##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937845_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1300571 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81775543203716 33.0 31.0 34.0 30.0 34.0 2 31.94666342706396 33.0 31.0 34.0 30.0 34.0 3 31.975475387349096 34.0 31.0 34.0 30.0 34.0 4 35.55394592067638 37.0 35.0 37.0 33.0 37.0 5 35.546009406637545 37.0 35.0 37.0 33.0 37.0 6 35.597749757606465 37.0 35.0 37.0 33.0 37.0 7 35.54125841649552 37.0 35.0 37.0 33.0 37.0 8 35.52267119595931 37.0 35.0 37.0 33.0 37.0 9 37.15893019296909 39.0 37.0 39.0 34.0 39.0 10-11 37.04377461899428 39.0 37.0 39.0 33.0 39.0 12-13 36.93032098978064 39.0 37.0 39.0 33.0 39.0 14-15 38.11936795453689 40.0 38.0 41.0 33.0 41.0 16-17 38.05437265631788 40.0 38.0 41.0 33.0 41.0 18-19 37.98131090113496 40.0 38.0 41.0 33.0 41.0 20-21 37.89904703395662 40.0 38.0 41.0 33.0 41.0 22-23 37.69926939782603 40.0 37.5 41.0 32.5 41.0 24-25 37.53565587730312 40.0 37.0 41.0 32.0 41.0 26-27 37.4928450657442 40.0 37.0 41.0 32.0 41.0 28-29 37.54495333203647 40.0 37.0 41.0 32.0 41.0 30-31 37.36793339233306 40.0 37.0 41.0 32.0 41.0 32-33 37.15595188574865 39.5 36.5 41.0 31.0 41.0 34-35 37.1938144860988 40.0 37.0 41.0 31.0 41.0 36-37 37.1221959431665 40.0 37.0 41.0 31.0 41.0 38-39 37.002722265835544 40.0 36.5 41.0 31.0 41.0 40-41 36.76673361162136 39.5 36.0 41.0 30.5 41.0 42-43 36.53009562722835 39.0 36.0 41.0 30.0 41.0 44-45 36.31414432583842 39.0 36.0 41.0 30.0 41.0 46-47 35.98331425197087 39.0 35.0 41.0 28.5 41.0 48-49 35.648198752701695 39.0 35.0 40.5 27.5 41.0 50-51 34.73235448122402 38.0 34.0 39.5 25.5 40.5 52-53 34.84240422091527 38.0 34.5 39.5 26.0 40.5 54-55 35.19053592614321 39.0 35.0 40.0 26.0 41.0 56-57 35.00874577397158 38.5 35.0 40.0 26.0 41.0 58-59 34.774503660315354 38.0 34.0 40.0 26.0 41.0 60-61 34.46370940148596 38.0 34.0 40.0 25.0 41.0 62-63 34.11039920158146 38.0 34.0 40.0 23.5 41.0 64-65 33.738125023547354 37.0 33.0 40.0 22.5 41.0 66-67 33.34262027986169 36.5 33.0 39.0 22.0 41.0 68-69 32.868323990001315 36.0 32.0 39.0 20.5 40.5 70-71 32.42603248880684 35.5 32.0 39.0 19.5 40.0 72-73 31.96391661816233 35.0 31.0 38.0 18.0 40.0 74-75 31.50740674672894 35.0 31.0 37.0 15.0 39.0 76-77 31.00999176515546 35.0 31.0 37.0 9.5 39.0 78-79 30.482256255137166 34.5 30.0 36.0 7.0 39.0 80-81 29.95924636179032 34.0 29.5 36.0 4.5 37.5 82-83 29.43685119843515 34.0 29.0 35.0 2.0 37.0 84-85 28.974524651095557 34.0 29.0 35.0 2.0 36.5 86-87 28.502862973263284 34.0 28.5 35.0 2.0 36.0 88-89 28.02532233918794 34.0 27.0 35.0 2.0 36.0 90-91 27.53419267383326 33.0 26.5 35.0 2.0 35.0 92-93 26.97675905429231 33.0 25.5 35.0 2.0 35.0 94-95 26.34766460270143 32.5 25.0 35.0 2.0 35.0 96-97 25.622369328548768 32.0 22.0 34.0 2.0 35.0 98-99 24.676678551190207 32.0 10.5 34.0 2.0 35.0 100-101 22.291291671119836 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18435531998228072 1101 2 0.23963297607023648 1101 3 0.17243439726107468 1101 4 0.14631452468578487 1101 5 0.1680907433227503 1101 6 0.08590942032284232 1101 7 0.22840723223699655 1101 8 0.14549793775280762 1101 9 0.1557895610050366 1101 10-11 0.18438235114371793 1101 12-13 0.31047379360519756 1101 14-15 0.42495214318932995 1101 16-17 0.6149022844011611 1101 18-19 0.584180830240328 1101 20-21 0.6767342682626492 1101 22-23 0.48246889314612673 1101 24-25 0.5356860924567073 1101 26-27 0.7314603220991458 1101 28-29 0.6683172959294978 1101 30-31 0.7214032827884793 1101 32-33 0.6319910078697717 1101 34-35 0.7250323201915805 1101 36-37 0.5374331243168058 1101 38-39 0.5876610472570718 1101 40-41 0.6945771701559877 1101 42-43 0.5912874552460536 1101 44-45 0.6029407473862776 1101 46-47 0.928106695677279 1101 48-49 0.951911532879457 1101 50-51 0.9072184992280157 1101 52-53 1.0908576057677735 1101 54-55 1.177482642436189 1101 56-57 1.0937156481619752 1101 58-59 1.1093382621234724 1101 60-61 1.0554861823491208 1101 62-63 1.2325081435541207 1101 64-65 1.1679994472150028 1101 66-67 1.2448165605591583 1101 68-69 1.2389067541281875 1101 70-71 1.1644176539150237 1101 72-73 1.031548410286117 1101 74-75 1.257681973548987 1101 76-77 1.4202902739478667 1101 78-79 1.3225766449372571 1101 80-81 1.427663865202689 1101 82-83 1.260246597057268 1101 84-85 1.5301225421136415 1101 86-87 1.5625143226410394 1101 88-89 1.5958107343465215 1101 90-91 1.7778340042911474 1101 92-93 2.0630545487113174 1101 94-95 1.9515419135315994 1101 96-97 2.2530042687698533 1101 98-99 2.0943563399805427 1101 100-101 2.461387259656018 1102 1 0.2019216528300376 1102 2 0.12001528490737101 1102 3 0.22626114716277712 1102 4 0.18613563921056908 1102 5 0.1719240839791496 1102 6 0.15429554165807247 1102 7 -0.09944845164111626 1102 8 0.25809732013712505 1102 9 0.39869041690185014 1102 10-11 0.13525024650731865 1102 12-13 0.21519457656431484 1102 14-15 0.2591073492658964 1102 16-17 0.22798104560966692 1102 18-19 0.2537268680701317 1102 20-21 0.368283005485317 1102 22-23 0.3792604912295161 1102 24-25 0.5083462900441518 1102 26-27 0.23550208833126618 1102 28-29 0.25926379590077175 1102 30-31 0.3912521010154606 1102 32-33 0.2946849140692436 1102 34-35 0.4974896126644879 1102 36-37 0.5479586158913392 1102 38-39 0.45360847669375914 1102 40-41 0.6331665458594244 1102 42-43 0.6902290476399813 1102 44-45 0.6400793684201247 1102 46-47 0.5727554535171819 1102 48-49 0.5420747470046265 1102 50-51 0.4504110382683777 1102 52-53 0.4209978029496355 1102 54-55 0.39003025641399347 1102 56-57 0.3928528226096688 1102 58-59 0.4845721775838072 1102 60-61 0.7152108690629433 1102 62-63 0.7624574905060868 1102 64-65 0.6610126839290018 1102 66-67 0.7387648958021202 1102 68-69 0.8608720495798892 1102 70-71 0.8465103480916056 1102 72-73 0.887767716803527 1102 74-75 0.7191105675100466 1102 76-77 0.7239500017327991 1102 78-79 0.8310806900833647 1102 80-81 0.7511102215293093 1102 82-83 0.8955207104880998 1102 84-85 0.986168968065666 1102 86-87 1.0397300273391714 1102 88-89 1.1442834818575278 1102 90-91 1.1630650243259133 1102 92-93 1.1643564199223384 1102 94-95 1.2982775746969537 1102 96-97 1.2152158669208362 1102 98-99 1.6619087590128387 1102 100-101 2.437109059957276 1103 1 0.21110278702469643 1103 2 0.1577925780012528 1103 3 0.22071685955364373 1103 4 0.24477055946384496 1103 5 0.11368645186223603 1103 6 0.10908655607665452 1103 7 0.2244272486937149 1103 8 0.2085702590331806 1103 9 0.21623269173707627 1103 10-11 0.203088626945636 1103 12-13 0.31794994725304093 1103 14-15 0.43309751323598533 1103 16-17 0.42285660111179624 1103 18-19 0.30114555423712375 1103 20-21 0.15544647695928404 1103 22-23 0.31509416374615995 1103 24-25 0.37506356228899307 1103 26-27 0.28830133817799464 1103 28-29 0.15150654467669966 1103 30-31 0.17765877866194302 1103 32-33 0.2808739991787874 1103 34-35 0.43608798698891604 1103 36-37 0.3486699562922837 1103 38-39 0.42788607347834784 1103 40-41 0.5256379929213537 1103 42-43 0.6458939242201893 1103 44-45 0.4036731773657536 1103 46-47 0.5274288271403336 1103 48-49 0.6162262305666886 1103 50-51 0.5777539076619007 1103 52-53 0.4036456425093391 1103 54-55 0.39200181310226156 1103 56-57 0.519295782852808 1103 58-59 0.40366192978248705 1103 60-61 0.6699371953555797 1103 62-63 0.618585087548297 1103 64-65 0.7049493742898392 1103 66-67 0.7087636306626592 1103 68-69 0.7850747398044788 1103 70-71 0.8313006240351228 1103 72-73 0.8042894076480955 1103 74-75 0.7116923278628668 1103 76-77 0.7348608277200235 1103 78-79 1.0449639640810808 1103 80-81 0.8694711732576543 1103 82-83 0.8775651758464633 1103 84-85 1.1326080012243445 1103 86-87 1.1531251635935469 1103 88-89 1.0936009076364925 1103 90-91 1.0934860753304996 1103 92-93 1.179073541037404 1103 94-95 1.355918129686728 1103 96-97 1.5467187552742807 1103 98-99 2.002405300487947 1103 100-101 1.9327151333136108 1104 1 0.22209695305290822 1104 2 0.38639153726850495 1104 3 0.3018780753236605 1104 4 0.2981312061454062 1104 5 0.1868492760044802 1104 6 0.2864947221881806 1104 7 0.33294528220509534 1104 8 0.23160004833279402 1104 9 0.38660060688457776 1104 10-11 0.39287564441132616 1104 12-13 0.338837051476645 1104 14-15 0.5362325712225058 1104 16-17 0.5697962563622738 1104 18-19 0.690614338593889 1104 20-21 0.755994192907707 1104 22-23 0.443317473913865 1104 24-25 0.524586031502615 1104 26-27 0.7394841619217729 1104 28-29 0.626876533682541 1104 30-31 0.6408102101675652 1104 32-33 0.529455353290551 1104 34-35 0.6269889108973317 1104 36-37 0.6524349600686961 1104 38-39 0.7757418488568533 1104 40-41 0.8508923348924426 1104 42-43 0.9422039053652895 1104 44-45 0.9248229099376459 1104 46-47 1.1101250603253305 1104 48-49 0.9222525549833378 1104 50-51 0.777913839269857 1104 52-53 0.8453222256601904 1104 54-55 1.0387078196842694 1104 56-57 1.1575080264052815 1104 58-59 0.9920934227250271 1104 60-61 1.0413606953401882 1104 62-63 1.0505824576423564 1104 64-65 0.972174624284861 1104 66-67 1.2632418062469242 1104 68-69 1.1480343620543536 1104 70-71 0.9832619144278141 1104 72-73 1.1576993197853334 1104 74-75 1.2880030697245566 1104 76-77 1.172340790988283 1104 78-79 1.2465774077545468 1104 80-81 1.4431456698715586 1104 82-83 1.4739325729821857 1104 84-85 1.5587442310766768 1104 86-87 1.5404621302542374 1104 88-89 1.4114201505061317 1104 90-91 1.4765648402889937 1104 92-93 1.6010982788165826 1104 94-95 1.7506322360892455 1104 96-97 1.7556251164951213 1104 98-99 1.9073573843986118 1104 100-101 2.3356703361623126 1105 1 0.2853769986610608 1105 2 0.16373534424302605 1105 3 0.2557822588825296 1105 4 0.09150825786233696 1105 5 0.21676916368362242 1105 6 0.2623570251336247 1105 7 0.2979934047687678 1105 8 0.2304408199426078 1105 9 0.1869178093138828 1105 10-11 0.25915852277287854 1105 12-13 0.3242385738200113 1105 14-15 0.343007652378013 1105 16-17 0.49151631538907026 1105 18-19 0.43114757097035294 1105 20-21 0.43819090534106664 1105 22-23 0.5212105826562095 1105 24-25 0.5443956466299085 1105 26-27 0.21423162706089016 1105 28-29 0.17349647608892838 1105 30-31 0.4343886048898966 1105 32-33 0.27030185635889126 1105 34-35 0.3295846819966428 1105 36-37 0.3332099055158366 1105 38-39 0.41796198683547203 1105 40-41 0.4694066327824302 1105 42-43 0.518459001562853 1105 44-45 0.37711983728484455 1105 46-47 0.5888220191264324 1105 48-49 0.6897587744212075 1105 50-51 0.6642758470179189 1105 52-53 0.6374553898019926 1105 54-55 0.6451621471637523 1105 56-57 0.6900304706015561 1105 58-59 0.6076563809389057 1105 60-61 0.6813911092316474 1105 62-63 0.8218815353962654 1105 64-65 0.7425586218598639 1105 66-67 0.7578271062416562 1105 68-69 0.9478813413702483 1105 70-71 1.1799378840925598 1105 72-73 1.166157945541638 1105 74-75 1.248705037051824 1105 76-77 1.130374884224988 1105 78-79 1.0260459383184148 1105 80-81 1.0976309261732808 1105 82-83 1.203904968380531 1105 84-85 1.3454154509489449 1105 86-87 1.3977146955543738 1105 88-89 1.3033788596533213 1105 90-91 1.4092782274110327 1105 92-93 1.6167291546237799 1105 94-95 1.6438265888459966 1105 96-97 1.8218853556589067 1105 98-99 2.0791115290523017 1105 100-101 2.382993235171835 1106 1 0.23475447507810898 1106 2 0.20383326805918145 1106 3 0.34672306249885665 1106 4 0.21943989355479232 1106 5 0.2925005529086846 1106 6 0.24474441550812998 1106 7 0.2736305727147439 1106 8 0.32667086354412334 1106 9 0.23864142321110648 1106 10-11 0.036436757381103746 1106 12-13 0.16988422444419626 1106 14-15 0.14239265820812363 1106 16-17 0.038068811285889126 1106 18-19 0.482155545240488 1106 20-21 0.5724880035467166 1106 22-23 0.5192626060650838 1106 24-25 0.35231548393932854 1106 26-27 0.4315076926455319 1106 28-29 0.32582824551480627 1106 30-31 0.4458523089743025 1106 32-33 0.4463450599929004 1106 34-35 0.4234857883368264 1106 36-37 0.650072129690642 1106 38-39 0.7075473579457423 1106 40-41 0.5993798446504357 1106 42-43 0.5670993553485602 1106 44-45 0.536245347765238 1106 46-47 0.8195038938259458 1106 48-49 0.8586303848037815 1106 50-51 0.6014125010296425 1106 52-53 0.6556057653055589 1106 54-55 0.7702512567312212 1106 56-57 0.7081659945105514 1106 58-59 0.8339231874177884 1106 60-61 0.8831486400262563 1106 62-63 0.9276107279668793 1106 64-65 0.9116471346004573 1106 66-67 1.087521108131611 1106 68-69 1.0612439081678815 1106 70-71 1.0988036229144882 1106 72-73 1.0110295863181697 1106 74-75 1.0944321987532213 1106 76-77 1.2111706672010563 1106 78-79 1.1711132056802747 1106 80-81 1.2568445100633738 1106 82-83 0.8947673003157419 1106 84-85 1.2054906345560958 1106 86-87 1.2878971698438448 1106 88-89 1.3343389081731303 1106 90-91 1.6713415214532645 1106 92-93 1.717218497395816 1106 94-95 1.8528403324425398 1106 96-97 2.0775973480106806 1106 98-99 2.093073064113117 1106 100-101 2.5401213788744776 1107 1 0.19407511608618933 1107 2 0.2551333182806097 1107 3 0.25893828415799547 1107 4 0.182470072069151 1107 5 0.1942149299346667 1107 6 0.22697287736266958 1107 7 0.19325916240565277 1107 8 0.2627241439166781 1107 9 0.16732128441733352 1107 10-11 0.31007507176725824 1107 12-13 0.2178656653426998 1107 14-15 0.4297220194828313 1107 16-17 0.5445148460756428 1107 18-19 0.3753807883578659 1107 20-21 0.46042858246284624 1107 22-23 0.5780388011068567 1107 24-25 0.5983833741826672 1107 26-27 0.4975774091227336 1107 28-29 0.48405999459028237 1107 30-31 0.45408462874784306 1107 32-33 0.44825404506344313 1107 34-35 0.6276045163649542 1107 36-37 0.3881987561801665 1107 38-39 0.4066981592058383 1107 40-41 0.5025837436597058 1107 42-43 0.5538135040953023 1107 44-45 0.5593900083140184 1107 46-47 0.5746059346422712 1107 48-49 0.5992024525570585 1107 50-51 0.6519563762978748 1107 52-53 0.7322990265026519 1107 54-55 0.8030036194212613 1107 56-57 0.8403624972727357 1107 58-59 1.0456750022376795 1107 60-61 1.1054897576813687 1107 62-63 1.0824239955557928 1107 64-65 1.1871830200955955 1107 66-67 1.143917492188585 1107 68-69 1.1424628051226406 1107 70-71 1.2769384674563327 1107 72-73 1.2499009737993383 1107 74-75 1.2517622448656276 1107 76-77 1.2106085295943352 1107 78-79 1.2478407087620447 1107 80-81 1.266044163078945 1107 82-83 1.1437571647178792 1107 84-85 1.440663898492545 1107 86-87 1.4785616906857406 1107 88-89 1.503645053450036 1107 90-91 1.7059800412907293 1107 92-93 1.8601755124165926 1107 94-95 2.2206067343052993 1107 96-97 2.511345008148563 1107 98-99 2.7393743698703226 1107 100-101 3.127258414896943 1108 1 0.21112303297246982 1108 2 0.1038938158385001 1108 3 0.1344185071688706 1108 4 0.16239587175759596 1108 5 0.18662652790601442 1108 6 0.21221334025080552 1108 7 0.08253554831754428 1108 8 0.03068126351563194 1108 9 0.1795450097766249 1108 10-11 0.2445897829892516 1108 12-13 0.304220157770402 1108 14-15 0.47971279568719893 1108 16-17 0.46305189352401754 1108 18-19 0.5835552674330273 1108 20-21 0.5452325987670079 1108 22-23 0.6270376532567354 1108 24-25 0.48509320235653064 1108 26-27 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98-99 -1.2715540844562305 2316 100-101 -1.1110606588260943 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5201.0 3 985.0 4 950.0 5 1040.0 6 1237.0 7 1378.0 8 1694.0 9 2075.0 10 2733.0 11 3447.0 12 4330.0 13 5744.0 14 6407.0 15 7861.0 16 10013.0 17 13132.0 18 11291.0 19 7919.0 20 7679.0 21 8593.0 22 9292.0 23 10124.0 24 11464.0 25 13028.0 26 15446.0 27 17939.0 28 21339.0 29 25712.0 30 31260.0 31 39248.0 32 49396.0 33 65425.0 34 88662.0 35 126626.0 36 185930.0 37 257886.0 38 204227.0 39 23857.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.679564266388596 18.70563300765403 14.728915941420917 22.885886784536456 2 14.931711138733725 19.699516672784082 41.53514223761686 23.83362995086533 3 19.037783200527368 24.369219856515585 30.10404740151349 26.488949541443557 4 12.594087555003252 16.29414521783909 37.52601765654535 33.58574957061231 5 13.709906811207324 37.201283629560486 34.45117744413413 14.637632115098064 6 32.081567551740605 35.68938681789462 17.700913840131914 14.528131790232852 7 28.675869518487502 32.69441904076565 20.72167164510286 17.90803979564399 8 26.272935178935235 35.256759963877 19.922283146399874 18.548021710787896 9 26.32422902600915 17.017215632305778 20.62315934823783 36.03539599344724 10-11 24.877893726991296 26.71435203825104 27.528111316382102 20.879642918375563 12-13 25.67834654042109 23.964430245926145 28.33101400228697 22.026209211365803 14-15 22.50615738637874 25.304556099925147 26.68116007355346 25.508126440142654 16-17 21.79904388063186 28.90059371244993 27.200860191524374 22.099502215393837 18-19 21.72750230673953 27.052632187251387 29.92223542805188 21.2976300779572 20-21 22.455730139714408 26.22301146759835 29.89703429493723 21.42422409775001 22-23 22.131927373462457 26.371608197563656 29.79840427457817 21.69806015439572 24-25 21.997047950012362 26.44372731747865 29.94223781104569 21.6169869214633 26-27 21.819654789576965 26.70942953141649 29.695022778525548 21.775892900480994 28-29 21.593896793204205 26.65876320753554 30.01847822066602 21.72886177859424 30-31 21.738133791327012 26.32685446410925 30.019659671552883 21.915352073010848 32-33 21.53808514737045 26.63363513773839 29.821768445386244 22.006511269504912 34-35 22.084504329233646 26.55204230032072 29.561991351163936 21.801462019281704 36-37 22.015534930481863 26.320363162223114 29.538478131494156 22.12562377580087 38-39 21.8964184309878 26.46680079397822 29.8605838659713 21.776196909062683 40-41 22.005229197224242 26.43151970270304 29.707741184011045 21.855509916061678 42-43 21.948881838367747 26.36150990728342 29.54279647501337 22.14681177933547 44-45 21.948407490455498 26.73762720064777 29.230080003944536 22.083885304952204 46-47 22.01887696612593 26.809228773236033 28.904478021300722 22.26741623933731 48-49 22.250850335877377 26.92670565106893 28.658197145398674 22.16424686765503 50-51 22.123641155155376 27.057728872527203 28.47348964263079 22.34514032968663 52-53 22.343655411297448 27.164093179613868 28.363422535477206 22.12882887361148 54-55 22.088944721875833 26.97892818385746 28.487343388664232 22.444783705602468 56-57 22.118121629391876 27.45975682132325 28.341126435427018 22.080995113857856 58-59 22.237456551671013 27.023214165358805 28.44883929173206 22.290489991238122 60-61 22.284691294211356 26.744102296068995 28.870237610575327 22.100968799144326 62-63 22.218057827141052 26.952731077061507 28.763690823325305 22.065520272472135 64-65 22.201873448085014 26.8880741676228 28.593481177127156 22.31657120716503 66-67 21.941502934790606 27.25428470954644 28.475307046617317 22.32890530904564 68-69 22.025345222733662 27.032991159986903 28.62947056217861 22.312193055100824 70-71 21.970438113080228 27.12797671778012 28.458823964417274 22.442761204722384 72-73 22.090409037283145 27.057117006840976 28.35505747290244 22.49741648297344 74-75 22.025054926092437 27.142387228181324 28.380034635022668 22.45252321070358 76-77 22.067873590782227 27.125629127975177 28.37508258358036 22.431414697662238 78-79 22.032914939625638 27.061883769480616 28.383558941812314 22.521642349081432 80-81 21.903631954730095 27.353470840548 28.382559991679457 22.36033721304245 82-83 21.937295598211087 27.21524351018301 28.404038536358 22.443422355247904 84-85 22.029819287782164 27.08010101025664 28.216851579941544 22.67322812201965 86-87 22.12906312255504 26.885056213879288 28.389395706575414 22.59648495699026 88-89 22.166559578674235 26.816451180972546 28.34861841081419 22.66837082953903 90-91 21.92666855162223 26.95861392591816 28.569827202990457 22.54489031946915 92-93 21.97186516542312 26.850585692989785 28.465719337306005 22.711829804281088 94-95 21.89726222108545 26.7982539600608 28.429580985853104 22.87490283300065 96-97 21.897423098521244 26.752809880240676 28.338867975106822 23.010899046131257 98-99 21.84887414601378 26.823708587181223 28.228796856336956 23.09862041046804 100-101 21.97063300343863 26.363357654337726 28.86630020828968 22.799709133933963 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1783.0 1 1027.0 2 276.0 3 258.0 4 214.0 5 215.0 6 329.0 7 676.0 8 1158.5 9 1402.0 10 1300.5 11 1224.5 12 1282.5 13 1372.5 14 1503.5 15 1633.0 16 1754.5 17 1814.0 18 1864.5 19 1985.0 20 2204.5 21 2511.0 22 2990.0 23 3671.0 24 4490.5 25 5714.0 26 7331.5 27 8907.0 28 10274.0 29 12226.5 30 14732.0 31 17199.0 32 19756.0 33 22565.0 34 25924.5 35 28378.5 36 30432.5 37 33540.5 38 36176.5 39 37881.5 40 40015.5 41 43413.5 42 46765.0 43 49573.5 44 51731.0 45 52643.0 46 52231.5 47 51897.0 48 54359.0 49 54325.5 50 50812.5 51 48276.5 52 45475.0 53 43652.0 54 41442.5 55 37157.0 56 33085.5 57 29145.5 58 25452.0 59 22157.0 60 18444.0 61 14701.5 62 11323.5 63 8350.5 64 6141.5 65 4713.0 66 3599.0 67 2802.5 68 2121.5 69 1462.5 70 1029.0 71 781.0 72 602.0 73 486.0 74 384.0 75 272.5 76 200.5 77 141.0 78 94.5 79 63.5 80 37.0 81 32.5 82 23.5 83 12.0 84 8.0 85 9.0 86 7.5 87 4.5 88 3.5 89 4.0 90 4.5 91 5.0 92 4.0 93 1.0 94 0.5 95 1.0 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30748032979360607 2 0.2865664388949162 3 0.2741872608262063 4 0.25950140361425866 5 0.2546573774134592 6 0.2115224774349113 7 0.20298776460493123 8 0.21398293518769831 9 0.21505938545454267 10-11 0.2174813985549424 12-13 0.21398293518769831 14-15 0.21063825043000345 16-17 0.20917735363928613 18-19 0.2109458076491018 20-21 0.19729795605161118 22-23 0.1905316972314468 24-25 0.19145436888874195 26-27 0.20283398599538205 28-29 0.21736606459778052 30-31 0.21040758251567965 32-33 0.21690472876913294 34-35 0.20825468198199096 36-37 0.19441460712256387 38-39 0.19610617182760492 40-41 0.19030102931712303 42-43 0.20275709669060746 44-45 0.19779773653264607 46-47 0.19891263145187768 48-49 0.2077164568485688 50-51 0.21709695203106943 52-53 0.23162903063346793 54-55 0.20394888091461366 56-57 0.17742207076737831 58-59 0.1794211926915178 60-61 0.18587989429258378 62-63 0.19452994107972574 64-65 0.1827274327968254 66-67 0.1874176803880757 68-69 0.1919157047173895 70-71 0.1855338924210981 72-73 0.1874176803880757 74-75 0.18937835765982786 76-77 0.20325687717164231 78-79 0.2084084605915402 80-81 0.19825907236129364 82-83 0.19806684909935715 84-85 0.19106992236486897 86-87 0.19168503680306573 88-89 0.19783618118503335 90-91 0.19791307048980794 92-93 0.1949912769083733 94-95 0.19591394856566846 96-97 0.18330410258263485 98-99 0.18972435953131356 100-101 0.18291965605876187 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1300571.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.354775611883746 #Duplication Level Percentage of deduplicated Percentage of total 1 77.97405116510575 46.28132310454395 2 12.809305518105262 15.205869095424044 3 4.065609587934573 7.239400344521392 4 1.7815835317596678 4.229819630456898 5 0.9318582170530239 2.765511768763614 6 0.5704032413736184 2.031369384001337 7 0.3753100083442 1.5593508931114946 8 0.24337352955226865 1.1556304989157653 9 0.19690634012232305 1.0518598471065972 >10 0.9377877383703674 10.124879425448114 >50 0.07618928451581922 3.1638175194988882 >100 0.03593535590129972 3.7105243577958453 >500 0.0010378349919228443 0.377610838605256 >1k 6.486468699517776E-4 1.1030332918067483 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4940 0.3798331655865001 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3939 0.3028669715071303 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2577 0.19814373840413174 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1839 0.1413994314804805 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.688930477459516E-5 0.0 10-11 0.0 0.0 0.0 7.688930477459516E-5 0.0 12-13 0.0 0.0 0.0 7.688930477459516E-5 0.0 14-15 0.0 0.0 0.0 7.688930477459516E-5 0.0 16-17 0.0 0.0 0.0 7.688930477459516E-5 0.0 18-19 0.0 0.0 0.0 7.688930477459516E-5 0.0 20-21 0.0 0.0 0.0 7.688930477459516E-5 0.0 22-23 0.0 0.0 0.0 1.5377860954919031E-4 0.0 24-25 7.688930477459516E-5 0.0 0.0 1.5377860954919031E-4 0.0 26-27 7.688930477459516E-5 0.0 0.0 3.844465238729758E-4 0.0 28-29 1.5377860954919031E-4 0.0 0.0 0.0011148949192316298 0.0 30-31 1.5377860954919031E-4 0.0 0.0 0.002729570319498128 0.0 32-33 1.5377860954919031E-4 0.0 0.0 0.0037675759339551628 0.0 34-35 1.5377860954919031E-4 0.0 0.0 0.0053053620294470655 0.0 36-37 1.5377860954919031E-4 0.0 0.0 0.007496707215523028 0.0 38-39 1.5377860954919031E-4 0.0 0.0 0.009149827268176824 0.0 40-41 1.5377860954919031E-4 0.0 0.0 0.012302288763935226 0.0 42-43 2.3066791432378549E-4 0.0 0.0 0.01633897726460147 0.0 44-45 2.3066791432378549E-4 0.0 0.0 0.02633458688529884 0.0 46-47 2.6911256671108307E-4 0.0 0.0 0.049132265750966304 0.0 48-49 3.0755721909838063E-4 0.0 0.0 0.06500990718692021 7.688930477459516E-5 50-51 3.0755721909838063E-4 0.0 0.0 0.08503957108070226 7.688930477459516E-5 52-53 3.0755721909838063E-4 0.0 0.0 0.10787569459875701 7.688930477459516E-5 54-55 3.0755721909838063E-4 0.0 0.0 0.13155760046933232 7.688930477459516E-5 56-57 3.0755721909838063E-4 0.0 0.0 0.16281310286020523 7.688930477459516E-5 58-59 3.0755721909838063E-4 0.0 0.0 0.2123298151350445 7.688930477459516E-5 60-61 3.0755721909838063E-4 0.0 0.0 0.27238036216400335 7.688930477459516E-5 62-63 3.0755721909838063E-4 0.0 0.0 0.34042739688952006 1.5377860954919031E-4 64-65 3.0755721909838063E-4 0.0 0.0 0.4002857206565424 1.5377860954919031E-4 66-67 3.0755721909838063E-4 0.0 0.0 0.49047687515714256 1.5377860954919031E-4 68-69 3.0755721909838063E-4 0.0 0.0 0.5960458906126617 1.5377860954919031E-4 70-71 3.0755721909838063E-4 0.0 0.0 0.6781636681119293 1.5377860954919031E-4 72-73 3.0755721909838063E-4 0.0 0.0 0.8106054955861695 1.5377860954919031E-4 74-75 3.0755721909838063E-4 3.844465238729758E-5 0.0 0.9321290417824171 1.5377860954919031E-4 76-77 3.0755721909838063E-4 7.688930477459516E-5 0.0 1.0524608037546583 2.3066791432378549E-4 78-79 3.0755721909838063E-4 7.688930477459516E-5 0.0 1.2044709592940332 2.3066791432378549E-4 80-81 3.0755721909838063E-4 7.688930477459516E-5 0.0 1.3435252669788884 2.6911256671108307E-4 82-83 3.0755721909838063E-4 7.688930477459516E-5 0.0 1.512181957001963 3.0755721909838063E-4 84-85 3.460018714856782E-4 7.688930477459516E-5 0.0 1.7200137478076938 3.844465238729758E-4 86-87 3.844465238729758E-4 7.688930477459516E-5 0.0 1.990318098742783 3.844465238729758E-4 88-89 3.844465238729758E-4 7.688930477459516E-5 0.0 2.331014608199014 3.844465238729758E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2925 0.0 51.52877 1 GTATCAA 4985 0.0 41.585094 1 ATCAACG 6375 0.0 31.682844 3 TATCAAC 6460 0.0 31.562666 2 TCAACGC 6455 0.0 31.288977 4 CAACGCA 6585 0.0 30.743443 5 AACGCAG 6710 0.0 30.157938 6 GGATCGT 105 6.9042406E-5 27.144169 6 GTACATG 5555 0.0 26.61906 1 ACGCAGA 7650 0.0 26.141787 7 CGCAGAG 7765 0.0 25.5711 8 TACATGG 5725 0.0 25.071505 2 ACATGGG 5700 0.0 24.595892 3 GCAGAGT 8195 0.0 23.589926 9 GTGGTAT 1455 0.0 23.20127 1 GAGTACT 4565 0.0 23.150139 12-13 CATGGGG 2725 0.0 23.020042 4 GTATTCG 105 0.002183981 22.641079 1 GTCTAGG 405 0.0 22.305655 1 TATGACG 110 0.0028696903 21.601107 4 >>END_MODULE