FastQCFastQC Report
Thu 26 May 2016
SRR937843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937843_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012384
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45700.4514097417580681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41410.40903451654708095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25260.24951006732623193No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11900.1175443310048361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25950.041.936621
TATACGG651.3256048E-436.550025
GTATCAA41850.036.4506151
ATATACG801.0751484E-535.6362654
TAGTGCG753.0787263E-431.665739
ATCAACG51250.029.760633
TCAACGC51550.029.5874314
TATCAAC52100.029.3662872
CAACGCA52300.029.1631415
AACGCAG54350.028.2268416
ACGCAGA61000.024.9951617
GTAGTAC2101.2605597E-924.888823
CGCAGAG62050.024.41918
TACATGG33100.023.5421852
GTACATG33600.023.336731
ACATGGG32450.023.1352023
GAGTACT42250.022.37211812-13
TACCTGG7650.022.360012
GCAGAGT68000.022.0030259
GGCCGCG1302.941763E-421.9224267
Produced by FastQC (version 0.11.5)