##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937838_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 242507 Sequences flagged as poor quality 0 Sequence length 101 %GC 31 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01700981827329 31.0 31.0 34.0 30.0 34.0 2 32.142119608918506 33.0 31.0 34.0 30.0 34.0 3 32.162543761623375 33.0 31.0 34.0 30.0 34.0 4 35.840099461046485 37.0 35.0 37.0 35.0 37.0 5 35.81903615153377 37.0 35.0 37.0 35.0 37.0 6 35.94978701645726 37.0 35.0 37.0 35.0 37.0 7 35.89216393753583 37.0 35.0 37.0 35.0 37.0 8 35.92738353944422 37.0 35.0 37.0 35.0 37.0 9 37.44286969035945 39.0 37.0 39.0 35.0 39.0 10-11 37.399553414952976 39.0 37.0 39.0 34.0 39.0 12-13 37.28601030073359 39.0 37.0 39.0 34.0 39.0 14-15 38.12953440519243 40.0 37.0 41.0 33.0 41.0 16-17 38.187066765083074 40.0 37.5 41.0 33.5 41.0 18-19 38.35967003014346 40.0 37.5 41.0 34.0 41.0 20-21 38.38855785606189 40.0 37.5 41.0 34.0 41.0 22-23 38.211793886362045 40.0 37.0 41.0 34.0 41.0 24-25 38.114722461619664 40.0 37.0 41.0 34.0 41.0 26-27 37.81768979864499 40.0 36.0 41.0 33.0 41.0 28-29 37.557076702940535 39.0 35.5 41.0 33.0 41.0 30-31 37.2485412792208 39.0 35.0 41.0 33.0 41.0 32-33 36.85849068274318 38.0 35.0 41.0 33.0 41.0 34-35 36.54750790698826 38.0 35.0 40.5 33.0 41.0 36-37 36.19468097828104 38.0 35.0 40.0 31.5 41.0 38-39 35.87282841320044 38.0 35.0 40.0 31.0 41.0 40-41 35.49294453355986 37.0 35.0 40.0 30.5 41.0 42-43 35.082040930777254 37.0 35.0 40.0 29.5 41.0 44-45 34.476155327475084 37.0 34.5 40.0 25.5 41.0 46-47 33.7210389803181 36.0 33.0 40.0 21.5 41.0 48-49 33.07715653568763 36.0 33.0 40.0 13.5 41.0 50-51 32.67154762542937 35.5 33.0 40.0 4.5 41.0 52-53 32.30903644018523 35.0 33.0 40.0 2.0 41.0 54-55 32.0801605726845 35.0 33.0 40.0 2.0 41.0 56-57 31.82772456052815 35.0 33.0 40.0 2.0 41.0 58-59 31.595281785680413 35.0 33.0 39.0 2.0 41.0 60-61 31.175005257580196 35.0 31.5 39.0 2.0 40.5 62-63 30.752951461194936 35.0 31.0 39.0 2.0 40.0 64-65 30.299484138602185 35.0 30.5 38.0 2.0 40.0 66-67 29.852767136618738 35.0 29.5 38.0 2.0 40.0 68-69 29.42618151228624 35.0 29.0 37.0 2.0 40.0 70-71 28.989237424074357 35.0 29.0 36.5 2.0 39.0 72-73 28.484629309669412 34.0 27.5 36.0 2.0 39.0 74-75 27.995509407975852 34.0 26.5 35.0 2.0 38.5 76-77 26.83293265761401 32.5 25.5 35.0 2.0 36.5 78-79 27.2534813428066 33.0 26.0 35.0 2.0 37.0 80-81 27.042889483602536 33.0 25.5 35.0 2.0 37.0 82-83 26.695258281204254 33.0 24.5 35.0 2.0 36.0 84-85 26.30204901301818 33.0 24.0 35.0 2.0 36.0 86-87 25.84102933111209 33.0 21.0 35.0 2.0 35.0 88-89 25.49223321388661 33.0 19.5 35.0 2.0 35.0 90-91 24.943376892213422 33.0 15.0 35.0 2.0 35.0 92-93 24.509880127171588 32.0 4.5 35.0 2.0 35.0 94-95 24.07796475978013 32.0 2.0 35.0 2.0 35.0 96-97 23.535322691716118 32.0 2.0 34.5 2.0 35.0 98-99 22.750153603813498 31.0 2.0 34.0 2.0 35.0 100-101 20.74315380586952 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4420013435769583 1101 2 0.23998456997537687 1101 3 0.3953106554306345 1101 4 0.3259430962475065 1101 5 0.27576758053747596 1101 6 0.40457099918294404 1101 7 0.417321619573201 1101 8 0.14603302883696756 1101 9 0.41518222347557554 1101 10-11 0.4728007706515598 1101 12-13 0.5892191869430476 1101 14-15 0.5712730131922612 1101 16-17 0.486579309362547 1101 18-19 0.648624478556421 1101 20-21 0.6722277606524614 1101 22-23 0.6972209627063677 1101 24-25 0.8188741617173818 1101 26-27 0.7041290719456086 1101 28-29 0.41360522228788454 1101 30-31 0.5539396004763049 1101 32-33 0.6791372835302028 1101 34-35 0.5892431925396195 1101 36-37 0.7545040074306613 1101 38-39 0.4508743010696037 1101 40-41 0.3598510874265557 1101 42-43 -0.008532581635193992 1101 44-45 0.36417535322399885 1101 46-47 0.030360606431969472 1101 48-49 0.010772631309734493 1101 50-51 -0.29187251770582634 1101 52-53 -0.17322894071264727 1101 54-55 0.16835229254996165 1101 56-57 -0.1616848307510672 1101 58-59 0.035931450957960465 1101 60-61 0.20350369945365543 1101 62-63 0.28425670595207464 1101 64-65 0.313792030077348 1101 66-67 0.6515572194214059 1101 68-69 0.8177402055853484 1101 70-71 0.43176876847379475 1101 72-73 0.6676194040859862 1101 74-75 0.5830148109145163 1101 76-77 0.6508964588080595 1101 78-79 0.6538717062610466 1101 80-81 0.7896568314817074 1101 82-83 0.8906789740966197 1101 84-85 0.6587552233349037 1101 86-87 0.2648906420362529 1101 88-89 0.4739800803069123 1101 90-91 0.4778534831869301 1101 92-93 0.21141838233620547 1101 94-95 -0.19272840659943213 1101 96-97 -0.07018193260552152 1101 98-99 0.38137361363135014 1101 100-101 0.6242526805120931 1102 1 0.12324930042560212 1102 2 0.2665864006389924 1102 3 0.14657740927176377 1102 4 0.2108523803272675 1102 5 -0.05005376630789726 1102 6 0.04138041402439541 1102 7 0.05484613738948241 1102 8 -0.22310312676943767 1102 9 -0.06076988505662939 1102 10-11 0.0383450661729654 1102 12-13 0.03671019707707046 1102 14-15 0.07982360161985724 1102 16-17 0.09727724986598218 1102 18-19 0.23430403069764338 1102 20-21 0.3632011179587664 1102 22-23 0.12077645469722142 1102 24-25 0.1449406867255547 1102 26-27 -0.15681404672061916 1102 28-29 -0.12992411736559006 1102 30-31 -0.09455664012519804 1102 32-33 -0.1311969760317382 1102 34-35 -0.054206464210949434 1102 36-37 0.03596853832310387 1102 38-39 -0.24962014155870804 1102 40-41 -0.33989760495656896 1102 42-43 -0.3383176421124787 1102 44-45 -0.23508093968217736 1102 46-47 -0.15922340795181356 1102 48-49 -0.5881158943522493 1102 50-51 -0.7982675814521514 1102 52-53 -0.7944695422164045 1102 54-55 -1.0959395511898222 1102 56-57 -1.2197818395774789 1102 58-59 -1.1153945542725054 1102 60-61 -1.1090882423573944 1102 62-63 -1.212842019121478 1102 64-65 -0.9554033769184045 1102 66-67 -0.8332213029715341 1102 68-69 -0.9700478787559419 1102 70-71 -0.6515509438504985 1102 72-73 -0.25817333864039327 1102 74-75 -0.6949661469148403 1102 76-77 -0.9946269965695187 1102 78-79 -0.8715144493453622 1102 80-81 -1.0188385918592537 1102 82-83 -1.1402134744159689 1102 84-85 -1.1953842013136722 1102 86-87 -1.3008988316479595 1102 88-89 -1.0990727964501978 1102 90-91 -0.9879339473459225 1102 92-93 -1.4019867337147893 1102 94-95 -1.0805593971192096 1102 96-97 -1.0604054533639378 1102 98-99 -1.4682606622758136 1102 100-101 -1.1461584015408661 1103 1 0.08799544609280119 1103 2 0.06848698543431908 1103 3 -0.07052785388613358 1103 4 0.05589269576232425 1103 5 0.014218340727367718 1103 6 0.1142140131737861 1103 7 0.10646983046054004 1103 8 0.0587837241963598 1103 9 0.16897271639650313 1103 10-11 -0.08534800488852312 1103 12-13 0.08883229525179814 1103 14-15 -0.0428338943929063 1103 16-17 -0.052177827220674544 1103 18-19 -0.2032901124886024 1103 20-21 -0.16421116274298697 1103 22-23 0.08676861312358852 1103 24-25 0.2454745339699258 1103 26-27 -0.13933664108531474 1103 28-29 -0.1281686238337798 1103 30-31 0.0787111252478283 1103 32-33 0.4152876066370581 1103 34-35 0.493429988030762 1103 36-37 0.5438101119541585 1103 38-39 0.22841680687297838 1103 40-41 0.13237445777601664 1103 42-43 0.59568244649288 1103 44-45 0.6777092428448412 1103 46-47 0.38289736114263917 1103 48-49 0.18762396033498163 1103 50-51 0.46175589896606795 1103 52-53 -0.1618051792888835 1103 54-55 0.2548434986063839 1103 56-57 0.1031791297650706 1103 58-59 0.48980768744289094 1103 60-61 0.20084774027595742 1103 62-63 0.43727759810340316 1103 64-65 0.6489238869483351 1103 66-67 0.9104766413843031 1103 68-69 1.0914608488712076 1103 70-71 0.44416498739175125 1103 72-73 0.2796611465625105 1103 74-75 0.3897571828742805 1103 76-77 0.34515791393907946 1103 78-79 0.7610668460648817 1103 80-81 0.768320277536457 1103 82-83 1.1666268587401554 1103 84-85 0.800551028174187 1103 86-87 0.8339142110598203 1103 88-89 0.4232502143596548 1103 90-91 0.41182571063741946 1103 92-93 0.18310209315034953 1103 94-95 0.40444414709486054 1103 96-97 0.4330543641090365 1103 98-99 0.008530273396704757 1103 100-101 -0.31469685626353083 1104 1 0.36648904348403377 1104 2 0.4416278428271383 1104 3 0.19718868154694036 1104 4 0.24957492487762067 1104 5 0.1639174313713383 1104 6 0.2672403394284615 1104 7 0.3155952049402444 1104 8 0.36239728765244195 1104 9 0.4429858132442419 1104 10-11 0.4211672791858163 1104 12-13 0.5097209720459901 1104 14-15 0.14339850837492918 1104 16-17 0.44969125732556847 1104 18-19 0.3297635209587355 1104 20-21 0.38959461153523023 1104 22-23 0.2529381828364663 1104 24-25 0.27763339290043376 1104 26-27 0.3704859947215695 1104 28-29 0.18507536460705865 1104 30-31 0.6487090043008266 1104 32-33 1.0824116025012032 1104 34-35 1.0084489439946012 1104 36-37 0.9667600841167285 1104 38-39 0.9918583085034598 1104 40-41 1.0907773846828803 1104 42-43 1.2794558763293011 1104 44-45 1.120337626418106 1104 46-47 1.431042858107034 1104 48-49 2.0422197873717494 1104 50-51 2.035730553649387 1104 52-53 2.1512929940455123 1104 54-55 2.1940651110563465 1104 56-57 2.0614163576873388 1104 58-59 2.4792360922256265 1104 60-61 2.4314344472406155 1104 62-63 2.4625102394777763 1104 64-65 1.7143153342295427 1104 66-67 1.924885825084825 1104 68-69 1.8555452625619218 1104 70-71 1.9614308215867169 1104 72-73 2.3353824011026596 1104 74-75 1.7708051958120556 1104 76-77 1.574723915158625 1104 78-79 2.3446454138954174 1104 80-81 2.3902217065245033 1104 82-83 2.6486385769723455 1104 84-85 2.4346147626316217 1104 86-87 2.530638673532316 1104 88-89 2.324997344453241 1104 90-91 2.0809248383979657 1104 92-93 1.934666291564941 1104 94-95 2.0841214980320544 1104 96-97 2.586882673449157 1104 98-99 2.2268154877449113 1104 100-101 2.724878689217526 1105 1 0.15559140568875662 1105 2 -0.046624125676796524 1105 3 -0.04252785388613489 1105 4 -0.05385814598852079 1105 5 -0.22102745051842732 1105 6 -0.14598800702823667 1105 7 0.15424087423158284 1105 8 0.054554767967402995 1105 9 0.27215116757495394 1105 10-11 0.16360822406770836 1105 12-13 0.1398154602349706 1105 14-15 0.038863075304057304 1105 16-17 0.019014091971243374 1105 18-19 0.001369820171333913 1105 20-21 0.08112217059035487 1105 22-23 0.14739487574985333 1105 24-25 0.25886173935712975 1105 26-27 0.20850174275306443 1105 28-29 0.12187851421334983 1105 30-31 0.091653886190592 1105 32-33 0.14767144502089025 1105 34-35 -0.25449593789516456 1105 36-37 -0.23845251430847014 1105 38-39 -0.3763306678744911 1105 40-41 -0.16572655232499045 1105 42-43 -0.32260711579668566 1105 44-45 -0.2626072554716572 1105 46-47 0.08822396046923586 1105 48-49 0.17577884248985498 1105 50-51 0.12302189223206028 1105 52-53 0.2510567735730618 1105 54-55 -0.04316323540034972 1105 56-57 0.13773131831726104 1105 58-59 0.6358817615169698 1105 60-61 0.4690564944847111 1105 62-63 0.2523685071943085 1105 64-65 0.2584255704500187 1105 66-67 0.4315944865021528 1105 68-69 0.1199389633493233 1105 70-71 0.11850168772845038 1105 72-73 0.5385635034648679 1105 74-75 0.4009154320325301 1105 76-77 0.15707037185153538 1105 78-79 -0.01160655460851956 1105 80-81 0.11513509235127373 1105 82-83 0.6504181045314006 1105 84-85 0.6182210618442205 1105 86-87 0.5288906420362522 1105 88-89 0.4046508877603294 1105 90-91 0.3262634210751294 1105 92-93 0.8738158978641586 1105 94-95 0.720861655512369 1105 96-97 0.6593708624255363 1105 98-99 0.5927524956189245 1105 100-101 0.29097317740650297 1106 1 0.09044854854590412 1106 2 0.27642349337082095 1106 3 0.03423405087577436 1106 4 -1.4386027423540781E-4 1106 5 -0.04369411718509042 1106 6 -0.0059880070282360975 1106 7 0.11690754089825361 1106 8 0.06922143463407338 1106 9 0.029674977098764543 1106 10-11 0.2602748907343724 1106 12-13 0.3395297459492568 1106 14-15 0.41257736101834297 1106 16-17 0.45510933006647747 1106 18-19 0.19127458207609038 1106 20-21 0.2905507420189153 1106 22-23 0.3612996376546107 1106 24-25 0.16514745364283812 1106 26-27 0.20164459989592132 1106 28-29 0.3574975618324032 1106 30-31 0.2301300766667822 1106 32-33 0.32033811168755477 1106 34-35 0.38893263353340757 1106 36-37 -0.11340489526085662 1106 38-39 -0.2891878107316401 1106 40-41 -0.09677417137260846 1106 42-43 -0.39755949674906077 1106 44-45 -0.40155963642403947 1106 46-47 0.0012715795168602995 1106 48-49 0.1912074139184341 1106 50-51 0.2669266541368245 1106 52-53 0.04343772595402129 1106 54-55 0.15893200269488972 1106 56-57 -0.11541153882560096 1106 58-59 -0.03135633372112778 1106 60-61 0.28362792305614093 1106 62-63 0.27017803100383375 1106 64-65 0.1895684275928744 1106 66-67 0.2603563912640574 1106 68-69 0.2249865823969408 1106 70-71 0.3659302591570217 1106 72-73 -0.06581744891608565 1106 74-75 0.40796305108014863 1106 76-77 -0.00407248529132076 1106 78-79 0.21515535015338827 1106 80-81 0.9331350923512751 1106 82-83 1.0243228664361617 1106 84-85 0.919935347558507 1106 86-87 1.5339382610838719 1106 88-89 1.1942699353793778 1106 90-91 1.5555967544084623 1106 92-93 1.3401968502451105 1106 94-95 1.3461949888457028 1106 96-97 1.1385137195683903 1106 98-99 1.383133447999878 1106 100-101 1.2856398440731702 1107 1 0.10894109894029569 1107 2 0.319339064507254 1107 3 -0.1134726391622074 1107 4 -0.3257354466020175 1107 5 -0.2984998431564634 1107 6 -0.059362240157071255 1107 7 -0.07061802147393337 1107 8 0.028935136065562972 1107 9 0.31953767064243976 1107 10-11 0.057718653515557605 1107 12-13 0.32436760747422966 1107 14-15 0.7274765722365757 1107 16-17 0.4160938465724726 1107 18-19 0.19256000422041097 1107 20-21 0.15378474727746294 1107 22-23 0.40154211501365467 1107 24-25 0.2514752362896431 1107 26-27 0.10032996361196211 1107 28-29 0.15098280869187874 1107 30-31 0.25069683097586903 1107 32-33 0.10643218121720821 1107 34-35 0.7484856571968592 1107 36-37 0.3561977924399926 1107 38-39 0.5328104364199859 1107 40-41 0.8185924660799131 1107 42-43 0.6622272400315339 1107 44-45 0.03828845004981929 1107 46-47 0.123328254947765 1107 48-49 0.04523896518924886 1107 50-51 -0.33945663537529924 1107 52-53 -0.31630518961711473 1107 54-55 -0.5987951372408418 1107 56-57 -0.806428190885196 1107 58-59 -0.8287562752928466 1107 60-61 -0.5050662049017909 1107 62-63 -0.2916683026216411 1107 64-65 -0.13076461359906588 1107 66-67 0.31080921042853404 1107 68-69 -0.08853956425803844 1107 70-71 -0.3554983122715498 1107 72-73 -0.02908066831427547 1107 74-75 0.40885408233927834 1107 76-77 0.3092053411766891 1107 78-79 0.34198853741602164 1107 80-81 0.17822711689115067 1107 82-83 0.13327700023692302 1107 84-85 0.499865233623364 1107 86-87 0.9059949365147801 1107 88-89 0.6027858570854825 1107 90-91 1.4934290652469073 1107 92-93 1.2025398242445249 1107 94-95 1.2288125757577681 1107 96-97 1.6951745434071306 1107 98-99 1.6816727410176995 1107 100-101 2.096273790903439 1108 1 0.20053258215935088 1108 2 0.18090528608791345 1108 3 0.24711920493739825 1108 4 0.10825950107030025 1108 5 0.07003137301098405 1108 6 -0.0765762423223535 1108 7 0.10906440364335168 1108 8 -0.026856996738480632 1108 9 -0.25043706771916163 1108 10-11 -0.04168589357935559 1108 12-13 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-0.6504815407167719 2316 78-79 -1.068568303242401 2316 80-81 -1.1682310278673036 2316 82-83 -0.7405873599494761 2316 84-85 -0.4362707414344662 2316 86-87 -0.128551980914569 2316 88-89 0.1485634560663378 2316 90-91 -0.8121628084330688 2316 92-93 -0.528577544758793 2316 94-95 -0.7128214045969195 2316 96-97 -0.7790116512356668 2316 98-99 -1.3894660836160462 2316 100-101 -0.5460541449978678 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 11.0 5 21.0 6 45.0 7 74.0 8 165.0 9 280.0 10 474.0 11 754.0 12 1219.0 13 1747.0 14 2048.0 15 3150.0 16 4488.0 17 6280.0 18 5428.0 19 2902.0 20 2480.0 21 2379.0 22 2545.0 23 2799.0 24 3006.0 25 3301.0 26 3636.0 27 4057.0 28 4759.0 29 5559.0 30 6635.0 31 8018.0 32 10087.0 33 13037.0 34 19654.0 35 33413.0 36 29757.0 37 31640.0 38 24332.0 39 2325.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.75910367611577 22.501309510552385 14.182308615548326 12.557278197783525 2 14.954207507412157 28.864733801498517 38.00055256136936 18.180506129719966 3 22.09626938603834 37.097073486538534 28.11588943824302 12.690767689180108 4 10.347742539390616 21.599376512842927 36.50739978639792 31.54548116136854 5 9.736630437024358 49.10924577721863 27.46550621816157 13.688617567595438 6 22.599347647696767 40.56089102582606 18.400293599772375 18.439467726704798 7 24.39228558350893 44.01893553588144 17.258470889500096 14.330307991109533 8 28.27093650904922 33.8901557480815 17.8572164927198 19.98169125014948 9 31.43043293595649 20.91898378191145 19.253877207668232 28.39670607446383 10-11 24.28321656694446 34.493024943609875 21.488658059354986 19.735100430090675 12-13 28.6415924550555 33.85080424836129 23.904558266510236 13.603045030072971 14-15 24.334761470802903 33.53181557645758 28.001047392446406 14.132375560293106 16-17 15.776451813762076 38.454147715323685 27.61322353581546 18.15617693509878 18-19 15.516046959469213 36.25915952941565 34.00541015310898 14.21938335800616 20-21 19.766027372405745 33.51635210530005 32.92709076439031 13.790529757903897 22-23 17.64741054749838 35.788010869968616 32.25217832356713 14.312400258965868 24-25 16.645120852430757 36.85076482486036 33.232717473552256 13.27139684915662 26-27 16.753744268135783 36.13775442879293 34.320258634909116 12.788242668162175 28-29 14.615283109899671 38.29540253110271 34.15359809981732 12.9357162591803 30-31 14.812768291224584 39.83596349796088 32.761116173966116 12.590152036848421 32-33 14.302061383795106 40.1522430280363 32.20711154729554 13.338584040873048 34-35 14.85503511238851 39.28670100244529 32.36463277348695 13.49363111167925 36-37 15.424090850985744 39.775965229869655 32.449784954661105 12.3501589644835 38-39 15.59442736586442 39.90264178485938 32.27171230461864 12.231218544657565 40-41 13.745902146347497 41.19502690666172 32.33273540751737 12.726335539473412 42-43 13.568434011717228 42.447832354148154 30.39137849614478 13.592355137989841 44-45 14.546384228084138 41.88538887537267 29.47749961856771 14.090727277975482 46-47 14.78101663044778 42.25630600353804 28.285369082129584 14.677308283884589 48-49 15.46573913330337 42.42702272511721 28.031561975530607 14.075676166048815 50-51 15.456667230224284 43.37008828611133 27.749920620848055 13.42332386281633 52-53 14.605557777713631 44.07934616320354 27.356117555369536 13.958978503713295 54-55 13.58476250170098 43.57647408116054 27.27137773342625 15.567385683712223 56-57 14.920187870865584 44.16738485899376 25.768946875760285 15.143480394380369 58-59 15.393988627132412 43.11875533489755 26.15017298469735 15.337083053272691 60-61 14.860189602774355 43.30740968301946 27.110145274157034 14.722255440049153 62-63 16.2172638315595 42.70330340979848 26.861698837559327 14.21773392108269 64-65 15.415225127522092 43.56266004692648 26.92107856680426 14.101036258747172 66-67 14.851942418157002 42.86824710214551 26.76809329215239 15.511717187545102 68-69 14.567856944040674 43.84294853952368 26.356613121710136 15.232581394725504 70-71 15.205334279010502 43.69626443772757 25.489161137616644 15.60924014564528 72-73 14.934208084715081 42.62000684516323 26.008527588894342 16.437257481227345 74-75 14.861014321236087 43.86739351853761 25.734721059598282 15.536871100628021 76-77 15.120388277451784 42.136928006201885 26.962520669506446 15.780163046839885 78-79 14.27237151917264 42.234657143917495 27.41467256615273 16.078298770757133 80-81 14.059181796814112 43.70141892811342 26.42562895091688 15.813770324155591 82-83 13.960206185567008 42.80041237113402 26.118762886597942 17.120618556701032 84-85 13.937180935729426 42.10699941444748 26.174610112739476 17.78120953708362 86-87 14.361441113040035 42.516092318984605 25.963374253114342 17.159092314861013 88-89 14.59669205424998 41.3777746621747 26.420474460531036 17.605058823044285 90-91 13.619079826457051 41.53875022682657 26.943697520579356 17.89847242613702 92-93 13.08780806637512 43.068983362920896 26.03004942257851 17.813159148125468 94-95 12.918802343849869 42.77051796442989 25.428338151063677 18.882341540656558 96-97 12.999624753099912 42.147030807358135 25.290403988338483 19.56294045120347 98-99 14.130073958610396 42.53434529000971 24.537887550747318 18.797693200632576 100-101 13.97434575488235 42.418593140995235 24.682518405477303 18.92454269864511 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6885.0 1 4023.5 2 1368.5 3 1777.0 4 1878.5 5 2086.5 6 3206.0 7 4996.0 8 5758.0 9 5073.0 10 4278.0 11 3968.0 12 3979.0 13 3865.0 14 3496.5 15 2903.5 16 2368.0 17 2014.0 18 1831.0 19 1780.0 20 1731.0 21 1795.0 22 1917.0 23 2015.0 24 2176.5 25 2377.5 26 2713.0 27 3052.5 28 3255.0 29 3592.0 30 3905.5 31 4166.0 32 4802.5 33 5434.5 34 5913.0 35 6401.0 36 6847.5 37 7225.0 38 7029.5 39 6385.5 40 5924.5 41 5604.0 42 5230.5 43 4982.5 44 4885.0 45 4582.5 46 4463.5 47 5681.0 48 12620.0 49 12446.5 50 6114.5 51 4397.0 52 2438.5 53 1800.5 54 1551.5 55 1357.5 56 1368.5 57 1395.0 58 1320.0 59 1356.5 60 1442.5 61 1532.0 62 1339.0 63 721.5 64 290.0 65 167.5 66 127.0 67 102.5 68 78.5 69 61.5 70 50.5 71 42.5 72 39.5 73 38.5 74 34.5 75 29.0 76 21.5 77 13.0 78 9.5 79 8.5 80 5.5 81 2.0 82 1.5 83 1.0 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020617961543378128 2 0.0 3 0.0 4 0.0 5 0.029689864622464507 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005566849616712095 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0016494369234702504 24-25 2.061796154337813E-4 26-27 0.0012370776926026877 28-29 0.0016494369234702504 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.061796154337813E-4 40-41 8.247184617351252E-4 42-43 0.017937626542738973 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.061796154337813E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.002886514616072938 84-85 4.123592308675626E-4 86-87 0.0 88-89 0.0 90-91 0.012783136156894439 92-93 0.0026803350006391565 94-95 0.0 96-97 0.0 98-99 0.0026803350006391565 100-101 0.021442680005113252 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 242507.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.58183559448883 #Duplication Level Percentage of deduplicated Percentage of total 1 77.62385291798452 33.82989996082383 2 12.285564914551175 10.70854940582779 3 4.012629740684356 5.24633308980186 4 1.7863021080999284 3.114012991891996 5 0.9916444886602482 2.1608843536485947 6 0.6135170140137889 1.6042918583502388 7 0.40302350610686094 1.2295152928704578 8 0.3142383785531228 1.0956068281264755 9 0.21248497601238417 0.8334436761783568 >10 1.5332835806146987 12.372095092800537 >50 0.125695335779392 3.860709294866032 >100 0.0788089803696188 7.010143769191429 >500 0.00897823826995657 2.905096791474642 >1k 0.007980656239961395 7.857499897084887 >5k 0.001995164059990349 6.171917697062859 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8918 3.677419620876923 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5278 2.176432020518995 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4307 1.776031207346592 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3329 1.3727438795581157 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2801 1.1550182056600429 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2192 0.9038914340616973 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2150 0.8865723463652596 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1146 0.4725636785742267 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1085 0.4474097654913054 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1063 0.438337862412219 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 908 0.3744221816277468 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 854 0.3521547831608985 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 844 0.34803119085222284 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 805 0.33194918084838787 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 784 0.32328963700016905 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 709 0.2923626946851019 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 703 0.2898885392998965 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 573 0.23628183928711335 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 502 0.2070043338955164 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 497 0.2049425377411786 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 488 0.20123130466337055 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 476 0.19628299389295978 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 464 0.19133468312254903 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 416 0.17154144004090602 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 377 0.1554594300370711 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 373 0.15380999311360083 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 371 0.1529852746518657 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 358 0.1476246046505874 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 346 0.14267629388017666 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 343 0.14143921618757396 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 309 0.12741900233807685 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 306 0.12618192464547415 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 303 0.12494484695287145 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 287 0.11834709925899047 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 286 0.1179347400281229 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 286 0.1179347400281229 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 286 0.1179347400281229 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 282 0.11628530310465264 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 271 0.11174935156510946 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.123592308675626E-4 0.0 0.0 0.0 6 0.0 4.123592308675626E-4 0.0 0.0 0.0 7 0.0 4.123592308675626E-4 0.0 4.123592308675626E-4 0.0 8 0.0 4.123592308675626E-4 0.0 4.123592308675626E-4 0.0 9 0.0 4.123592308675626E-4 0.0 4.123592308675626E-4 0.0 10-11 0.0 4.123592308675626E-4 0.0 4.123592308675626E-4 0.0 12-13 0.0 4.123592308675626E-4 0.0 4.123592308675626E-4 0.0 14-15 0.0 4.123592308675626E-4 0.0 4.123592308675626E-4 0.0 16-17 0.0 4.123592308675626E-4 0.0 8.247184617351252E-4 0.0 18-19 0.0 4.123592308675626E-4 0.0 8.247184617351252E-4 0.0 20-21 0.0 4.123592308675626E-4 0.0 8.247184617351252E-4 0.0 22-23 0.0 4.123592308675626E-4 0.0 0.0018556165389040317 0.0 24-25 0.0 4.123592308675626E-4 0.0 0.004948310770410752 0.0 26-27 0.0 4.123592308675626E-4 0.0 0.008247184617351251 0.0 28-29 0.0 4.123592308675626E-4 0.0 0.013401675003195784 0.0 30-31 0.0 4.123592308675626E-4 0.0 0.026390990775524006 0.0 32-33 0.0 4.123592308675626E-4 0.0 0.035669073470044166 0.0 34-35 0.0 4.123592308675626E-4 0.0 0.04247300077935895 0.0 36-37 0.0 4.123592308675626E-4 0.0 0.05278198155104801 0.0 38-39 0.0 4.123592308675626E-4 0.0 0.06824545270858161 0.0 40-41 0.0 4.123592308675626E-4 0.0 0.0991723950236488 0.0 42-43 0.0 4.123592308675626E-4 0.0 0.15834594465314406 0.0 44-45 0.0 4.123592308675626E-4 0.0 0.21566387774373524 0.0 46-47 0.0 4.123592308675626E-4 0.0 0.3371036712342324 0.0 48-49 0.0 4.123592308675626E-4 0.0 0.4535951539543188 0.0 50-51 0.0 4.123592308675626E-4 0.0 0.5401905924365069 0.0 52-53 0.0 4.123592308675626E-4 0.0 0.6257551328415262 0.0 54-55 0.0 4.123592308675626E-4 0.0 0.7065775420915685 0.0 56-57 0.0 4.123592308675626E-4 0.0 0.857913379819964 0.0 58-59 0.0 4.123592308675626E-4 0.0 1.030485717938039 0.0 60-61 0.0 4.123592308675626E-4 0.0 1.2430569014502675 0.0 62-63 0.0 4.123592308675626E-4 0.0 1.4455252838062407 0.0 64-65 0.0 4.123592308675626E-4 0.0 1.652529617701757 0.0 66-67 0.0 4.123592308675626E-4 0.0 1.9149962681489607 0.0 68-69 0.0 4.123592308675626E-4 0.0 2.210039297834702 0.0 70-71 0.0 4.123592308675626E-4 0.0 2.4669390986651933 0.0 72-73 0.0 4.123592308675626E-4 0.0 2.7287872102660953 0.0 74-75 0.0 4.123592308675626E-4 0.0 3.0114594630258096 0.0 76-77 0.0 4.123592308675626E-4 0.0 3.3091828277121897 0.0 78-79 0.0 4.123592308675626E-4 0.0 3.6398949308679747 0.0 80-81 0.0 4.123592308675626E-4 0.0 4.027512607883484 0.0 82-83 0.0 4.123592308675626E-4 0.0 4.398429736048856 0.0 84-85 0.0 4.123592308675626E-4 0.0 4.941300663486002 0.0 86-87 0.0 4.123592308675626E-4 0.0 5.472213173227989 0.0 88-89 0.0 4.123592308675626E-4 0.0 6.071164956063123 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGAACC 25 0.005016986 56.99864 9 ATGGGCG 40 5.3803547E-4 47.498867 7 GAGGCGC 40 5.3803547E-4 47.498867 9 CCGGACT 25 0.0016150842 37.999092 64-65 CGATTAG 25 0.0016150842 37.999092 18-19 TGGGCGG 50 0.0016134914 37.999092 8 ATGGGTA 115 2.4756446E-9 37.188362 5 TACATGA 170 0.0 36.337643 2 CATGGGT 300 0.0 34.84687 4 GGAGGCG 55 0.0025759323 34.544632 8 ATGGGGC 105 1.8877636E-6 31.678976 5 ATGAGAG 135 1.1845259E-8 31.678974 5 TGTAGCC 75 3.064346E-4 31.678974 2 AGCCGGA 30 0.0039491816 31.665913 62-63 GAGTCTA 60 0.003945322 31.665913 9 TCCGATT 30 0.0039491816 31.665913 16-17 CCCTATG 40 4.49048E-4 29.686792 62-63 GGGAGTC 80 4.4839317E-4 29.686792 7 CGCTAAT 40 4.49048E-4 29.686792 82-83 GAGTACC 65 0.0058181593 29.248163 1 >>END_MODULE