.0 74 420.5 75 325.0 76 224.0 77 140.0 78 88.5 79 61.0 80 38.5 81 25.5 82 17.0 83 11.0 84 5.5 85 1.5 86 2.0 87 2.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02292261123974448 2 0.0 3 0.0 4 7.231107646607092E-5 5 0.02118714540455878 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0035793982850705107 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.761995322919573E-4 24-25 3.615553823303546E-5 26-27 4.3386645879642553E-4 28-29 9.761995322919573E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.4462215293214184E-4 40-41 6.507996881946383E-4 42-43 0.014751459599078467 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.0846661469910638E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.231107646607092E-5 82-83 0.002277798908681234 84-85 6.146441499616028E-4 86-87 0.0 88-89 0.0 90-91 0.010485106087580283 92-93 0.0023139544469142695 94-95 0.0 96-97 0.0 98-99 0.0015908436822535603 100-101 0.018475480037081122 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1382914.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.71746375891381 #Duplication Level Percentage of deduplicated Percentage of total 1 76.0133847467612 43.112863945668 2 13.683882045761026 15.522301680234044 3 4.549387972322534 7.740892423363251 4 1.9835368449705226 4.500047164763434 5 1.0592241516011454 3.0038253715502097 6 0.6267701067641644 2.1329286489340222 7 0.39927310889678647 1.585203065863363 8 0.28394408117127395 1.2883670506711848 9 0.21090038480247453 1.0765561438597793 >10 1.0627294633414885 10.855878838942361 >50 0.08058174299933074 3.1735909063574415 >100 0.044335198440904916 4.374472822647746 >500 0.001409479614929141 0.5167167225803211 >1k 5.125380417924148E-4 0.7126475642225919 >5k 1.281345104481037E-4 0.4037076503422902 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5555 0.4016880297690239 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4599 0.33255864066746016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3022 0.2185240730804663 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 7.231107646607092E-5 0.0 0.0 0.0 0.0 8 7.231107646607092E-5 0.0 0.0 0.0 0.0 9 7.231107646607092E-5 0.0 0.0 0.0 0.0 10-11 7.231107646607092E-5 0.0 0.0 0.0 0.0 12-13 7.231107646607092E-5 0.0 0.0 0.0 0.0 14-15 7.231107646607092E-5 0.0 0.0 3.615553823303546E-5 0.0 16-17 7.231107646607092E-5 0.0 0.0 7.231107646607092E-5 0.0 18-19 7.231107646607092E-5 0.0 0.0 7.231107646607092E-5 0.0 20-21 7.231107646607092E-5 0.0 0.0 7.231107646607092E-5 7.231107646607092E-5 22-23 7.231107646607092E-5 0.0 0.0 1.4462215293214184E-4 7.231107646607092E-5 24-25 7.231107646607092E-5 0.0 0.0 2.1693322939821276E-4 7.231107646607092E-5 26-27 7.231107646607092E-5 0.0 0.0 2.892443058642837E-4 7.231107646607092E-5 28-29 1.0846661469910638E-4 0.0 0.0 9.038884558258865E-4 7.231107646607092E-5 30-31 1.4462215293214184E-4 0.0 0.0 0.0023139544469142695 7.231107646607092E-5 32-33 1.4462215293214184E-4 0.0 0.0 0.0033263095174392624 7.231107646607092E-5 34-35 1.4462215293214184E-4 0.0 0.0 0.004230197973265149 7.231107646607092E-5 36-37 2.1693322939821276E-4 0.0 0.0 0.005314864120256213 7.231107646607092E-5 38-39 2.1693322939821276E-4 0.0 0.0 0.007592663028937447 7.231107646607092E-5 40-41 2.1693322939821276E-4 0.0 0.0 0.010665883778745461 7.231107646607092E-5 42-43 2.1693322939821276E-4 0.0 0.0 0.015474570363739176 7.231107646607092E-5 44-45 2.1693322939821276E-4 0.0 0.0 0.026321231833649816 7.231107646607092E-5 46-47 2.1693322939821276E-4 0.0 0.0 0.04783377708230591 7.231107646607092E-5 48-49 2.892443058642837E-4 0.0 0.0 0.0646461023606674 7.231107646607092E-5 50-51 2.892443058642837E-4 0.0 0.0 0.08424240408297262 7.231107646607092E-5 52-53 2.892443058642837E-4 0.0 0.0 0.10647806009628943 1.0846661469910638E-4 54-55 2.892443058642837E-4 0.0 0.0 0.13167847024471513 1.4462215293214184E-4 56-57 2.892443058642837E-4 0.0 0.0 0.16295301081629082 1.4462215293214184E-4 58-59 2.892443058642837E-4 0.0 0.0 0.21176298743088867 1.4462215293214184E-4 60-61 2.892443058642837E-4 0.0 0.0 0.26747867184799634 1.4462215293214184E-4 62-63 2.892443058642837E-4 0.0 0.0 0.3367526831024923 1.4462215293214184E-4 64-65 2.892443058642837E-4 0.0 0.0 0.39915714209271147 1.4462215293214184E-4 66-67 2.892443058642837E-4 0.0 0.0 0.4879912995312796 1.4462215293214184E-4 68-69 2.892443058642837E-4 0.0 0.0 0.5932762268658789 1.4462215293214184E-4 70-71 2.892443058642837E-4 0.0 0.0 0.6739030771255479 1.4462215293214184E-4 72-73 2.892443058642837E-4 0.0 0.0 0.8025083266204551 1.4462215293214184E-4 74-75 2.892443058642837E-4 0.0 0.0 0.914698961757564 1.4462215293214184E-4 76-77 2.892443058642837E-4 0.0 0.0 1.026672663665275 1.4462215293214184E-4 78-79 2.892443058642837E-4 0.0 0.0 1.163521375877314 1.4462215293214184E-4 80-81 2.892443058642837E-4 0.0 0.0 1.2961760456543212 1.4462215293214184E-4 82-83 2.892443058642837E-4 0.0 0.0 1.4572851240207272 1.4462215293214184E-4 84-85 2.892443058642837E-4 0.0 0.0 1.6617085371903098 1.4462215293214184E-4 86-87 2.892443058642837E-4 0.0 0.0 1.9294041422677042 1.4462215293214184E-4 88-89 2.892443058642837E-4 0.0 0.0 2.2649275370702737 1.4462215293214184E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3710 0.0 44.19331 1 GTATCAA 6050 0.0 37.547745 1 TATCAAC 7710 0.0 29.39339 2 ATCAACG 7750 0.0 29.18038 3 TCAACGC 7925 0.0 28.536018 4 CAACGCA 8000 0.0 28.209105 5 AACGCAG 8225 0.0 27.603727 6 ACGCAGA 9315 0.0 24.067719 7 CGCAGAG 9380 0.0 23.850298 8 GTACATG 6005 0.0 22.555017 1 GCAGAGT 10005 0.0 22.123028 9 GAGTACT 5355 0.0 21.509388 12-13 TACATGG 6155 0.0 21.227083 2 ACATGGG 6205 0.0 20.673199 3 ATACCGC 165 6.325905E-5 20.150686 6 TGTACCG 190 8.238128E-6 20.004242 5 AGTACTT 5875 0.0 19.84812 12-13 GTACTTT 5815 0.0 19.80787 14-15 AGAGTAC 9260 0.0 19.799402 10-11 TATACCG 120 0.004758139 19.795864 5 >>END_MODULE