##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937840_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 867080 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.585845596715412 33.0 31.0 34.0 30.0 34.0 2 31.686068182866634 33.0 31.0 34.0 30.0 34.0 3 31.72837223785579 33.0 31.0 34.0 30.0 34.0 4 35.32336693269364 37.0 35.0 37.0 33.0 37.0 5 35.32079046916086 37.0 35.0 37.0 33.0 37.0 6 35.374190386123544 37.0 35.0 37.0 33.0 37.0 7 35.295615168150576 37.0 35.0 37.0 33.0 37.0 8 35.2956393873691 37.0 35.0 37.0 33.0 37.0 9 36.84037689717212 39.0 37.0 39.0 33.0 39.0 10-11 36.75186660977073 39.0 37.0 39.0 33.0 39.0 12-13 36.638159108732765 39.0 37.0 39.0 32.0 39.0 14-15 37.74146330211745 40.0 38.0 41.0 32.5 41.0 16-17 37.628567144900124 40.0 37.5 41.0 32.0 41.0 18-19 37.53981408866541 40.0 37.0 41.0 32.0 41.0 20-21 37.543641301840665 40.0 37.5 41.0 32.0 41.0 22-23 37.53615641001984 40.0 37.0 41.0 32.0 41.0 24-25 37.45899397979425 40.0 37.0 41.0 32.0 41.0 26-27 37.342309244821706 40.0 37.0 41.0 32.0 41.0 28-29 37.17516838123356 40.0 37.0 41.0 31.0 41.0 30-31 36.91530596946072 39.0 36.5 41.0 30.5 41.0 32-33 36.753062001199424 39.0 36.0 41.0 30.0 41.0 34-35 36.703410296627766 39.0 36.0 41.0 30.0 41.0 36-37 36.51856172440836 39.0 36.0 41.0 30.0 41.0 38-39 36.3286818978641 39.0 35.5 41.0 30.0 41.0 40-41 36.068247451215576 39.0 35.0 41.0 29.5 41.0 42-43 35.78234303639803 39.0 35.0 40.5 27.5 41.0 44-45 35.51656075563962 39.0 35.0 40.0 27.0 41.0 46-47 35.141529039996314 38.5 35.0 40.0 25.0 41.0 48-49 34.76106068644185 38.0 34.0 40.0 24.0 41.0 50-51 33.88980313235226 37.5 33.5 39.5 23.0 40.5 52-53 34.00291553259215 38.0 33.5 39.5 23.0 40.5 54-55 34.24212933062694 38.0 34.0 40.0 22.5 41.0 56-57 33.9659835309314 38.0 33.5 40.0 21.0 41.0 58-59 33.65654322553859 38.0 33.0 40.0 19.5 41.0 60-61 33.27985018683397 37.5 33.0 40.0 18.0 41.0 62-63 32.87986748627577 37.0 32.0 40.0 14.5 41.0 64-65 32.41640794390368 36.0 31.5 39.0 10.0 41.0 66-67 31.88571527425382 36.0 31.0 39.0 8.0 40.0 68-69 31.375008649720904 35.0 30.5 38.5 6.0 40.0 70-71 30.87667343267057 35.0 30.0 38.0 2.0 40.0 72-73 30.374097545785858 35.0 29.5 37.5 2.0 39.5 74-75 29.784703256908244 34.0 29.0 37.0 2.0 39.0 76-77 29.195642270609405 34.0 28.5 36.0 2.0 39.0 78-79 28.627218941735478 34.0 27.5 36.0 2.0 38.0 80-81 28.03238340176224 34.0 26.0 35.0 2.0 37.0 82-83 27.427631245098492 33.0 26.0 35.0 2.0 37.0 84-85 26.83427423075149 33.0 25.0 35.0 2.0 36.0 86-87 26.190322692254462 32.0 23.5 35.0 2.0 36.0 88-89 25.57962875397887 32.0 20.5 34.5 2.0 35.0 90-91 24.939933454813858 31.5 18.5 34.0 2.0 35.0 92-93 24.200806153988097 31.0 11.0 34.0 2.0 35.0 94-95 23.368318955575035 31.0 2.0 34.0 2.0 35.0 96-97 22.420699358767358 30.0 2.0 34.0 2.0 35.0 98-99 21.173582022420078 29.5 2.0 34.0 2.0 35.0 100-101 18.04490358444434 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10115824056178013 1101 2 -0.01381110491851345 1101 3 0.01901212446006184 1101 4 0.02022430054127966 1101 5 -0.024027807961431336 1101 6 0.1360059973365395 1101 7 0.002591260549664298 1101 8 0.10234781664900083 1101 9 0.34677038755216216 1101 10-11 0.15162679392741296 1101 12-13 0.3415453486786362 1101 14-15 0.42539840559192754 1101 16-17 0.5556782845524282 1101 18-19 0.4121718464194615 1101 20-21 0.2664991400432868 1101 22-23 0.2961350083663774 1101 24-25 0.3026937661515703 1101 26-27 0.36505567450941356 1101 28-29 0.17885939985976762 1101 30-31 0.5155296823928097 1101 32-33 0.35434197362816633 1101 34-35 0.3844808520153933 1101 36-37 0.5099149517914583 1101 38-39 0.6088640053436905 1101 40-41 0.6262299533619142 1101 42-43 0.5335422244112067 1101 44-45 0.5482688216010061 1101 46-47 0.49358359008139985 1101 48-49 0.8243599904629519 1101 50-51 0.4239255871983332 1101 52-53 0.6748986068891796 1101 54-55 0.8564793038269158 1101 56-57 1.2632832465744386 1101 58-59 1.1228708163564605 1101 60-61 1.032234190426749 1101 62-63 1.0372623562393102 1101 64-65 1.0643646966738416 1101 66-67 1.1597090473084783 1101 68-69 1.33945663022239 1101 70-71 1.2109925125370467 1101 72-73 1.2968031551226709 1101 74-75 1.3806235197237982 1101 76-77 1.3645578778404506 1101 78-79 1.4786493215112522 1101 80-81 1.7339563896504941 1101 82-83 1.7582036655756603 1101 84-85 1.7815618086691387 1101 86-87 2.2146186911662937 1101 88-89 2.08325463595229 1101 90-91 2.0447342041531584 1101 92-93 2.546509657793962 1101 94-95 2.5497175083583237 1101 96-97 2.8418617660504317 1101 98-99 3.1005511387612295 1101 100-101 3.8171850437084913 1102 1 0.2830120318453311 1102 2 0.2068523181631683 1102 3 0.20833578201445135 1102 4 0.14353894202892548 1102 5 0.21214915983637184 1102 6 0.3077304575407709 1102 7 0.280027728890353 1102 8 0.30566287045677853 1102 9 0.5861222203151186 1102 10-11 0.5016762723424506 1102 12-13 0.4234321255722051 1102 14-15 0.5921148942737418 1102 16-17 0.6730366182293608 1102 18-19 0.646553503671484 1102 20-21 0.6450846071332705 1102 22-23 0.601954563335525 1102 24-25 0.36493520707296767 1102 26-27 0.5718187553653848 1102 28-29 0.706938400395785 1102 30-31 0.6429950131422757 1102 32-33 0.7394936387142721 1102 34-35 0.6360992085073676 1102 36-37 1.1446336807459474 1102 38-39 1.0731708264929694 1102 40-41 0.9137957527819225 1102 42-43 0.9564795929841665 1102 44-45 1.1589132092368999 1102 46-47 1.1938766809705044 1102 48-49 1.2003066087507221 1102 50-51 0.9871926744315331 1102 52-53 1.1325000720000205 1102 54-55 1.367244295099475 1102 56-57 1.448643476923948 1102 58-59 1.4403023368556447 1102 60-61 1.4556354191407266 1102 62-63 1.687815896007578 1102 64-65 1.5874271356848269 1102 66-67 1.759913145770522 1102 68-69 1.6613944660914832 1102 70-71 1.4874480025795123 1102 72-73 1.62864182800163 1102 74-75 1.718219212352892 1102 76-77 1.8238326754599896 1102 78-79 2.204258868096556 1102 80-81 2.4690269651119507 1102 82-83 2.6489542943638504 1102 84-85 2.7708603834159042 1102 86-87 2.604683837746098 1102 88-89 2.8153324545039453 1102 90-91 3.0314282901081597 1102 92-93 3.054491488220439 1102 94-95 3.2807782706007984 1102 96-97 3.404955335230877 1102 98-99 3.48948412299686 1102 100-101 4.18937267685353 1103 1 0.15735135302489667 1103 2 0.09743376499104173 1103 3 0.2698218858087351 1103 4 0.2744677383506442 1103 5 0.09156862812749722 1103 6 0.12702413406846347 1103 7 0.21385967841535347 1103 8 0.2670201156023211 1103 9 0.2744685508115836 1103 10-11 0.21364442125457117 1103 12-13 0.3389190307139387 1103 14-15 0.2712902483876931 1103 16-17 0.42480811548772124 1103 18-19 0.43286641501129 1103 20-21 0.4619519816568456 1103 22-23 0.2644209574451253 1103 24-25 0.3267258970974254 1103 26-27 0.37756372406584404 1103 28-29 0.3444399771210982 1103 30-31 0.23586166051723723 1103 32-33 0.43199015755211434 1103 34-35 0.3747084502199769 1103 36-37 0.4521792079163305 1103 38-39 0.31268838564798784 1103 40-41 0.4410180698137651 1103 42-43 0.2144361817173177 1103 44-45 0.14981457672148935 1103 46-47 -0.25047295384754875 1103 48-49 -0.1258096509353166 1103 50-51 -0.03033370943862934 1103 52-53 -0.07731321899050414 1103 54-55 -0.24286183397187955 1103 56-57 -0.1767667808180846 1103 58-59 -0.3510678436206689 1103 60-61 0.13978827864027465 1103 62-63 0.2225186925617919 1103 64-65 0.22923162669316355 1103 66-67 0.6000894669426735 1103 68-69 0.6979874780449187 1103 70-71 0.70871821248803 1103 72-73 0.44966062751389657 1103 74-75 0.7051107509647281 1103 76-77 0.933230205628 1103 78-79 1.2542144724584574 1103 80-81 1.3867658049883858 1103 82-83 1.300437405471662 1103 84-85 1.2286882371650485 1103 86-87 1.178172221918004 1103 88-89 1.394001154218774 1103 90-91 1.6516551406890159 1103 92-93 1.794572958544613 1103 94-95 2.0692478948331505 1103 96-97 2.1359072568571165 1103 98-99 2.7078661431344244 1103 100-101 3.309104250190426 1104 1 0.2938697951460938 1104 2 0.22965362109476217 1104 3 0.3245138493325861 1104 4 0.36069420804413 1104 5 0.36198629338685606 1104 6 0.2990442469001664 1104 7 0.4059777831791678 1104 8 0.39578230585308916 1104 9 0.41999844181349033 1104 10-11 0.4240432647420178 1104 12-13 0.453290974649299 1104 14-15 0.5866860126233533 1104 16-17 0.5568585509112296 1104 18-19 0.6042082267985194 1104 20-21 0.38232673525487115 1104 22-23 0.3869708499804787 1104 24-25 0.3285617000154204 1104 26-27 0.4496689182318292 1104 28-29 0.4555811799831915 1104 30-31 0.5621046765516056 1104 32-33 0.39150232492490744 1104 34-35 0.3923424224053065 1104 36-37 0.43290729638113135 1104 38-39 0.7202935192182593 1104 40-41 0.8307338635311083 1104 42-43 0.9195631977615832 1104 44-45 1.0818230898015102 1104 46-47 1.1015856929140426 1104 48-49 0.9608929279689562 1104 50-51 0.7977247048332714 1104 52-53 0.9099159243344417 1104 54-55 0.8270705708866615 1104 56-57 0.9844740958164593 1104 58-59 1.2736356701889733 1104 60-61 1.2080784158833993 1104 62-63 1.4663175246720783 1104 64-65 1.414788699202738 1104 66-67 1.6220195518508724 1104 68-69 1.5951621099568492 1104 70-71 1.7952655921560705 1104 72-73 1.7616494284359412 1104 74-75 1.6471008627329127 1104 76-77 1.6723668774143867 1104 78-79 1.8155509419293416 1104 80-81 2.0178868999653723 1104 82-83 1.9953169436580929 1104 84-85 2.411468418160748 1104 86-87 2.534108376291158 1104 88-89 2.7181554729621418 1104 90-91 2.5661731326705954 1104 92-93 2.9426565262226134 1104 94-95 3.3024937972023167 1104 96-97 3.4261279736673593 1104 98-99 3.639321256547344 1104 100-101 3.900556173053314 1105 1 0.06049645952691307 1105 2 0.13279856271047663 1105 3 0.07247278031911719 1105 4 0.054760535643801234 1105 5 0.06708182265519724 1105 6 0.14106188600334946 1105 7 0.15568919525986047 1105 8 0.20447232623520506 1105 9 0.3681783378075494 1105 10-11 0.17303844541900304 1105 12-13 0.29391043861866706 1105 14-15 0.3981628589193349 1105 16-17 0.4212660408630313 1105 18-19 0.4296287270175796 1105 20-21 0.33400968856648916 1105 22-23 0.4138028594286354 1105 24-25 0.3875612618951507 1105 26-27 0.38991502753331986 1105 28-29 0.3116967675405746 1105 30-31 0.4574091701417231 1105 32-33 0.4709716279327054 1105 34-35 0.46465703085912224 1105 36-37 0.47727745086614703 1105 38-39 0.4885812308970259 1105 40-41 0.6879961832827135 1105 42-43 0.5014154940832753 1105 44-45 0.6356642616779951 1105 46-47 0.5258861291294465 1105 48-49 0.5625863580697228 1105 50-51 0.4931053932535647 1105 52-53 0.5196002872504195 1105 54-55 0.7666614046485947 1105 56-57 0.9197770655099617 1105 58-59 0.7157866502449721 1105 60-61 0.7708343828910316 1105 62-63 0.898205061102729 1105 64-65 1.1041899983245926 1105 66-67 1.0767236301791563 1105 68-69 1.2831404706250815 1105 70-71 1.3252330188661645 1105 72-73 1.2676707248909764 1105 74-75 1.2504343865533194 1105 76-77 1.4278138553369324 1105 78-79 1.5918238718110516 1105 80-81 1.5462370914048833 1105 82-83 1.5280959596018633 1105 84-85 1.7824667609231923 1105 86-87 2.020174246721851 1105 88-89 1.9939178974816407 1105 90-91 1.9294129368498787 1105 92-93 2.2475349002249345 1105 94-95 2.5923446458222372 1105 96-97 2.380508223967375 1105 98-99 2.741607101452768 1105 100-101 3.108975701788289 1106 1 -0.27182972630804514 1106 2 -0.420515277445471 1106 3 -0.30631214495703674 1106 4 -0.21086649489551235 1106 5 -0.10652116629866981 1106 6 -0.2201629353091903 1106 7 -0.3360749372937448 1106 8 -0.26659654965823165 1106 9 -0.09247793249784309 1106 10-11 -0.45715973521777187 1106 12-13 -0.4308702631357164 1106 14-15 -0.44667144907286627 1106 16-17 -0.3765572209953092 1106 18-19 -0.17080986947365062 1106 20-21 -0.10117549661868708 1106 22-23 -0.09944437903789805 1106 24-25 -0.3551531630561229 1106 26-27 -0.2063812687629749 1106 28-29 0.03586830750159464 1106 30-31 0.17526167176615104 1106 32-33 0.39385986704900944 1106 34-35 0.18267197562845894 1106 36-37 -0.0777745309402178 1106 38-39 -0.2847131160815337 1106 40-41 -0.018607130557441565 1106 42-43 0.18538235568171757 1106 44-45 0.37051643841613924 1106 46-47 0.18759827987668132 1106 48-49 0.27740929504179235 1106 50-51 0.32291371034258276 1106 52-53 0.16436961798466143 1106 54-55 0.47461299659141076 1106 56-57 0.5345756360102882 1106 58-59 -0.025287098942804676 1106 60-61 0.3272021541710828 1106 62-63 0.24199161081033083 1106 64-65 0.6218150795461739 1106 66-67 0.8689507256957221 1106 68-69 0.9611944017491858 1106 70-71 0.9744614139278909 1106 72-73 1.035287683955307 1106 74-75 0.8116185970796366 1106 76-77 1.2059002750900198 1106 78-79 1.5374866398422427 1106 80-81 1.5071045377986465 1106 82-83 1.2000988835784732 1106 84-85 1.2692844997146153 1106 86-87 1.709989061536664 1106 88-89 1.5433655907889836 1106 90-91 1.9368853215152484 1106 92-93 1.955567713740205 1106 94-95 2.0985337296428987 1106 96-97 2.3655959432656246 1106 98-99 2.3374729234150813 1106 100-101 2.4841056563042088 1107 1 0.16983202709997158 1107 2 0.38997140315698786 1107 3 0.26698027433152305 1107 4 0.1846240269818935 1107 5 0.18953170036442657 1107 6 0.18556726425844516 1107 7 0.3666572811765292 1107 8 0.2609511982826618 1107 9 0.46441313609124535 1107 10-11 0.4279660442037212 1107 12-13 0.5256239481522726 1107 14-15 0.6218551093234481 1107 16-17 0.5338294077945989 1107 18-19 0.8085879436795906 1107 20-21 0.7807052987932153 1107 22-23 0.5735146086521539 1107 24-25 0.5942123565879811 1107 26-27 0.5324299013072391 1107 28-29 0.6503829668787731 1107 30-31 0.5718978762291798 1107 32-33 0.2952351328116336 1107 34-35 0.4800648219238539 1107 36-37 0.7536850642072679 1107 38-39 0.7103557588715717 1107 40-41 0.8818156330165436 1107 42-43 0.6812887179050122 1107 44-45 0.7625419962943241 1107 46-47 0.8524448075427955 1107 48-49 0.961945706755003 1107 50-51 0.7584450569327785 1107 52-53 0.5577787048332041 1107 54-55 0.7764311256225156 1107 56-57 0.7319926019390692 1107 58-59 0.8058899838321949 1107 60-61 1.1395558730612194 1107 62-63 1.143151363182099 1107 64-65 0.9821869685601214 1107 66-67 1.0326794870185516 1107 68-69 1.4293274527934763 1107 70-71 1.5559131925275267 1107 72-73 1.536242419443326 1107 74-75 1.3137793142367329 1107 76-77 1.2855240981412415 1107 78-79 1.4424217848280385 1107 80-81 1.9225719226943951 1107 82-83 2.0558132267204563 1107 84-85 2.109508678307101 1107 86-87 2.0536201383442965 1107 88-89 2.215758437084382 1107 90-91 2.099701780038412 1107 92-93 2.1899558343741035 1107 94-95 2.5175788601569167 1107 96-97 2.6910903957499457 1107 98-99 3.1526640491766393 1107 100-101 3.451113124457361 1108 1 0.21701167920002717 1108 2 0.25825965132945683 1108 3 0.1649604096488524 1108 4 0.2696653189696363 1108 5 0.2003401822293469 1108 6 0.3167553932758054 1108 7 0.3993239231051149 1108 8 0.21405397265895232 1108 9 0.20073194404536565 1108 10-11 0.2829874632627636 1108 12-13 0.2277835830181303 1108 14-15 0.26486701246559363 1108 16-17 0.30672862895720243 1108 18-19 0.5510562273924506 1108 20-21 0.3689099734667778 1108 22-23 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-2.160556838658195 2316 96-97 -2.2420288880434214 2316 98-99 -2.4285683371437194 2316 100-101 -2.652202885706087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4695.0 3 1142.0 4 1014.0 5 1128.0 6 1286.0 7 1460.0 8 1811.0 9 2194.0 10 2750.0 11 3571.0 12 4205.0 13 5189.0 14 5857.0 15 6924.0 16 8244.0 17 10169.0 18 9103.0 19 7207.0 20 7404.0 21 7769.0 22 8636.0 23 9572.0 24 10724.0 25 11878.0 26 13496.0 27 15512.0 28 18284.0 29 21694.0 30 25595.0 31 31051.0 32 38395.0 33 49317.0 34 64350.0 35 87986.0 36 120089.0 37 147335.0 38 92535.0 39 7509.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.66073639652191 18.63163278767442 14.789262099116593 22.91836871668707 2 15.155395167539934 19.615531420243588 41.589576321177 23.639497091039477 3 19.1790107319917 24.10395156607497 29.949058319202145 26.767979382731188 4 12.526993343473308 16.53999410107973 37.55784700976792 33.37516554567904 5 13.556544107781496 36.87812585303112 34.81303871084153 14.752291328345848 6 31.737329026367643 36.00018491444818 17.845284392642714 14.417201666541466 7 28.112335165755848 32.54828454513202 21.051634099468533 18.2877461896436 8 25.77037448816022 35.40457734718195 19.90578565311507 18.919262511542758 9 25.632458233890215 17.28501505371373 20.75956243101585 36.3229642813802 10-11 24.495102863274855 26.644133693075013 27.755488150541375 21.105275293108757 12-13 25.42266609032133 24.017362018071662 28.325620476594448 22.23435141501256 14-15 22.375134784050815 25.52801254632931 26.660545284551812 25.436307385068062 16-17 21.597362863407994 28.797023109613935 27.470769906365472 22.134844120612595 18-19 21.542401064952145 27.16384848005121 30.010018592751713 21.283731862244927 20-21 22.00720937877896 26.303254966919944 30.397139821389057 21.292395832912046 22-23 21.84269616176231 26.113202183241093 30.268375015456726 21.77572663953987 24-25 21.712021743627197 26.261589147600358 30.360417723552246 21.665971385220203 26-27 21.374856984363625 26.596170070149896 30.190283026499788 21.8386899189867 28-29 21.419346040071126 26.746560044565847 30.249995232517353 21.584098682845674 30-31 21.501931925487664 26.463333868855603 30.209524051692156 21.82521015396458 32-33 21.25478629406482 26.56706706070402 30.247298120386347 21.93084852484481 34-35 21.845215358148174 26.631966496440505 29.951636661825905 21.571181483585416 36-37 21.792803547263915 26.335337548232317 30.01671013690525 21.85514876759851 38-39 21.448338737223054 26.643294747326753 30.059820250373804 21.84854626507639 40-41 21.790638813158143 26.583716223750127 29.97302579621275 21.652619166878985 42-43 21.60084310854676 26.494651414598856 29.86436999707639 22.04013547977799 44-45 21.623818782193695 26.82259271350033 29.7912736496344 21.76231485467157 46-47 21.842816455092766 27.004838203606724 29.245215968325454 21.907129372975053 48-49 21.844151881529356 27.026722467052 29.045903560568497 22.083222090850146 50-51 21.890343722497953 27.357844573382035 28.91948411866034 21.832327585459677 52-53 21.994026427970372 27.251648280517465 28.810579510462997 21.943745781049167 54-55 21.859288206833867 27.22330631167344 28.94434085082114 21.97306463067156 56-57 21.844777695552985 27.520483504878896 28.76257851552864 21.872160284039474 58-59 21.912875724880436 27.19745627613613 28.849317283922094 22.04035071506134 60-61 21.80293803823066 27.205247313865417 29.11146555872581 21.880349089178107 62-63 21.712661353025922 27.266509621972084 29.226834078313622 21.793994946688372 64-65 21.662947260300484 27.187868823479455 29.05850973795083 22.090674178269232 66-67 21.704652065607714 27.38576650937946 28.76085489677738 22.14872652823544 68-69 21.663506742135137 27.266220464526235 28.98465328060245 22.08561951273617 70-71 21.722312244915642 27.13112599614684 28.900866239756912 22.24569551918061 72-73 21.75963532789869 27.20735814936997 28.853408979299427 22.179597543431917 74-75 21.64210494689561 27.628785804007762 28.592245406883293 22.136863842213337 76-77 21.775036473154312 27.380144159239627 28.616042409972696 22.22877695763336 78-79 21.863514952481044 27.30296056755815 28.671589679353215 22.161934800607586 80-81 21.82540691319108 27.470829125347517 28.587168281685845 22.116595679775553 82-83 21.778146984641587 27.41568931473486 28.69162895326388 22.114534747359677 84-85 21.86156751357943 27.4437052274522 28.54727397607664 22.14745328289174 86-87 21.89253501297804 27.422861107532576 28.49813489420284 22.18646898528655 88-89 21.91813825039215 27.40875121689117 28.305654174695015 22.36745635802166 90-91 21.822767931680147 27.2974382942683 28.463294311627884 22.416499462423676 92-93 21.813113807047948 27.051068746389372 28.658405545927213 22.47741190063547 94-95 21.76367281151249 27.02511279672326 28.70236107660934 22.508853315154912 96-97 21.78197686343092 26.987676018702917 28.610921898046804 22.619425219819362 98-99 21.70293258825555 27.03119782115591 28.374833749897448 22.891035840691092 100-101 22.09804331576935 26.246163052783224 29.104190518220374 22.551603113227056 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1134.0 1 677.5 2 242.0 3 265.5 4 227.0 5 207.0 6 303.0 7 627.0 8 1037.0 9 1242.0 10 1126.5 11 895.5 12 862.5 13 943.0 14 1038.5 15 1125.5 16 1211.5 17 1325.0 18 1403.5 19 1481.0 20 1708.5 21 2042.5 22 2479.5 23 3014.5 24 3655.0 25 4501.5 26 5735.5 27 7187.0 28 8401.0 29 9675.5 30 11221.5 31 13058.5 32 15072.5 33 16594.5 34 18103.5 35 19616.5 36 21577.5 37 23815.0 38 25027.5 39 25732.5 40 26668.5 41 28156.0 42 29424.0 43 30547.0 44 31727.5 45 32457.0 46 32808.5 47 33385.0 48 33896.5 49 33408.0 50 32206.5 51 30525.0 52 29016.0 53 28221.5 54 27279.0 55 24900.5 56 22019.0 57 19642.5 58 17510.5 59 15085.0 60 12266.0 61 9971.5 62 8068.5 63 6077.0 64 4386.0 65 3284.0 66 2399.5 67 1760.0 68 1316.5 69 963.0 70 729.5 71 533.5 72 389.0 73 293.5 74 211.0 75 157.0 76 113.5 77 71.0 78 50.5 79 34.5 80 21.5 81 16.5 82 13.5 83 10.0 84 6.5 85 3.0 86 2.5 87 2.5 88 2.5 89 2.5 90 2.0 91 2.0 92 2.0 93 1.5 94 2.0 95 1.5 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33745444480324766 2 0.28947732619827465 3 0.29547446602389627 4 0.2900539742584306 5 0.2901693038704618 6 0.209323245836601 7 0.20078885454629333 8 0.20978456428472572 9 0.21278313419753658 10-11 0.2109955252110532 12-13 0.21428241915394194 14-15 0.2084006089403515 16-17 0.20153849702449603 18-19 0.1947917147206717 20-21 0.19369608340637542 22-23 0.20309544678691702 24-25 0.19871292152973197 26-27 0.20644000553582137 28-29 0.21261013977948978 30-31 0.21693500023065923 32-33 0.2132444526456613 34-35 0.21434008395995757 36-37 0.20032753609816856 38-39 0.1907551782995802 40-41 0.18723762513262907 42-43 0.19732896618535775 44-45 0.19750196060340452 46-47 0.20522904460949393 48-49 0.21370577109378605 50-51 0.2079969552982424 52-53 0.2232781288923744 54-55 0.20603635189371225 56-57 0.1806638372468515 58-59 0.16376804908428286 60-61 0.18112515569497625 62-63 0.17403238455505834 64-65 0.18158647414310097 66-67 0.18129815011302303 68-69 0.17910688748443052 70-71 0.17962587073857084 72-73 0.18971721179129952 74-75 0.19709830696129538 76-77 0.19807860866356045 78-79 0.19473404991465607 80-81 0.1916778151958297 82-83 0.18002952438067998 84-85 0.1758199935415417 86-87 0.18216312220325692 88-89 0.19115883194168934 90-91 0.1870069659085667 92-93 0.1822207870092725 94-95 0.18279743506942842 96-97 0.1906975134935646 98-99 0.1922544632559856 100-101 0.17103381464224757 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 867080.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.80290891330762 #Duplication Level Percentage of deduplicated Percentage of total 1 86.65064548907489 63.084190508204294 2 8.398039691800411 12.228034374649747 3 2.146810474784769 4.688821423496707 4 0.894857568967535 2.6059293633570935 5 0.500730868149463 1.8227331891983403 6 0.31049213919691704 1.356283855695071 7 0.21294322281588457 1.0852020238059708 8 0.14984343035427528 0.8727230089071885 9 0.11534504961582548 0.7557709626703701 >10 0.5799162762055251 7.334640585035402 >50 0.02718420082371267 1.3162171279147945 >100 0.012396914265419248 1.6643604654776298 >500 1.5893478909329977E-4 0.09580717598070636 >1k 6.357391563731991E-4 1.0892859356067268 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3503 0.4039996309452415 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2927 0.3375697744152789 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1769 0.2040180836831665 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1215 0.14012547861788993 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 1.1532961203118512E-4 9 0.0 0.0 0.0 0.0 1.1532961203118512E-4 10-11 0.0 0.0 0.0 0.0 1.1532961203118512E-4 12-13 0.0 0.0 0.0 0.0 2.3065922406237025E-4 14-15 0.0 0.0 0.0 0.0 2.3065922406237025E-4 16-17 0.0 0.0 0.0 0.0 2.3065922406237025E-4 18-19 0.0 0.0 0.0 0.0 2.3065922406237025E-4 20-21 0.0 0.0 0.0 0.0 2.3065922406237025E-4 22-23 0.0 0.0 0.0 5.766480601559256E-5 2.3065922406237025E-4 24-25 1.1532961203118512E-4 0.0 0.0 1.1532961203118512E-4 2.3065922406237025E-4 26-27 1.1532961203118512E-4 0.0 0.0 1.1532961203118512E-4 2.3065922406237025E-4 28-29 1.1532961203118512E-4 0.0 0.0 0.0010956313142962586 2.3065922406237025E-4 30-31 1.1532961203118512E-4 0.0 0.0 0.0034022235549199613 2.3065922406237025E-4 32-33 1.1532961203118512E-4 0.0 0.0 0.006573787885777552 2.3065922406237025E-4 34-35 1.1532961203118512E-4 0.0 0.0 0.010610324306869031 3.459888360935554E-4 36-37 1.1532961203118512E-4 0.0 0.0 0.014243207085851363 3.459888360935554E-4 38-39 1.1532961203118512E-4 0.0 0.0 0.017126447386630994 3.459888360935554E-4 40-41 1.1532961203118512E-4 0.0 0.0 0.019087050791161138 3.459888360935554E-4 42-43 1.1532961203118512E-4 0.0 0.0 0.021278313419753656 3.459888360935554E-4 44-45 1.1532961203118512E-4 0.0 0.0 0.02433454813858006 3.459888360935554E-4 46-47 1.1532961203118512E-4 0.0 0.0 0.028890067813811874 3.459888360935554E-4 48-49 1.1532961203118512E-4 0.0 0.0 0.0340799003552152 4.613184481247405E-4 50-51 1.1532961203118512E-4 0.0 0.0 0.03823176638833787 4.613184481247405E-4 52-53 1.729944180467777E-4 0.0 0.0 0.045439867140286944 5.189832541403331E-4 54-55 3.459888360935554E-4 0.0 0.0 0.06008672786824745 5.766480601559256E-4 56-57 3.459888360935554E-4 0.0 0.0 0.09174470637080777 6.343128661715181E-4 58-59 3.459888360935554E-4 0.0 0.0 0.12524795866586705 6.919776721871107E-4 60-61 3.459888360935554E-4 0.0 0.0 0.1526387415232735 6.919776721871107E-4 62-63 3.459888360935554E-4 0.0 0.0 0.18844858605895648 6.919776721871107E-4 64-65 4.613184481247405E-4 0.0 0.0 0.23452276606541494 6.919776721871107E-4 66-67 4.613184481247405E-4 1.1532961203118512E-4 0.0 0.30741108086912394 6.919776721871107E-4 68-69 4.613184481247405E-4 1.1532961203118512E-4 0.0 0.40203902754071136 6.919776721871107E-4 70-71 5.189832541403331E-4 1.1532961203118512E-4 0.0 0.48357706324675925 6.919776721871107E-4 72-73 5.766480601559256E-4 1.1532961203118512E-4 0.0 0.5742261383032707 8.073072842182959E-4 74-75 5.766480601559256E-4 1.1532961203118512E-4 0.0 0.6722563085297781 8.073072842182959E-4 76-77 5.766480601559256E-4 1.1532961203118512E-4 0.0 0.7894888591594778 8.073072842182959E-4 78-79 5.766480601559256E-4 1.1532961203118512E-4 0.0 0.9036075102643355 8.073072842182959E-4 80-81 5.766480601559256E-4 1.1532961203118512E-4 0.0 1.007404161092402 8.073072842182959E-4 82-83 5.766480601559256E-4 1.1532961203118512E-4 0.0 1.1014554597038335 8.073072842182959E-4 84-85 5.766480601559256E-4 1.1532961203118512E-4 0.0 1.2584190616782767 8.073072842182959E-4 86-87 5.766480601559256E-4 1.1532961203118512E-4 0.0 1.4386792452830188 8.073072842182959E-4 88-89 5.766480601559256E-4 1.1532961203118512E-4 0.0 1.6809290953545233 8.073072842182959E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2030 0.0 40.758842 1 GTATCAA 3430 0.0 37.154316 1 ATCAACG 4145 0.0 30.390593 3 TATCAAC 4170 0.0 30.208391 2 TCAACGC 4230 0.0 29.894012 4 CAACGCA 4270 0.0 29.500935 5 AACGCAG 4380 0.0 28.743414 6 ACGCAGA 5070 0.0 24.642754 7 CGCAGAG 5060 0.0 24.503689 8 GTACATG 2915 0.0 22.348616 1 GCAGAGT 5480 0.0 22.280203 9 TACATGG 2780 0.0 22.057827 2 TGGTATC 1195 0.0 21.87825 2 GAGTACT 3270 0.0 21.714987 12-13 GTGGTAT 1285 0.0 21.093096 1 ACATGGG 2805 0.0 20.674965 3 AGAGTAC 4680 0.0 20.602924 10-11 AGTACTT 3390 0.0 19.825441 12-13 GAGATCG 120 0.00474204 19.806446 5 GTACTTT 3555 0.0 19.773142 14-15 >>END_MODULE