FastQCFastQC Report
Thu 26 May 2016
SRR937841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937841_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959651
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42950.44755853951071795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38210.3981655831130275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24740.2578020551221225No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11420.11900159537165074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28100.045.6642341
GTATCAA44250.034.79771
ATCAACG52450.028.8908443
TCAACGC53350.028.4034654
TATCAAC54200.028.1333082
CAACGCA54600.027.7532025
AACGCAG55500.027.2147256
ACGCAGA62850.023.9565227
CGCAGAG62950.023.9184658
GAGTACT41950.023.83361412-13
GCAGAGT66850.022.238879
GTACATG30600.022.2092231
CGGACGG753.0352536E-522.16602930-31
GTACTTT45600.021.8217214-15
TACATGG31350.021.819262
ACATGGG30200.021.2344913
GTACTAC2257.10952E-821.1220591
CAGAGTA64600.021.10178410-11
CGCGCTT958.259896E-619.99890362-63
AGAGTAC56600.019.88848310-11
Produced by FastQC (version 0.11.5)