FastQCFastQC Report
Thu 26 May 2016
SRR937845_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937845_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300571
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49400.3798331655865001No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39390.3028669715071303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25770.19814373840413174No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18390.1413994314804805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29250.051.528771
GTATCAA49850.041.5850941
ATCAACG63750.031.6828443
TATCAAC64600.031.5626662
TCAACGC64550.031.2889774
CAACGCA65850.030.7434435
AACGCAG67100.030.1579386
GGATCGT1056.9042406E-527.1441696
GTACATG55550.026.619061
ACGCAGA76500.026.1417877
CGCAGAG77650.025.57118
TACATGG57250.025.0715052
ACATGGG57000.024.5958923
GCAGAGT81950.023.5899269
GTGGTAT14550.023.201271
GAGTACT45650.023.15013912-13
CATGGGG27250.023.0200424
GTATTCG1050.00218398122.6410791
GTCTAGG4050.022.3056551
TATGACG1100.002869690321.6011074
Produced by FastQC (version 0.11.5)