##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937843_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1012384 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.486878496696907 33.0 31.0 34.0 30.0 34.0 2 31.61753544109745 33.0 31.0 34.0 30.0 34.0 3 31.6357311059835 33.0 31.0 34.0 30.0 34.0 4 35.22184665107311 37.0 35.0 37.0 33.0 37.0 5 35.20748154850333 37.0 35.0 37.0 33.0 37.0 6 35.26102842399722 37.0 35.0 37.0 33.0 37.0 7 35.191862968992005 37.0 35.0 37.0 32.0 37.0 8 35.17432910832253 37.0 35.0 37.0 32.0 37.0 9 36.77724460283845 39.0 37.0 39.0 33.0 39.0 10-11 36.644745472073836 39.0 37.0 39.0 32.0 39.0 12-13 36.51245426636533 39.0 37.0 39.0 32.0 39.0 14-15 37.62350303837279 40.0 37.5 41.0 32.0 41.0 16-17 37.51600874766886 40.0 37.0 41.0 32.0 41.0 18-19 37.43275624664159 40.0 37.0 41.0 32.0 41.0 20-21 37.324688063027466 40.0 37.0 41.0 32.0 41.0 22-23 37.228441480702976 40.0 37.0 41.0 31.5 41.0 24-25 37.25490327780763 40.0 37.0 41.0 31.5 41.0 26-27 37.16162394901539 40.0 37.0 41.0 31.0 41.0 28-29 37.02494359847647 40.0 37.0 41.0 31.0 41.0 30-31 36.76954594304138 39.0 36.0 41.0 30.0 41.0 32-33 36.604161563201316 39.0 36.0 41.0 30.0 41.0 34-35 36.58051984227329 39.0 36.0 41.0 30.0 41.0 36-37 36.42534502718336 39.0 36.0 41.0 30.0 41.0 38-39 36.24922213310364 39.0 35.0 41.0 30.0 41.0 40-41 36.00260523674811 39.0 35.0 41.0 28.5 41.0 42-43 35.7194774907545 39.0 35.0 40.5 27.0 41.0 44-45 35.48581516499668 39.0 35.0 40.0 26.5 41.0 46-47 35.13882183045169 38.5 35.0 40.0 25.5 41.0 48-49 34.80610173610013 38.0 34.0 40.0 24.0 41.0 50-51 33.91995280446945 37.5 33.5 39.5 23.0 40.5 52-53 34.01712887599962 38.0 33.5 39.5 23.0 40.5 54-55 34.281317662072894 38.0 34.0 40.0 23.0 41.0 56-57 34.02193387094225 38.0 33.5 40.0 21.5 41.0 58-59 33.73834829471821 38.0 33.0 40.0 20.0 41.0 60-61 33.39337296915004 37.5 33.0 40.0 18.5 41.0 62-63 32.989101467427375 37.0 32.0 40.0 16.5 41.0 64-65 32.570629326421596 36.5 32.0 39.0 13.0 41.0 66-67 32.11577375778361 36.0 31.0 39.0 8.0 40.5 68-69 31.58610912460094 35.0 31.0 39.0 7.5 40.0 70-71 31.10838822028005 35.0 30.0 38.0 4.5 40.0 72-73 30.62513384249455 35.0 30.0 37.5 2.0 39.5 74-75 30.053121147706797 34.5 29.0 37.0 2.0 39.0 76-77 29.547718059550526 34.0 29.0 36.0 2.0 39.0 78-79 28.95611003334703 34.0 28.5 36.0 2.0 38.5 80-81 28.36197233460821 34.0 27.0 35.0 2.0 37.0 82-83 27.828226740051207 33.5 26.0 35.0 2.0 37.0 84-85 27.28104750766508 33.0 26.0 35.0 2.0 36.0 86-87 26.713155284951164 32.5 25.0 35.0 2.0 36.0 88-89 26.180612297310113 32.0 24.0 35.0 2.0 35.5 90-91 25.56731684815248 32.0 21.5 34.5 2.0 35.0 92-93 24.896516539178812 31.5 18.5 34.0 2.0 35.0 94-95 24.111620689382683 31.0 10.0 34.0 2.0 35.0 96-97 23.230942014097415 31.0 2.0 34.0 2.0 35.0 98-99 22.126425842368114 30.5 2.0 34.0 2.0 35.0 100-101 19.347420050099565 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08225917655763126 1101 2 0.2130374637588197 1101 3 0.2950323059501052 1101 4 0.1239186199399569 1101 5 0.01703386776531346 1101 6 0.1712386441573699 1101 7 0.2294883856074179 1101 8 0.23757840640951855 1101 9 0.3831407801392501 1101 10-11 0.473552215103183 1101 12-13 0.319736441599197 1101 14-15 0.3775771470580267 1101 16-17 0.4023539959604676 1101 18-19 0.38005520802387593 1101 20-21 0.34954973721279003 1101 22-23 0.37482527844956337 1101 24-25 0.1849428232492798 1101 26-27 0.25152038113012765 1101 28-29 0.285532351484413 1101 30-31 0.40588572398709744 1101 32-33 0.18349562746849557 1101 34-35 0.34489977749326783 1101 36-37 0.48704619555579143 1101 38-39 0.25274320198715117 1101 40-41 0.360611705460677 1101 42-43 0.49951185453136304 1101 44-45 0.6165466924882779 1101 46-47 0.8424918376475503 1101 48-49 0.8442683575827132 1101 50-51 0.678214386884207 1101 52-53 0.6400026277765178 1101 54-55 0.6188295976381468 1101 56-57 0.49363997211440136 1101 58-59 0.47646701074999953 1101 60-61 0.5638697512242246 1101 62-63 0.6491595705342306 1101 64-65 0.5772573520933904 1101 66-67 0.6227328569036601 1101 68-69 0.7211254647594387 1101 70-71 0.6747430806266728 1101 72-73 0.599016873552177 1101 74-75 0.6941939253911187 1101 76-77 0.9122835489318355 1101 78-79 1.0869014610201972 1101 80-81 0.9655246730298046 1101 82-83 1.0091688431695687 1101 84-85 0.9549002731505283 1101 86-87 1.2299985516708105 1101 88-89 1.2380869078525016 1101 90-91 0.9877759046997063 1101 92-93 1.0743971489793545 1101 94-95 1.0616558997554613 1101 96-97 0.9043464218787456 1101 98-99 0.9407555255733548 1101 100-101 0.7988074179002744 1102 1 0.18419144059150838 1102 2 0.29712947874372375 1102 3 0.2746650274540947 1102 4 0.3039066088622704 1102 5 0.31278042679053186 1102 6 0.1295716006371066 1102 7 0.21923917383438862 1102 8 0.1453056536477888 1102 9 0.40593469428537077 1102 10-11 0.29733668137901503 1102 12-13 0.3800301522443732 1102 14-15 0.49485880863868204 1102 16-17 0.625009800802161 1102 18-19 0.559378879434071 1102 20-21 0.7283472908601638 1102 22-23 0.5812783384705824 1102 24-25 0.7523979580754343 1102 26-27 0.7258760971549592 1102 28-29 0.7483803154801194 1102 30-31 0.6693647217856622 1102 32-33 0.6305464918500334 1102 34-35 0.8097565218761957 1102 36-37 0.7059838121165996 1102 38-39 0.804150704931132 1102 40-41 0.899503900611748 1102 42-43 0.8655553259498774 1102 44-45 0.7761778040737184 1102 46-47 0.8533212532109431 1102 48-49 0.8405958439571961 1102 50-51 1.0108470740191464 1102 52-53 1.0491607127331903 1102 54-55 0.6569199990867176 1102 56-57 0.6499793429437588 1102 58-59 0.7319538483092174 1102 60-61 0.8029078339378586 1102 62-63 0.8928564680945712 1102 64-65 0.7975579727260325 1102 66-67 0.8511323627129315 1102 68-69 0.9183468177060377 1102 70-71 0.8375329790209136 1102 72-73 0.9654623723827278 1102 74-75 0.9201401143159629 1102 76-77 0.9447175287047465 1102 78-79 0.9001699031798047 1102 80-81 0.925481114784219 1102 82-83 1.1326346721962182 1102 84-85 1.2341401926184226 1102 86-87 1.3720223097271855 1102 88-89 1.07541051776019 1102 90-91 1.2646769200337324 1102 92-93 1.2684701162763012 1102 94-95 1.5936706675925443 1102 96-97 1.5909472868934067 1102 98-99 1.8419635071515437 1102 100-101 1.936458619098488 1103 1 0.2703232760626051 1103 2 0.3495746360934646 1103 3 0.213396715650628 1103 4 0.09776788535599934 1103 5 0.27439580423393295 1103 6 0.11418197949807052 1103 7 0.2458408798598981 1103 8 0.36027419131031024 1103 9 0.3776242988702947 1103 10-11 0.5471640048065183 1103 12-13 0.18269079853996573 1103 14-15 0.3923436292738671 1103 16-17 0.4013417888755839 1103 18-19 0.4107433573419357 1103 20-21 0.4732321636313017 1103 22-23 0.21606310639897686 1103 24-25 0.39203717344830835 1103 26-27 0.37560333476744034 1103 28-29 0.5154586469011662 1103 30-31 0.3367744209764183 1103 32-33 0.34182507862310985 1103 34-35 0.3910086981718379 1103 36-37 0.4072276542702369 1103 38-39 0.422139703102566 1103 40-41 0.6392527156603904 1103 42-43 0.3784418503272491 1103 44-45 0.4106700207842451 1103 46-47 0.3220772588355416 1103 48-49 0.39639844276339886 1103 50-51 0.3044514762727957 1103 52-53 0.37139089488556465 1103 54-55 0.5523136731304277 1103 56-57 0.4641988543499309 1103 58-59 0.2049692093887714 1103 60-61 0.28982770249435674 1103 62-63 0.21715924480997018 1103 64-65 0.2161711753008646 1103 66-67 0.29473609899632436 1103 68-69 0.15915547684153353 1103 70-71 0.37252020190593527 1103 72-73 -0.04831464936025043 1103 74-75 -0.07069375655050081 1103 76-77 0.22170644886252333 1103 78-79 0.5052980643978842 1103 80-81 0.5663637917563022 1103 82-83 0.42809380160184674 1103 84-85 0.2718621747742276 1103 86-87 0.46664764437723605 1103 88-89 0.7652981400370891 1103 90-91 0.6576155423125272 1103 92-93 0.7027987897206422 1103 94-95 0.3925623979545101 1103 96-97 0.48223144878887325 1103 98-99 0.5102351621256744 1103 100-101 0.7659925921438209 1104 1 0.22285602653738934 1104 2 0.27245385238282793 1104 3 0.18305194513100176 1104 4 0.19823227508008756 1104 5 0.22744420692298917 1104 6 0.30752431404031455 1104 7 0.40767999233262486 1104 8 0.43745350306092234 1104 9 0.3920797156403921 1104 10-11 0.33851557870623594 1104 12-13 0.5282554831242834 1104 14-15 0.5548976920280282 1104 16-17 0.523038738757414 1104 18-19 0.31831295020878514 1104 20-21 0.6146278353818317 1104 22-23 0.5117316277990511 1104 24-25 0.2540891059836454 1104 26-27 0.43887012777394574 1104 28-29 0.5422135294515442 1104 30-31 0.6263741900933937 1104 32-33 0.6359047655586423 1104 34-35 0.5478840273920653 1104 36-37 0.6429130075273264 1104 38-39 0.7735158184149142 1104 40-41 0.777571124343531 1104 42-43 0.7045015610372758 1104 44-45 0.7478808367660363 1104 46-47 0.6146664989599415 1104 48-49 0.6884725352255927 1104 50-51 0.8229180819895703 1104 52-53 0.8004564240900507 1104 54-55 0.9124987630113353 1104 56-57 0.8369160085148053 1104 58-59 0.9027299063971839 1104 60-61 0.9625172072058703 1104 62-63 1.02407826823935 1104 64-65 1.273151754082626 1104 66-67 1.211897731242452 1104 68-69 1.0290198936096147 1104 70-71 1.0923020739555653 1104 72-73 1.101764363493718 1104 74-75 1.149429697249687 1104 76-77 1.0339237735659985 1104 78-79 0.8488243586243911 1104 80-81 0.9042769442178091 1104 82-83 1.0857143542276404 1104 84-85 1.3067655732351433 1104 86-87 1.5209773065897174 1104 88-89 1.358999009279394 1104 90-91 1.5819105317068427 1104 92-93 1.091154535256873 1104 94-95 0.9737745943907576 1104 96-97 1.1466881773943527 1104 98-99 1.0618609298016466 1104 100-101 1.0350555784058741 1105 1 0.48345428957737013 1105 2 0.31377317400216853 1105 3 0.17510487827275512 1105 4 0.3682176978053988 1105 5 0.2761137306102768 1105 6 0.3889389670709136 1105 7 0.36800830970608445 1105 8 0.46616457772014996 1105 9 0.30417676669006966 1105 10-11 0.45089833181812367 1105 12-13 0.3436969595924708 1105 14-15 0.4590878498111195 1105 16-17 0.38803094925452086 1105 18-19 0.4165660772272517 1105 20-21 0.24593443710293883 1105 22-23 0.5571549165866543 1105 24-25 0.6090165274338446 1105 26-27 0.47462201567189055 1105 28-29 0.36496242572123805 1105 30-31 0.3874347103048805 1105 32-33 0.23329755163373989 1105 34-35 0.34107142102335075 1105 36-37 0.42029131024003163 1105 38-39 0.31492070456713606 1105 40-41 0.3632582649333642 1105 42-43 0.3231993940633018 1105 44-45 0.19105816091265382 1105 46-47 0.26418530280950847 1105 48-49 0.21992167493682047 1105 50-51 0.20745970130878533 1105 52-53 0.2896580756942271 1105 54-55 0.32034263069517266 1105 56-57 0.3925047811959317 1105 58-59 0.3857516074409659 1105 60-61 0.3690979545598836 1105 62-63 0.31618410530570173 1105 64-65 0.2904884849540039 1105 66-67 0.44734419549681803 1105 68-69 0.40435393441554623 1105 70-71 0.3878826658325707 1105 72-73 0.3599276894914567 1105 74-75 0.5092593403516688 1105 76-77 0.4446830961870667 1105 78-79 0.6658654744693813 1105 80-81 0.545920158898177 1105 82-83 0.5491751682802573 1105 84-85 0.6145344154322281 1105 86-87 0.5692242249621984 1105 88-89 0.8598536111382273 1105 90-91 1.0109707513989967 1105 92-93 0.8947307153430835 1105 94-95 0.9201898056777615 1105 96-97 1.0582713195118245 1105 98-99 1.2598906499117142 1105 100-101 1.7495131670929887 1106 1 0.12906913512981077 1106 2 0.37576832202003985 1106 3 0.17175225587907406 1106 4 0.2846898529391524 1106 5 0.19348955390735512 1106 6 0.18680636423644614 1106 7 0.34521804176645077 1106 8 0.3304333494338536 1106 9 0.24417130956095434 1106 10-11 0.177340823943112 1106 12-13 0.10558715242904526 1106 14-15 0.20238192963649482 1106 16-17 0.0984593338902684 1106 18-19 0.13124737548366738 1106 20-21 0.13855596628776823 1106 22-23 0.43636595619676655 1106 24-25 0.48154663619820326 1106 26-27 0.3892827821553837 1106 28-29 0.580160395236959 1106 30-31 0.6553480067890902 1106 32-33 0.4491614529947796 1106 34-35 0.6574836017677939 1106 36-37 0.4989604191070498 1106 38-39 0.5521349482144373 1106 40-41 0.8073118852792689 1106 42-43 0.7787224409784059 1106 44-45 0.6561750839563345 1106 46-47 0.8488835938095676 1106 48-49 0.9713062188342931 1106 50-51 1.0192326469158033 1106 52-53 0.8795264562236227 1106 54-55 0.969609970587868 1106 56-57 0.973352013999218 1106 58-59 0.6974489096740655 1106 60-61 0.9350150764375726 1106 62-63 0.9855556979769347 1106 64-65 1.154522221898766 1106 66-67 1.231087218746353 1106 68-69 1.0121484437872255 1106 70-71 0.7892234446351374 1106 72-73 1.113122946651913 1106 74-75 1.095395530843298 1106 76-77 1.1699382574965647 1106 78-79 1.2215592592855948 1106 80-81 0.9925794233203966 1106 82-83 1.0725300599751542 1106 84-85 1.1172238075296583 1106 86-87 1.5251680381446775 1106 88-89 1.4402375282763167 1106 90-91 1.6922413169412813 1106 92-93 1.7082812802369922 1106 94-95 1.6746696494201636 1106 96-97 2.1172650465148877 1106 98-99 2.089048739160088 1106 100-101 1.9362084431642934 1107 1 0.2950663491047969 1107 2 0.2769588755064092 1107 3 0.2869148428685726 1107 4 0.2404543275786537 1107 5 0.2891352093020316 1107 6 0.3319246227347108 1107 7 0.2487696334012952 1107 8 0.32515052657664256 1107 9 0.3599403882248424 1107 10-11 0.18799058164157145 1107 12-13 0.38668866526423784 1107 14-15 0.42712693751645503 1107 16-17 0.5692112360016282 1107 18-19 0.39954984808086635 1107 20-21 0.4393787880825144 1107 22-23 0.4159901969754429 1107 24-25 0.5333064950081265 1107 26-27 0.6355243045527885 1107 28-29 0.6232087611034274 1107 30-31 0.5611374198940311 1107 32-33 0.5788082712007494 1107 34-35 0.6716188420329345 1107 36-37 0.7269894834866619 1107 38-39 0.7516655443175466 1107 40-41 0.5703639992938037 1107 42-43 0.5076501755032794 1107 44-45 0.5823092676569033 1107 46-47 0.8338400916816866 1107 48-49 0.6792487511657583 1107 50-51 0.6206252167287474 1107 52-53 0.7709024692565478 1107 54-55 0.8988275225454672 1107 56-57 0.8803367026668241 1107 58-59 0.9652946689544208 1107 60-61 1.0652019821199943 1107 62-63 0.9623621872902888 1107 64-65 0.7191792515947171 1107 66-67 0.825598838005341 1107 68-69 1.1024847146455201 1107 70-71 1.088276963467365 1107 72-73 1.18594400357064 1107 74-75 1.217290027853636 1107 76-77 1.1598330142210962 1107 78-79 0.9515701200610778 1107 80-81 1.1255132493236921 1107 82-83 1.2088564026680366 1107 84-85 1.2010303322585791 1107 86-87 1.331159320011313 1107 88-89 1.5761617566595376 1107 90-91 1.5841345448936117 1107 92-93 1.677054337250162 1107 94-95 1.58080879130085 1107 96-97 1.8318096317690227 1107 98-99 1.8788204267711315 1107 100-101 1.9151885632230794 1108 1 0.3846983445413983 1108 2 0.16050885357735112 1108 3 0.39076036543935544 1108 4 0.4247297134897039 1108 5 0.4042506530089369 1108 6 0.2738435632518019 1108 7 0.4349042805049095 1108 8 0.3691942985897825 1108 9 0.3859417927607893 1108 10-11 0.5410652238481433 1108 12-13 0.6031708408419973 1108 14-15 0.7585193923062761 1108 16-17 0.7516959731085535 1108 18-19 0.8109322397203798 1108 20-21 0.7082525762666521 1108 22-23 0.7776797299537819 1108 24-25 0.7107791644067234 1108 26-27 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#Quality Count 2 5761.0 3 1454.0 4 1347.0 5 1325.0 6 1511.0 7 1847.0 8 2075.0 9 2668.0 10 3199.0 11 3876.0 12 4946.0 13 6012.0 14 6757.0 15 7858.0 16 9354.0 17 11381.0 18 10120.0 19 8240.0 20 8353.0 21 8669.0 22 9700.0 23 10546.0 24 11852.0 25 13350.0 26 15177.0 27 17377.0 28 20326.0 29 24330.0 30 28785.0 31 35443.0 32 43588.0 33 55679.0 34 73324.0 35 100207.0 36 138820.0 37 176603.0 38 119365.0 39 11159.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80375758056205 18.63599429228269 14.789032462642199 22.77121566451306 2 15.261431240206111 19.70287987416275 41.40980508301653 23.62588380261461 3 19.348007183391427 24.116265547753407 29.800277549128275 26.73544971972689 4 12.609268872994035 16.52922933066284 37.48062257379765 33.38087922254547 5 13.604338996483234 36.987567487245535 34.71593441973352 14.692159096537718 6 31.89691346051074 35.96321250291948 17.73948688309786 14.400387153471911 7 28.275475757614743 32.607841526620554 20.795573417943256 18.321109297821447 8 25.8191833749494 35.419481070874 19.79242439087633 18.968911163300266 9 25.719909860961426 17.32929944311334 20.701002237641262 36.24978845828397 10-11 24.66260552855828 26.700580963786262 27.534912261601956 21.101901246053504 12-13 25.549202736303023 24.08481054936355 28.129640396343348 22.23634631799008 14-15 22.497384836306093 25.611235752677263 26.45031485878116 25.44106455223548 16-17 21.808998377400187 28.816561107781897 27.19163552023895 22.182804994578966 18-19 21.668032348854606 27.251594285510706 29.774098352868528 21.30627501276616 20-21 22.220188125371397 26.337947819478412 30.104116621699383 21.33774743345081 22-23 22.046346488389627 26.19854100985392 29.98074338833243 21.774369113424015 24-25 21.82285450770598 26.448421298467906 30.028678474518912 21.700045719307205 26-27 21.559531348955037 26.684016782773906 29.922518207726412 21.83393366054465 28-29 21.60584822928558 26.839534433760825 29.952183510808073 21.60243382614552 30-31 21.689209385002446 26.621125954840664 29.90207625265 21.787588407506895 32-33 21.543472010317263 26.598425983982704 29.953862317395384 21.904239688304646 34-35 22.024109097153456 26.69860560361572 29.703112388536624 21.5741729106942 36-37 22.033543599257886 26.426221397649968 29.69682127396413 21.843413729128013 38-39 21.634478862313898 26.788666673922485 29.74107608419257 21.835778379571043 40-41 22.083271696953837 26.71097229801367 29.589766398533985 21.615989606498513 42-43 21.761128895793856 26.685352953109458 29.497746265628944 22.055771885467742 44-45 21.865445619167172 26.84556186872123 29.484902748494925 21.80408976361667 46-47 22.068826283552887 27.06933595392976 28.912066520396216 21.949771242121138 48-49 22.080317795540847 27.089665014285558 28.77933933125573 22.05067785891786 50-51 22.08870318063866 27.422047730363232 28.632180882122988 21.85706820687512 52-53 22.229459706406722 27.35056272539456 28.51165097463504 21.908326593563675 54-55 22.01658245809857 27.30183279800037 28.710174097009062 21.971410646892 56-57 22.006011871368116 27.60676859935073 28.50746091442582 21.879758614855337 58-59 22.155315070187374 27.250015459773667 28.54269989487354 22.05196957516542 60-61 22.043734163877467 27.318566638601503 28.78184662174262 21.85585257577841 62-63 21.933873004863575 27.391653016568608 28.924339291553725 21.750134687014093 64-65 21.896193573058632 27.34727517652396 28.71885136863268 22.037679881784726 66-67 21.77995355799759 27.489232955023287 28.569251911750303 22.161561575228824 68-69 21.868163184126864 27.365519739009887 28.640884294977404 22.125432781885845 70-71 21.91252562001297 27.351859061853624 28.563018429589054 22.172596888544348 72-73 21.911473341825694 27.295843278875047 28.60343569511607 22.18924768418319 74-75 21.880630508065384 27.65725350433422 28.285001043854763 22.177114943745625 76-77 21.99439764269586 27.445515046811643 28.28042657864376 22.27966073184874 78-79 22.017102237420694 27.444420696834047 28.344554079245775 22.193922986499484 80-81 22.09307617797188 27.634587547825813 28.1802495104873 22.092086763715013 82-83 22.017996429209248 27.650327668780218 28.2362084514651 22.09546745054544 84-85 22.02355776579657 27.615892958641087 28.21099589957749 22.14955337598485 86-87 22.185897930554155 27.51404330422773 28.17821887966189 22.12183988555622 88-89 22.09183602801066 27.48343322536156 28.07635457745573 22.348376169172045 90-91 22.067740682749697 27.412838227255076 28.164055833005584 22.355365256989636 92-93 22.11753974523673 27.215723675195875 28.32996053053998 22.33677604902741 94-95 22.0729790082208 27.244790641210454 28.30358088073064 22.37864946983811 96-97 22.084897773424554 27.1385957882552 28.264003284705836 22.512503153614414 98-99 21.934611914071773 27.13653057670693 28.143501955072896 22.785355554148396 100-101 22.298353690656075 26.426071881669504 28.732586637121965 22.542987790552456 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1272.0 1 743.5 2 259.0 3 291.0 4 237.0 5 191.0 6 306.5 7 653.0 8 1072.5 9 1299.0 10 1155.5 11 957.5 12 977.5 13 1066.0 14 1167.0 15 1270.0 16 1351.5 17 1429.5 18 1555.0 19 1714.0 20 1904.5 21 2244.0 22 2691.5 23 3324.5 24 4153.0 25 5164.5 26 6508.0 27 8095.0 28 9545.5 29 11044.5 30 12860.0 31 14895.5 32 17316.5 33 19179.5 34 20885.0 35 22712.5 36 24889.0 37 27174.5 38 28599.0 39 29715.5 40 31024.0 41 32921.5 42 34180.5 43 35503.0 44 36826.0 45 37739.5 46 38460.5 47 38865.5 48 39768.0 49 39382.0 50 37697.5 51 36039.5 52 34458.5 53 33538.5 54 32683.5 55 29881.0 56 26383.0 57 23434.5 58 20836.0 59 18096.5 60 14652.5 61 11851.0 62 9568.0 63 7199.0 64 5266.5 65 3989.0 66 2910.0 67 2094.0 68 1577.0 69 1167.0 70 846.5 71 618.0 72 465.5 73 335.0 74 257.5 75 184.0 76 129.0 77 89.0 78 65.5 79 46.5 80 22.5 81 16.5 82 15.0 83 13.5 84 11.0 85 11.0 86 9.5 87 6.5 88 3.5 89 2.0 90 2.5 91 1.0 92 1.0 93 1.0 94 0.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31845623794923666 2 0.27914309194929987 3 0.2798345291905048 4 0.2798345291905048 5 0.2898109808136043 6 0.19083667857255746 7 0.1919232228087366 8 0.19330609729114645 9 0.1921207763062237 10-11 0.19696083699465816 12-13 0.18733010399216107 14-15 0.1907379018238139 16-17 0.19444202990169737 18-19 0.18767582261276353 20-21 0.18120594557006037 22-23 0.17997123621076588 24-25 0.18471252015045675 26-27 0.180168789708253 28-29 0.19345426241426178 30-31 0.1981461579795809 32-33 0.20135640231374657 34-35 0.19938086733887536 36-37 0.17374830103992162 38-39 0.16801924961279516 40-41 0.17310625217308848 42-43 0.18165044093940638 44-45 0.18624355975598192 46-47 0.19034279482883965 48-49 0.18994768783386543 50-51 0.19340487403989 52-53 0.21839539147201062 54-55 0.17760059424092045 56-57 0.1695996775926921 58-59 0.16757475424344911 60-61 0.16209264468818155 62-63 0.16866129847962827 64-65 0.16648821000726996 66-67 0.16406817966305276 68-69 0.1749336220248443 70-71 0.1678710844896798 72-73 0.16510533552486015 74-75 0.1686119101052565 76-77 0.17167398931630687 78-79 0.17345197079369093 80-81 0.1664388216328982 82-83 0.16629065650978286 84-85 0.16145059582134844 86-87 0.15626481651231153 88-89 0.16061099345702817 90-91 0.16708087049973133 92-93 0.1582897398615545 94-95 0.16347551917059142 96-97 0.16189509119069442 98-99 0.16594493788918038 100-101 0.14683163700730156 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1012384.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.48038822658874 #Duplication Level Percentage of deduplicated Percentage of total 1 85.91693890143655 61.41376147914789 2 8.88530185643659 12.702496524170343 3 2.231028990076282 4.784244550662805 4 0.9351314595089308 2.673742390743797 5 0.5124077778612479 1.8313553445922819 6 0.324334146235838 1.3910118412846144 7 0.2385334179216163 1.193532292263659 8 0.16104748201727284 0.9209389230007384 9 0.11783821018888244 0.7580808911004914 >10 0.6319774865247962 7.884087791051305 >50 0.030222723582541487 1.4191095398148543 >100 0.01440640975880953 1.8699336338064951 >500 2.7704615024702346E-4 0.1360491270054494 >1k 5.540923004940469E-4 1.0216556713553326 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3828 0.3781173941903468 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3159 0.3120357492809053 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2016 0.19913392546701647 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1315 0.12989142459778108 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.877674874355975E-5 0.0 5 0.0 0.0 0.0 9.877674874355975E-5 0.0 6 0.0 0.0 0.0 9.877674874355975E-5 0.0 7 0.0 0.0 0.0 9.877674874355975E-5 0.0 8 0.0 0.0 0.0 9.877674874355975E-5 0.0 9 0.0 0.0 0.0 1.975534974871195E-4 0.0 10-11 0.0 0.0 0.0 1.975534974871195E-4 0.0 12-13 0.0 0.0 0.0 1.975534974871195E-4 9.877674874355975E-5 14-15 0.0 0.0 0.0 1.975534974871195E-4 1.4816512311533963E-4 16-17 0.0 0.0 0.0 1.975534974871195E-4 1.975534974871195E-4 18-19 0.0 0.0 0.0 1.975534974871195E-4 1.975534974871195E-4 20-21 0.0 0.0 0.0 2.9633024623067927E-4 1.975534974871195E-4 22-23 0.0 0.0 0.0 4.444953693460189E-4 1.975534974871195E-4 24-25 0.0 0.0 0.0 6.914372412049183E-4 1.975534974871195E-4 26-27 0.0 0.0 0.0 7.90213989948478E-4 1.975534974871195E-4 28-29 0.0 0.0 0.0 0.001580427979896956 1.975534974871195E-4 30-31 0.0 0.0 0.0 0.003062079211050352 1.975534974871195E-4 32-33 0.0 0.0 0.0 0.006222935170844265 1.975534974871195E-4 34-35 0.0 0.0 0.0 0.010025839997471316 1.975534974871195E-4 36-37 0.0 0.0 0.0 0.014075686695957264 1.975534974871195E-4 38-39 0.0 0.0 0.0 0.017878591522584315 1.975534974871195E-4 40-41 0.0 0.0 0.0 0.02059495211303221 1.975534974871195E-4 42-43 0.0 0.0 0.0 0.0238051964471979 1.975534974871195E-4 44-45 0.0 0.0 0.0 0.026027673293927994 1.975534974871195E-4 46-47 0.0 0.0 0.0 0.031756724721054466 1.975534974871195E-4 48-49 0.0 0.0 0.0 0.037189445901950245 1.975534974871195E-4 50-51 0.0 0.0 0.0 0.04207889496475646 1.975534974871195E-4 52-53 0.0 0.0 0.0 0.05017858836172835 2.469418718588994E-4 54-55 0.0 0.0 0.0 0.06632858678130038 2.9633024623067927E-4 56-57 9.877674874355975E-5 0.0 0.0 0.096702437019945 2.9633024623067927E-4 58-59 9.877674874355975E-5 0.0 0.0 0.1288542687359737 2.9633024623067927E-4 60-61 9.877674874355975E-5 0.0 0.0 0.1577958561178367 2.9633024623067927E-4 62-63 9.877674874355975E-5 0.0 0.0 0.1938987577836078 3.95106994974239E-4 64-65 9.877674874355975E-5 0.0 0.0 0.2371135853589152 3.95106994974239E-4 66-67 9.877674874355975E-5 0.0 0.0 0.3070475234693555 3.95106994974239E-4 68-69 9.877674874355975E-5 0.0 0.0 0.39066204128077886 3.95106994974239E-4 70-71 9.877674874355975E-5 0.0 0.0 0.46425071909473087 3.95106994974239E-4 72-73 9.877674874355975E-5 0.0 0.0 0.5467293042956033 3.95106994974239E-4 74-75 9.877674874355975E-5 0.0 0.0 0.6406166039763568 3.95106994974239E-4 76-77 9.877674874355975E-5 0.0 0.0 0.7519873881847204 3.95106994974239E-4 78-79 9.877674874355975E-5 0.0 0.0 0.8549621487498815 3.95106994974239E-4 80-81 2.9633024623067927E-4 0.0 0.0 0.9521090811391725 3.95106994974239E-4 82-83 2.9633024623067927E-4 0.0 0.0 1.0404648828902867 3.95106994974239E-4 84-85 3.4571862060245914E-4 0.0 0.0 1.179937652116193 3.95106994974239E-4 86-87 3.95106994974239E-4 0.0 0.0 1.356945585864652 3.95106994974239E-4 88-89 3.95106994974239E-4 0.0 0.0 1.5751928122135475 3.95106994974239E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2675 0.0 42.67391 1 GTATCAA 4325 0.0 35.19159 1 ATCAACG 5280 0.0 28.369001 3 TATCAAC 5315 0.0 28.182186 2 TCAACGC 5320 0.0 28.155697 4 CAACGCA 5345 0.0 28.024008 5 AACGCAG 5490 0.0 27.520515 6 GTACGGG 70 0.008336479 27.179216 1 GTACATG 3355 0.0 24.526104 1 ACGCAGA 6190 0.0 24.333992 7 TACATGG 3270 0.0 24.28489 2 CGCAGAG 6155 0.0 24.238367 8 ACATGGG 3360 0.0 22.926783 3 GAGTACT 3850 0.0 22.642447 12-13 ATACCGT 105 0.002192863 22.62468 6 GTACTAG 150 3.001555E-5 22.196363 1 GTACCTG 775 0.0 22.094074 1 GCAGAGT 6665 0.0 21.529306 9 GTACTTT 4175 0.0 20.878311 14-15 GTGTAGC 775 0.0 20.866625 1 >>END_MODULE