FastQCFastQC Report
Thu 26 May 2016
SRR937841_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937841_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959651
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38250.39858240131047645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31310.32626444405309846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19420.2023652348614236No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13590.1416139825832516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39450.039.0455931
GGTATCA23700.037.9129371
ATCAACG47850.031.6811733
CGAACGG300.003951332531.66711274-75
TCAACGC47850.031.5802084
CAACGCA48350.031.1553485
TATCAAC49100.030.8762382
AACGCAG50050.030.1841836
TACGGTG700.00838496727.1467847
CGCAGAG55700.027.037128
ACGCAGA56000.026.9771147
GCAGAGT59500.024.9111639
TAGCCCT5550.023.973694
GTACATG30500.023.3811281
GGACCGA1852.537181E-723.1114486
TACATGG30500.022.7493082
GAGTACT35000.022.59851512-13
ACATGGG30850.022.0279143
AGTACTT36400.021.99035512-13
TCTAGAC4200.021.4979383
Produced by FastQC (version 0.11.5)