##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937839_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 254557 Sequences flagged as poor quality 0 Sequence length 101 %GC 31 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94782308088169 31.0 31.0 34.0 30.0 34.0 2 32.15407551157501 33.0 31.0 34.0 30.0 34.0 3 32.176117726088854 33.0 31.0 34.0 30.0 34.0 4 35.853875556358695 37.0 35.0 37.0 35.0 37.0 5 35.82092026540225 37.0 35.0 37.0 35.0 37.0 6 35.96095962790259 37.0 35.0 37.0 35.0 37.0 7 35.90391150115691 37.0 35.0 37.0 35.0 37.0 8 35.94201298726808 37.0 35.0 37.0 35.0 37.0 9 37.48906924578778 39.0 37.0 39.0 35.0 39.0 10-11 37.4140605051128 39.0 37.0 39.0 34.0 39.0 12-13 37.28991345749675 39.0 37.0 39.0 34.0 39.0 14-15 38.119226735073084 40.0 37.0 41.0 33.0 41.0 16-17 38.17226790070593 40.0 37.5 41.0 33.5 41.0 18-19 38.35858373566627 40.0 37.5 41.0 34.0 41.0 20-21 38.39647898113193 40.0 37.5 41.0 34.0 41.0 22-23 38.27670227100414 40.0 37.0 41.0 34.0 41.0 24-25 38.150512458899186 40.0 37.0 41.0 34.0 41.0 26-27 37.85353771453938 40.0 36.5 41.0 33.5 41.0 28-29 37.58879150838516 39.0 35.5 41.0 33.0 41.0 30-31 37.28153615889565 39.0 35.0 41.0 33.0 41.0 32-33 36.884037759715895 38.0 35.0 41.0 33.0 41.0 34-35 36.56755461448713 38.0 35.0 40.5 33.0 41.0 36-37 36.229241387979904 38.0 35.0 40.0 31.5 41.0 38-39 35.9024992437843 38.0 35.0 40.0 31.0 41.0 40-41 35.551609266293994 37.5 35.0 40.0 30.5 41.0 42-43 35.130540507626975 37.0 35.0 40.0 29.5 41.0 44-45 34.542267547150544 37.0 34.5 40.0 25.5 41.0 46-47 33.7797840955071 36.5 33.5 40.0 21.5 41.0 48-49 33.1336026901637 36.0 33.0 40.0 15.0 41.0 50-51 32.71226876495244 35.5 33.0 40.0 5.0 41.0 52-53 32.36369261108514 35.0 33.0 40.0 2.0 41.0 54-55 32.14157929265352 35.0 33.0 40.0 2.0 41.0 56-57 31.890503895001906 35.0 33.0 40.0 2.0 41.0 58-59 31.63533511158601 35.0 32.5 39.0 2.0 41.0 60-61 31.238162769045832 35.0 31.5 39.0 2.0 40.5 62-63 30.793778996452662 35.0 31.0 39.0 2.0 40.0 64-65 30.30842404648075 35.0 30.5 38.0 2.0 40.0 66-67 29.871792957962263 35.0 29.5 37.5 2.0 40.0 68-69 29.44017842762132 35.0 29.0 37.0 2.0 40.0 70-71 29.0452590186088 35.0 29.0 36.5 2.0 39.0 72-73 28.535221973860473 34.0 27.5 36.0 2.0 39.0 74-75 28.07887035123764 34.0 26.5 35.0 2.0 38.5 76-77 26.890050165581776 32.5 25.5 35.0 2.0 36.5 78-79 27.332601342724814 33.0 26.0 35.0 2.0 37.0 80-81 27.148852712751957 33.0 26.0 35.0 2.0 37.0 82-83 26.78129652690753 33.0 25.0 35.0 2.0 36.0 84-85 26.427216694099947 33.0 24.0 35.0 2.0 36.0 86-87 26.107594762666125 33.0 24.0 35.0 2.0 35.5 88-89 25.574794643242967 33.0 20.0 35.0 2.0 35.0 90-91 24.97081793075814 33.0 16.5 35.0 2.0 35.0 92-93 24.53731187906834 32.0 8.5 35.0 2.0 35.0 94-95 24.102285539191612 32.0 2.0 35.0 2.0 35.0 96-97 23.507014146144087 31.5 2.0 34.0 2.0 35.0 98-99 22.70593226664362 31.0 2.0 34.0 2.0 35.0 100-101 20.591050334502683 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3353027388147716 1101 2 0.1768882817279689 1101 3 0.3009752079370429 1101 4 0.20362286547187125 1101 5 0.09468306809674232 1101 6 0.16898682574784374 1101 7 0.24908598890398537 1101 8 0.013317953335644006 1101 9 0.2370497133073286 1101 10-11 0.2725843942302859 1101 12-13 0.28853448458708186 1101 14-15 0.11526482277535166 1101 16-17 0.06779425880731793 1101 18-19 0.32787951224296563 1101 20-21 0.3047562853701393 1101 22-23 0.28581006300915135 1101 24-25 0.2157349701905389 1101 26-27 0.3255458302128176 1101 28-29 0.3892328184883169 1101 30-31 0.022467729218170973 1101 32-33 0.13067448363198508 1101 34-35 0.397864963695433 1101 36-37 0.25661815254577647 1101 38-39 0.32733563296558543 1101 40-41 0.5036793553243726 1101 42-43 0.6628376004494427 1101 44-45 0.574244622080677 1101 46-47 -0.045247218386400334 1101 48-49 -0.6515286794156196 1101 50-51 -0.35031099664367815 1101 52-53 0.02746179486737077 1101 54-55 0.2674871864105697 1101 56-57 -0.062162491646940765 1101 58-59 0.12700653747472046 1101 60-61 0.5677784602709188 1101 62-63 0.37289193856430813 1101 64-65 0.2776648389166141 1101 66-67 0.21803104338556878 1101 68-69 0.4039810097846477 1101 70-71 0.23697683721072949 1101 72-73 0.18421682649048776 1101 74-75 -0.22074132665472135 1101 76-77 -0.2910560383413454 1101 78-79 -0.3363819573486708 1101 80-81 -0.3916933151764894 1101 82-83 -0.3972478330472917 1101 84-85 -0.12936736873818333 1101 86-87 -0.25935167550581895 1101 88-89 -0.3083006325059223 1101 90-91 -0.6698246614265209 1101 92-93 -0.6694469735997224 1101 94-95 -0.8553615076295493 1101 96-97 -0.3028836591121866 1101 98-99 -0.24631203767097887 1101 100-101 -0.32084888132022726 1102 1 0.35752846390935034 1102 2 0.2216234645779025 1102 3 0.1820702058353163 1102 4 0.29918822907001896 1102 5 0.2065262795301166 1102 6 0.1506072544994197 1102 7 0.25911120958494394 1102 8 0.28044699915321303 1102 9 0.205135043277906 1102 10-11 0.33002449511300824 1102 12-13 0.3399478935824618 1102 14-15 0.33556221663831565 1102 16-17 0.2978541579245899 1102 18-19 0.27197892376041466 1102 20-21 0.5645608250927197 1102 22-23 0.5323474736859026 1102 24-25 0.47860277178784827 1102 26-27 0.4979081673292427 1102 28-29 0.6251565259284249 1102 30-31 0.5635721848718092 1102 32-33 0.927884445800963 1102 34-35 0.9321966156500352 1102 36-37 0.8871772109736824 1102 38-39 0.8915905720156019 1102 40-41 1.0959450131637993 1102 42-43 1.2593487395835368 1102 44-45 1.078831633429992 1102 46-47 1.2235474432361286 1102 48-49 1.016498853161096 1102 50-51 0.679155165609366 1102 52-53 0.9671843673768379 1102 54-55 0.7682175352966496 1102 56-57 1.037222754254696 1102 58-59 1.0653051923717385 1102 60-61 1.208903933158691 1102 62-63 0.9074495257858288 1102 64-65 1.1505263353436845 1102 66-67 0.8284030484297062 1102 68-69 1.4993151838073473 1102 70-71 1.3604741049702973 1102 72-73 1.3182647457843046 1102 74-75 0.9724228179438512 1102 76-77 0.8062222298385606 1102 78-79 1.039737210368859 1102 80-81 1.2439046251345687 1102 82-83 1.5342412379909582 1102 84-85 1.6276418788448375 1102 86-87 1.2055863236534918 1102 88-89 0.9687853279816814 1102 90-91 1.2208804667786097 1102 92-93 1.125934489199775 1102 94-95 0.6557723721518691 1102 96-97 0.5765089428214019 1102 98-99 0.23519279629287126 1102 100-101 0.544693363320377 1103 1 0.39736362874451103 1103 2 0.3408673159646156 1103 3 0.3753590598384591 1103 4 0.24201920761528584 1103 5 0.2701709681748099 1103 6 0.21372865690653953 1103 7 0.3251760970783977 1103 8 0.13392685263306703 1103 9 0.3825944885944921 1103 10-11 0.34807787030924686 1103 12-13 0.3130967685170418 1103 14-15 0.3526017247492561 1103 16-17 0.3423007827283655 1103 18-19 0.4893716500817149 1103 20-21 0.4611463823925632 1103 22-23 0.26923261235675966 1103 24-25 0.45763861689512453 1103 26-27 -0.01922945695123701 1103 28-29 0.4336010575872322 1103 30-31 0.5178173444741176 1103 32-33 0.18046817159896733 1103 34-35 0.23930022632507786 1103 36-37 0.32752257989047706 1103 38-39 0.27120203198420256 1103 40-41 0.32187384623548354 1103 42-43 0.4117558562763648 1103 44-45 0.671551413649766 1103 46-47 0.5050427336599839 1103 48-49 0.050283520874330634 1103 50-51 -0.4910436831609104 1103 52-53 0.18264747569708817 1103 54-55 -0.06900773942862415 1103 56-57 0.19070914881251966 1103 58-59 0.25103910131993246 1103 60-61 0.43957504775837464 1103 62-63 0.29752671050587054 1103 64-65 0.3575187476932413 1103 66-67 0.24121047909428128 1103 68-69 0.0035014737079208658 1103 70-71 -0.2466687521725639 1103 72-73 0.0313730660354814 1103 74-75 0.19838435640538776 1103 76-77 0.024367180126368737 1103 78-79 -0.04160109418373281 1103 80-81 -0.02987219328510804 1103 82-83 -0.16686420418591297 1103 84-85 0.19835093169674778 1103 86-87 0.009517511513251975 1103 88-89 0.14385727983934515 1103 90-91 -0.3609811240115519 1103 92-93 -0.7606759241963523 1103 94-95 -0.9271357911134324 1103 96-97 -1.1258065987798567 1103 98-99 -0.8257700503842642 1103 100-101 -0.8580408072709353 1104 1 0.3380931120271917 1104 2 0.4701913860181648 1104 3 0.29097637059254566 1104 4 0.30150683245245347 1104 5 0.2544668141727797 1104 6 0.28747304828010556 1104 7 0.11385752709721686 1104 8 0.16888125992790037 1104 9 0.3515480274568503 1104 10-11 0.3135214945674534 1104 12-13 0.4818259623222332 1104 14-15 0.5454299198570851 1104 16-17 0.5825309173353972 1104 18-19 0.5799781054298094 1104 20-21 0.2359328927413813 1104 22-23 0.5491573482085457 1104 24-25 0.4959582000475322 1104 26-27 0.4368061487804198 1104 28-29 0.1232811849573352 1104 30-31 0.41787523997273723 1104 32-33 0.0623975390906466 1104 34-35 -0.06609852892170665 1104 36-37 -0.08704537495102471 1104 38-39 -0.2768150471878883 1104 40-41 -0.11136540691257579 1104 42-43 -0.22523936734499017 1104 44-45 -0.2582864789540835 1104 46-47 -0.5054146407791436 1104 48-49 -0.35151123958304 1104 50-51 -0.5503865692515149 1104 52-53 -0.19743647277591947 1104 54-55 -0.3071029775238614 1104 56-57 -0.09974533085168602 1104 58-59 -0.21302950484594163 1104 60-61 -0.0817739173595875 1104 62-63 -0.6405251059653985 1104 64-65 -0.14110623421441204 1104 66-67 -0.3420143001791338 1104 68-69 -0.49045568362309666 1104 70-71 -0.3983911432566565 1104 72-73 -0.4497385275381198 1104 74-75 -0.42572911877191544 1104 76-77 -0.9559845350277811 1104 78-79 -1.023581120236706 1104 80-81 -0.6933907922140392 1104 82-83 -0.4794998967608137 1104 84-85 -0.5054050387765479 1104 86-87 -0.7562590118620562 1104 88-89 -0.49947026394412575 1104 90-91 -0.1879097678094972 1104 92-93 0.22485020114740095 1104 94-95 -0.07871344345096531 1104 96-97 -0.24843650180489263 1104 98-99 -0.16033366306337626 1104 100-101 -0.23251612931465004 1105 1 0.19231202635451083 1105 2 0.17025610570104277 1105 3 0.014245793239041404 1105 4 -0.054545806222947135 1105 5 0.17692454412990344 1105 6 0.2237646104514539 1105 7 0.1016821367566294 1105 8 0.10812565763035309 1105 9 0.2360773265966145 1105 10-11 0.39034915144527105 1105 12-13 0.44919645554014664 1105 14-15 0.273452194214606 1105 16-17 0.2857709464592517 1105 18-19 0.09459396714542123 1105 20-21 0.0562616147973074 1105 22-23 0.29714834138601276 1105 24-25 0.4549421010264183 1105 26-27 0.28500478427376663 1105 28-29 0.6871446803595447 1105 30-31 0.8648725163527544 1105 32-33 0.513271254809446 1105 34-35 0.7162343459805456 1105 36-37 0.6581141321007209 1105 38-39 0.7540077017806439 1105 40-41 0.940100224239167 1105 42-43 0.7002183895786587 1105 44-45 0.4617469595487407 1105 46-47 0.11184933462737234 1105 48-49 0.19429816095089336 1105 50-51 0.46846978001904205 1105 52-53 0.3880676665871263 1105 54-55 0.19384759281834363 1105 56-57 0.5897417656615396 1105 58-59 0.6289263779035679 1105 60-61 0.6031895429861258 1105 62-63 0.22767595651606243 1105 64-65 0.716840803511765 1105 66-67 0.3791854409421376 1105 68-69 0.7101955581858199 1105 70-71 0.38455522030616507 1105 72-73 0.8080278344067118 1105 74-75 0.9195503893584949 1105 76-77 0.7256552999370314 1105 78-79 0.7773249553235253 1105 80-81 0.3484612693775233 1105 82-83 0.4231037221481202 1105 84-85 1.014658062914254 1105 86-87 0.6061846954971095 1105 88-89 0.7574601354302537 1105 90-91 1.1043198252654953 1105 92-93 1.0816781276905623 1105 94-95 0.40569315795668714 1105 96-97 0.1506412427141548 1105 98-99 -0.2743616543262206 1105 100-101 -0.8447845541993679 1106 1 0.12174251073209419 1106 2 0.026389350865528627 1106 3 0.005091387552148774 1106 4 -0.04543272968137302 1106 5 -0.25570894900945973 1106 6 -0.07723555486512623 1106 7 -0.10919982720435684 1106 8 -0.14945824053463497 1106 9 0.11037473112629215 1106 10-11 -0.11520961859936563 1106 12-13 -0.33410450329602526 1106 14-15 0.2541101542079929 1106 16-17 0.047436097723931425 1106 18-19 0.09562719577824907 1106 20-21 0.10783005586194605 1106 22-23 -0.15229578160952428 1106 24-25 -0.3423281089256349 1106 26-27 0.0021584460360486446 1106 28-29 0.12829197743344167 1106 30-31 -0.02937653307690624 1106 32-33 -0.04034297894820327 1106 34-35 -0.16082177899879468 1106 36-37 0.059905750550051096 1106 38-39 -0.039708201674471866 1106 40-41 -0.04772834246607971 1106 42-43 -0.6360665335491333 1106 44-45 -0.4899392695800415 1106 46-47 -0.21297708296231121 1106 48-49 0.0721705365501677 1106 50-51 0.6489851544610943 1106 52-53 0.6762144677112829 1106 54-55 0.5298535442152641 1106 56-57 0.18939666729817262 1106 58-59 0.33418757810194677 1106 60-61 0.906855438175409 1106 62-63 0.8607661367111383 1106 64-65 1.034444952957955 1106 66-67 0.6553261253527829 1106 68-69 1.1803352506969773 1106 70-71 1.266271206419571 1106 72-73 1.3619520367542073 1106 74-75 0.9746524723474188 1106 76-77 0.826219442770558 1106 78-79 0.5077556050176888 1106 80-81 -0.07577207497691418 1106 82-83 -0.21485296490759254 1106 84-85 -0.7715359360938514 1106 86-87 -0.457596508007601 1106 88-89 -0.18722080356792858 1106 90-91 0.19321881683434938 1106 92-93 0.21080070993555822 1106 94-95 0.02986657505042345 1106 96-97 0.3828077165114756 1106 98-99 0.6044096301836177 1106 100-101 -0.488124362432135 1107 1 0.3577563166253519 1107 2 0.40167268076456253 1107 3 0.18062713863395174 1107 4 0.15843601130358564 1107 5 0.3125993139206855 1107 6 0.22520623371925552 1107 7 0.03918776809751989 1107 8 0.17624235703387825 1107 9 0.15918474555035544 1107 10-11 0.6445523842854541 1107 12-13 0.2964204297350932 1107 14-15 0.734843721073851 1107 16-17 0.5185750839180301 1107 18-19 0.2740220031139202 1107 20-21 0.28644379993777846 1107 22-23 0.24589863421573455 1107 24-25 0.2875786497803148 1107 26-27 0.5990443928730542 1107 28-29 0.44768204543260737 1107 30-31 0.03667796929276079 1107 32-33 0.28431444944660456 1107 34-35 -0.11423976787165913 1107 36-37 -0.02945907533084835 1107 38-39 -0.08361145739259257 1107 40-41 0.030444952403080094 1107 42-43 0.0047613049013151 1107 44-45 0.4435554759381901 1107 46-47 0.7924576996463841 1107 48-49 0.8072313236921502 1107 50-51 0.9671002379146287 1107 52-53 0.7263896171034148 1107 54-55 0.8575889656041014 1107 56-57 0.7057654082831313 1107 58-59 0.9175123256807609 1107 60-61 1.0214495037018914 1107 62-63 0.9697064828887569 1107 64-65 1.3560115097269652 1107 66-67 1.498335604025769 1107 68-69 1.3830191575587136 1107 70-71 1.4489417195042584 1107 72-73 1.684651913119339 1107 74-75 1.4677366470846955 1107 76-77 0.9909500217938394 1107 78-79 0.9697636412839152 1107 80-81 0.6866300474216018 1107 82-83 0.9032183311979125 1107 84-85 1.4007157638855467 1107 86-87 0.8617034095691558 1107 88-89 1.3376864702832982 1107 90-91 0.9330189404692177 1107 92-93 1.0885438585034564 1107 94-95 0.9479327197032141 1107 96-97 1.5093531523243762 1107 98-99 1.8575648949682844 1107 100-101 1.5852967173123567 1108 1 0.1513318827127712 1108 2 0.17952944748388688 1108 3 -0.12498107621596688 1108 4 -0.13927330939151972 1108 5 0.04477414277798175 1108 6 -0.09704231815015163 1108 7 -0.11439306391933002 1108 8 -0.10972394101772664 1108 9 -0.19336923022636654 1108 10-11 0.1490415891300998 1108 12-13 0.08407182520639367 1108 14-15 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1.4997152042943185 2316 56-57 1.5769954815274296 2316 58-59 1.5752119522784938 2316 60-61 1.757563606818362 2316 62-63 1.5936383369746387 2316 64-65 1.7172512770686268 2316 66-67 1.2841140041406618 2316 68-69 1.4618734588656217 2316 70-71 1.5309286504248405 2316 72-73 1.210601575621137 2316 74-75 1.450278295799329 2316 76-77 1.407329455945785 2316 78-79 1.3587931544248022 2316 80-81 1.1934966997266443 2316 82-83 1.5115139652636884 2316 84-85 1.4752526014555585 2316 86-87 1.7003415684087386 2316 88-89 1.4457666799630324 2316 90-91 1.5453268537249976 2316 92-93 1.4923389181042026 2316 94-95 1.4152420691215681 2316 96-97 1.4908446071570651 2316 98-99 1.4001112112112857 2316 100-101 1.0519777456047557 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 5.0 5 25.0 6 61.0 7 97.0 8 167.0 9 316.0 10 461.0 11 780.0 12 1267.0 13 1764.0 14 2117.0 15 3263.0 16 4674.0 17 6588.0 18 5525.0 19 3114.0 20 2466.0 21 2385.0 22 2612.0 23 2936.0 24 3136.0 25 3403.0 26 3806.0 27 4173.0 28 4965.0 29 5867.0 30 6990.0 31 8433.0 32 10599.0 33 13989.0 34 21278.0 35 35290.0 36 30994.0 37 33407.0 38 25354.0 39 2248.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.51626591230551 22.576614804337577 14.21106396353921 12.696055319817695 2 15.04849601464505 28.844227422541906 37.916458789190635 18.19081777362241 3 22.014715761106547 36.93632467384515 28.09586850882121 12.953091056227093 4 10.572877587338002 21.58534237911352 36.40324171010815 31.43853832344033 5 9.694142246258332 49.15183624701295 27.5126556407999 13.641365865928815 6 22.578833031501784 40.54376819337123 18.43909222688828 18.438306548238707 7 24.24329325062756 43.90018738435793 17.405924802696447 14.450594562318066 8 28.234147951146504 33.81560907773112 18.008933166245676 19.94130980487671 9 31.270403092431167 20.996476231256654 19.19216521250643 28.54095546380575 10-11 24.360948628401495 34.450437426588145 21.558432885365555 19.630181059644794 12-13 28.639336855958668 33.66909583767114 23.967078512640203 13.72448879372999 14-15 24.181224637311093 33.5270685936745 28.090368758274174 14.201338010740228 16-17 15.702573490416686 38.205784951896824 27.933429448021464 18.158212109665026 18-19 15.473351744403022 36.07620297222233 34.10356030280055 14.346884980574096 20-21 19.542185050892336 33.366986568823485 33.16487073622018 13.925957644064002 22-23 17.50800416412956 35.47975879475948 32.47097876686767 14.541258274243289 24-25 16.61261841673656 36.69244352432564 33.44169172659115 13.253246332346649 26-27 16.785763391015692 35.85924456404314 34.510812987370116 12.844179057571056 28-29 14.625776848920072 37.93320867085176 34.35106107152117 13.089953408706995 30-31 14.77193712999446 39.58602591953865 32.88006222574904 12.761974724717842 32-33 14.308583146407289 39.888905038950014 32.387048873140394 13.415462941502296 34-35 14.828702412426292 38.89030747533951 32.69169576951331 13.589294342720883 36-37 15.488279638744956 39.338537144922356 32.716837486299724 12.45634573003296 38-39 15.515385552155314 39.64671959521836 32.521203502555416 12.316691350070908 40-41 13.889925556363064 40.673528314116794 32.59256349315472 12.843982636365423 42-43 13.582172526848662 42.01965595208395 30.762370422172765 13.635801098894623 44-45 14.577481664224514 41.530973416562894 29.76582847849401 14.12571644071858 46-47 14.823006242216872 41.8389594471965 28.62502307931033 14.713011231276296 48-49 15.485529763471442 41.98804197095346 28.38224837659149 14.144179888983608 50-51 15.495743585915925 43.03495877151286 28.06012013026552 13.409177512305693 52-53 14.595945112489542 43.71299944609655 27.7203141143241 13.970741327089806 54-55 13.614293122142081 43.301670359370824 27.573304105972753 15.510732412514338 56-57 15.111350306611094 43.763872138656566 26.031890696386274 15.09288685834607 58-59 15.539348750967367 42.82695034903774 26.403713117297894 15.229987782697 60-61 14.956768032307105 42.95108757567068 27.362044650117657 14.730099741904565 62-63 16.148053284726014 42.407987209151585 27.184677694975978 14.259281811146424 64-65 15.425621766441308 43.30267877135573 27.1357691990399 14.135930263163063 66-67 14.903145464473575 42.751918038003275 26.976472852838462 15.368463644684688 68-69 14.55037575081416 43.62244998173297 26.693628538991266 15.133545728461604 70-71 15.32721551558197 43.347462454381535 25.862184108077958 15.46313792195854 72-73 15.122939066692332 42.29642084091186 26.390749419579894 16.189890672815913 74-75 15.00862282317909 43.63619935810054 25.911681863001213 15.443495955719152 76-77 15.219773960252517 42.02673664444506 27.154625486629712 15.598863908672714 78-79 14.387936690014417 41.96722148673971 27.76254434173879 15.882297481507088 80-81 14.129055574979276 43.490652388266675 26.686950270469872 15.693341766284172 82-83 14.006151887993212 42.633448042866796 26.335069689969988 17.025330379170008 84-85 13.948153065914509 41.82658500846569 26.510368993977774 17.71489293164203 86-87 14.3724195366853 42.13201758348818 26.442211371127094 17.05335150869943 88-89 14.641121634840134 41.158365317001696 26.74175135627777 17.458761691880404 90-91 13.71387711885216 41.28110383627895 27.18515069903214 17.819868345836746 92-93 13.245834405463642 42.91559208109823 26.239071636637195 17.599501876800932 94-95 13.044229779577854 42.61128155972925 25.693656037744 18.650832622948887 96-97 12.991982149381082 41.871957950478674 25.614302494136876 19.52175740600337 98-99 14.338327005309253 42.12794459361515 24.76021928420325 18.773509116872354 100-101 14.098302687411598 42.182539682539684 25.114725758290113 18.604431871758603 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7289.0 1 4295.5 2 1473.0 3 1889.5 4 2027.0 5 2184.0 6 3431.0 7 5342.5 8 6091.5 9 5403.0 10 4481.0 11 4087.0 12 4100.5 13 3955.0 14 3604.0 15 3031.5 16 2442.5 17 2062.0 18 1880.0 19 1835.5 20 1814.5 21 1831.0 22 1914.0 23 2118.0 24 2357.5 25 2511.5 26 2792.0 27 3171.0 28 3452.5 29 3687.0 30 3968.5 31 4384.0 32 4944.0 33 5558.0 34 6209.0 35 6685.0 36 7218.0 37 7667.0 38 7284.5 39 6685.0 40 6342.5 41 5992.0 42 5476.0 43 5162.0 44 5158.0 45 4875.5 46 4670.5 47 6092.5 48 13526.5 49 13216.0 50 6405.0 51 4542.0 52 2586.5 53 1904.5 54 1625.0 55 1446.5 56 1417.5 57 1481.5 58 1392.0 59 1405.5 60 1521.5 61 1575.5 62 1354.5 63 703.5 64 265.5 65 157.0 66 127.0 67 115.5 68 90.0 69 65.0 70 58.5 71 49.5 72 45.5 73 43.0 74 39.0 75 31.0 76 16.5 77 13.0 78 14.5 79 14.0 80 11.5 81 7.5 82 3.0 83 1.0 84 1.5 85 1.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014535055017147436 2 0.0 3 0.0 4 0.0 5 0.04910491559847108 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004714071897453222 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.856786495755371E-4 24-25 1.9641966239388427E-4 26-27 7.856786495755371E-4 28-29 0.0011785179743633056 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.856786495755371E-4 42-43 0.011195920756451405 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 3.9283932478776854E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.9283932478776854E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.010410242106875866 92-93 5.892589871816528E-4 94-95 0.0 96-97 0.0 98-99 5.892589871816528E-4 100-101 0.014535055017147436 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 254557.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.115011963896755 #Duplication Level Percentage of deduplicated Percentage of total 1 76.69952527742137 32.30201424683802 2 12.705181140298253 10.701577114542726 3 4.110803128339624 5.193795687937424 4 1.895999963895844 3.1940024465208516 5 1.0369390883407668 2.1835351055651793 6 0.6503681912999684 1.6434158494521636 7 0.4929938777944411 1.453371014300852 8 0.34360045087031443 1.157658967936289 9 0.23703435325529307 0.8984434160881077 >10 1.5915161514682425 12.466634672920778 >50 0.1254887574008608 3.615164659968536 >100 0.08863888419584612 7.151893794770102 >500 0.01195131022865341 3.5581206416652855 >1k 0.007967540152435606 8.175750035051248 >5k 0.0019918850381089015 6.304622346442403 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9465 3.718224209116229 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5566 2.1865436817687196 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4627 1.8176675557930053 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3693 1.4507556264412291 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2909 1.1427695958076187 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2381 0.935350432319677 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2359 0.926707967174346 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1216 0.4776926189419266 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1198 0.4706215110957467 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1109 0.4356588111896353 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 924 0.3629835361038981 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 885 0.3476628024371752 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 839 0.3295921934969378 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 831 0.3264494788986357 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 819 0.3217354070011824 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 753 0.29580801156518977 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 681 0.2675235801804704 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 668 0.2624166689582294 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 559 0.21959718255636262 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 521 0.2046692882144274 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 502 0.19720534104345983 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 501 0.19681250171867207 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 496 0.1948483050947332 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 445 0.174813499530557 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 432 0.16970658830831603 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 403 0.15831424788947074 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 403 0.15831424788947074 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 393 0.15438585464159305 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 384 0.1508503007185031 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 372 0.1461362288210499 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 339 0.13317253110305355 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 335 0.13160117380390246 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 325 0.12767278055602477 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 319 0.12531574460729816 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 316 0.12413722663293487 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 303 0.11903031541069388 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 283 0.1111735289149385 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 267 0.10488809971833421 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 263 0.10331674241918314 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.892589871816528E-4 0.0 24-25 0.0 0.0 0.0 0.0015713572991510741 0.0 26-27 0.0 0.0 0.0 0.003928393247877686 0.0 28-29 0.0 0.0 0.0 0.008642465145330909 0.0 30-31 0.0 0.0 0.0 0.01787418927784347 0.0 32-33 0.0 0.0 0.0 0.025927395435992723 0.0 34-35 0.0 0.0 0.0 0.03221282463259702 0.0 36-37 0.0 0.0 0.0 0.039676771803564624 0.0 38-39 0.0 0.0 0.0 0.052444049859167105 0.0 40-41 0.0 0.0 0.0 0.07778218630797817 0.0 42-43 0.0 0.0 0.0 0.12433364629532875 0.0 44-45 0.0 0.0 0.0 0.17304572256901204 0.0 46-47 0.0 0.0 0.0 0.26457728524456214 0.0 48-49 0.0 0.0 0.0 0.3647513130654431 0.0 50-51 0.0 0.0 0.0 0.4334981949033026 0.0 52-53 0.0 0.0 0.0 0.5065663093138275 0.0 54-55 0.0 0.0 0.0 0.5645101097200234 0.0 56-57 0.0 0.0 0.0 0.6955220245367442 0.0 58-59 0.0 0.0 0.0 0.8565861476997293 0.0 60-61 0.0 0.0 0.0 1.0410242106875867 0.0 62-63 0.0 0.0 0.0 1.2217303000899602 0.0 64-65 0.0 0.0 0.0 1.407150461389787 0.0 66-67 0.0 0.0 0.0 1.6351936894290866 0.0 68-69 0.0 0.0 0.0 1.9066456628574346 0.0 70-71 0.0 0.0 0.0 2.1478490082771247 0.0 72-73 0.0 0.0 0.0 2.389641612683996 0.0 74-75 0.0 0.0 0.0 2.652647540629407 0.0 76-77 0.0 0.0 0.0 2.939616667386872 0.0 78-79 0.0 0.0 0.0 3.2477991176828764 0.0 80-81 0.0 0.0 0.0 3.619621538594499 0.0 82-83 0.0 0.0 0.0 3.9788731011129137 0.0 84-85 0.0 0.0 0.0 4.498599527807132 0.0 86-87 0.0 0.0 0.0 5.00771929273208 0.0 88-89 0.0 0.0 0.0 5.545123489041747 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGTGCG 20 0.002069413 71.27432 2 CGCCCAC 20 5.386708E-4 47.497543 92-93 TCTGCAT 55 0.0025740415 34.550453 8 GGGTCTG 55 0.0025740415 34.550453 7 ATGGGCG 60 0.003938638 31.677475 5 GTACTAT 65 0.0058259135 29.240746 1 GAGAGCT 65 0.005831512 29.235 9 ATGGGGT 245 0.0 29.091558 5 GGTCGGG 85 6.3806283E-4 27.950712 1 GTCGGGA 85 6.3806283E-4 27.950712 2 ACATGGA 205 3.092282E-11 27.814367 3 ATGGGAG 995 0.0 27.697893 5 AGCCCTG 70 0.008365576 27.15212 5 TAGATCA 70 0.008365576 27.15212 4 GTATAGC 105 6.869099E-5 27.15212 1 TCCGCGC 35 0.008389373 27.141453 88-89 GGGAGAG 200 7.1122486E-10 26.128782 7 TAGCCCT 95 0.0012214986 25.008533 4 GGGAATG 210 1.2514647E-9 24.884554 7 GAGTACT 3455 0.0 24.406572 12-13 >>END_MODULE