FastQCFastQC Report
Thu 26 May 2016
SRR937842_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937842_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007948
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38230.37928543932821934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30600.3035870898101886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19950.197926877180172No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12800.1269906780905364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.045.1593251
GTATCAA40100.038.1795651
ATCAACG51050.030.0788613
TATCAAC51450.029.8450132
TCAACGC51500.029.7281654
CAACGCA51900.029.4059725
AACGCAG54000.028.4158746
CGCAGAG61500.024.0259578
ACGCAGA62400.023.8317077
GTACCTG8300.022.9140241
GAGTACT38200.022.19536612-13
GCAGAGT65350.021.809699
GTACATG33950.021.7075791
TCGCGGA550.002879590321.58899582-83
GTGTAGG3600.021.1318231
TACCTGG8350.021.065412
AGAGTAC55900.020.73367510-11
GTACTTT41100.020.68551414-15
AGTACTT40800.020.43169812-13
TACATGG34750.020.1102352
Produced by FastQC (version 0.11.5)