FastQCFastQC Report
Thu 26 May 2016
SRR937842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937842_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007948
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44340.4399036458229988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40220.39902852131260735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25650.25447741351736397No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11330.11240659240357638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23600.051.1498341
GTATCAA41050.038.899911
ATCAACG50300.031.4550913
TCAACGC50950.031.240314
TATCAAC51150.030.9323772
CAACGCA52150.030.5214565
AACGCAG53900.029.5217086
ACGCAGA63900.024.9760577
CGCAGAG64350.024.80148
GAGTACT44500.023.48270412-13
GCAGAGT68900.022.8878149
GTACATG32250.022.2520351
GTACTTT46950.022.20671714-15
TACATGG32700.020.7779662
TAAGGTG5150.020.2969235
AGAGTAC61250.020.24043510-11
CAGAGTA67300.020.18535610-11
AGTACTT47250.019.85412412-13
ACATGGG33150.019.6359423
TGGTATC11400.019.1720142
Produced by FastQC (version 0.11.5)