FastQCFastQC Report
Thu 26 May 2016
SRR937844_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937844_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094903
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45050.41145197337115713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34360.3138177537188226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22210.2028490195021842No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15310.13982973834211798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25750.046.1761171
GTATCAA43450.039.1875271
ATCAACG53650.031.3624323
TCAACGC54450.030.9044724
TATCAAC54850.030.6762922
CAACGCA55250.030.370955
AACGCAG57000.029.5043496
GTACATG42000.026.9514581
ATACCGA908.939571E-426.3927786
ACGCAGA65300.025.8281127
CGCAGAG66450.025.3096288
TACATGG44150.024.7612932
ACATGGG45050.023.6335773
GCAGAGT69450.023.599599
GAGTACT38050.023.4672112-13
GCACCGT2955.456968E-1222.5456946
TGCACCG2755.0931703E-1122.4712185
GTACTTT42750.020.71816814-15
AGTACTT41000.020.44650712-13
CATGGGG21950.020.356794
Produced by FastQC (version 0.11.5)