FastQCFastQC Report
Thu 26 May 2016
SRR937840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937840_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867080
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39630.4570512524795867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34850.40192369792868016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21140.24380679983392536No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10360.1194814780643078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24050.046.4292831
GTATCAA38700.035.9745861
ATAACGC600.00394840331.6699733
ATCAACG49550.027.9948483
TATCAAC49850.027.9216732
TCAACGC50350.027.4556984
CAACGCA51450.026.8686945
AACGCAG52450.026.3518626
ACACCGT1301.0022697E-525.5751696
ACGACCC1401.7781842E-523.751118
ACGCAGA58650.023.4878857
CGCAGAG59700.023.074788
TAGGGTG2405.9153535E-921.7731085
GAGTACT41650.021.5556312-13
GTGGTAT14250.021.0070381
TGGTATC12900.020.9905642
GCAGAGT65350.020.8617259
GTACTTT43300.020.56729514-15
GGACCGA1856.552931E-620.5391336
TAAGGTG4400.020.5135065
Produced by FastQC (version 0.11.5)