FastQCFastQC Report
Thu 26 May 2016
SRR937843_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937843_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012384
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38280.3781173941903468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31590.3120357492809053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20160.19913392546701647No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13150.12989142459778108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26750.042.673911
GTATCAA43250.035.191591
ATCAACG52800.028.3690013
TATCAAC53150.028.1821862
TCAACGC53200.028.1556974
CAACGCA53450.028.0240085
AACGCAG54900.027.5205156
GTACGGG700.00833647927.1792161
GTACATG33550.024.5261041
ACGCAGA61900.024.3339927
TACATGG32700.024.284892
CGCAGAG61550.024.2383678
ACATGGG33600.022.9267833
GAGTACT38500.022.64244712-13
ATACCGT1050.00219286322.624686
GTACTAG1503.001555E-522.1963631
GTACCTG7750.022.0940741
GCAGAGT66650.021.5293069
GTACTTT41750.020.87831114-15
GTGTAGC7750.020.8666251
Produced by FastQC (version 0.11.5)