##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937842_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1007948 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.446000190486018 31.0 31.0 34.0 30.0 34.0 2 31.561523015076176 33.0 31.0 34.0 30.0 34.0 3 31.605664181088706 33.0 31.0 34.0 30.0 34.0 4 35.18751463369142 37.0 35.0 37.0 33.0 37.0 5 35.15786429458662 37.0 35.0 37.0 33.0 37.0 6 35.237850563719554 37.0 35.0 37.0 33.0 37.0 7 35.1686773524031 37.0 35.0 37.0 32.0 37.0 8 35.146724831042874 37.0 35.0 37.0 32.0 37.0 9 36.72715457543445 39.0 37.0 39.0 33.0 39.0 10-11 36.60706157460504 39.0 37.0 39.0 32.0 39.0 12-13 36.46594764809296 39.0 37.0 39.0 32.0 39.0 14-15 37.57085583780116 40.0 37.0 41.0 32.0 41.0 16-17 37.48766950279181 40.0 37.0 41.0 32.0 41.0 18-19 37.42060850361328 40.0 37.0 41.0 32.0 41.0 20-21 37.2345378928278 40.0 37.0 41.0 31.5 41.0 22-23 37.075582768158675 39.5 36.5 41.0 31.0 41.0 24-25 36.91617722342819 39.0 36.0 41.0 31.0 41.0 26-27 37.01295652156659 39.0 37.0 41.0 31.0 41.0 28-29 36.94566336755467 39.0 36.5 41.0 31.0 41.0 30-31 36.72219697841555 39.0 36.0 41.0 30.0 41.0 32-33 36.47545557905765 39.0 35.5 41.0 30.0 41.0 34-35 36.19831479401715 39.0 35.0 40.0 30.0 41.0 36-37 36.19271827514912 39.0 35.0 41.0 30.0 41.0 38-39 36.12751600280967 39.0 35.0 41.0 29.5 41.0 40-41 35.9242336906269 39.0 35.0 41.0 28.5 41.0 42-43 35.67013625702913 39.0 35.0 40.5 27.0 41.0 44-45 35.43744915412303 39.0 35.0 40.0 26.5 41.0 46-47 35.08857847825483 38.5 35.0 40.0 24.5 41.0 48-49 34.747781135534765 38.0 34.0 40.0 24.0 41.0 50-51 33.865062979439415 37.5 33.5 39.5 23.0 40.5 52-53 33.97300555187371 38.0 33.5 39.5 23.0 40.5 54-55 34.25010863655665 38.0 34.0 40.0 23.0 41.0 56-57 33.94797350656978 38.0 33.5 40.0 21.0 41.0 58-59 33.698585641322765 38.0 33.0 40.0 20.0 41.0 60-61 33.36955477861953 37.5 33.0 40.0 18.5 41.0 62-63 32.9705887605313 37.0 32.0 40.0 16.5 41.0 64-65 32.585702337819015 36.5 32.0 39.0 13.0 41.0 66-67 32.15606211828388 36.0 31.0 39.0 9.0 40.5 68-69 31.65904987162036 35.0 31.0 39.0 7.5 40.0 70-71 31.18858859782449 35.0 30.5 38.0 4.5 40.0 72-73 30.69458989947894 35.0 30.0 37.5 2.0 39.5 74-75 30.18795513260605 34.5 29.0 37.0 2.0 39.0 76-77 29.694831479401714 34.0 29.0 36.5 2.0 39.0 78-79 29.143866548671163 34.0 28.5 36.0 2.0 38.5 80-81 28.585768809502078 34.0 27.5 35.5 2.0 37.0 82-83 28.032391055887803 34.0 26.5 35.0 2.0 37.0 84-85 27.528381424438564 33.0 26.0 35.0 2.0 36.0 86-87 26.96565050974852 33.0 25.5 35.0 2.0 36.0 88-89 26.430772222376554 32.5 24.5 35.0 2.0 35.5 90-91 25.879152992019428 32.0 23.0 35.0 2.0 35.0 92-93 25.26683271359237 32.0 20.0 34.0 2.0 35.0 94-95 24.583859484814692 31.5 15.5 34.0 2.0 35.0 96-97 23.747351053824204 31.0 4.5 34.0 2.0 35.0 98-99 22.735985884192438 31.0 2.0 34.0 2.0 35.0 100-101 20.247458202208847 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4754854839732481 1101 2 0.5200583575633786 1101 3 0.48889909988985636 1101 4 0.5590730703174387 1101 5 0.43003819255527986 1101 6 0.4150439069642502 1101 7 0.4294003077349231 1101 8 0.489317392234355 1101 9 0.5678987678976242 1101 10-11 0.5108281508684698 1101 12-13 0.5413995536044709 1101 14-15 0.869168391712428 1101 16-17 0.9602501618352761 1101 18-19 0.8531471662555177 1101 20-21 0.8244906755990584 1101 22-23 0.921624612048447 1101 24-25 0.9197026048927981 1101 26-27 1.1413393902647613 1101 28-29 0.9380943698362003 1101 30-31 0.5972091133338395 1101 32-33 0.7136115242525491 1101 34-35 0.8446453853768787 1101 36-37 0.6839625340658131 1101 38-39 0.565828305851241 1101 40-41 0.8808478967656796 1101 42-43 0.7915406632548425 1101 44-45 0.98771015489951 1101 46-47 0.9984304971659981 1101 48-49 0.9543935854098606 1101 50-51 1.0758471660676818 1101 52-53 0.9579507194123238 1101 54-55 1.0350888242281329 1101 56-57 1.2022291428588048 1101 58-59 1.1877076937261393 1101 60-61 1.210720446546226 1101 62-63 1.2220244783292173 1101 64-65 1.2095068342366346 1101 66-67 1.39882159345634 1101 68-69 1.3532206759473517 1101 70-71 1.5368016649038339 1101 72-73 1.540945408145987 1101 74-75 1.4271947319318912 1101 76-77 1.3764507815591749 1101 78-79 1.3715118789967846 1101 80-81 1.442750410945301 1101 82-83 1.5370840382229254 1101 84-85 1.7924896212452666 1101 86-87 2.0753486559412515 1101 88-89 2.204604677689801 1101 90-91 2.0461471916961678 1101 92-93 2.1585497789542103 1101 94-95 2.23390872593502 1101 96-97 2.118998017968817 1101 98-99 2.2169298345899016 1101 100-101 2.396833719816321 1102 1 0.3951140588530926 1102 2 0.2841977325793934 1102 3 0.3242743501726224 1102 4 0.3019544243883985 1102 5 0.2519128150721244 1102 6 0.3452237079093763 1102 7 0.31157431785970857 1102 8 0.22891166876615898 1102 9 0.29162319512985135 1102 10-11 0.362504267578025 1102 12-13 0.3705676081889919 1102 14-15 0.4020478606554079 1102 16-17 0.6458794468410076 1102 18-19 0.6011206536690921 1102 20-21 0.5048823246650969 1102 22-23 0.6385406158699496 1102 24-25 0.6819934563483727 1102 26-27 0.7262584578768028 1102 28-29 0.7030495660176257 1102 30-31 0.7015600985419965 1102 32-33 0.5290574942881605 1102 34-35 0.6542502039836435 1102 36-37 0.5906907937815475 1102 38-39 0.7226979501660225 1102 40-41 0.669245025514428 1102 42-43 0.7712705042818158 1102 44-45 0.7709758005467009 1102 46-47 0.8976777271259948 1102 48-49 0.8128492995439274 1102 50-51 0.7038871637249926 1102 52-53 0.7732154975345935 1102 54-55 0.7778621952396279 1102 56-57 0.8851130958440834 1102 58-59 0.914132612555818 1102 60-61 0.7903030462658407 1102 62-63 0.9242296205191138 1102 64-65 0.9207674540960085 1102 66-67 0.8783455345817828 1102 68-69 1.0197731062982847 1102 70-71 1.3219478629850343 1102 72-73 1.2394777536478472 1102 74-75 1.1377200603651119 1102 76-77 1.1457079130166505 1102 78-79 1.1551220638125343 1102 80-81 1.2240508564211794 1102 82-83 1.2443767382020567 1102 84-85 1.5281155203415047 1102 86-87 1.553825710456227 1102 88-89 1.4535042571049388 1102 90-91 1.5864623924165464 1102 92-93 1.8567645810523778 1102 94-95 1.920315855013211 1102 96-97 1.884751648212859 1102 98-99 1.8481301669580965 1102 100-101 1.952229486880391 1103 1 0.31216970428631896 1103 2 0.1253239018836645 1103 3 0.2979008320491161 1103 4 0.21770579118147992 1103 5 0.39153314401176686 1103 6 0.3788801588317483 1103 7 0.46393906880594926 1103 8 0.4327561176729162 1103 9 0.388001524971223 1103 10-11 0.4072485077244252 1103 12-13 0.4619267858777789 1103 14-15 0.6629589992139344 1103 16-17 0.5228254457271149 1103 18-19 0.6118480376479667 1103 20-21 0.5776534005576295 1103 22-23 0.5642488287524756 1103 24-25 0.5837131718886965 1103 26-27 0.4499270896439853 1103 28-29 0.597531340215447 1103 30-31 0.44634866711275833 1103 32-33 0.48181362354529966 1103 34-35 0.3410386285817495 1103 36-37 0.4862632556003774 1103 38-39 0.602757831426473 1103 40-41 0.5524434160516378 1103 42-43 0.37439897335989514 1103 44-45 0.2511847692856932 1103 46-47 0.5062018558072481 1103 48-49 0.39515666980751263 1103 50-51 0.21414190061495475 1103 52-53 0.28949388003964316 1103 54-55 0.5470948588475082 1103 56-57 0.4930253407188516 1103 58-59 0.4292999428859332 1103 60-61 0.4944231270420758 1103 62-63 0.3194151558132603 1103 64-65 0.6028114112224614 1103 66-67 0.8422969589639351 1103 68-69 0.8329472677522567 1103 70-71 0.6943393057047516 1103 72-73 0.6531803515966175 1103 74-75 0.6944675474738773 1103 76-77 0.4858030675788818 1103 78-79 0.46207651162055186 1103 80-81 0.4253729422061525 1103 82-83 0.3074272915496117 1103 84-85 0.348493050755728 1103 86-87 0.40578345448041375 1103 88-89 0.36524664833927645 1103 90-91 0.5167824663730265 1103 92-93 0.7370456308403952 1103 94-95 0.5862680257799404 1103 96-97 0.6750005314775542 1103 98-99 0.6874670970821022 1103 100-101 1.0229593714370537 1104 1 0.4403418603065745 1104 2 0.555558365029519 1104 3 0.4924171455527713 1104 4 0.49408379667341507 1104 5 0.40998314220860266 1104 6 0.5832232138077131 1104 7 0.5567846198793447 1104 8 0.5811917420579107 1104 9 0.6539355909052844 1104 10-11 0.7223389493971482 1104 12-13 0.6610942382097846 1104 14-15 0.8320228467587967 1104 16-17 0.8651957336670222 1104 18-19 0.8999703232386054 1104 20-21 0.9689873830308571 1104 22-23 0.9653065146202877 1104 24-25 1.091506972087565 1104 26-27 1.0132826276830684 1104 28-29 1.236405030987875 1104 30-31 1.0492028218656486 1104 32-33 0.9897825884762952 1104 34-35 0.9019032210919065 1104 36-37 0.9652857333570282 1104 38-39 1.149877144368567 1104 40-41 1.1599476792520989 1104 42-43 1.1498671526255464 1104 44-45 1.1688516780918334 1104 46-47 1.2354610635474614 1104 48-49 1.0741853298488309 1104 50-51 0.911425816886215 1104 52-53 1.0240997336328377 1104 54-55 0.9970806911118189 1104 56-57 1.3306661808913347 1104 58-59 1.2626811889254128 1104 60-61 1.31400654409385 1104 62-63 0.9803250850776166 1104 64-65 1.2399303635312862 1104 66-67 1.4377988316809933 1104 68-69 1.580914584073998 1104 70-71 1.6738197877168801 1104 72-73 1.456327649680624 1104 74-75 1.331595773209699 1104 76-77 1.5579312675551833 1104 78-79 1.4626396147153002 1104 80-81 1.638226427211535 1104 82-83 1.5396065984124654 1104 84-85 1.3575346420758017 1104 86-87 1.2889897933829566 1104 88-89 1.3438039091750191 1104 90-91 1.6890162803789934 1104 92-93 1.7377560783862869 1104 94-95 1.4906110807559045 1104 96-97 1.7531167893406483 1104 98-99 2.055452135953221 1104 100-101 2.0269237614773914 1105 1 0.22182334178805618 1105 2 0.28058889006004506 1105 3 0.31976757290319924 1105 4 0.20222380481342128 1105 5 0.2794174116428678 1105 6 0.2921365227210231 1105 7 0.31759048068520457 1105 8 0.4081351690013335 1105 9 0.24953998650968146 1105 10-11 0.47225754931574926 1105 12-13 0.4419448690604213 1105 14-15 0.6369679017038479 1105 16-17 0.810250678721971 1105 18-19 0.4731082963765729 1105 20-21 0.7931632072066819 1105 22-23 0.7504916998054725 1105 24-25 0.8372741678547655 1105 26-27 0.5053868197872617 1105 28-29 0.7378498824327338 1105 30-31 0.630073802736625 1105 32-33 0.6627984614921658 1105 34-35 0.7236370428257359 1105 36-37 0.5432670113383082 1105 38-39 0.6544965989880254 1105 40-41 0.7887836580880787 1105 42-43 0.4281129808713757 1105 44-45 0.5661451253852832 1105 46-47 0.7362547143411149 1105 48-49 0.7623009179644242 1105 50-51 0.6673273227877203 1105 52-53 0.6498221593552671 1105 54-55 0.5341584281895564 1105 56-57 0.6905562907814442 1105 58-59 0.9103612866055073 1105 60-61 0.877661407748711 1105 62-63 0.9505530053055367 1105 64-65 0.7275575511584691 1105 66-67 0.9771557710379355 1105 68-69 1.0311180842775052 1105 70-71 1.03472739862449 1105 72-73 0.9872393305923062 1105 74-75 1.1877211293350527 1105 76-77 1.0421600017839197 1105 78-79 1.05301405508974 1105 80-81 1.1574775464626548 1105 82-83 1.2007991096049757 1105 84-85 1.3833588178999747 1105 86-87 1.3509149053080698 1105 88-89 0.968244283615391 1105 90-91 1.2779662193289312 1105 92-93 1.508675899306109 1105 94-95 1.4291947193395451 1105 96-97 1.449148535372391 1105 98-99 1.655696336197419 1105 100-101 1.70879189334552 1106 1 0.23378271269238127 1106 2 0.49578699470769294 1106 3 0.4442811327378564 1106 4 0.386080544404102 1106 5 0.3755964619503587 1106 6 0.2995440234771465 1106 7 0.3570576648771606 1106 8 0.19488282501504983 1106 9 0.37873899850686144 1106 10-11 0.13436688493425208 1106 12-13 -0.09808310755012428 1106 14-15 0.08564342342983622 1106 16-17 0.16396703117685263 1106 18-19 0.2406340046132911 1106 20-21 0.2500653646822286 1106 22-23 0.05203167359314875 1106 24-25 0.1232997248665626 1106 26-27 0.3327938164603168 1106 28-29 0.5180885657677408 1106 30-31 0.6895946717758434 1106 32-33 0.6792466921605822 1106 34-35 0.5341207105341752 1106 36-37 0.5178183774034366 1106 38-39 0.7223008131225157 1106 40-41 0.8326401456372281 1106 42-43 0.8537456051278483 1106 44-45 0.9954474744124013 1106 46-47 1.0242461953371844 1106 48-49 1.252216736091242 1106 50-51 1.0243365979162604 1106 52-53 1.412541435491974 1106 54-55 1.285664376369816 1106 56-57 1.1200174259765205 1106 58-59 1.0293715194918889 1106 60-61 0.8980855936545495 1106 62-63 1.125450928483275 1106 64-65 1.3521051366005565 1106 66-67 1.3583748959497157 1106 68-69 1.4369276416925594 1106 70-71 1.4952022448791524 1106 72-73 1.6278041556754772 1106 74-75 1.5082801574628917 1106 76-77 1.5875942693512144 1106 78-79 1.6348078347046204 1106 80-81 1.4869109570887247 1106 82-83 1.484577716319361 1106 84-85 1.579520225299916 1106 86-87 1.4630620471570452 1106 88-89 1.8429216704487423 1106 90-91 1.6982051547054802 1106 92-93 1.5690209441694982 1106 94-95 1.4490190463886385 1106 96-97 1.4671531225283552 1106 98-99 1.8674515534572222 1106 100-101 1.6297252031549796 1107 1 0.47424078386067237 1107 2 0.4718875020483111 1107 3 0.494168650752556 1107 4 0.32083074066173367 1107 5 0.41611341040783145 1107 6 0.49396942800220245 1107 7 0.6235242659293405 1107 8 0.47964093016226883 1107 9 0.45028662173907463 1107 10-11 0.6636817700503101 1107 12-13 0.7171059149111159 1107 14-15 0.9839841676856338 1107 16-17 0.9042032140538083 1107 18-19 0.9353105896133584 1107 20-21 1.0046195669388993 1107 22-23 0.9745201617649641 1107 24-25 0.8872376781630962 1107 26-27 0.9866451527697322 1107 28-29 1.0185433269192785 1107 30-31 0.8126401508202221 1107 32-33 0.8345919298373587 1107 34-35 1.003253339683404 1107 36-37 0.7100585850264309 1107 38-39 0.7402803554614366 1107 40-41 1.088738045973507 1107 42-43 1.1782561327386603 1107 44-45 0.9807838072653468 1107 46-47 0.9849045957496116 1107 48-49 0.9766666288818513 1107 50-51 0.8976874479754358 1107 52-53 0.8375644298561582 1107 54-55 0.9234262484918574 1107 56-57 0.8587267537794929 1107 58-59 1.1093150148695798 1107 60-61 1.0817131669728823 1107 62-63 1.0833719743313992 1107 64-65 0.8888265503585018 1107 66-67 1.038802298201702 1107 68-69 1.1491685523279607 1107 70-71 0.9742759088626585 1107 72-73 1.117228945064678 1107 74-75 1.2379997140963965 1107 76-77 1.141930537761347 1107 78-79 1.3203115723872543 1107 80-81 1.566524183095499 1107 82-83 1.6242809279143806 1107 84-85 1.5016689241411179 1107 86-87 1.3767475018303195 1107 88-89 1.5806584977537455 1107 90-91 1.602407997218986 1107 92-93 1.8662362827285577 1107 94-95 1.2428643192160393 1107 96-97 1.3432098380543849 1107 98-99 1.829307271877319 1107 100-101 1.8483979450205403 1108 1 0.17379378513781063 1108 2 0.26289279305360225 1108 3 0.34830110833328476 1108 4 0.2280100874962585 1108 5 0.23621923051365457 1108 6 0.2849747190074936 1108 7 0.16302435715356722 1108 8 0.23164786320368336 1108 9 0.5339848519890324 1108 10-11 0.48467702639039345 1108 12-13 0.5311453237781194 1108 14-15 0.5091164428179056 1108 16-17 0.43551593071136097 1108 18-19 0.650599770419781 1108 20-21 0.6957115209618863 1108 22-23 0.6769695323177842 1108 24-25 0.9502052023375214 1108 26-27 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0.6211922342446101 2310 58-59 0.5401639446804154 2310 60-61 0.5464146440412136 2310 62-63 0.5769745767532868 2310 64-65 0.4725309895874119 2310 66-67 0.5874054689839667 2310 68-69 0.5295253489151257 2310 70-71 0.5334405528350281 2310 72-73 0.5462290319903857 2310 74-75 0.7546476583034689 2310 76-77 0.8274082613777267 2310 78-79 1.1074940428158015 2310 80-81 1.3681012159868473 2310 82-83 1.1153676998280133 2310 84-85 1.2346849786502219 2310 86-87 1.1368526150677702 2310 88-89 1.3890073774151332 2310 90-91 1.5966993186903018 2310 92-93 1.5210409339224498 2310 94-95 1.6034923240036427 2310 96-97 2.119829163644642 2310 98-99 2.210359474106628 2310 100-101 2.409060385760611 2311 1 -0.046512728272151804 2311 2 -0.7813053459721218 2311 3 -0.15429878219763893 2311 4 -0.054009616937243266 2311 5 0.248808174137082 2311 6 0.07506776772122947 2311 7 -0.046335248757763736 2311 8 0.08276992122229387 2311 9 -0.10367250980626608 2311 10-11 0.09982480964162477 2311 12-13 0.09976875016016606 2311 14-15 0.016231932692015505 2311 16-17 0.05586349502443966 2311 18-19 0.1621624414652061 2311 20-21 0.16218661674732715 2311 22-23 -0.06235723287104378 2311 24-25 0.08710996424228057 2311 26-27 -0.46418498771557637 2311 28-29 -0.5101923052646313 2311 30-31 -0.19677875084006047 2311 32-33 -0.04775162060963822 2311 34-35 0.3460055746770294 2311 36-37 0.04658476638019948 2311 38-39 0.2192075585265698 2311 40-41 0.23896154533493785 2311 42-43 0.1389841020873277 2311 44-45 0.13946835388092182 2311 46-47 0.1177270733996778 2311 48-49 0.22570877447572002 2311 50-51 0.34324938922702586 2311 52-53 0.30579559205283147 2311 54-55 0.42821552052250667 2311 56-57 -0.6821417008820632 2311 58-59 -0.7082137207626076 2311 60-61 0.10677702393329724 2311 62-63 0.36801437535311265 2311 64-65 0.49227482622058005 2311 66-67 0.6722633454558533 2311 68-69 0.7979552700802053 2311 70-71 0.6130051553505247 2311 72-73 0.7921970165844741 2311 74-75 0.7167672747605103 2311 76-77 0.5557236412096245 2311 78-79 0.7998773450564798 2311 80-81 0.8546787169396879 2311 82-83 0.9837956150152714 2311 84-85 0.9501044286111053 2311 86-87 1.39159424512534 2311 88-89 1.5840109881752014 2311 90-91 1.2947770904501468 2311 92-93 1.358413805078495 2311 94-95 1.5904058262403034 2311 96-97 1.8618879989739234 2311 98-99 2.0205588683013325 2311 100-101 2.5497070127089145 2312 1 0.09678590542986498 2312 2 -0.13099449510824712 2312 3 0.0010724315665484596 2312 4 0.13557326457797814 2312 5 0.373365392383306 2312 6 0.20876978463730467 2312 7 0.20194784240106145 2312 8 -0.01766020962423198 2312 9 -0.2947915729162176 2312 10-11 0.057968379366194256 2312 12-13 0.17571937547643302 2312 14-15 0.06475719836107174 2312 16-17 0.14288366418514187 2312 18-19 0.2895480411182092 2312 20-21 0.2836624354417552 2312 22-23 0.20769445512143392 2312 24-25 0.07653742032555755 2312 26-27 -0.05742217415380679 2312 28-29 0.061357611600833195 2312 30-31 0.05160374030430148 2312 32-33 0.28803056691295126 2312 34-35 0.11918384403291071 2312 36-37 0.17320227523583753 2312 38-39 0.1983697070120769 2312 40-41 0.1973490968422027 2312 42-43 0.3824417757662104 2312 44-45 0.26879677143315206 2312 46-47 0.39884830523659076 2312 48-49 0.26745676840329224 2312 50-51 0.30801661253361345 2312 52-53 0.2702013608665368 2312 54-55 0.3901724351469582 2312 56-57 0.4648979868212493 2312 58-59 0.3671585773650605 2312 60-61 0.4818312421072264 2312 62-63 0.5178462990139252 2312 64-65 0.6875144705493597 2312 66-67 0.6738175997752336 2312 68-69 0.6074145341588597 2312 70-71 0.6725818921393625 2312 72-73 0.7091528468454449 2312 74-75 0.9041723929561272 2312 76-77 0.8674600685934166 2312 78-79 0.9930928442251314 2312 80-81 1.1229773506417047 2312 82-83 1.3798232301385305 2312 84-85 1.651859290174034 2312 86-87 1.767750899502552 2312 88-89 1.674257138684851 2312 90-91 1.7703879485431138 2312 92-93 2.052885358609906 2312 94-95 2.1069333329272126 2312 96-97 2.2388759986847617 2312 98-99 2.4586417136710956 2312 100-101 3.2534137285333955 2313 1 -0.04219782223310631 2313 2 -0.35661094713979224 2313 3 -0.21869396555834086 2313 4 -0.050889748300129156 2313 5 0.21104134326679969 2313 6 0.1853396159241143 2313 7 0.19276345585818433 2313 8 -0.018808257942090734 2313 9 0.2295969665666604 2313 10-11 0.2530267800849728 2313 12-13 0.35633233344788096 2313 14-15 0.31985353458948396 2313 16-17 0.3837142521855412 2313 18-19 0.3523512756195615 2313 20-21 0.4943842084276824 2313 22-23 0.5161001654139383 2313 24-25 0.2184910990716915 2313 26-27 0.09158950598995119 2313 28-29 0.22159021617306252 2313 30-31 0.13862081128363712 2313 32-33 0.33158153027523696 2313 34-35 0.31407253326121776 2313 36-37 0.4181957862670842 2313 38-39 0.5556972501886719 2313 40-41 0.734021753326175 2313 42-43 0.5466925494509454 2313 44-45 0.4974231066632697 2313 46-47 0.5158843439707397 2313 48-49 0.21704247270596966 2313 50-51 0.4659231713835723 2313 52-53 0.36325317278627267 2313 54-55 0.6991970932282143 2313 56-57 0.6719360221611765 2313 58-59 0.7150621413063618 2313 60-61 0.7468107768980801 2313 62-63 0.5813832861358179 2313 64-65 0.8658562894572128 2313 66-67 0.9071110009931616 2313 68-69 0.8502267533861705 2313 70-71 0.8161478300449261 2313 72-73 0.9663805597335369 2313 74-75 0.8432359848499118 2313 76-77 0.8447037543276714 2313 78-79 0.9483539004295842 2313 80-81 0.892723781708888 2313 82-83 0.849659508465372 2313 84-85 0.9612383457795062 2313 86-87 0.9038046081977704 2313 88-89 1.1157615811326878 2313 90-91 1.0482755396382508 2313 92-93 1.1541581947884048 2313 94-95 0.9673306574754825 2313 96-97 1.3562913925152493 2313 98-99 0.9850817655828479 2313 100-101 1.483537518091147 2314 1 -0.1919501357304405 2314 2 -0.2973980632257138 2314 3 -0.18175600312236995 2314 4 -0.054181343822804706 2314 5 -0.06246779478416897 2314 6 -0.31058883956609407 2314 7 -0.40108864895009333 2314 8 -0.035672019825774726 2314 9 -0.07637175115504391 2314 10-11 0.01724135037524377 2314 12-13 -0.04694134432779151 2314 14-15 -0.01235677760090681 2314 16-17 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2314 82-83 0.8106815578340338 2314 84-85 0.9246945737775647 2314 86-87 1.134557258970343 2314 88-89 1.2877935590411624 2314 90-91 1.248842056639031 2314 92-93 1.1627081220634317 2314 94-95 1.0501854815653644 2314 96-97 1.0043278182409203 2314 98-99 1.1583658778111818 2314 100-101 1.4456061746817142 2315 1 -0.44768501775506664 2315 2 -0.6392842842743107 2315 3 -0.5356613550633895 2315 4 -0.20830298010502446 2315 5 -0.20305492482714982 2315 6 -0.40187491196248715 2315 7 -0.44164831065091903 2315 8 -0.48781939786864115 2315 9 -0.5046805322531327 2315 10-11 -0.5585441518799357 2315 12-13 -0.5010784459791182 2315 14-15 -0.5119764910991762 2315 16-17 -0.5124238657370483 2315 18-19 -0.6905260993551536 2315 20-21 -0.5548828371313732 2315 22-23 -0.6315808047467542 2315 24-25 -0.44093308260045916 2315 26-27 -0.5567450228776067 2315 28-29 -0.41930379818560937 2315 30-31 -0.42804953394986 2315 32-33 -0.3906529538364154 2315 34-35 -0.41732280844698266 2315 36-37 -0.5979169495443841 2315 38-39 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-0.7096251287451452 2316 60-61 -0.49940843190390893 2316 62-63 -0.40316943119835713 2316 64-65 -0.3722617244886166 2316 66-67 -0.7388401535672635 2316 68-69 -0.6139538415891259 2316 70-71 -0.490509726281509 2316 72-73 -0.2473287503333914 2316 74-75 -0.4002273460306398 2316 76-77 -0.5983513632307584 2316 78-79 -0.5175594111790929 2316 80-81 -0.42556347432670805 2316 82-83 -0.782824110442089 2316 84-85 -0.7821689214688234 2316 86-87 -0.6849230977484773 2316 88-89 -0.6901478761012712 2316 90-91 -0.5255427441138032 2316 92-93 -0.5787388934066975 2316 94-95 -0.5578173855533564 2316 96-97 -0.21023336308235585 2316 98-99 -0.21546660681982743 2316 100-101 0.29828926131970945 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5700.0 3 1462.0 4 1368.0 5 1406.0 6 1573.0 7 1815.0 8 2108.0 9 2781.0 10 3309.0 11 3997.0 12 4911.0 13 5977.0 14 6802.0 15 7930.0 16 9308.0 17 11066.0 18 9947.0 19 8095.0 20 8132.0 21 8519.0 22 9317.0 23 10346.0 24 11371.0 25 12968.0 26 14902.0 27 16895.0 28 20035.0 29 23607.0 30 28556.0 31 34964.0 32 43129.0 33 54474.0 34 72444.0 35 98622.0 36 137386.0 37 174236.0 38 124441.0 39 14049.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.733658984212106 18.66232332792904 14.78277832351504 22.821239364343818 2 15.237681205556639 19.69594473603578 41.373362037062826 23.69301202134475 3 19.349871378862364 24.108555046464758 29.78976959607193 26.751803978600947 4 12.651211281953332 16.51635071467227 37.43155699869481 33.40088100467959 5 13.631366728115827 37.0147448135484 34.57674197421921 14.77714648411657 6 31.794028025629682 35.99290163192874 17.785985196820647 14.427085145620932 7 28.249440098769263 32.56085353500712 20.825045403442015 18.364660962781603 8 25.885754956867846 35.3490831807455 19.83264047523902 18.932521387147634 9 25.696492855374647 17.350707503323022 20.69639840892529 36.25640123237704 10-11 24.647814163783252 26.661092865916125 27.55159203755058 21.13950093275004 12-13 25.57281617096658 24.067076825809607 28.143517924829343 22.216589078394474 14-15 22.4822215252383 25.563525583258734 26.48665924726731 25.46759364423566 16-17 21.76632430836699 28.84939382403254 27.21395621140269 22.170325656197782 18-19 21.680403886181868 27.21018144660277 29.77413108607531 21.33528358114005 20-21 22.298114267113604 26.300917148515442 30.09121788675663 21.309750697614323 22-23 22.046841405798816 26.262309486865735 29.918402279050486 21.77244682828496 24-25 21.884133961874987 26.438857591578802 30.010064287187543 21.66694415935867 26-27 21.63367082152721 26.685755267961913 29.886069777601726 21.794504132909147 28-29 21.6076540373526 26.82094302567699 29.947693633714266 21.623709303256135 30-31 21.67785625205968 26.584556135400362 29.88138724680529 21.85620036573467 32-33 21.49072509629824 26.529997479767303 29.9321127851355 22.047164638798957 34-35 22.085974344088243 26.728155494230204 29.64357289984969 21.542297261831862 36-37 21.964658354317965 26.447243720389807 29.632385061626977 21.955712863665255 38-39 21.719906481451577 26.80737514503574 29.624112083123183 21.8486062903895 40-41 21.983182053304038 26.698825784735646 29.612525360336655 21.705466801623658 42-43 21.773618024522513 26.594827928945154 29.460558435775514 22.170995610756826 44-45 21.838112765135833 26.919844602408695 29.38762872559364 21.854413906861836 46-47 22.051607766660254 27.082174740413063 28.851485906291764 22.01473158663492 48-49 22.04219779258402 27.118389632183497 28.81351888193645 22.025893693296034 50-51 22.05142760003539 27.422337186194913 28.624074105370312 21.902161108399383 52-53 22.200585726508177 27.23439351057584 28.556578386781332 22.008442376134653 54-55 22.00823819189182 27.280064728650576 28.614148856016524 22.097548223441073 56-57 22.142409580962102 27.530508210968396 28.45553098125656 21.87155122681294 58-59 22.079267598831077 27.23283919244662 28.569320266004254 22.11857294271805 60-61 22.019609421346185 27.273233149799324 28.77946406479635 21.927693364058136 62-63 21.872902220819906 27.338370073712635 28.94678131420664 21.841946391260816 64-65 21.847939071484078 27.266136934136544 28.785474225226427 22.100449769152952 66-67 21.722072664287655 27.470513237119388 28.55950368628012 22.24791041231284 68-69 21.83981722472157 27.30881489232934 28.697865269815146 22.15350261313394 70-71 21.898485546381416 27.362374232375885 28.543567559617415 22.195572661625278 72-73 21.88536311716602 27.283252159205308 28.58776892553015 22.243615798098528 74-75 21.87387253074916 27.61560442290968 28.362181637470492 22.148341408870667 76-77 21.995782159049572 27.370001252233646 28.371440327091378 22.2627762616254 78-79 22.0240278204314 27.384768134911997 28.41416981475242 22.17703422990418 80-81 22.034277298184467 27.539753407381678 28.270348512200684 22.155620782233168 82-83 22.029636688007955 27.562358316073336 28.27369049647687 22.134314499441835 84-85 22.02906830878682 27.61787420293167 28.219708919464736 22.133348568816775 86-87 22.06578717911647 27.572496116035524 28.1571650512256 22.20455165362241 88-89 22.093270530248827 27.48419985690436 28.08544995627633 22.337079656570474 90-91 22.06774320011567 27.412796865303136 28.17351536914722 22.345944565433964 92-93 22.101876110802777 27.159609285603203 28.340705974945106 22.39780862864891 94-95 21.980579475537525 27.263661717379346 28.359153001595438 22.39660580548769 96-97 22.06194808111605 27.118931252496864 28.24338989249321 22.575730773893877 98-99 21.94548166936174 27.153664885301197 28.179995766068316 22.72085767926874 100-101 22.217043987721954 26.605233373312835 28.666794521582474 22.510928117382736 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1189.0 1 707.5 2 265.0 3 295.0 4 235.0 5 199.5 6 302.5 7 641.5 8 1104.0 9 1311.0 10 1167.0 11 989.0 12 1004.0 13 1124.0 14 1215.5 15 1279.0 16 1348.0 17 1456.0 18 1588.0 19 1734.0 20 1908.5 21 2271.0 22 2805.0 23 3349.5 24 4048.5 25 5083.0 26 6510.0 27 7962.5 28 9329.5 29 10944.5 30 12708.5 31 14629.0 32 17108.5 33 19032.0 34 20733.0 35 22510.5 36 24674.5 37 27155.0 38 28586.0 39 29603.5 40 30909.0 41 32702.5 42 34157.5 43 35444.0 44 36542.0 45 37402.5 46 37989.0 47 38811.0 48 39898.5 49 39142.5 50 37616.0 51 35963.0 52 34210.5 53 33363.5 54 32281.0 55 29466.5 56 26016.5 57 23090.0 58 20857.0 59 18242.5 60 14771.5 61 11993.5 62 9650.0 63 7307.5 64 5360.0 65 3980.0 66 2953.0 67 2219.5 68 1642.0 69 1172.5 70 869.5 71 633.5 72 456.5 73 366.5 74 283.5 75 199.5 76 146.5 77 104.0 78 64.5 79 43.0 80 24.0 81 20.0 82 16.5 83 8.5 84 10.0 85 10.0 86 5.5 87 4.5 88 2.5 89 2.5 90 2.5 91 2.0 92 2.5 93 1.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31658379202101694 2 0.2714425744185216 3 0.2648946175794783 4 0.27104572854948866 5 0.275907090445142 6 0.20665748629889635 7 0.1951489560969415 8 0.19544659049871618 9 0.2070543321679293 10-11 0.2035323250802621 12-13 0.19812530011468849 14-15 0.20105203839880628 16-17 0.187212038716283 18-19 0.1906844400703211 20-21 0.18259870548877521 22-23 0.18998995979951347 24-25 0.19003956553314258 26-27 0.19222221781282367 28-29 0.20313547921122915 30-31 0.20115124986606453 32-33 0.20720314936881665 34-35 0.2004071638616278 36-37 0.18398766603039046 38-39 0.17168544409036973 40-41 0.16880831153988102 42-43 0.18373963736224488 44-45 0.18686479858087918 46-47 0.18636874124458802 48-49 0.20497089135550645 50-51 0.20130006706695186 52-53 0.2171242960946398 54-55 0.18870021072515647 56-57 0.16890752300713927 58-59 0.17099096381956214 60-61 0.15824229027687936 62-63 0.166228813391167 64-65 0.16409576684511504 66-67 0.16359970950882385 68-69 0.1671713223301202 70-71 0.16642723632568346 72-73 0.16617920765753788 74-75 0.18086250481175617 76-77 0.17322322183287234 78-79 0.17669562318691043 80-81 0.1699988491469798 82-83 0.1514463047696905 84-85 0.1513470933024323 86-87 0.15541476346001976 88-89 0.161516268696401 90-91 0.16493906431681 92-93 0.1624587776353542 94-95 0.16285562350438712 96-97 0.16727053379737844 98-99 0.17768773785949277 100-101 0.1586391361459123 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1007948.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.51217871685657 #Duplication Level Percentage of deduplicated Percentage of total 1 85.81553961682677 61.36856205762001 2 8.941530916296607 12.788567137770023 3 2.285422896822652 4.903067119235326 4 0.9399556805710249 2.6887311445967863 5 0.511300168507307 1.82820945141267 6 0.31591215541871837 1.3554939910278454 7 0.23480364150034266 1.1753923982038856 8 0.16024217876287133 0.9167413860535146 9 0.12336630815038237 0.7939974128480048 >10 0.6287722522868936 7.888613695032415 >50 0.029239871668415834 1.41356805143299 >100 0.01307945478299646 1.723413921766741 >500 2.782861350146573E-4 0.14487817214118576 >1k 5.565722700293146E-4 1.0107640608586297 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3823 0.37928543932821934 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3060 0.3035870898101886 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1995 0.197926877180172 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1280 0.1269906780905364 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.921146725823157E-5 2 0.0 0.0 0.0 0.0 9.921146725823157E-5 3 0.0 0.0 0.0 0.0 9.921146725823157E-5 4 0.0 0.0 0.0 0.0 9.921146725823157E-5 5 0.0 0.0 0.0 0.0 9.921146725823157E-5 6 0.0 0.0 0.0 0.0 9.921146725823157E-5 7 0.0 0.0 0.0 0.0 9.921146725823157E-5 8 0.0 0.0 0.0 0.0 9.921146725823157E-5 9 0.0 0.0 0.0 9.921146725823157E-5 9.921146725823157E-5 10-11 0.0 0.0 0.0 9.921146725823157E-5 9.921146725823157E-5 12-13 0.0 0.0 0.0 9.921146725823157E-5 9.921146725823157E-5 14-15 0.0 0.0 0.0 9.921146725823157E-5 9.921146725823157E-5 16-17 0.0 0.0 0.0 2.9763440177469475E-4 9.921146725823157E-5 18-19 0.0 0.0 0.0 4.4645160266204207E-4 9.921146725823157E-5 20-21 0.0 0.0 0.0 4.960573362911578E-4 9.921146725823157E-5 22-23 4.960573362911579E-5 0.0 0.0 5.456630699202736E-4 9.921146725823157E-5 24-25 9.921146725823157E-5 0.0 0.0 9.425089389531999E-4 9.921146725823157E-5 26-27 9.921146725823157E-5 0.0 0.0 0.0010913261398405472 9.921146725823157E-5 28-29 1.9842293451646315E-4 0.0 0.0 0.001835412144277284 9.921146725823157E-5 30-31 2.9763440177469475E-4 0.0 0.0 0.0039188529567001475 9.921146725823157E-5 32-33 2.9763440177469475E-4 4.960573362911579E-5 0.0 0.00788731164702941 9.921146725823157E-5 34-35 2.9763440177469475E-4 9.921146725823157E-5 0.0 0.011558135935583979 9.921146725823157E-5 36-37 2.9763440177469475E-4 9.921146725823157E-5 0.0 0.014732902887847388 9.921146725823157E-5 38-39 2.9763440177469475E-4 9.921146725823157E-5 0.0 0.018304515709143723 9.921146725823157E-5 40-41 2.9763440177469475E-4 9.921146725823157E-5 0.0 0.021826522796810948 9.921146725823157E-5 42-43 3.472401354038105E-4 9.921146725823157E-5 0.0 0.02415799227737939 9.921146725823157E-5 44-45 4.960573362911578E-4 9.921146725823157E-5 0.0 0.027630393631417494 9.921146725823157E-5 46-47 5.456630699202736E-4 9.921146725823157E-5 0.0 0.03412874473683166 9.921146725823157E-5 48-49 5.952688035493895E-4 9.921146725823157E-5 0.0 0.04062709584224583 9.921146725823157E-5 50-51 5.952688035493895E-4 9.921146725823157E-5 0.0 0.04638136094322326 9.921146725823157E-5 52-53 5.952688035493895E-4 9.921146725823157E-5 0.0 0.056699353538079346 9.921146725823157E-5 54-55 5.952688035493895E-4 9.921146725823157E-5 0.0 0.0729204284348002 9.921146725823157E-5 56-57 5.952688035493895E-4 9.921146725823157E-5 0.0 0.10501533809283811 9.921146725823157E-5 58-59 5.952688035493895E-4 9.921146725823157E-5 0.0 0.13591971014377724 9.921146725823157E-5 60-61 5.952688035493895E-4 9.921146725823157E-5 0.0 0.16846107140447722 1.4881720088734737E-4 62-63 5.952688035493895E-4 9.921146725823157E-5 0.0 0.20779841817236605 1.9842293451646315E-4 64-65 5.952688035493895E-4 9.921146725823157E-5 0.0 0.258346660740435 1.9842293451646315E-4 66-67 6.94480270807621E-4 9.921146725823157E-5 0.0 0.33295368411862514 1.9842293451646315E-4 68-69 6.94480270807621E-4 9.921146725823157E-5 0.0 0.42378178239353614 1.9842293451646315E-4 70-71 6.94480270807621E-4 9.921146725823157E-5 0.0 0.5005218523177783 1.9842293451646315E-4 72-73 6.94480270807621E-4 9.921146725823157E-5 0.0 0.5889192696448626 2.48028668145579E-4 74-75 6.94480270807621E-4 9.921146725823157E-5 0.0 0.6840134610118775 2.9763440177469475E-4 76-77 6.94480270807621E-4 9.921146725823157E-5 0.0 0.7979578311579565 2.9763440177469475E-4 78-79 6.94480270807621E-4 9.921146725823157E-5 0.0 0.9046101584605555 2.9763440177469475E-4 80-81 6.94480270807621E-4 9.921146725823157E-5 0.0 1.0075420557409709 2.9763440177469475E-4 82-83 6.94480270807621E-4 9.921146725823157E-5 0.0 1.1020905840380655 2.9763440177469475E-4 84-85 7.440860044367368E-4 9.921146725823157E-5 0.0 1.2503125161218636 2.9763440177469475E-4 86-87 7.936917380658526E-4 9.921146725823157E-5 0.0 1.422841257683928 2.9763440177469475E-4 88-89 7.936917380658526E-4 9.921146725823157E-5 0.0 1.6501347291725366 2.9763440177469475E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2390 0.0 45.159325 1 GTATCAA 4010 0.0 38.179565 1 ATCAACG 5105 0.0 30.078861 3 TATCAAC 5145 0.0 29.845013 2 TCAACGC 5150 0.0 29.728165 4 CAACGCA 5190 0.0 29.405972 5 AACGCAG 5400 0.0 28.415874 6 CGCAGAG 6150 0.0 24.025957 8 ACGCAGA 6240 0.0 23.831707 7 GTACCTG 830 0.0 22.914024 1 GAGTACT 3820 0.0 22.195366 12-13 GCAGAGT 6535 0.0 21.80969 9 GTACATG 3395 0.0 21.707579 1 TCGCGGA 55 0.0028795903 21.588995 82-83 GTGTAGG 360 0.0 21.131823 1 TACCTGG 835 0.0 21.06541 2 AGAGTAC 5590 0.0 20.733675 10-11 GTACTTT 4110 0.0 20.685514 14-15 AGTACTT 4080 0.0 20.431698 12-13 TACATGG 3475 0.0 20.110235 2 >>END_MODULE