FastQCFastQC Report
Thu 26 May 2016
SRR937845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937845_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300571
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50890.39128967199791476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42960.3303164533116608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28870.22197942288425623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33850.045.7630731
GTATCAA52200.035.8659171
ATCAACG66550.027.976533
TATCAAC67600.027.6122422
TCAACGC67850.027.4405024
CTATACG700.0083955327.1404974
CAACGCA69250.026.9543345
AACGCAG70450.026.4952136
GTACATG56300.025.4047131
TGTACGC1151.2826374E-424.7804532
TACATGG57550.024.5938642
ACCGTCG1201.710656E-423.7506738
ACATGGG58050.023.4820213
GTATAGG3450.023.4145621
ACGCAGA81200.022.9901837
CCTATAC3550.022.7445013
GAGTACT46850.022.55933612-13
CGCAGAG82700.022.4008778
GTACTTT50300.021.05681614-15
GCGCGCC3451.8189894E-1220.6527589
Produced by FastQC (version 0.11.5)