##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937840_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 867080 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23944272731467 33.0 31.0 34.0 31.0 34.0 2 32.45624971167597 34.0 31.0 34.0 31.0 34.0 3 32.56920699358767 34.0 31.0 34.0 31.0 34.0 4 36.17735157078931 37.0 35.0 37.0 35.0 37.0 5 36.02362988420907 37.0 35.0 37.0 35.0 37.0 6 36.11488905291323 37.0 35.0 37.0 35.0 37.0 7 36.08015984684228 37.0 35.0 37.0 35.0 37.0 8 36.07222632283065 37.0 35.0 37.0 35.0 37.0 9 37.74334778797804 39.0 37.0 39.0 35.0 39.0 10-11 37.69421967984499 39.0 37.0 39.0 35.0 39.0 12-13 37.616248212391014 39.0 37.0 39.0 35.0 39.0 14-15 38.869184504313324 40.0 38.0 41.0 35.0 41.0 16-17 38.83345596715412 40.0 38.0 41.0 35.0 41.0 18-19 38.80546835355446 40.0 38.0 41.0 35.0 41.0 20-21 38.72030320155003 40.0 38.0 41.0 34.5 41.0 22-23 38.572864672233244 40.0 38.0 41.0 34.0 41.0 24-25 38.52291830050284 40.0 38.0 41.0 34.0 41.0 26-27 38.456159754578586 40.0 38.0 41.0 34.0 41.0 28-29 38.337561124694375 40.0 38.0 41.0 34.0 41.0 30-31 38.39656375420953 40.0 38.0 41.0 34.0 41.0 32-33 38.31795855053744 40.0 38.0 41.0 34.0 41.0 34-35 38.18608432901232 40.0 38.0 41.0 34.0 41.0 36-37 38.0276485445403 40.0 38.0 41.0 33.5 41.0 38-39 37.885206094016695 40.0 37.0 41.0 33.0 41.0 40-41 37.69419430733035 40.0 37.0 41.0 33.0 41.0 42-43 37.49468791806984 40.0 37.0 41.0 33.0 41.0 44-45 37.205070466392954 40.0 37.0 41.0 32.0 41.0 46-47 36.8596069566822 40.0 36.0 41.0 31.0 41.0 48-49 36.54027886700189 39.5 36.0 41.0 30.5 41.0 50-51 36.286267126447385 39.0 36.0 41.0 30.0 41.0 52-53 36.05773804031924 39.0 35.0 40.5 29.5 41.0 54-55 35.83973393458504 39.0 35.0 40.0 29.0 41.0 56-57 35.552874590579876 38.5 35.0 40.0 28.0 41.0 58-59 35.291996124925035 38.0 35.0 40.0 28.0 41.0 60-61 34.939742007657884 38.0 34.0 40.0 27.0 41.0 62-63 34.55840637542096 37.5 34.0 40.0 26.0 41.0 64-65 34.14703948885916 37.0 34.0 39.5 26.0 41.0 66-67 33.71615767864557 36.5 33.0 39.0 25.5 40.5 68-69 33.26590280020298 36.0 33.0 39.0 24.5 40.0 70-71 32.844853992711165 35.0 32.0 38.5 23.5 40.0 72-73 32.39474385293168 35.0 32.0 38.0 22.0 40.0 74-75 31.898334063754206 35.0 31.0 37.0 21.0 39.0 76-77 30.693139041380263 34.0 29.5 36.0 20.0 38.5 78-79 31.24167954514001 34.0 31.0 36.0 20.0 38.5 80-81 31.025289477326197 34.0 31.0 36.0 19.5 37.5 82-83 30.653613276744938 34.0 31.0 35.0 18.0 37.0 84-85 30.246284079900356 34.0 30.5 35.0 15.5 36.5 86-87 29.81319947409697 34.0 30.0 35.0 9.0 36.0 88-89 29.366093670710892 34.0 29.5 35.0 4.5 36.0 90-91 28.83874786640218 34.0 29.0 35.0 2.0 35.0 92-93 28.372648429210685 33.5 29.0 35.0 2.0 35.0 94-95 27.886991973059004 33.0 27.5 35.0 2.0 35.0 96-97 27.31798680629238 33.0 27.0 35.0 2.0 35.0 98-99 26.459116229183003 33.0 25.0 34.5 2.0 35.0 100-101 24.003160608017716 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25333960928630006 1101 2 0.1440918634551167 1101 3 0.08790472257050652 1101 4 0.010474069124242646 1101 5 -0.019314543331347522 1101 6 0.08763145871937184 1101 7 0.10769046636879409 1101 8 0.11126389593891872 1101 9 0.2118336752858454 1101 10-11 0.09033064486899178 1101 12-13 0.20365543310853695 1101 14-15 0.2597544128487641 1101 16-17 0.02317869571572828 1101 18-19 0.142914353971193 1101 20-21 0.15552338291360712 1101 22-23 0.19966126758168912 1101 24-25 0.06183468196187647 1101 26-27 0.05029947841416771 1101 28-29 0.22520160909518694 1101 30-31 0.07096107773383409 1101 32-33 0.18904346373015102 1101 34-35 0.052152730685882887 1101 36-37 0.1318626079301808 1101 38-39 0.1890953734748848 1101 40-41 0.17353472986810914 1101 42-43 0.3246031139367247 1101 44-45 0.05085420414321362 1101 46-47 0.02001930742203939 1101 48-49 0.1966972606266424 1101 50-51 0.24176525264548587 1101 52-53 0.07225273605696714 1101 54-55 0.040304079020415884 1101 56-57 0.291090645761777 1101 58-59 0.16196957843977344 1101 60-61 0.16160423847703953 1101 62-63 0.28537234397540345 1101 64-65 0.3711102051247437 1101 66-67 0.31974270670553295 1101 68-69 0.33481764062553054 1101 70-71 0.4361465019767934 1101 72-73 0.24831932754202057 1101 74-75 0.19366357616229735 1101 76-77 0.2877129424516198 1101 78-79 0.3198940006511535 1101 80-81 0.3193211468589716 1101 82-83 0.11822062423003743 1101 84-85 0.3680315632636173 1101 86-87 0.5429663234721929 1101 88-89 0.6715873204773217 1101 90-91 0.6852390269161752 1101 92-93 0.9840345875718342 1101 94-95 1.171505236422675 1101 96-97 0.9180809984719609 1101 98-99 1.1131720198380641 1101 100-101 1.9448924443661966 1102 1 0.2506595284715587 1102 2 0.3181047003550219 1102 3 0.2581585743276804 1102 4 0.06549131784948514 1102 5 -0.0047630789077004465 1102 6 0.11925641233335682 1102 7 0.1314057455782347 1102 8 0.015172690727197846 1102 9 0.22102154207895808 1102 10-11 0.15554594469443828 1102 12-13 0.15219272746387702 1102 14-15 0.21240631845494562 1102 16-17 0.27461322615182837 1102 18-19 0.2511724388816461 1102 20-21 0.15804884368134253 1102 22-23 0.26535760755338345 1102 24-25 0.14153959819217476 1102 26-27 0.03891600695359898 1102 28-29 -0.10636561970125769 1102 30-31 0.16406468003732044 1102 32-33 0.21567934382745335 1102 34-35 0.16933754631006792 1102 36-37 0.13800961379887156 1102 38-39 0.004542603370836673 1102 40-41 0.05437936764487716 1102 42-43 0.09531017423167043 1102 44-45 0.18713287757193342 1102 46-47 0.2996393544265388 1102 48-49 0.029936103931255786 1102 50-51 0.107627207867516 1102 52-53 0.07311173631809709 1102 54-55 0.05168926848151045 1102 56-57 0.21006458712984966 1102 58-59 0.15625550870269933 1102 60-61 0.2007042123634335 1102 62-63 0.19032568308121967 1102 64-65 0.43238269375157756 1102 66-67 0.36502404647106346 1102 68-69 0.5044289601873686 1102 70-71 0.6383139314356896 1102 72-73 0.6289543692550765 1102 74-75 0.48275667804660216 1102 76-77 0.06281121208149187 1102 78-79 0.5146053078433894 1102 80-81 0.6057083979206936 1102 82-83 0.5437160955806597 1102 84-85 0.7786817921012883 1102 86-87 0.7346247437363544 1102 88-89 0.8754349544470017 1102 90-91 1.2788494393737402 1102 92-93 1.4847028897749972 1102 94-95 1.353094455625797 1102 96-97 1.7090999789417296 1102 98-99 1.7941565028573514 1102 100-101 2.2616213026517684 1103 1 0.12747338089996418 1103 2 0.19941994895823 1103 3 0.13296782148153596 1103 4 0.14499537281598407 1103 5 -0.09966833935799002 1103 6 0.0025416400866902222 1103 7 -0.010131011289487901 1103 8 0.0983557714134875 1103 9 0.12528772793715603 1103 10-11 0.049683989159326813 1103 12-13 0.18999641712476034 1103 14-15 0.11362973362959394 1103 16-17 0.166707092628549 1103 18-19 0.24980285199604424 1103 20-21 0.20503896781600162 1103 22-23 0.26818581993023827 1103 24-25 0.09423474731947579 1103 26-27 0.030077070371341108 1103 28-29 -0.02750400300750755 1103 30-31 -0.007673477209799273 1103 32-33 0.08994261119801905 1103 34-35 0.174332983081527 1103 36-37 0.0836806062577935 1103 38-39 0.0027241135150575246 1103 40-41 -0.03864557058458473 1103 42-43 -0.12450326515653387 1103 44-45 -0.10120071545733111 1103 46-47 0.11866962507915702 1103 48-49 0.13603364077716407 1103 50-51 0.20429036245585053 1103 52-53 0.3208134273096164 1103 54-55 0.2194571695695089 1103 56-57 0.11787500415204732 1103 58-59 0.06155676835164314 1103 60-61 -0.0381437022782265 1103 62-63 0.163898530263765 1103 64-65 0.29904985507716475 1103 66-67 0.3495152526721057 1103 68-69 -0.1232165375508032 1103 70-71 0.029276265632198317 1103 72-73 0.19576052973727087 1103 74-75 0.34794233590587353 1103 76-77 0.18312277300244517 1103 78-79 0.15822767038386232 1103 80-81 0.16479849756973408 1103 82-83 0.1196419115823808 1103 84-85 0.22444429646047226 1103 86-87 0.24659487128415947 1103 88-89 0.22322222143173676 1103 90-91 0.5249447255215607 1103 92-93 0.7429599838022369 1103 94-95 0.7983109802016912 1103 96-97 1.0282638075028423 1103 98-99 1.479558994454333 1103 100-101 2.1983729764538893 1104 1 0.20614269893844295 1104 2 0.2182132779880277 1104 3 0.15609559930053507 1104 4 0.11652280536306137 1104 5 -0.0047630789077004465 1104 6 0.056281385188945876 1104 7 0.20632431235348037 1104 8 0.1389511923558615 1104 9 0.1938771338270584 1104 10-11 0.1962625570722949 1104 12-13 0.2091959847928635 1104 14-15 0.19557678533876555 1104 16-17 0.012941130603515205 1104 18-19 -0.0913899932573301 1104 20-21 0.1754212649625586 1104 22-23 0.21595478453492234 1104 24-25 0.1361107165417934 1104 26-27 0.06606041520549866 1104 28-29 -0.10039384988584032 1104 30-31 0.18578020663884587 1104 32-33 0.11633080962550224 1104 34-35 0.15087934869877984 1104 36-37 0.16786846287595836 1104 38-39 0.16740905288224184 1104 40-41 0.3117083578728952 1104 42-43 0.25274774209268713 1104 44-45 -0.01264996716205502 1104 46-47 0.060768561809815935 1104 48-49 0.17705879665655289 1104 50-51 0.09459789190660217 1104 52-53 0.3087251999445897 1104 54-55 0.09892053883982044 1104 56-57 0.08954341449141623 1104 58-59 0.2686333588655643 1104 60-61 0.27345122647852094 1104 62-63 0.2652442498564582 1104 64-65 0.45029800319783675 1104 66-67 0.5382053711181811 1104 68-69 0.445797038363267 1104 70-71 0.5226787522825944 1104 72-73 0.4682594724038296 1104 74-75 0.6157642784809134 1104 76-77 0.2473931881944118 1104 78-79 0.4174283263015859 1104 80-81 0.4004966715363274 1104 82-83 0.3287323822256134 1104 84-85 0.3992029647397253 1104 86-87 0.5592718664290786 1104 88-89 0.48455547561963996 1104 90-91 0.5448646402423627 1104 92-93 1.0748223251713043 1104 94-95 1.1180238801643405 1104 96-97 1.392053290559538 1104 98-99 1.6692922248986086 1104 100-101 2.1801880778960694 1105 1 0.20674844783837898 1105 2 0.2558840020294042 1105 3 0.13571729196868887 1105 4 0.05619173882592321 1105 5 0.012194080549029707 1105 6 0.07202704684580397 1105 7 -0.030698755631355823 1105 8 0.010094305295645256 1105 9 0.14715827370172008 1105 10-11 0.09246995940004155 1105 12-13 0.005889129163023199 1105 14-15 0.3103281044617603 1105 16-17 0.2252199274871458 1105 18-19 0.23393145368249435 1105 20-21 0.2799034258479409 1105 22-23 0.18227285985351216 1105 24-25 0.25022390396202354 1105 26-27 0.16530799125746398 1105 28-29 0.2329890100871168 1105 30-31 0.17618537264832668 1105 32-33 0.2804639981886581 1105 34-35 0.09480947803950102 1105 36-37 0.07910148571536268 1105 38-39 0.11636423127338702 1105 40-41 -0.07188499031843065 1105 42-43 0.029275924655500773 1105 44-45 0.25584347055819734 1105 46-47 0.44385711925119153 1105 48-49 0.15976447507627256 1105 50-51 0.2600530607733944 1105 52-53 0.2823275010284618 1105 54-55 0.42679641746145336 1105 56-57 0.5403596516097267 1105 58-59 0.3485007798610482 1105 60-61 0.35865990506967904 1105 62-63 0.41017062660180414 1105 64-65 0.5709010852787131 1105 66-67 0.6332355633196443 1105 68-69 0.545288437871811 1105 70-71 0.6169889033388429 1105 72-73 0.8969887331234929 1105 74-75 0.7926105744597329 1105 76-77 0.6560470160321934 1105 78-79 0.87826989820093 1105 80-81 0.946240237835358 1105 82-83 0.793960871472617 1105 84-85 0.9778398392277943 1105 86-87 1.094563406898061 1105 88-89 1.0226014591996524 1105 90-91 1.1577758542164922 1105 92-93 1.3854095968249247 1105 94-95 1.6570222991216177 1105 96-97 1.646850707554794 1105 98-99 2.246412822266077 1105 100-101 2.5526150737244038 1106 1 -0.05338800440333813 1106 2 0.11264163685721229 1106 3 -0.09001370218336291 1106 4 -0.012830353441785292 1106 5 -0.07586634830087036 1106 6 -0.0297110948046182 1106 7 -0.08089368740523639 1106 8 -0.06157561023391622 1106 9 0.12990681171341834 1106 10-11 0.04709146687242338 1106 12-13 -0.16049326200007386 1106 14-15 0.08009743519600221 1106 16-17 0.03211563898811676 1106 18-19 0.03807375387742695 1106 20-21 -0.022443877595854644 1106 22-23 -0.18097437861302978 1106 24-25 0.013536119686520465 1106 26-27 -0.30753963707262244 1106 28-29 -0.03488785800904992 1106 30-31 -0.11185068972983458 1106 32-33 -0.26737550798418397 1106 34-35 -0.23990462202547747 1106 36-37 -0.03769513546722436 1106 38-39 -0.1406045796427975 1106 40-41 -0.018116309356770444 1106 42-43 0.009465983135036993 1106 44-45 2.3105990192817671E-4 1106 46-47 -0.22955093792879921 1106 48-49 -0.10949315975153695 1106 50-51 0.0920543603185564 1106 52-53 -0.10674007531982 1106 54-55 -0.0039215812389983284 1106 56-57 0.00559681860128336 1106 58-59 -0.061327030405358585 1106 60-61 -0.08108993248717411 1106 62-63 -0.2502582233007331 1106 64-65 -0.020659993343763006 1106 66-67 -0.1561812657901669 1106 68-69 -0.3173252723296187 1106 70-71 -0.14750914734341336 1106 72-73 -0.12831178669458154 1106 74-75 0.030679125466878787 1106 76-77 -0.02366383516988435 1106 78-79 0.06310583062458619 1106 80-81 0.009438743358423096 1106 82-83 0.12338744717112249 1106 84-85 0.12572807834409971 1106 86-87 0.19741428409104245 1106 88-89 0.38169827531401523 1106 90-91 0.18807150074021095 1106 92-93 0.48207139019724465 1106 94-95 0.5698877961976407 1106 96-97 0.6585384918302957 1106 98-99 0.9264729262296889 1106 100-101 1.6903018833410357 1107 1 0.21467492104019925 1107 2 0.2603118589365039 1107 3 0.17176471878463673 1107 4 0.11823082109503247 1107 5 -0.06083847672451981 1107 6 0.049711731301755435 1107 7 0.16136123032001137 1107 8 0.16601546371509102 1107 9 0.36430944879332827 1107 10-11 0.217294563118692 1107 12-13 0.35072795077083185 1107 14-15 0.3223769192145127 1107 16-17 0.28995786978620686 1107 18-19 0.07044096189586213 1107 20-21 0.1837845710650612 1107 22-23 0.2672298253497445 1107 24-25 0.21909011193250905 1107 26-27 0.2549179302390243 1107 28-29 0.154961572946263 1107 30-31 0.27910511774188507 1107 32-33 0.21694896361717753 1107 34-35 0.3559150881815967 1107 36-37 0.2730952738518866 1107 38-39 0.09360376039043672 1107 40-41 0.03874795963967159 1107 42-43 0.2412858094736734 1107 44-45 0.3984852897709672 1107 46-47 0.32782475662731514 1107 48-49 0.20720990969514475 1107 50-51 0.3942242340031399 1107 52-53 0.3038989163008381 1107 54-55 0.3529395611382995 1107 56-57 0.41548765492723305 1107 58-59 0.40466651982601576 1107 60-61 0.36438852957353873 1107 62-63 0.28613562351279853 1107 64-65 0.33107237699086767 1107 66-67 0.33662416023402386 1107 68-69 0.5305881560529428 1107 70-71 0.4704727093323626 1107 72-73 0.5184416659862734 1107 74-75 0.5716434194762776 1107 76-77 0.5951275925337463 1107 78-79 0.6684807122597185 1107 80-81 0.6009850351881205 1107 82-83 0.5660955013420335 1107 84-85 0.7208040998552399 1107 86-87 0.8907431954915879 1107 88-89 1.0363631910739635 1107 90-91 1.0554112838421723 1107 92-93 0.9341168691059636 1107 94-95 0.8394648986256819 1107 96-97 1.279303039542505 1107 98-99 1.668190735452324 1107 100-101 2.628216160377356 1108 1 0.12823592722059374 1108 2 0.1313975722798162 1108 3 0.13220852003885852 1108 4 0.035483986539226464 1108 5 -0.0783592257937471 1108 6 -0.09084594760614095 1108 7 0.028080671569121307 1108 8 0.027218311893335567 1108 9 0.046573478380082634 1108 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0.05039011859249598 2316 60-61 0.14912324290143886 2316 62-63 0.17229388346189722 2316 64-65 0.17517458955222054 2316 66-67 0.23683430376048875 2316 68-69 0.21128034070581947 2316 70-71 0.24541895282153092 2316 72-73 0.0011272846304564155 2316 74-75 0.1245493958722399 2316 76-77 0.19028003447573738 2316 78-79 -0.04354746572169077 2316 80-81 0.11983447985064899 2316 82-83 -0.07598964584632029 2316 84-85 -0.2593621400794497 2316 86-87 -0.009035333542787072 2316 88-89 -0.06183398839368692 2316 90-91 -0.3629169033183679 2316 92-93 -0.2486884688520874 2316 94-95 -0.5909309172526527 2316 96-97 -0.7806796568176786 2316 98-99 -0.765912863743818 2316 100-101 -1.4485995011743853 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 6.0 5 25.0 6 26.0 7 70.0 8 184.0 9 400.0 10 668.0 11 1068.0 12 1651.0 13 2465.0 14 3267.0 15 4484.0 16 5956.0 17 8350.0 18 7849.0 19 5001.0 20 4257.0 21 4262.0 22 4455.0 23 4896.0 24 5583.0 25 6551.0 26 7864.0 27 9823.0 28 12342.0 29 15119.0 30 19091.0 31 24148.0 32 31364.0 33 41649.0 34 57594.0 35 83494.0 36 130877.0 37 196980.0 38 152513.0 39 12742.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.791294957056294 19.674264695701936 15.224710285722198 22.309730061519577 2 15.597176731097475 20.535821377496884 40.310813304424045 23.556188586981595 3 19.597038335563038 24.439036767080317 30.11775153388384 25.846173363472808 4 13.262674724362228 17.323199704756192 36.82128523319648 32.592840337685104 5 13.596808947497127 37.35171710553636 34.36056678555444 14.690907161412072 6 31.777113991788532 36.69765188909905 17.179037689717212 14.346196429395212 7 28.38630806845966 32.87343728375698 20.483231074410664 18.2570235733727 8 26.041080407805506 35.89288185634544 19.236287309129494 18.829750426719567 9 25.952276606541496 17.372906767541636 20.269294644092817 36.40552198182405 10-11 24.749907736310377 27.083602435761406 27.09167550860359 21.07481431932463 12-13 25.699695803238036 24.393523924859075 27.853120539769037 22.053659732133845 14-15 22.521393643031786 25.979206070950777 26.28557918531162 25.213821100705818 16-17 21.739285879042303 29.274692069935877 27.0462356414633 21.93978640955852 18-19 21.586820131937078 27.43847165198136 29.871638141809292 21.10307007427227 20-21 22.090752871707338 26.493922129445956 30.36161599852378 21.05370900032292 22-23 21.914951036643803 26.330014520048394 30.263459877265802 21.491574566042 24-25 21.828235455579755 26.659931390136855 30.196470553636274 21.315362600647116 26-27 21.569172447159595 26.92889892782404 30.12034227976221 21.381586345254153 28-29 21.46380957708208 27.07115255642779 30.16528854132848 21.299749325161653 30-31 21.564841917336157 26.838300591180953 30.10865227811108 21.48820521337181 32-33 21.56675277944365 26.818171333671632 30.0950316003137 21.520044286571018 34-35 21.924851224800477 26.952184342851872 29.915578723993175 21.207385708354476 36-37 22.030377819809015 26.70203441435623 29.77406929003091 21.493518475803846 38-39 21.647771831895557 27.11791299534068 29.829139179775797 21.40517599298796 40-41 21.95898878996171 27.02939751810675 29.680940628315728 21.330673063615812 42-43 21.77525487577149 27.038458300028488 29.57089519960415 21.615391624595873 44-45 21.80156386953914 27.21161830511602 29.516595931171285 21.470221894173548 46-47 22.194261198505327 27.500461318448128 28.847049868524245 21.458227614522304 48-49 22.150724269963558 27.527275453245377 28.712517876089866 21.609482400701204 50-51 22.205275176454307 27.88791115006689 28.513747289754114 21.393066383724683 52-53 22.363853392997186 27.841433316418325 28.302694099737046 21.49201919084744 54-55 22.16046962218019 27.970256493057157 28.354534760345068 21.514739124417588 56-57 22.096404022696866 28.153745905798772 28.346577017114917 21.403273054389444 58-59 22.230301702265074 27.733196475527055 28.33158416755086 21.70491765465701 60-61 22.115664067906078 27.964259353231537 28.552094385754483 21.367982193107903 62-63 21.92773446510126 27.99701296304839 28.73875536282696 21.33649720902339 64-65 21.930387046177977 27.933639341237253 28.531277390782854 21.604696221801913 66-67 21.900689671079945 28.01373575679291 28.374832772062554 21.710741800064586 68-69 21.96123771739632 27.880991373345022 28.476092171425936 21.681678737832726 70-71 22.015615629469025 27.962298749827006 28.2096807676339 21.812404853070074 72-73 22.0641244545627 27.9575286925824 28.24423827342245 21.73410857943245 74-75 22.065047091990987 28.27520429210932 27.927888965198694 21.731859650701 76-77 22.0795082345343 28.044644092817272 28.175139548830558 21.700708123817872 78-79 22.190397656502284 28.055312081930158 28.116148452276608 21.638141809290953 80-81 22.110935553812798 28.35418877150897 27.906248558379847 21.62862711629838 82-83 22.086729071555723 28.330850862002848 27.957182703546792 21.625237362894637 84-85 21.994433029996706 28.398985789637273 27.95888722878032 21.647693951585698 86-87 22.076682659039534 28.371949531761775 27.89390828989251 21.657459519306176 88-89 21.994394980855283 28.32495271485907 27.69652165890114 21.984130645384507 90-91 22.000287190441288 28.3846806390391 27.693693517050633 21.92133865346898 92-93 22.073616589394042 28.094480197448853 27.926268078333678 21.905635134823427 94-95 22.013655026064495 28.171794990081654 27.89235134013009 21.92219864372376 96-97 22.04064215527979 28.21908013101444 27.792648890529133 21.947628823176636 98-99 21.852853084897685 28.307801249943775 27.58946993295882 22.24987573219972 100-101 21.732781530284576 28.41335861661684 27.80561399623519 22.048245856863396 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 327.0 1 228.5 2 174.5 3 229.0 4 192.0 5 175.5 6 284.0 7 564.5 8 935.5 9 1191.5 10 1138.5 11 1025.5 12 1060.5 13 1150.0 14 1287.5 15 1380.0 16 1430.0 17 1508.0 18 1605.5 19 1706.0 20 1911.0 21 2221.0 22 2627.0 23 3147.0 24 3813.5 25 4677.0 26 5798.0 27 7222.5 28 8536.5 29 9879.5 30 11418.5 31 13106.0 32 14908.0 33 16432.0 34 18075.0 35 19552.5 36 21458.5 37 23623.5 38 24986.0 39 26067.0 40 26911.0 41 28447.5 42 29680.0 43 30478.5 44 31660.5 45 32490.0 46 32592.5 47 33015.0 48 34003.0 49 33424.0 50 32245.5 51 30809.0 52 29007.0 53 28026.5 54 27501.0 55 25171.5 56 21726.5 57 19303.5 58 17359.5 59 15084.5 60 12191.5 61 9784.0 62 7969.0 63 5949.0 64 4167.0 65 2984.0 66 2156.0 67 1652.0 68 1226.0 69 859.5 70 635.0 71 457.5 72 335.5 73 270.0 74 214.5 75 154.5 76 98.0 77 59.0 78 36.0 79 21.5 80 13.0 81 7.0 82 5.0 83 4.0 84 2.0 85 0.0 86 0.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04197997877935138 2 0.0 3 0.0 4 0.0 5 0.01799141947686488 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004901508511325368 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.459888360935554E-4 24-25 4.03653642109148E-4 26-27 0.001326290538358629 28-29 0.002364257046639295 30-31 2.3065922406237025E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.010264335470775475 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 5.766480601559256E-5 74-75 5.766480601559256E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.766480601559256E-5 84-85 1.729944180467777E-4 86-87 0.0 88-89 0.0 90-91 0.006862111915855515 92-93 0.0023065922406237025 94-95 0.0 96-97 0.0 98-99 1.1532961203118512E-4 100-101 0.011994279651243254 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 867080.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.55786258396816 #Duplication Level Percentage of deduplicated Percentage of total 1 85.40660224123225 60.261073047004906 2 9.154376356124182 12.918264579546742 3 2.3429725487234982 4.959454053925268 4 0.9820775412463196 2.7717316880823657 5 0.552659551502253 1.9497238345306718 6 0.3427745229529978 1.4511262612681715 7 0.23354596732259447 1.1534952988571279 8 0.15913664330790966 0.8982673128474757 9 0.12925032522191343 0.8207664017346863 >10 0.6484336566729231 7.94849520447511 >50 0.0328828542442189 1.5869259630592116 >100 0.014137097517173888 1.8447642925637702 >500 4.931545422192639E-4 0.20668782671004526 >1k 6.575393896256852E-4 1.2292242353945342 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3963 0.4570512524795867 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3485 0.40192369792868016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2114 0.24380679983392536 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1036 0.1194814780643078 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.1532961203118512E-4 7 0.0 0.0 0.0 0.0 1.1532961203118512E-4 8 0.0 0.0 0.0 0.0 1.1532961203118512E-4 9 0.0 0.0 0.0 0.0 1.1532961203118512E-4 10-11 0.0 0.0 0.0 0.0 1.1532961203118512E-4 12-13 0.0 0.0 0.0 0.0 1.1532961203118512E-4 14-15 0.0 0.0 0.0 0.0 1.1532961203118512E-4 16-17 0.0 0.0 0.0 0.0 1.1532961203118512E-4 18-19 0.0 0.0 0.0 0.0 1.1532961203118512E-4 20-21 0.0 0.0 0.0 1.1532961203118512E-4 1.1532961203118512E-4 22-23 0.0 0.0 0.0 4.0365364210914794E-4 1.1532961203118512E-4 24-25 0.0 0.0 0.0 4.613184481247405E-4 1.1532961203118512E-4 26-27 0.0 0.0 0.0 5.189832541403331E-4 1.1532961203118512E-4 28-29 5.766480601559256E-5 0.0 0.0 0.0015569497624209993 1.1532961203118512E-4 30-31 1.1532961203118512E-4 0.0 0.0 0.003690547584997924 1.1532961203118512E-4 32-33 1.1532961203118512E-4 0.0 0.0 0.006862111915855515 1.1532961203118512E-4 34-35 2.3065922406237025E-4 0.0 0.0 0.01078331872491581 1.1532961203118512E-4 36-37 2.3065922406237025E-4 0.0 0.0 0.014531531115929325 1.1532961203118512E-4 38-39 2.3065922406237025E-4 0.0 0.0 0.01764543064077132 1.1532961203118512E-4 40-41 2.3065922406237025E-4 0.0 0.0 0.01977902846334825 1.1532961203118512E-4 42-43 2.3065922406237025E-4 0.0 0.0 0.02202795589795636 1.1532961203118512E-4 44-45 2.3065922406237025E-4 0.0 0.0 0.02491119619873599 1.1532961203118512E-4 46-47 2.3065922406237025E-4 0.0 0.0 0.029351386261936615 1.1532961203118512E-4 48-49 2.3065922406237025E-4 0.0 0.0 0.03454121880333995 2.883240300779628E-4 50-51 2.3065922406237025E-4 0.0 0.0 0.038866079254509384 3.459888360935554E-4 52-53 2.3065922406237025E-4 0.0 0.0 0.046304839230520825 3.459888360935554E-4 54-55 2.3065922406237025E-4 0.0 0.0 0.06106702957051252 3.459888360935554E-4 56-57 2.3065922406237025E-4 0.0 0.0 0.09278267287908844 3.459888360935554E-4 58-59 2.3065922406237025E-4 0.0 0.0 0.12640125478617892 3.459888360935554E-4 60-61 2.3065922406237025E-4 0.0 0.0 0.15396503206163215 3.459888360935554E-4 62-63 2.3065922406237025E-4 0.0 0.0 0.19058218388153342 3.459888360935554E-4 64-65 2.3065922406237025E-4 0.0 0.0 0.23682935830603866 3.459888360935554E-4 66-67 3.459888360935554E-4 0.0 0.0 0.3105249803939659 3.459888360935554E-4 68-69 3.459888360935554E-4 0.0 0.0 0.4054989159016469 3.459888360935554E-4 70-71 3.459888360935554E-4 0.0 0.0 0.4873829404437884 4.613184481247405E-4 72-73 3.459888360935554E-4 0.0 0.0 0.5786663283664714 4.613184481247405E-4 74-75 3.459888360935554E-4 0.0 0.0 0.6779651243253217 4.613184481247405E-4 76-77 3.459888360935554E-4 0.0 0.0 0.7970429487475204 4.613184481247405E-4 78-79 3.459888360935554E-4 0.0 0.0 0.9130645384508926 5.189832541403331E-4 80-81 3.459888360935554E-4 0.0 0.0 1.0187064630714582 6.343128661715181E-4 82-83 3.459888360935554E-4 5.766480601559256E-5 0.0 1.114545370669373 6.919776721871107E-4 84-85 3.459888360935554E-4 1.1532961203118512E-4 0.0 1.2739885593024867 6.919776721871107E-4 86-87 3.459888360935554E-4 1.1532961203118512E-4 0.0 1.4579969552982424 6.919776721871107E-4 88-89 3.459888360935554E-4 1.1532961203118512E-4 0.0 1.7060709507773217 6.919776721871107E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2405 0.0 46.429283 1 GTATCAA 3870 0.0 35.974586 1 ATAACGC 60 0.003948403 31.669973 3 ATCAACG 4955 0.0 27.994848 3 TATCAAC 4985 0.0 27.921673 2 TCAACGC 5035 0.0 27.455698 4 CAACGCA 5145 0.0 26.868694 5 AACGCAG 5245 0.0 26.351862 6 ACACCGT 130 1.0022697E-5 25.575169 6 ACGACCC 140 1.7781842E-5 23.75111 8 ACGCAGA 5865 0.0 23.487885 7 CGCAGAG 5970 0.0 23.07478 8 TAGGGTG 240 5.9153535E-9 21.773108 5 GAGTACT 4165 0.0 21.55563 12-13 GTGGTAT 1425 0.0 21.007038 1 TGGTATC 1290 0.0 20.990564 2 GCAGAGT 6535 0.0 20.861725 9 GTACTTT 4330 0.0 20.567295 14-15 GGACCGA 185 6.552931E-6 20.539133 6 TAAGGTG 440 0.0 20.513506 5 >>END_MODULE