FastQCFastQC Report
Thu 26 May 2016
SRR937840_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937840_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867080
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35030.4039996309452415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29270.3375697744152789No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.2040180836831665No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12150.14012547861788993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20300.040.7588421
GTATCAA34300.037.1543161
ATCAACG41450.030.3905933
TATCAAC41700.030.2083912
TCAACGC42300.029.8940124
CAACGCA42700.029.5009355
AACGCAG43800.028.7434146
ACGCAGA50700.024.6427547
CGCAGAG50600.024.5036898
GTACATG29150.022.3486161
GCAGAGT54800.022.2802039
TACATGG27800.022.0578272
TGGTATC11950.021.878252
GAGTACT32700.021.71498712-13
GTGGTAT12850.021.0930961
ACATGGG28050.020.6749653
AGAGTAC46800.020.60292410-11
AGTACTT33900.019.82544112-13
GAGATCG1200.0047420419.8064465
GTACTTT35550.019.77314214-15
Produced by FastQC (version 0.11.5)