##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937839_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 254557 Sequences flagged as poor quality 0 Sequence length 101 %GC 30 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.900352376874334 33.0 31.0 34.0 30.0 34.0 2 31.882116775417686 33.0 31.0 34.0 30.0 34.0 3 31.749733851357455 33.0 31.0 34.0 30.0 34.0 4 35.4745066920179 37.0 35.0 37.0 33.0 37.0 5 35.492133392521126 37.0 35.0 37.0 33.0 37.0 6 35.678394229976 37.0 35.0 37.0 35.0 37.0 7 35.66685261061373 37.0 35.0 37.0 35.0 37.0 8 35.68313187223294 37.0 35.0 37.0 35.0 37.0 9 37.235401108592576 39.0 37.0 39.0 34.0 39.0 10-11 37.168166658155144 39.0 37.0 39.0 34.0 39.0 12-13 36.989786177555516 39.0 37.0 39.0 33.5 39.0 14-15 37.66887376893976 40.0 37.0 41.0 32.5 41.0 16-17 37.758639518850394 39.5 37.5 41.0 32.5 41.0 18-19 38.139424568957054 40.0 38.0 41.0 33.5 41.0 20-21 38.3078210381172 40.0 38.0 41.0 34.0 41.0 22-23 38.19072152798783 40.0 38.0 41.0 34.0 41.0 24-25 38.09523800170492 40.0 37.5 41.0 34.0 41.0 26-27 37.8495602163759 40.0 36.5 41.0 34.0 41.0 28-29 37.5998145798387 39.5 36.0 41.0 33.0 41.0 30-31 36.85811232847653 38.5 35.0 40.5 33.0 41.0 32-33 36.08421296605475 37.0 35.0 40.0 32.5 41.0 34-35 35.727361651810796 36.0 35.0 40.0 31.0 41.0 36-37 35.389272343718694 36.0 35.0 40.0 31.0 41.0 38-39 35.077805756667466 35.5 35.0 40.0 30.0 41.0 40-41 34.62738011525906 35.0 35.0 40.0 28.5 41.0 42-43 34.02643022977172 35.0 35.0 40.0 23.5 41.0 44-45 33.26503494305794 35.0 33.5 40.0 18.0 41.0 46-47 32.200707503623946 35.0 33.0 40.0 3.5 41.0 48-49 31.367171203306135 35.0 32.5 40.0 2.0 41.0 50-51 30.212188232890867 35.0 29.0 39.0 2.0 40.5 52-53 30.412909485891177 35.0 31.0 39.0 2.0 40.0 54-55 30.72063624257043 35.0 32.0 40.0 2.0 41.0 56-57 30.50541136169895 35.0 31.0 40.0 2.0 41.0 58-59 30.313153831951194 35.0 31.0 39.0 2.0 41.0 60-61 29.99055810682873 35.0 31.0 39.0 2.0 41.0 62-63 29.603652620041878 35.0 29.0 39.0 2.0 41.0 64-65 29.1990300797071 35.0 28.0 38.5 2.0 40.0 66-67 28.756973880113293 35.0 27.5 38.0 2.0 40.0 68-69 28.3103234246161 35.0 26.0 37.0 2.0 40.0 70-71 27.872103301028847 35.0 25.5 37.0 2.0 39.5 72-73 27.42908464508931 35.0 24.5 36.0 2.0 39.0 74-75 26.97464222158495 34.5 22.5 36.0 2.0 39.0 76-77 26.52473512808526 34.0 20.0 35.0 2.0 38.5 78-79 26.025165287145903 34.0 16.5 35.0 2.0 37.5 80-81 25.506051689798355 33.0 9.0 35.0 2.0 37.0 82-83 25.081193995843762 33.0 3.5 35.0 2.0 36.0 84-85 24.60088703119537 33.0 2.0 35.0 2.0 36.0 86-87 24.10738066523411 33.0 2.0 35.0 2.0 35.5 88-89 23.745479401470007 32.0 2.0 35.0 2.0 35.0 90-91 23.26405873733584 32.0 2.0 35.0 2.0 35.0 92-93 22.764697101238625 31.0 2.0 35.0 2.0 35.0 94-95 22.170038537537764 31.0 2.0 35.0 2.0 35.0 96-97 21.505503678940276 31.0 2.0 34.5 2.0 35.0 98-99 20.719326123422256 29.5 2.0 34.0 2.0 35.0 100-101 18.68618423378654 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15081488589911274 1101 2 0.4873782097377166 1101 3 0.6164025475859347 1101 4 0.2848247882498214 1101 5 0.19762259443427155 1101 6 0.023420636990103105 1101 7 0.18922466371209623 1101 8 0.20400229564372552 1101 9 0.1611634267308375 1101 10-11 0.32555820308356687 1101 12-13 0.1228635846292363 1101 14-15 0.3724519319426136 1101 16-17 0.45957535268928495 1101 18-19 0.3057106932732836 1101 20-21 0.5100417695375441 1101 22-23 0.4726208319680367 1101 24-25 0.6794538002589761 1101 26-27 0.734285141971057 1101 28-29 0.7065591100812867 1101 30-31 0.8453095973046345 1101 32-33 1.1143886390343383 1101 34-35 1.1397033175548685 1101 36-37 0.9458068465854197 1101 38-39 0.9323295959472517 1101 40-41 0.958432157774773 1101 42-43 1.4086630044382602 1101 44-45 1.7053634103343143 1101 46-47 2.0399667780684716 1101 48-49 2.608457557424547 1101 50-51 2.777578563996663 1101 52-53 2.5086401093738857 1101 54-55 2.586163484753552 1101 56-57 2.417203459758568 1101 58-59 2.3976772718702257 1101 60-61 2.4700155537388575 1101 62-63 2.334103632400822 1101 64-65 2.6049700649528447 1101 66-67 2.686714753258709 1101 68-69 2.7251340871356504 1101 70-71 2.707816376788049 1101 72-73 2.6182090189953726 1101 74-75 2.173766524688599 1101 76-77 2.079455557894722 1101 78-79 2.295648634245566 1101 80-81 2.1682152442471256 1101 82-83 2.1418970133789763 1101 84-85 2.4042507683882093 1101 86-87 2.248378025188856 1101 88-89 2.4380815037562087 1101 90-91 2.0182109428298567 1101 92-93 1.745837144103934 1101 94-95 1.642451348229212 1101 96-97 1.9415487198264962 1101 98-99 2.040033354037668 1101 100-101 1.556887111714616 1102 1 0.4541986605943613 1102 2 0.5945976296620579 1102 3 0.5635862382122454 1102 4 0.3759975499143877 1102 5 0.3544426869100974 1102 6 0.2439754919711845 1102 7 0.32969334803323846 1102 8 0.08470847471055976 1102 9 0.2606485044946041 1102 10-11 0.07824840905245622 1102 12-13 0.15025954932027474 1102 14-15 0.3694517217702824 1102 16-17 0.6257901488221123 1102 18-19 0.283973618872281 1102 20-21 0.26349910455225967 1102 22-23 0.16543293789489866 1102 24-25 0.3858640566692415 1102 26-27 -0.08642734226602045 1102 28-29 0.37552190957687515 1102 30-31 0.652161215631665 1102 32-33 0.6503491266341754 1102 34-35 0.701972127979424 1102 36-37 0.9060632568418256 1102 38-39 0.5917348082213181 1102 40-41 0.5238588076276471 1102 42-43 1.0851026849762988 1102 44-45 1.208337348963994 1102 46-47 1.511209947467382 1102 48-49 1.9499193060584368 1102 50-51 2.361799875472073 1102 52-53 2.2861253973099878 1102 54-55 2.3740523035849996 1102 56-57 2.6627845862823136 1102 58-59 2.508763862874847 1102 60-61 2.544805381397534 1102 62-63 2.2760645403453346 1102 64-65 2.112021347004127 1102 66-67 2.3861525422456786 1102 68-69 2.371414036694283 1102 70-71 2.284912845892716 1102 72-73 2.315256097599832 1102 74-75 2.097258538139627 1102 76-77 2.0359919177097723 1102 78-79 2.0372102147415703 1102 80-81 1.3187196578662927 1102 82-83 1.066129884333158 1102 84-85 1.02072827994769 1102 86-87 0.7462605388500592 1102 88-89 0.544418199847005 1102 90-91 0.5943248562388526 1102 92-93 0.7132972113590839 1102 94-95 0.6721119198979828 1102 96-97 0.7500606996499535 1102 98-99 0.48250918421841504 1102 100-101 0.7826489864519033 1103 1 0.472382857349988 1103 2 0.5554165726238587 1103 3 0.5752293569982214 1103 4 0.10362706325818749 1103 5 0.15670589988759787 1103 6 0.15658669029667038 1103 7 0.385488743114351 1103 8 0.43112396398319675 1103 9 0.3164438995757166 1103 10-11 0.3978454786495291 1103 12-13 0.32353270473628726 1103 14-15 0.4802248771862949 1103 16-17 0.5159654497117074 1103 18-19 0.3419602750732196 1103 20-21 0.3257047560436206 1103 22-23 0.24000122674889468 1103 24-25 0.2570048206671416 1103 26-27 -0.053525720078681616 1103 28-29 0.07760851344919217 1103 30-31 0.6547776468195252 1103 32-33 0.7954302360010033 1103 34-35 0.7416437658653408 1103 36-37 0.8121333771976609 1103 38-39 0.7668394654688271 1103 40-41 0.3286730410133103 1103 42-43 0.7558248199841486 1103 44-45 0.67295011714819 1103 46-47 1.6800024644741818 1103 48-49 1.6595281495958467 1103 50-51 1.6181774264924798 1103 52-53 1.7439027390159048 1103 54-55 1.6949902941658443 1103 56-57 1.8460982963817365 1103 58-59 1.564951722634138 1103 60-61 1.788198892648186 1103 62-63 1.8152455973835337 1103 64-65 1.937963262231758 1103 66-67 1.9194793345010446 1103 68-69 1.8606866688240586 1103 70-71 1.8498984555211813 1103 72-73 1.6797903728483945 1103 74-75 1.671859532383479 1103 76-77 1.4636475953654475 1103 78-79 1.4705566302308455 1103 80-81 1.412126251272884 1103 82-83 1.9332151799898796 1103 84-85 1.4563640727263376 1103 86-87 1.6659033959929133 1103 88-89 1.3290205023064487 1103 90-91 1.0467712193994991 1103 92-93 0.5931049036667737 1103 94-95 0.6770504337650713 1103 96-97 0.7859058069236333 1103 98-99 1.224398247536051 1103 100-101 1.2673755693927689 1104 1 0.34276930980330533 1104 2 0.4780537125862274 1104 3 0.29067298125643504 1104 4 0.19705592416424622 1104 5 0.24253597659913595 1104 6 0.413642704191588 1104 7 0.39590993286684295 1104 8 0.45022947852943673 1104 9 0.532538848193461 1104 10-11 0.4799123479504388 1104 12-13 0.5417625498658296 1104 14-15 0.902355431535689 1104 16-17 0.734014371408044 1104 18-19 0.6542341207817159 1104 20-21 0.42477842806562194 1104 22-23 0.725853014272424 1104 24-25 0.36704245274125213 1104 26-27 0.43764521638100007 1104 28-29 0.42110974372853605 1104 30-31 0.29618740221103934 1104 32-33 0.5224167752975646 1104 34-35 0.7253968415637075 1104 36-37 0.8805586196350603 1104 38-39 1.012424933698675 1104 40-41 0.5245407497989518 1104 42-43 0.3285560401317795 1104 44-45 0.8513406222864148 1104 46-47 0.8334617205170254 1104 48-49 0.937064696129351 1104 50-51 0.9845835634153346 1104 52-53 0.9798992652859866 1104 54-55 0.9033417198424978 1104 56-57 0.8187788579680699 1104 58-59 0.7245985600925273 1104 60-61 0.8660683382987884 1104 62-63 0.9688495921729405 1104 64-65 1.1553246748819248 1104 66-67 1.2750300654317108 1104 68-69 0.7670768844847942 1104 70-71 0.5899114820083717 1104 72-73 0.3907470959631709 1104 74-75 0.7132909985869809 1104 76-77 0.5763628942509627 1104 78-79 0.31087360808581366 1104 80-81 0.33031267477845816 1104 82-83 0.6584648543277041 1104 84-85 0.4390316078254841 1104 86-87 0.5232995459422547 1104 88-89 0.6713276379266553 1104 90-91 0.9289538796976622 1104 92-93 1.121473697993018 1104 94-95 0.4507875882013117 1104 96-97 0.5664818671349501 1104 98-99 1.0771627575953957 1104 100-101 0.8843606613018764 1105 1 0.20429615498843745 1105 2 0.6139380800861609 1105 3 0.35736122115072533 1105 4 0.3963251310784699 1105 5 0.22208186015763687 1105 6 0.09232296199166257 1105 7 0.018284066566920387 1105 8 0.23943207810336986 1105 9 0.2211023408990087 1105 10-11 0.4033678396851954 1105 12-13 0.37463071047851315 1105 14-15 0.6504610324849125 1105 16-17 0.42476889504420257 1105 18-19 0.4775426449032807 1105 20-21 0.6318207385582966 1105 22-23 0.7161787700095488 1105 24-25 0.7729899219319876 1105 26-27 0.9467329385178331 1105 28-29 0.9164512748883737 1105 30-31 0.9330425649540786 1105 32-33 0.8166014421260357 1105 34-35 0.9227177163580862 1105 36-37 0.4568334625550037 1105 38-39 0.23876854128166514 1105 40-41 0.2626956995063665 1105 42-43 0.45126725551349267 1105 44-45 0.1328645011527385 1105 46-47 0.11537661413404621 1105 48-49 -0.337232151482759 1105 50-51 0.12106793630857737 1105 52-53 0.054448249995964204 1105 54-55 -0.2310527466246164 1105 56-57 -0.05704699215192832 1105 58-59 0.31039933133541453 1105 60-61 0.4585837766398093 1105 62-63 0.7548229492368215 1105 64-65 0.6593426885269906 1105 66-67 0.38691421719937225 1105 68-69 0.7447688187943129 1105 70-71 0.6818673647578812 1105 72-73 0.30310585873856866 1105 74-75 0.3286066418736624 1105 76-77 0.4857823047625196 1105 78-79 0.4279555593842552 1105 80-81 0.8155754636574564 1105 82-83 0.3542014452184077 1105 84-85 0.3223271880104761 1105 86-87 0.2881648481022765 1105 88-89 0.22301574298827376 1105 90-91 0.02967388616962907 1105 92-93 0.33676776805207354 1105 94-95 0.5111791422826961 1105 96-97 0.7779197227561276 1105 98-99 0.13199246392983355 1105 100-101 0.0678139957138697 1106 1 -0.007061094143654856 1106 2 -0.039599694752659786 1106 3 -0.19965233480893474 1106 4 -0.0357917477444758 1106 5 0.09259764789114655 1106 6 -0.15234557826292416 1106 7 -0.1275251580983081 1106 8 0.011129879392839825 1106 9 0.09147347662392491 1106 10-11 -0.1511969644146589 1106 12-13 -0.14091938044560237 1106 14-15 -0.3773431500245934 1106 16-17 -0.13898131160151905 1106 18-19 -0.09792437443876167 1106 20-21 0.07174882584545372 1106 22-23 0.12592624893168392 1106 24-25 -0.25369837365406767 1106 26-27 -0.2618035964466259 1106 28-29 -0.4158660502893383 1106 30-31 -0.1176269671999961 1106 32-33 -0.13567372732345717 1106 34-35 -0.32673467246650745 1106 36-37 -0.5150627186319738 1106 38-39 -0.6122088930049898 1106 40-41 -0.6428775357391316 1106 42-43 -0.9950255201519056 1106 44-45 -1.6822702318609792 1106 46-47 -1.6726668641268212 1106 48-49 -1.6542473606082346 1106 50-51 -1.3365696897453176 1106 52-53 -1.2232112805049482 1106 54-55 -1.278260962858706 1106 56-57 -1.5635253356797847 1106 58-59 -1.5454446098118844 1106 60-61 -1.377783804778609 1106 62-63 -1.1848468308921234 1106 64-65 -1.406442978525412 1106 66-67 -1.3281729872972399 1106 68-69 -1.8027080368843116 1106 70-71 -1.6074366524350445 1106 72-73 -1.6037795768706289 1106 74-75 -1.5798596335437587 1106 76-77 -1.1570822182991485 1106 78-79 -1.3230880015349058 1106 80-81 -1.5393489040066193 1106 82-83 -1.3655869495575885 1106 84-85 -1.2450186542775086 1106 86-87 -1.0789206205702335 1106 88-89 -0.6690015325944678 1106 90-91 -0.5531807178971597 1106 92-93 -0.185880603579605 1106 94-95 -0.0266060288199661 1106 96-97 0.41519447891417016 1106 98-99 0.4108815365038119 1106 100-101 -0.020723365833493546 1107 1 0.16798526893073173 1107 2 0.31301724322385027 1107 3 0.21491082200128275 1107 4 0.3823774259625097 1107 5 0.3024163167557674 1107 6 0.4957071726128177 1107 7 0.5489636117347843 1107 8 0.5429658571385616 1107 9 0.5059851189070486 1107 10-11 0.7481050137431922 1107 12-13 0.754570522707084 1107 14-15 0.8054175134825172 1107 16-17 0.9289405923754046 1107 18-19 0.7539198456313017 1107 20-21 0.9366519785345133 1107 22-23 0.8708649466444385 1107 24-25 0.9410651746665621 1107 26-27 0.7582226759621946 1107 28-29 0.7808035355338561 1107 30-31 1.0559769891156847 1107 32-33 0.761207068060834 1107 34-35 0.6947340067010188 1107 36-37 0.665238126206269 1107 38-39 0.4590485766014325 1107 40-41 0.7591547123548352 1107 42-43 0.3195479395637335 1107 44-45 0.7106310663051048 1107 46-47 0.8878884624752779 1107 48-49 0.7503734924723346 1107 50-51 0.9112667001166201 1107 52-53 1.279737926171336 1107 54-55 1.4377523278892888 1107 56-57 1.2280340090554311 1107 58-59 1.2694121798027886 1107 60-61 1.3346492263361647 1107 62-63 1.574232604508662 1107 64-65 1.975112245047633 1107 66-67 2.142631154470738 1107 68-69 1.891282793529868 1107 70-71 1.7595528386014259 1107 72-73 1.4699377114046683 1107 74-75 1.1458899028254947 1107 76-77 0.7783056860898903 1107 78-79 1.0590081245726566 1107 80-81 1.2555588849898776 1107 82-83 1.2150847998757506 1107 84-85 1.3263547230148873 1107 86-87 1.308365594142316 1107 88-89 1.2954663223406229 1107 90-91 1.823377596547516 1107 92-93 1.6140369731770434 1107 94-95 2.009827620469135 1107 96-97 2.301373131294138 1107 98-99 1.8884212026896776 1107 100-101 1.4291658779332508 1108 1 0.034967891363592685 1108 2 0.06788822795265403 1108 3 0.12341529804130502 1108 4 0.2858266097434452 1108 5 0.12303243049984047 1108 6 0.23799258598828033 1108 7 0.3405907839306721 1108 8 0.17103326103534755 1108 9 0.41599038483648343 1108 10-11 0.08871849452254565 1108 12-13 0.5423535664142989 1108 14-15 0.7462679610865166 1108 16-17 0.6251491231810888 1108 18-19 0.7201915675902271 1108 20-21 0.31691790797106023 1108 22-23 2.6199772396040544E-4 1108 24-25 0.22667602248120033 1108 26-27 -0.0849316157703015 1108 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6 207.0 7 270.0 8 300.0 9 394.0 10 580.0 11 967.0 12 1717.0 13 2647.0 14 3044.0 15 4278.0 16 6346.0 17 8834.0 18 7088.0 19 3459.0 20 2876.0 21 2684.0 22 3027.0 23 3300.0 24 3375.0 25 3523.0 26 3907.0 27 4276.0 28 4751.0 29 5362.0 30 6334.0 31 7707.0 32 9695.0 33 12855.0 34 19432.0 35 37645.0 36 24971.0 37 29072.0 38 24415.0 39 3583.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.28348676299552 17.81899899465789 17.359695637603735 13.53781860474285 2 15.31440641896685 23.0689502971452 42.625360593975124 18.99128268991283 3 18.71864692646288 33.228642473489046 34.25637307251254 13.796337527535535 4 9.282831785866293 19.471286473193878 40.269085864716175 30.976795876223655 5 8.017168667575811 39.26209413600927 34.390543832128586 18.330193364286334 6 20.555096256052913 41.113341994409666 18.903980158261486 19.42758159127593 7 17.96278945254986 43.3318635980694 21.594479044462116 17.110867904918624 8 24.94143769955473 29.17642705007421 20.936760589441857 24.9453746609292 9 27.96108707377472 15.582886816297444 23.24421365101987 33.211812458907964 10-11 21.90702673758893 28.88277746498836 25.28042773842756 23.929768058995148 12-13 29.121313876084038 29.224846177768505 27.81633448412964 13.837505462017816 14-15 24.233435555048167 25.630159473748442 36.34474043688957 13.791664534313822 16-17 13.287492445702565 30.201244515137137 36.11239613456197 20.39886690459833 18-19 12.219088130101785 23.239238395930972 49.70661354675779 14.835059927209453 20-21 14.319734853256078 20.92576993324096 50.72860246882479 14.02589274467817 22-23 13.150767971154831 21.487840784739774 51.08267794021555 14.278713303889848 24-25 13.267673994296139 21.63845243929562 51.95846298887767 13.135410577530571 26-27 13.525928287605316 20.87862345580609 52.71169995650574 12.883748300082857 28-29 12.129569479714169 21.852595523533928 53.42428000551192 12.593554991239985 30-31 12.295867843439126 23.098064152867543 52.56475628170878 12.041311721984552 32-33 11.648015876140901 23.12002394021247 51.89257617161353 13.339384012033106 34-35 12.128018962272323 22.577546086668924 51.714321712904265 13.580113238154487 36-37 12.883006986126144 22.47072714749582 51.9586736199941 12.687592246383941 38-39 13.29258765862576 22.768318335750394 51.48988671626724 12.449207289356602 40-41 12.077631231968764 23.34348789521122 52.06810641036238 12.510774462457642 42-43 11.832885900756567 24.476661339463554 50.35466576392879 13.335786995851079 44-45 12.315002352144585 24.564853251766078 49.2194619789114 13.900682417177936 46-47 12.646721296954262 24.654689471498227 47.5498060119562 15.148783219591316 48-49 12.916128625870051 24.815163301943247 46.76781833643035 15.500889735756354 50-51 13.528421674294993 25.35794084354545 46.582410551700384 14.531226930459177 52-53 12.774513201840065 25.784942340412126 46.100100825508854 15.340443632238957 54-55 12.49571916123115 25.102051259845933 46.58064312959821 15.821586449324709 56-57 13.715933017178614 25.941085027253585 44.25746275999134 16.08551919557646 58-59 13.539270456786065 24.856694760331457 45.343489819572156 16.260544963310316 60-61 13.976998829281731 24.347004830442607 46.003128474032685 15.672867866242978 62-63 15.22694313975888 24.399117689306212 45.342584084169765 15.031355086765144 64-65 14.90134504442818 24.60116953541268 46.263546856184924 14.233938563974217 66-67 14.668440127972548 24.47947205421125 45.609109189861364 15.24297862795484 68-69 14.21312804527672 25.037389504175817 45.6921387583536 15.057343692193859 70-71 14.524460291504573 24.666906733885487 44.97108370671657 15.837549267893372 72-73 14.129850913662937 23.985447504963314 45.29493594422986 16.589765637143884 74-75 13.814172509401178 24.630987777976745 45.657354367833506 15.89748534478857 76-77 13.861464075079159 23.910326926520742 46.00959939546242 16.218609602937676 78-79 13.324713508917977 23.57226778133985 46.91054078574226 16.192477923999913 80-81 13.36595866932707 24.38911124491828 46.066069257016025 16.17886082873863 82-83 12.786174300932876 23.963574328086846 45.779427129963224 17.470824241017056 84-85 13.210283168846704 23.162314460657 45.342431254033464 18.284971116462827 86-87 13.533970687480693 23.473035748366446 45.546555119853146 17.44643844429972 88-89 13.91661091653221 22.343473298965016 45.810869308567156 17.929046475935618 90-91 13.041741443909197 22.492010641982304 46.585238185195685 17.881009728912815 92-93 12.953791970407972 23.683853259746776 46.17418764572204 17.188167124123208 94-95 12.71823932784353 23.731098058892396 45.651545113780585 17.899117499483484 96-97 12.510699085634005 23.438031879831804 44.91628641870367 19.134982615830516 98-99 13.17991302465613 23.276923985123673 44.680729648359865 18.862433341860328 100-101 14.166555189210031 22.260605440624484 45.8142838032436 17.758555566921885 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3674.0 1 2554.0 2 2489.0 3 3054.0 4 1638.5 5 1076.5 6 2983.0 7 7610.0 8 12349.0 9 12445.0 10 7462.5 11 3138.5 12 2196.0 13 2144.0 14 2050.5 15 1912.5 16 1826.0 17 1839.0 18 1834.0 19 1807.5 20 1890.0 21 1992.5 22 2065.5 23 2174.5 24 2297.5 25 2468.5 26 2851.5 27 3274.5 28 3520.0 29 3722.5 30 4012.5 31 4346.0 32 4813.5 33 5392.0 34 5985.0 35 6285.5 36 6691.0 37 7162.5 38 6890.5 39 6259.0 40 5834.5 41 5591.5 42 5198.5 43 5015.0 44 5136.5 45 4975.5 46 4985.0 47 6655.0 48 13156.5 49 12898.5 50 6570.5 51 4294.5 52 2538.5 53 1865.5 54 1549.0 55 1324.5 56 1243.5 57 1145.5 58 971.5 59 900.5 60 940.0 61 957.5 62 837.5 63 506.0 64 240.5 65 165.0 66 125.0 67 102.0 68 81.5 69 69.0 70 63.0 71 52.5 72 39.0 73 42.0 74 42.5 75 30.0 76 19.0 77 13.0 78 12.5 79 8.0 80 4.0 81 2.5 82 2.0 83 2.5 84 3.5 85 2.0 86 0.5 87 0.5 88 1.0 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35826946420644495 2 0.3178070137533048 3 0.3119144238814882 4 0.3178070137533048 5 0.3295921934969378 6 0.2148831106589094 7 0.21331175335975833 8 0.2176329859324238 9 0.2176329859324238 10-11 0.22391841512902805 12-13 0.20820484213751733 14-15 0.20977619943666842 16-17 0.22097212019311982 18-19 0.21232965504778892 20-21 0.1999552163169742 22-23 0.20074089496654973 24-25 0.20368718990245802 26-27 0.19661608205627815 28-29 0.22077570053072593 30-31 0.23000742466323848 32-33 0.23216804094957122 34-35 0.227453969052118 36-37 0.18934855454770444 38-39 0.18090250906476743 40-41 0.19013423319727998 42-43 0.2023122522657008 44-45 0.2095797797742745 46-47 0.21468669099651552 48-49 0.21566878930848496 50-51 0.2237219954666342 52-53 0.2565240790864129 54-55 0.2034907702400641 56-57 0.18149176805194905 58-59 0.18325954501349404 60-61 0.17265288324422426 62-63 0.1773669551416775 64-65 0.1724564635818304 66-67 0.17284930290661815 68-69 0.18659867927419005 70-71 0.1812953483895552 72-73 0.1738314012185876 74-75 0.18325954501349404 76-77 0.18797361691094727 78-79 0.19308052813318827 80-81 0.18070608940237354 82-83 0.17697411581688974 84-85 0.1700994276331038 86-87 0.17029584729549768 88-89 0.17559917818013254 90-91 0.18424164332546344 92-93 0.17068868662028544 94-95 0.17658127649210195 96-97 0.17540275851773865 98-99 0.18149176805194905 100-101 0.1551715332911686 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 254557.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.01302372789868 #Duplication Level Percentage of deduplicated Percentage of total 1 81.44997212774734 32.590596673842406 2 10.249115549787891 8.201962073672766 3 3.2475125495471118 3.89828390105032 4 1.511531151028533 2.419237272462508 5 0.8347336883016974 1.67001094382461 6 0.5468252740888238 1.3128079600278482 7 0.3658830802450606 1.0248061860037585 8 0.2619163029523074 0.838405059580334 9 0.19493770637964314 0.7020042363747726 >10 1.1396358219062748 8.373650532306835 >50 0.09297029073445559 2.4900150264454437 >100 0.07897476309701067 6.64804011879892 >500 0.008997124909786025 2.597215673367111 >1k 0.013995527637444927 11.40766884163817 >5k 0.0 0.0 >10k+ 0.002999041636595341 15.825295500604192 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17134 6.730908990913627 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12310 4.835852088137431 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10119 3.9751411275274298 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3760 1.4770758612020098 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3524 1.3843657805520966 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3320 1.3042265582953916 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2610 1.0253106376960759 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2440 0.9585279524821553 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2366 0.9294578424478604 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1890 0.7424663238488826 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1545 0.6069367567971025 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1421 0.5582246805234191 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1231 0.4835852088137431 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1193 0.4686573144718079 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1146 0.4501938662067827 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1065 0.4183738808989735 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1008 0.39598203938607074 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 917 0.3602336608303838 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 870 0.34177021256535867 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 842 0.3307707114713011 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 833 0.3272351575482112 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 704 0.2765588846505891 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 642 0.2522028465137474 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 571 0.22431125445381583 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 567 0.22273989715466477 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 547 0.2148831106589094 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 498 0.19563398374430876 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 495 0.19445546576994543 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 483 0.18974139387249223 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 473 0.18581300062461453 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 387 0.1520288186928664 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 376 0.14770758612020096 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 353 0.1386722816500823 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 347 0.1363152457013557 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 344 0.1351367277269924 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 329 0.12924413785517586 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 319 0.12531574460729816 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 312 0.12256586933378377 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 304 0.11942315473548165 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 303 0.11903031541069388 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 291 0.11431624351324066 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 291 0.11431624351324066 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 275 0.10803081431663636 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 264 0.10370958174397091 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 259 0.10174538512003205 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 259 0.10174538512003205 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 255 0.10017402782088099 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.892589871816528E-4 0.0 24-25 0.0 0.0 0.0 0.0015713572991510741 0.0 26-27 0.0 0.0 0.0 0.003928393247877686 0.0 28-29 0.0 0.0 0.0 0.008446045482937025 0.0 30-31 0.0 0.0 0.0 0.0174813499530557 0.0 32-33 0.0 0.0 0.0 0.02533813644881107 0.0 34-35 0.0 0.0 0.0 0.031034306658233715 0.0 36-37 0.0 0.0 0.0 0.03889109315398909 0.0 38-39 0.0 0.0 0.0 0.05205121053437933 0.0 40-41 0.0 0.0 0.0 0.07817502563276595 0.0 42-43 0.0 0.0 0.0 0.12354796764575321 0.0 44-45 0.0 0.0 0.0 0.17029584729549768 0.0 46-47 0.0 0.0 0.0 0.25907753469753336 0.0 48-49 0.0 0.0 0.0 0.3551267496081428 0.0 50-51 0.0 0.0 0.0 0.4213201758348818 0.0 52-53 0.0 0.0 0.0 0.49183483463428623 0.0 54-55 0.0 0.0 0.0 0.549975054702876 0.0 56-57 0.0 0.0 0.0 0.6805941301948091 0.0 58-59 0.0 0.0 0.0 0.8430331909945513 0.0 60-61 0.0 0.0 0.0 1.0253106376960759 0.0 62-63 0.0 0.0 0.0 1.2050346287864802 0.0 64-65 0.0 0.0 0.0 1.3888834327871558 0.0 66-67 0.0 0.0 0.0 1.6137839462281534 0.0 68-69 0.0 0.0 0.0 1.8817003657334115 0.0 70-71 0.0 0.0 0.0 2.1189753179052238 0.0 72-73 0.0 0.0 0.0 2.359589404337732 0.0 74-75 0.0 0.0 0.0 2.6196490373472345 0.0 76-77 0.0 0.0 0.0 2.8979756989593684 0.0 78-79 0.0 0.0 0.0 3.201640497020314 0.0 80-81 0.0 0.0 0.0 3.57110588198321 0.0 82-83 0.0 0.0 0.0 3.923875595642626 0.0 84-85 0.0 0.0 0.0 4.44085214706333 0.0 86-87 0.0 0.0 0.0 4.941329446842947 0.0 88-89 0.0 0.0 0.0 5.4732338926055855 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGCAG 20 0.00206671 71.297745 3 AGTACCC 25 0.004999592 57.049442 5 ATAGGAC 25 0.0050034947 57.038197 3 CGGTATC 55 4.9270602E-5 43.21927 2 TATGGTG 50 0.0016065851 38.03296 5 GCGGTAT 70 2.0313571E-4 33.978096 1 TATACAG 85 1.6175867E-5 33.55849 5 CTACTAG 60 0.003917128 31.712889 1 GTGTAGC 80 4.4457376E-4 29.730831 1 GCTAAAG 65 0.005794153 29.273434 1 CTAGGCC 65 0.0058109327 29.256123 4 TCTGTAC 65 0.005816534 29.250355 3 GTATCAA 8870 0.0 28.906277 1 GGTATCA 6165 0.0 28.472153 1 ATGGGAG 540 0.0 27.292168 5 ATGGGGA 280 0.0 27.166399 5 CTATACA 70 0.008344101 27.166399 4 TCTAGGC 70 0.00835213 27.161045 3 CGGGAGT 90 8.883182E-4 26.411776 4 CATGGGG 605 0.0 25.931562 4 >>END_MODULE