##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937841_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 959651 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.549196530822144 33.0 31.0 34.0 30.0 34.0 2 31.680193111870878 33.0 31.0 34.0 30.0 34.0 3 31.696521964755938 33.0 31.0 34.0 30.0 34.0 4 35.289793893821816 37.0 35.0 37.0 33.0 37.0 5 35.27923797297142 37.0 35.0 37.0 33.0 37.0 6 35.315994043667956 37.0 35.0 37.0 33.0 37.0 7 35.24991064459892 37.0 35.0 37.0 33.0 37.0 8 35.22742434489205 37.0 35.0 37.0 33.0 37.0 9 36.83113340162205 39.0 37.0 39.0 33.0 39.0 10-11 36.70957150047257 39.0 37.0 39.0 33.0 39.0 12-13 36.572537307833784 39.0 37.0 39.0 32.0 39.0 14-15 37.69964601714582 40.0 38.0 41.0 32.0 41.0 16-17 37.60724680118085 40.0 37.0 41.0 32.0 41.0 18-19 37.53086017729362 40.0 37.0 41.0 32.0 41.0 20-21 37.43360450830562 40.0 37.0 41.0 32.0 41.0 22-23 37.22567110334903 40.0 37.0 41.0 31.5 41.0 24-25 37.047788727360256 39.0 36.5 41.0 31.0 41.0 26-27 36.98333039823853 39.0 36.5 41.0 31.0 41.0 28-29 37.03021046192835 39.0 37.0 41.0 31.0 41.0 30-31 36.840928629262095 39.0 36.0 41.0 30.5 41.0 32-33 36.59133528751598 39.0 36.0 41.0 30.0 41.0 34-35 36.58682739871057 39.0 36.0 41.0 30.0 41.0 36-37 36.480338685626336 39.0 36.0 41.0 30.0 41.0 38-39 36.33513016711284 39.0 35.0 41.0 30.0 41.0 40-41 36.09053343350864 39.0 35.0 41.0 29.5 41.0 42-43 35.826463995765124 39.0 35.0 41.0 28.0 41.0 44-45 35.593756480220414 39.0 35.0 40.5 27.0 41.0 46-47 35.267720244130416 39.0 35.0 40.0 25.5 41.0 48-49 34.90814994201017 38.5 34.5 40.0 24.0 41.0 50-51 33.98704060121857 37.5 33.5 39.5 23.5 40.5 52-53 34.082404957635646 38.0 33.5 39.5 23.0 40.5 54-55 34.413852535974016 38.0 34.0 40.0 23.0 41.0 56-57 34.19280655154843 38.0 34.0 40.0 22.5 41.0 58-59 33.930125639425164 38.0 33.5 40.0 21.5 41.0 60-61 33.58454896623877 38.0 33.0 40.0 19.5 41.0 62-63 33.20008628136687 37.0 33.0 40.0 18.0 41.0 64-65 32.82257091380095 36.5 32.0 39.5 16.0 41.0 66-67 32.396682752375604 36.0 31.5 39.0 12.5 41.0 68-69 31.889790663480788 35.5 31.0 39.0 8.0 40.0 70-71 31.430222028633324 35.0 31.0 38.5 7.0 40.0 72-73 30.95393637895443 35.0 30.0 37.5 4.0 40.0 74-75 30.486437777900505 35.0 30.0 37.0 2.0 39.0 76-77 29.96198826448365 34.0 29.0 36.5 2.0 39.0 78-79 29.41166736657389 34.0 29.0 36.0 2.0 38.5 80-81 28.855918974710598 34.0 28.5 35.5 2.0 37.0 82-83 28.295640290063787 34.0 27.0 35.0 2.0 37.0 84-85 27.795784613364653 33.5 26.0 35.0 2.0 36.5 86-87 27.283855276553666 33.0 26.0 35.0 2.0 36.0 88-89 26.768572116321455 33.0 25.0 35.0 2.0 35.5 90-91 26.233020129192802 32.5 24.5 35.0 2.0 35.0 92-93 25.627437995688016 32.0 21.5 34.5 2.0 35.0 94-95 24.94553228204837 32.0 19.0 34.0 2.0 35.0 96-97 24.198258533571057 31.0 7.5 34.0 2.0 35.0 98-99 23.19454416240904 31.0 2.0 34.0 2.0 35.0 100-101 20.804534669374597 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2833184852474666 1101 2 0.31908730516301276 1101 3 0.38994195460326964 1101 4 0.31146108252674765 1101 5 0.39640115371956597 1101 6 0.26395037445779224 1101 7 0.290737173886761 1101 8 0.40164874892006 1101 9 0.5648056977538687 1101 10-11 0.41962331849413204 1101 12-13 0.5029048622067762 1101 14-15 0.626024433344007 1101 16-17 0.6652174857758126 1101 18-19 0.8330994740343982 1101 20-21 0.9333012979767688 1101 22-23 0.6439971297360145 1101 24-25 0.8061381316679075 1101 26-27 0.8421573365347896 1101 28-29 0.5043554490906885 1101 30-31 0.725975955159285 1101 32-33 0.6265908922283927 1101 34-35 0.7018840042064411 1101 36-37 0.6926353239261971 1101 38-39 0.7554668367378454 1101 40-41 0.8600467537063778 1101 42-43 0.7427522384099419 1101 44-45 0.8532712283763573 1101 46-47 0.9391734878475688 1101 48-49 0.8571083488214057 1101 50-51 0.8981722537633914 1101 52-53 0.9333900174505985 1101 54-55 1.0597116892659457 1101 56-57 1.1870632405867525 1101 58-59 1.3394206843353587 1101 60-61 1.4632660143124383 1101 62-63 1.5109009304825634 1101 64-65 1.3671779165911104 1101 66-67 1.2631386813472503 1101 68-69 1.3631154936480812 1101 70-71 1.5200887979128588 1101 72-73 1.5764920853513047 1101 74-75 1.4820304529993997 1101 76-77 1.5988908830174324 1101 78-79 1.7634805656309105 1101 80-81 1.8035404666084247 1101 82-83 1.6952882950360788 1101 84-85 1.87720230793159 1101 86-87 2.0027795419961905 1101 88-89 2.109542696768198 1101 90-91 2.263960477666938 1101 92-93 2.3126103398379776 1101 94-95 2.2593806718526324 1101 96-97 2.4471560510402703 1101 98-99 2.519837967987147 1101 100-101 2.833711630199833 1102 1 0.2659368226975616 1102 2 0.16429516273862887 1102 3 0.24697384784712284 1102 4 0.22385563908171946 1102 5 0.07676964263537656 1102 6 0.18864472439798163 1102 7 0.2884690717910985 1102 8 0.3455675943628833 1102 9 0.4300342168074778 1102 10-11 0.2707140658803837 1102 12-13 0.37121221334315635 1102 14-15 0.43874289352017826 1102 16-17 0.3528184629239135 1102 18-19 0.4672233419252194 1102 20-21 0.3778153260328807 1102 22-23 0.56458928303487 1102 24-25 0.5299908759160985 1102 26-27 0.4191456154181381 1102 28-29 0.33639841098347034 1102 30-31 0.5144919406185764 1102 32-33 0.30215811193912856 1102 34-35 0.47059778836390365 1102 36-37 0.6228397249763589 1102 38-39 0.6368662634983338 1102 40-41 0.47231198183705203 1102 42-43 0.5264679255982756 1102 44-45 0.6595349185473935 1102 46-47 0.6396597316530119 1102 48-49 0.5381865905762311 1102 50-51 0.6514695460223265 1102 52-53 0.5156718291359752 1102 54-55 0.6040998842605845 1102 56-57 0.602965782103901 1102 58-59 0.5528873463415422 1102 60-61 0.6617569885088841 1102 62-63 0.921609945411646 1102 64-65 0.870314033786741 1102 66-67 0.9010724947104123 1102 68-69 1.0095313874511973 1102 70-71 0.8935809372302579 1102 72-73 0.9629753231447182 1102 74-75 1.056765193798828 1102 76-77 0.8713262576426501 1102 78-79 0.8074900963201976 1102 80-81 0.9703285000396278 1102 82-83 1.0810229426258289 1102 84-85 1.0944148337584387 1102 86-87 1.1766660747516262 1102 88-89 1.204357326026713 1102 90-91 1.3077253329589134 1102 92-93 1.3739500735690058 1102 94-95 1.7075376447130814 1102 96-97 1.7450580566017813 1102 98-99 2.1949818604231837 1102 100-101 2.801376611324873 1103 1 0.4681537565652967 1103 2 0.2064419563258042 1103 3 0.408473931347455 1103 4 0.28379635384457913 1103 5 0.25328099868080756 1103 6 0.25447847523298606 1103 7 0.2161974452045854 1103 8 0.2447931287650178 1103 9 0.21725240318022543 1103 10-11 0.3931213805096405 1103 12-13 0.3526626141447622 1103 14-15 0.5966034062122318 1103 16-17 0.5025721369386034 1103 18-19 0.3200665282979642 1103 20-21 0.29892629797677017 1103 22-23 0.3469404436895118 1103 24-25 0.5822766975593794 1103 26-27 0.434683983821607 1103 28-29 0.3734203715713065 1103 30-31 0.4598180094228539 1103 32-33 0.5214915705229615 1103 34-35 0.46569214374132173 1103 36-37 0.432303444081235 1103 38-39 0.5345366041796993 1103 40-41 0.6529367730862248 1103 42-43 0.6154751066269952 1103 44-45 0.5921157051205412 1103 46-47 0.572143449087875 1103 48-49 0.6905506937826402 1103 50-51 0.6535622731432298 1103 52-53 0.5775518391560226 1103 54-55 0.8061748675605216 1103 56-57 0.7842168258580671 1103 58-59 0.8054090564283811 1103 60-61 0.9633508011341405 1103 62-63 0.9090598452112459 1103 64-65 0.8610611530252186 1103 66-67 0.9137321890991856 1103 68-69 0.9707705324077764 1103 70-71 0.9032888948120821 1103 72-73 0.9026912132582794 1103 74-75 0.8405212475730401 1103 76-77 1.023588072939912 1103 78-79 1.1344471353983536 1103 80-81 1.056871377461139 1103 82-83 1.122504864803517 1103 84-85 1.02297507924942 1103 86-87 1.214262100135727 1103 88-89 1.2980843634348638 1103 90-91 1.1505035978219773 1103 92-93 1.4276418320860422 1103 94-95 1.4532154586743395 1103 96-97 1.5308890936759312 1103 98-99 1.7486412625607883 1103 100-101 2.70575620384324 1104 1 0.17988358208328847 1104 2 0.3430627305897076 1104 3 0.14289436936999422 1104 4 0.10120138837747561 1104 5 0.09807834510029068 1104 6 0.1480728500057964 1104 7 0.11691877381236537 1104 8 0.164737012807187 1104 9 0.1033994860591747 1104 10-11 0.3859035506295925 1104 12-13 0.33128175263985327 1104 14-15 0.5799566186804199 1104 16-17 0.4925144306499831 1104 18-19 0.42439449994463985 1104 20-21 0.667693337235221 1104 22-23 0.4428572693892505 1104 24-25 0.5933624391079064 1104 26-27 0.5518226379473745 1104 28-29 0.5324704897101711 1104 30-31 0.563681377289079 1104 32-33 0.294893856055495 1104 34-35 0.6642222237122439 1104 36-37 0.5315781396464843 1104 38-39 0.5551881854228853 1104 40-41 0.6422707652708795 1104 42-43 0.6740515333809043 1104 44-45 0.6376667547388664 1104 46-47 0.957513541781438 1104 48-49 0.8531103311872172 1104 50-51 0.8779153492973606 1104 52-53 0.9129423789597269 1104 54-55 0.880095714525261 1104 56-57 0.8706745912161225 1104 58-59 0.8177409357450003 1104 60-61 1.0117235519520165 1104 62-63 1.182274130925535 1104 64-65 1.0259415374308887 1104 66-67 1.3245333750315709 1104 68-69 1.511237635279457 1104 70-71 1.2782417528637033 1104 72-73 1.3990169811608624 1104 74-75 1.4472080205646805 1104 76-77 1.577558788134386 1104 78-79 1.37601099853903 1104 80-81 1.4652654841496187 1104 82-83 1.6984721267082818 1104 84-85 1.8380394876463697 1104 86-87 1.9806311477547744 1104 88-89 1.7463338636166164 1104 90-91 1.7259494584181247 1104 92-93 2.00237431572835 1104 94-95 2.1787942254864063 1104 96-97 2.496556691822061 1104 98-99 2.4709979055996882 1104 100-101 2.960593081342335 1105 1 0.29165727769205674 1105 2 0.2206731769830803 1105 3 0.23733249942257473 1105 4 0.09386090783519307 1105 5 0.04136785314089764 1105 6 0.14161169119542194 1105 7 0.181118219852479 1105 8 0.0625014620983535 1105 9 0.182026464213088 1105 10-11 0.366390159852358 1105 12-13 0.39805580663301754 1105 14-15 0.3315872677145748 1105 16-17 0.43153340454423983 1105 18-19 0.30665690388481437 1105 20-21 0.34556655619273613 1105 22-23 0.3242365117646244 1105 24-25 0.3351669557753496 1105 26-27 0.2565516481408565 1105 28-29 0.3570618034961939 1105 30-31 0.47300756341346073 1105 32-33 0.4584707371896286 1105 34-35 0.5224703127554164 1105 36-37 0.47280373750846394 1105 38-39 0.6698065572313396 1105 40-41 0.6225890618186156 1105 42-43 0.5505983460636159 1105 44-45 0.7159199891581025 1105 46-47 0.7521170406371738 1105 48-49 0.8075327947216095 1105 50-51 0.5468647966174132 1105 52-53 0.42662754338137177 1105 54-55 0.6040328487811806 1105 56-57 0.6643738094261451 1105 58-59 0.6024747841279137 1105 60-61 0.5720729255472818 1105 62-63 0.6565363710328427 1105 64-65 0.7076353901144188 1105 66-67 0.7736133510710133 1105 68-69 0.7153714713749082 1105 70-71 0.5836806201641984 1105 72-73 0.7940717883756498 1105 74-75 0.8988472452256246 1105 76-77 0.954258554160571 1105 78-79 1.211038978121362 1105 80-81 1.2325242530479947 1105 82-83 1.389032153535915 1105 84-85 1.5190504900475403 1105 86-87 1.6243266541263388 1105 88-89 1.6561976263456621 1105 90-91 1.902110111906481 1105 92-93 1.9591192381893272 1105 94-95 2.023704015012367 1105 96-97 2.040711570201754 1105 98-99 2.528497483218068 1105 100-101 2.6079569080685907 1106 1 0.0499919918594145 1106 2 -0.07024922014478463 1106 3 0.03824108821020289 1106 4 0.11352674600144042 1106 5 0.1608790378964926 1106 6 -0.08560730908074277 1106 7 -0.01445206459933246 1106 8 -0.20279265554870562 1106 9 0.20898034435669643 1106 10-11 -0.14121685478447432 1106 12-13 -0.19564620938465538 1106 14-15 -0.06318213300345832 1106 16-17 -0.03907001992413939 1106 18-19 0.012640057709731423 1106 20-21 0.3160954156238276 1106 22-23 0.1283803946698967 1106 24-25 0.003636991677026913 1106 26-27 -0.029439790688194023 1106 28-29 0.2826238029438599 1106 30-31 0.32541849961892666 1106 32-33 0.32788250189550894 1106 34-35 0.4037558692315173 1106 36-37 0.19781202251260765 1106 38-39 0.5167424865326424 1106 40-41 0.33884976328229754 1106 42-43 0.3513329497642488 1106 44-45 0.33823702864995653 1106 46-47 0.5872537432055225 1106 48-49 0.6213411349591169 1106 50-51 0.3090340868687278 1106 52-53 0.5799415450383734 1106 54-55 0.4032582008938519 1106 56-57 0.1714533454659133 1106 58-59 0.44707572309504684 1106 60-61 0.5869721246635464 1106 62-63 0.9289127863877127 1106 64-65 0.6921824765546347 1106 66-67 0.7373167479227192 1106 68-69 0.7183195520156218 1106 70-71 0.6952312967728709 1106 72-73 0.6779546936504346 1106 74-75 0.6442283554161783 1106 76-77 0.6915231219595199 1106 78-79 1.0528478706924709 1106 80-81 0.8219387794219273 1106 82-83 0.5446065804897913 1106 84-85 0.660199343955302 1106 86-87 0.8398748452337657 1106 88-89 0.9623368144152558 1106 90-91 1.0216861958611894 1106 92-93 1.0550499203213342 1106 94-95 1.227008350831202 1106 96-97 1.0987383583818122 1106 98-99 1.560614301776475 1106 100-101 2.1769571842353983 1107 1 0.3421408514032329 1107 2 0.44912653015533266 1107 3 0.4472206800469358 1107 4 0.24008136785018053 1107 5 0.31213954209817274 1107 6 0.263108177113736 1107 7 0.43114217309574343 1107 8 0.3875314741031559 1107 9 0.21702356164360737 1107 10-11 0.34637018002944586 1107 12-13 0.31895162975100533 1107 14-15 0.6617038213782962 1107 16-17 0.6570764386592955 1107 18-19 0.7040926387421393 1107 20-21 0.6310053796094195 1107 22-23 0.506363587947213 1107 24-25 0.6528686843541038 1107 26-27 0.682565011232569 1107 28-29 0.6150607777337811 1107 30-31 0.6359347061015228 1107 32-33 0.48848274199154673 1107 34-35 0.6831916435412495 1107 36-37 0.6260953358379382 1107 38-39 0.5863823424750123 1107 40-41 0.6352603275079929 1107 42-43 1.0439139821772159 1107 44-45 0.9337592375335078 1107 46-47 0.8070136471671105 1107 48-49 0.7718213270359442 1107 50-51 0.48085881676068 1107 52-53 0.7744793601644275 1107 54-55 0.8159112621183411 1107 56-57 0.9769995639533064 1107 58-59 0.8571597567084908 1107 60-61 1.0181245856479393 1107 62-63 1.0826342749831497 1107 64-65 1.3645594273349388 1107 66-67 1.4835832545253638 1107 68-69 1.4375272350888508 1107 70-71 1.727464189930135 1107 72-73 1.4367542134583573 1107 74-75 1.3969894598579593 1107 76-77 1.6828436501708062 1107 78-79 1.6376377866588605 1107 80-81 1.736152464896115 1107 82-83 1.7206810102616998 1107 84-85 1.789190940593958 1107 86-87 1.9908352293874252 1107 88-89 2.176994677560515 1107 90-91 2.1738110458011697 1107 92-93 2.253705382506208 1107 94-95 2.3996734168576097 1107 96-97 2.516181335572689 1107 98-99 3.1183453942134527 1107 100-101 3.509130053383057 1108 1 0.07012335954296489 1108 2 0.10586813498585457 1108 3 0.02860161951949891 1108 4 0.03364643655756794 1108 5 0.013382322088581589 1108 6 0.18983569194100625 1108 7 0.025536258273241685 1108 8 -0.08452087040785017 1108 9 0.24523782501645286 1108 10-11 0.13969396115480492 1108 12-13 0.1730255173705686 1108 14-15 0.2301706622089199 1108 16-17 0.2686339648955922 1108 18-19 0.32026814120118985 1108 20-21 0.3639774766368191 1108 22-23 0.3577512894297925 1108 24-25 0.45004189607054457 1108 26-27 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-1.1334161778020544 2316 50-51 -1.1089633416108668 2316 52-53 -1.3195280788767647 2316 54-55 -1.3052663892700807 2316 56-57 -1.1758497725025876 2316 58-59 -0.9824324736262611 2316 60-61 -0.9592670267347145 2316 62-63 -0.8356805919472237 2316 64-65 -0.7489200628217745 2316 66-67 -0.6975980704636555 2316 68-69 -0.8739015987397636 2316 70-71 -1.140657484969335 2316 72-73 -1.1375837184357067 2316 74-75 -0.8793814163613867 2316 76-77 -1.0514887713223793 2316 78-79 -1.0798715121426277 2316 80-81 -0.906354373904982 2316 82-83 -1.0694435641582274 2316 84-85 -1.1826003988926566 2316 86-87 -1.2484086648915955 2316 88-89 -1.154323423450382 2316 90-91 -0.9890370442545269 2316 92-93 -0.9182512690068556 2316 94-95 -1.0046824238393235 2316 96-97 -0.6638428052311731 2316 98-99 -0.5861777265102504 2316 100-101 -0.49639543550101806 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5337.0 3 1248.0 4 1166.0 5 1234.0 6 1444.0 7 1622.0 8 1881.0 9 2442.0 10 2969.0 11 3639.0 12 4387.0 13 5414.0 14 6057.0 15 7229.0 16 8685.0 17 10228.0 18 9402.0 19 7375.0 20 7335.0 21 7749.0 22 8481.0 23 9197.0 24 10474.0 25 11741.0 26 13259.0 27 15477.0 28 17924.0 29 21447.0 30 25947.0 31 31568.0 32 39395.0 33 50999.0 34 68182.0 35 93881.0 36 131503.0 37 171584.0 38 126779.0 39 14962.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.722541443278715 18.63622632279294 14.747002359227695 22.894229874700653 2 15.175843894975454 19.647946423812478 41.53283245004666 23.643377231165406 3 19.292956630869693 24.00490714974644 29.94385409798207 26.7582821214018 4 12.53594205805896 16.533242224395465 37.477327248259314 33.453488469286256 5 13.639450522814087 36.926200048478364 34.670702351200674 14.763647077506874 6 31.774663478106497 35.96069298969288 17.82871583872012 14.435927693480508 7 28.138920504975616 32.583979867804146 20.889450436998132 18.387649190222103 8 25.834824137344043 35.387979785637526 19.8717125291242 18.905483547894235 9 25.584370554950986 17.390232745638237 20.728523298584058 36.29687340082671 10-11 24.614527438419426 26.754469330336722 27.51016682575721 21.12083640548664 12-13 25.56405346739693 24.043776052667443 28.13518380918913 22.2569866707465 14-15 22.500738831633065 25.55569015228808 26.537056975144974 25.40651404093388 16-17 21.757260130347653 28.802842126038936 27.25808354906521 22.181814194548206 18-19 21.646070680599568 27.21792584400727 29.785988672599274 21.35001480279389 20-21 22.166824419884946 26.329995369207793 30.14337705351097 21.359803157396293 22-23 21.943488408634966 26.226151815064362 30.025678544711937 21.804681231588734 24-25 21.788033800746405 26.372130828329187 30.110217328546618 21.729618042377787 26-27 21.52631518494193 26.67631847414426 29.988336384112003 21.80902995680181 28-29 21.580438072381263 26.8010460432391 29.963927382709166 21.654588501670474 30-31 21.636461990563692 26.580706741382947 29.905971106270197 21.876860161783167 32-33 21.470565351816877 26.54379232541005 30.001362872329153 21.984279450443925 34-35 21.970626535741996 26.727732589528603 29.743504326967763 21.55813654776164 36-37 21.926678064760313 26.462823507915584 29.72114694404928 21.889351483274822 38-39 21.55512340281131 26.77617823255328 29.75966796747543 21.909030397159974 40-41 21.99206514930048 26.643558154103154 29.658905825850905 21.705470870745458 42-43 21.691433297169286 26.55227828287944 29.602379935531232 22.15390848442005 44-45 21.810060205865216 26.86365658223645 29.455609921124676 21.870673290773652 46-47 21.978495858664697 27.052982466215425 28.966167130875593 22.002354544244287 48-49 21.90554957715195 27.06478135489945 28.936047789635378 22.093621278313226 50-51 22.02505747930719 27.404013603914702 28.73479729112283 21.836131625655277 52-53 22.1549952944052 27.27067145338641 28.623647467909514 21.950685784298877 54-55 21.980631431214846 27.291219818845196 28.7108644847614 22.017284265178557 56-57 21.98614506757307 27.574363781753497 28.53727740973729 21.902213740936148 58-59 22.083525371780343 27.256897594747816 28.608152312630065 22.051424720841776 60-61 21.98589667900146 27.258068641590967 28.840816424538257 21.91521825486932 62-63 21.875456789298497 27.336881846619026 28.984037954753322 21.803623409329152 64-65 21.77267348480222 27.29305611886168 28.83139077542068 22.102879620915424 66-67 21.690624329543017 27.393795698739503 28.66207193197579 22.25350803974169 68-69 21.830970475324413 27.313952990627566 28.750617294262216 22.104459239785804 70-71 21.81784679437021 27.261960006034226 28.64382883054529 22.27636436905027 72-73 21.822704327989683 27.291078317246708 28.652012089764938 22.234205264998668 74-75 21.774693678117718 27.612923567783376 28.43265303565612 22.17972971844279 76-77 21.892456186424177 27.373649776461612 28.49549242402488 22.23840161308933 78-79 22.033899190061863 27.37890610314911 28.45100897657069 22.136185730218337 80-81 22.016617635158596 27.530404320689556 28.34739889372388 22.10557915042797 82-83 21.95474025529933 27.619334505191134 28.33972947730513 22.0861957622044 84-85 21.96038941344196 27.546318410063904 28.311248857407005 22.182043319087136 86-87 21.967233482968105 27.585663252334964 28.29397046179354 22.153132802903393 88-89 21.9920790466455 27.50098411528297 28.13854463706768 22.36839220100385 90-91 21.952087942838823 27.422046061816012 28.26206807258542 22.36379792275974 92-93 21.958723113458973 27.20303753113412 28.39327320291328 22.444966152493627 94-95 21.905809498402768 27.293590554116133 28.35649785617444 22.44410209130666 96-97 21.8914213978301 27.18980245386284 28.333685674838378 22.585090473468686 98-99 21.827600103569818 27.203103335950416 28.216928653759414 22.752367906720345 100-101 22.04619149331546 26.569921973687784 28.776404609336016 22.607481923660742 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1362.0 1 796.5 2 251.0 3 264.5 4 219.5 5 198.0 6 310.5 7 629.5 8 1088.0 9 1318.0 10 1139.5 11 960.5 12 977.5 13 1038.0 14 1097.0 15 1189.0 16 1313.5 17 1394.0 18 1497.0 19 1628.0 20 1806.0 21 2173.5 22 2641.0 23 3164.5 24 3968.0 25 5024.5 26 6268.0 27 7743.0 28 9032.0 29 10448.5 30 12196.0 31 14216.5 32 16552.0 33 18172.0 34 19698.0 35 21394.5 36 23535.0 37 25847.5 38 27157.0 39 28281.0 40 29539.0 41 31056.5 42 32393.0 43 33832.5 44 35111.5 45 35812.5 46 36274.0 47 37233.0 48 38055.5 49 37131.0 50 35724.5 51 34117.5 52 32395.5 53 31434.0 54 30485.0 55 27911.5 56 24715.0 57 21919.0 58 19743.0 59 17309.5 60 13969.0 61 11347.5 62 9057.5 63 6700.0 64 4972.5 65 3780.0 66 2745.5 67 1991.5 68 1467.0 69 1059.0 70 796.0 71 607.5 72 457.5 73 351.0 74 252.5 75 189.0 76 139.0 77 83.0 78 52.5 79 39.5 80 24.0 81 17.5 82 12.5 83 9.0 84 6.5 85 3.0 86 2.5 87 5.0 88 4.5 89 3.0 90 2.5 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31073796619812827 2 0.29260637460910266 3 0.279268192290739 4 0.266242623620462 5 0.2629080780408711 6 0.21476557623552728 7 0.20642921228655 8 0.22028841735172475 9 0.2214346673947091 10-11 0.2214346673947091 12-13 0.21836063318852375 14-15 0.21445296258744065 16-17 0.21445296258744065 18-19 0.2148176785102084 20-21 0.2012710870931203 22-23 0.19251790494669416 24-25 0.19397676863776517 26-27 0.20549137134229004 28-29 0.22278932653641792 30-31 0.21601603082787388 32-33 0.22034051962640586 34-35 0.21382773529126736 36-37 0.19679029147054503 38-39 0.19939540520460042 40-41 0.19288262086946192 42-43 0.20611659863846335 44-45 0.20184421211461248 46-47 0.20095847344503365 48-49 0.21231676932551521 50-51 0.22221620151492574 52-53 0.2375863725458526 54-55 0.2101805760635898 56-57 0.1797528476498227 58-59 0.17990915447386602 60-61 0.18668245018241006 62-63 0.19580034825160395 64-65 0.18386892734963023 66-67 0.18918335936710326 68-69 0.19194477992520198 70-71 0.1856404046887879 72-73 0.18923546164178437 74-75 0.19121534807966648 76-77 0.20689813275868 78-79 0.21330671254445624 80-81 0.20147949619184474 82-83 0.19929120065523823 84-85 0.19293472314414303 86-87 0.19533142777947402 88-89 0.20194841666397473 90-91 0.20194841666397473 92-93 0.1979365415135294 94-95 0.1980407460628916 96-97 0.18480676829389017 98-99 0.1920489844745642 100-101 0.1843378478217602 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 959651.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.95180685631689 #Duplication Level Percentage of deduplicated Percentage of total 1 86.20237583287832 62.024166964829 2 8.707851565764999 12.530913079867997 3 2.1822663772130797 4.710540266468096 4 0.9174072080123368 2.640364249579864 5 0.5069303777561102 1.8237278314953698 6 0.31893428359174913 1.3768738783710799 7 0.22938931592790102 1.1553483028182892 8 0.15786930893698412 0.9087185620139283 9 0.11514294915312467 0.7456268914499109 >10 0.6190211716925559 7.745712919217289 >50 0.028449428863309464 1.3626944258075333 >100 0.013491745317943126 1.7587372479728807 >500 2.901449638479661E-4 0.1459264162498621 >1k 5.802899276959322E-4 1.070648963858967 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3825 0.39858240131047645 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3131 0.32626444405309846 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1942 0.2023652348614236 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1359 0.1416139825832516 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0420454936221606E-4 0.0 10-11 0.0 0.0 0.0 1.0420454936221606E-4 0.0 12-13 0.0 0.0 0.0 1.0420454936221606E-4 1.0420454936221606E-4 14-15 0.0 0.0 0.0 1.0420454936221606E-4 1.0420454936221606E-4 16-17 0.0 0.0 0.0 1.5630682404332407E-4 1.0420454936221606E-4 18-19 0.0 0.0 0.0 2.0840909872443212E-4 1.0420454936221606E-4 20-21 0.0 0.0 0.0 2.0840909872443212E-4 1.0420454936221606E-4 22-23 1.0420454936221606E-4 0.0 0.0 2.0840909872443212E-4 1.0420454936221606E-4 24-25 2.0840909872443212E-4 0.0 0.0 4.1681819744886423E-4 2.0840909872443212E-4 26-27 2.0840909872443212E-4 0.0 0.0 9.378409442599445E-4 2.0840909872443212E-4 28-29 2.6051137340554016E-4 0.0 0.0 0.001823579613838781 2.0840909872443212E-4 30-31 3.1261364808664815E-4 0.0 0.0 0.003855568326401994 2.0840909872443212E-4 32-33 3.647159227677562E-4 0.0 0.0 0.007450625279398448 2.0840909872443212E-4 34-35 4.1681819744886423E-4 0.0 0.0 0.010733068584308253 2.0840909872443212E-4 36-37 4.1681819744886423E-4 0.0 0.0 0.01505755738284022 2.0840909872443212E-4 38-39 4.1681819744886423E-4 0.0 0.0 0.018340000687750026 3.1261364808664815E-4 40-41 4.1681819744886423E-4 0.0 0.0 0.020840909872443213 3.1261364808664815E-4 42-43 4.1681819744886423E-4 0.0 0.0 0.02427966000139634 3.1261364808664815E-4 44-45 4.1681819744886423E-4 0.0 0.0 0.028552046525247197 3.1261364808664815E-4 46-47 4.1681819744886423E-4 0.0 0.0 0.034648012662936836 3.1261364808664815E-4 48-49 4.1681819744886423E-4 0.0 0.0 0.041265001547437556 3.1261364808664815E-4 50-51 4.1681819744886423E-4 0.0 0.0 0.04631892219150503 3.1261364808664815E-4 52-53 4.1681819744886423E-4 0.0 0.0 0.05585363845814781 3.1261364808664815E-4 54-55 4.1681819744886423E-4 0.0 0.0 0.07414153687121672 3.1261364808664815E-4 56-57 4.1681819744886423E-4 0.0 0.0 0.10847693588606691 3.1261364808664815E-4 58-59 4.1681819744886423E-4 0.0 0.0 0.1466679032273191 3.1261364808664815E-4 60-61 4.1681819744886423E-4 0.0 0.0 0.17855449533215723 4.1681819744886423E-4 62-63 4.1681819744886423E-4 0.0 0.0 0.21773540589235046 4.1681819744886423E-4 64-65 4.1681819744886423E-4 0.0 0.0 0.2704108055949507 4.1681819744886423E-4 66-67 4.1681819744886423E-4 0.0 0.0 0.34601120615723846 4.1681819744886423E-4 68-69 4.1681819744886423E-4 0.0 0.0 0.43281359577596434 4.1681819744886423E-4 70-71 4.1681819744886423E-4 0.0 0.0 0.5100291668533665 4.1681819744886423E-4 72-73 4.689204721299723E-4 0.0 0.0 0.5984988292618879 4.1681819744886423E-4 74-75 5.210227468110803E-4 0.0 0.0 0.6933770714561855 4.1681819744886423E-4 76-77 5.210227468110803E-4 0.0 0.0 0.8089920189735644 4.1681819744886423E-4 78-79 5.210227468110803E-4 0.0 0.0 0.9148117388508947 4.689204721299723E-4 80-81 5.210227468110803E-4 0.0 0.0 1.0211524814750361 5.210227468110803E-4 82-83 5.210227468110803E-4 0.0 0.0 1.1148323713516683 5.210227468110803E-4 84-85 5.210227468110803E-4 0.0 0.0 1.2702013544507325 5.210227468110803E-4 86-87 5.210227468110803E-4 0.0 0.0 1.4492247702550198 5.210227468110803E-4 88-89 5.210227468110803E-4 0.0 0.0 1.6805067675644583 5.210227468110803E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3945 0.0 39.045593 1 GGTATCA 2370 0.0 37.912937 1 ATCAACG 4785 0.0 31.681173 3 CGAACGG 30 0.0039513325 31.667112 74-75 TCAACGC 4785 0.0 31.580208 4 CAACGCA 4835 0.0 31.155348 5 TATCAAC 4910 0.0 30.876238 2 AACGCAG 5005 0.0 30.184183 6 TACGGTG 70 0.008384967 27.146784 7 CGCAGAG 5570 0.0 27.03712 8 ACGCAGA 5600 0.0 26.977114 7 GCAGAGT 5950 0.0 24.911163 9 TAGCCCT 555 0.0 23.97369 4 GTACATG 3050 0.0 23.381128 1 GGACCGA 185 2.537181E-7 23.111448 6 TACATGG 3050 0.0 22.749308 2 GAGTACT 3500 0.0 22.598515 12-13 ACATGGG 3085 0.0 22.027914 3 AGTACTT 3640 0.0 21.990355 12-13 TCTAGAC 420 0.0 21.497938 3 >>END_MODULE