FastQCFastQC Report
Thu 26 May 2016
SRR937844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937844_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094903
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43560.39784346193224424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36490.3332715318160604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24680.22540809551165722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27950.043.359661
GTATCAA46500.036.9984131
TATCAAC58400.029.121862
ATCAACG59100.028.7769323
TCAACGC59600.028.4558054
CAACGCA61500.027.653935
AACGCAG62100.027.5347086
GTACATG46900.025.5361371
ACGCAGA70800.024.018137
CGCAGAG71400.023.616728
TACATGG49750.023.4904292
ACATGGG49200.023.2702393
GAGTACT40400.021.92738712-13
GCAGAGT77250.021.6438069
ACGTATA1100.00287515421.5936582
GTACCGT1100.002878203521.5897146
CGATCGT1259.506039E-920.89884424-25
AGTACTT43600.020.53592712-13
AGGACCG1404.84737E-420.3597355
GTACTTT45250.019.89116514-15
Produced by FastQC (version 0.11.5)