##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937837_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 221288 Sequences flagged as poor quality 0 Sequence length 101 %GC 31 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06112848414735 31.0 31.0 34.0 31.0 34.0 2 32.1698374968367 33.0 31.0 34.0 30.0 34.0 3 32.17447850764614 33.0 31.0 34.0 30.0 34.0 4 35.84459166335274 37.0 35.0 37.0 35.0 37.0 5 35.83245363508188 37.0 35.0 37.0 35.0 37.0 6 35.979135786847905 37.0 35.0 37.0 35.0 37.0 7 35.92341654314739 37.0 35.0 37.0 35.0 37.0 8 35.95031813744984 37.0 35.0 37.0 35.0 37.0 9 37.489755431835434 39.0 37.0 39.0 35.0 39.0 10-11 37.43111013701601 39.0 37.0 39.0 34.0 39.0 12-13 37.310536495426774 39.0 37.0 39.0 34.0 39.0 14-15 38.188699793933694 40.0 37.0 41.0 33.0 41.0 16-17 38.23782130074835 40.0 37.5 41.0 33.5 41.0 18-19 38.38877164599978 40.0 37.5 41.0 34.0 41.0 20-21 38.421690285962185 40.0 38.0 41.0 34.0 41.0 22-23 38.29612541122881 40.0 37.0 41.0 34.0 41.0 24-25 38.167085427135675 40.0 37.0 41.0 34.0 41.0 26-27 37.82537688442211 40.0 36.0 41.0 33.0 41.0 28-29 37.60062678500416 39.0 35.5 41.0 33.0 41.0 30-31 37.31240510104479 39.0 35.0 41.0 33.0 41.0 32-33 36.89091816998662 38.0 35.0 41.0 33.0 41.0 34-35 36.54842106214526 38.0 35.0 41.0 33.0 41.0 36-37 36.189334713134016 38.0 35.0 40.0 31.5 41.0 38-39 35.83888642854561 37.5 35.0 40.0 31.0 41.0 40-41 35.462232475326275 37.0 35.0 40.0 30.5 41.0 42-43 35.028078793246806 37.0 35.0 40.0 29.5 41.0 44-45 34.43370856078956 36.5 34.5 40.0 25.0 41.0 46-47 33.67874670113156 36.0 33.5 40.0 21.5 41.0 48-49 33.03540860778713 36.0 33.0 40.0 12.0 41.0 50-51 32.63392953978526 35.0 33.0 40.0 4.0 41.0 52-53 32.254512219370234 35.0 33.0 40.0 2.0 41.0 54-55 32.0308218249521 35.0 33.0 40.0 2.0 41.0 56-57 31.754103250063267 35.0 33.0 40.0 2.0 41.0 58-59 31.49221150717617 35.0 32.5 39.0 2.0 41.0 60-61 31.098432811539713 35.0 31.5 39.0 2.0 40.5 62-63 30.623377679765735 35.0 31.0 38.5 2.0 40.0 64-65 30.155342811178194 35.0 30.0 38.0 2.0 40.0 66-67 29.72411066121977 35.0 29.5 37.5 2.0 40.0 68-69 29.2532762734536 35.0 29.0 37.0 2.0 39.5 70-71 28.830153465167562 35.0 28.5 36.5 2.0 39.0 72-73 28.347447670004698 34.0 27.0 36.0 2.0 39.0 74-75 27.891546310690142 34.0 26.5 35.0 2.0 38.5 76-77 26.728781045515348 32.5 25.0 35.0 2.0 36.5 78-79 27.15177280286324 33.0 26.0 35.0 2.0 37.0 80-81 26.992498463540727 33.0 25.5 35.0 2.0 37.0 82-83 26.634808484870398 33.0 24.5 35.0 2.0 36.0 84-85 26.242301887133507 33.0 24.0 35.0 2.0 36.0 86-87 25.963723744622392 33.0 22.5 35.0 2.0 35.0 88-89 25.549209627273058 33.0 19.5 35.0 2.0 35.0 90-91 25.022868388706122 33.0 16.5 35.0 2.0 35.0 92-93 24.59756968294711 33.0 6.0 35.0 2.0 35.0 94-95 24.145100502512562 32.0 2.0 35.0 2.0 35.0 96-97 23.631683597845342 32.0 2.0 35.0 2.0 35.0 98-99 22.915130960558187 31.0 2.0 34.0 2.0 35.0 100-101 21.006057716640758 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3159949389810919 1101 2 0.2501026842709635 1101 3 0.1556322040915603 1101 4 0.23119474746361135 1101 5 0.20795002800925033 1101 6 0.17578911489954407 1101 7 0.2801887068033011 1101 8 0.22309867236008074 1101 9 0.3410140587594128 1101 10-11 0.30291227326834047 1101 12-13 0.33194294466301955 1101 14-15 0.2413608196670083 1101 16-17 0.29680594514564973 1101 18-19 0.26915780262814337 1101 20-21 0.3548778084426303 1101 22-23 0.2668489765416524 1101 24-25 0.49383563995274216 1101 26-27 0.5211400181750605 1101 28-29 0.628036946692113 1101 30-31 0.4431426894731416 1101 32-33 0.4171596482033735 1101 34-35 0.4678595994719146 1101 36-37 0.2333314531467181 1101 38-39 0.4629178833486307 1101 40-41 0.4700727270249203 1101 42-43 0.3605410569221661 1101 44-45 0.5567204612050034 1101 46-47 0.1643865978325323 1101 48-49 -0.004951014758404426 1101 50-51 0.08733546427630756 1101 52-53 0.126207616811989 1101 54-55 0.18906594845184088 1101 56-57 -0.05298405804886386 1101 58-59 0.09734205517173677 1101 60-61 0.10098840579203383 1101 62-63 0.47796845277548883 1101 64-65 0.47031472242621675 1101 66-67 0.08036451970554026 1101 68-69 0.4499171084623015 1101 70-71 0.042627296550517 1101 72-73 0.37532374397483537 1101 74-75 0.29303991852737354 1101 76-77 0.18047625681137092 1101 78-79 0.03144780652899115 1101 80-81 0.24432594584316192 1101 82-83 0.5177244391435671 1101 84-85 0.4450324999657411 1101 86-87 0.4249335534096197 1101 88-89 0.6569482087131995 1101 90-91 0.30876322119751975 1101 92-93 0.3228570295195077 1101 94-95 0.5161523445587797 1101 96-97 0.5458990342770775 1101 98-99 0.24643280775106646 1101 100-101 -0.1322209066334743 1102 1 0.07145208045536577 1102 2 0.11865938530188913 1102 3 0.05049450504504449 1102 4 0.21519842589156468 1102 5 0.036261823178897146 1102 6 -0.027807032431176992 1102 7 0.16220071009452397 1102 8 0.03384113400279176 1102 9 -0.13891188867778936 1102 10-11 -0.04416071448635961 1102 12-13 0.025260063873126626 1102 14-15 -0.1147993371501741 1102 16-17 0.22839807524583478 1102 18-19 0.24751678815643174 1102 20-21 0.18173759257698663 1102 22-23 0.1952587824561718 1102 24-25 0.208703022944853 1102 26-27 0.3563972661301449 1102 28-29 0.28728625698686727 1102 30-31 0.39682958749356345 1102 32-33 0.14865255754947526 1102 34-35 -0.07880440517451603 1102 36-37 0.13930768856610598 1102 38-39 0.07590249203165911 1102 40-41 0.1757682403108234 1102 42-43 -0.019752733310639314 1102 44-45 0.3045847949739411 1102 46-47 0.2905240064768364 1102 48-49 0.08242374928979501 1102 50-51 -0.2845306119058648 1102 52-53 0.2895467581894664 1102 54-55 0.021407074244393698 1102 56-57 -0.08000961344308877 1102 58-59 -0.06397032080232279 1102 60-61 0.284595201203679 1102 62-63 0.29347230060472995 1102 64-65 0.6992968626904847 1102 66-67 0.6505692532615157 1102 68-69 0.3443680062891268 1102 70-71 0.26485153545472784 1102 72-73 0.5343855028442057 1102 74-75 0.6245098376987634 1102 76-77 0.46802889221140376 1102 78-79 0.4311138439908788 1102 80-81 0.6529823516318451 1102 82-83 0.6841914542928826 1102 84-85 0.2735318465607719 1102 86-87 0.359841108707041 1102 88-89 0.4957749837966894 1102 90-91 0.43355751963418854 1102 92-93 0.8009921633465247 1102 94-95 0.6723282314955199 1102 96-97 0.23545907493338802 1102 98-99 0.02187930114441272 1102 100-101 0.3173640602122738 1103 1 0.41965829889809925 1103 2 0.4158291966226457 1103 3 0.40450726082500665 1103 4 0.24514793426254755 1103 5 0.27924614419935523 1103 6 0.377215555929169 1103 7 0.4369416083140294 1103 8 0.4028950803222173 1103 9 0.41714710680454203 1103 10-11 0.6154260513919283 1103 12-13 0.4936430030174179 1103 14-15 0.6115028153876878 1103 16-17 0.5280061007573948 1103 18-19 0.43018354340490106 1103 20-21 0.3805018763930903 1103 22-23 0.7371336163652913 1103 24-25 0.4591082847040937 1103 26-27 0.6106491927844075 1103 28-29 0.613156573223911 1103 30-31 0.7525896193830164 1103 32-33 0.6812263019023916 1103 34-35 0.5963949038873935 1103 36-37 0.8315678533282593 1103 38-39 0.7285665898259737 1103 40-41 0.5247119022826539 1103 42-43 0.5979591986585362 1103 44-45 0.6808337984286297 1103 46-47 0.05651523687811277 1103 48-49 0.15525128051488224 1103 50-51 0.6658480753117786 1103 52-53 0.5677689213571497 1103 54-55 0.3152152060541198 1103 56-57 0.10628456673230602 1103 58-59 -0.15043723549804255 1103 60-61 -0.07057354713541386 1103 62-63 -0.12193428988158317 1103 64-65 0.15241937132720906 1103 66-67 0.12181559926204599 1103 68-69 0.19931087102471068 1103 70-71 -0.03289627214558877 1103 72-73 -0.1397641118249453 1103 74-75 0.09631424907266606 1103 76-77 0.046669604409114385 1103 78-79 0.12532059392443884 1103 80-81 -0.18612208631659755 1103 82-83 0.08952629351690433 1103 84-85 0.16370590980817923 1103 86-87 0.06960060910565957 1103 88-89 0.5911244390185892 1103 90-91 0.45619238543275387 1103 92-93 0.9846089584568105 1103 94-95 0.8205743117506366 1103 96-97 1.1815924791321635 1103 98-99 1.2806090380564505 1103 100-101 2.009145883225827 1104 1 0.20967027853271958 1104 2 0.30913557577807893 1104 3 0.15137758128023648 1104 4 0.1619343972754237 1104 5 0.2569191001742013 1104 6 0.2335947113649297 1104 7 -0.10350463307560176 1104 8 0.11980133968130247 1104 9 0.3400731325607538 1104 10-11 0.16193698280851976 1104 12-13 -0.03303414582953934 1104 14-15 0.29644591212323945 1104 16-17 0.22895698425544708 1104 18-19 -0.061168657852959996 1104 20-21 0.19093164578512756 1104 22-23 0.12176224769203259 1104 24-25 0.17913673633631078 1104 26-27 0.03236976780686973 1104 28-29 0.19484270679684101 1104 30-31 0.13165520788256657 1104 32-33 0.610479072192895 1104 34-35 0.7003706451272862 1104 36-37 0.8595178383537174 1104 38-39 0.698415346938873 1104 40-41 0.5969341245048696 1104 42-43 0.5356351495420242 1104 44-45 0.6413833641668774 1104 46-47 0.8285577645810207 1104 48-49 0.775526812111174 1104 50-51 0.7816162693818569 1104 52-53 0.9616068967989051 1104 54-55 1.3106704321697293 1104 56-57 1.3807829195325496 1104 58-59 1.2471743248493716 1104 60-61 0.8601317538529081 1104 62-63 0.3084790075128474 1104 64-65 0.6226424920220275 1104 66-67 0.9284867582916974 1104 68-69 0.3353695712343523 1104 70-71 0.3500851594207468 1104 72-73 0.7414054000049468 1104 74-75 0.43213335658988683 1104 76-77 0.13111854121654076 1104 78-79 0.29261619140871886 1104 80-81 0.29352002209909145 1104 82-83 0.03177696737135349 1104 84-85 0.02210662858625767 1104 86-87 0.603525436898412 1104 88-89 0.5987539688179311 1104 90-91 0.6960770814495234 1104 92-93 0.5115331872678333 1104 94-95 0.7830481062999013 1104 96-97 0.86112976574492 1104 98-99 0.5711750757923006 1104 100-101 0.5778715495930022 1105 1 0.2284327997224267 1105 2 0.24730016200091853 1105 3 0.09250872629487361 1105 4 0.2387181852242577 1105 5 0.1493139221806743 1105 6 0.4103168565336759 1105 7 0.2952856273651392 1105 8 0.40362781525535496 1105 9 0.4440567972240288 1105 10-11 0.5571827833941327 1105 12-13 0.46764007207769254 1105 14-15 0.5161831597731066 1105 16-17 0.5729044337854248 1105 18-19 0.06949477256775793 1105 20-21 -0.08104785799049097 1105 22-23 0.29376486751018405 1105 24-25 0.18516617076381436 1105 26-27 -0.08870050496243209 1105 28-29 -0.11760913478121182 1105 30-31 -0.19589911481738653 1105 32-33 -0.0018841578887816013 1105 34-35 0.3437478989414373 1105 36-37 0.29350227355174496 1105 38-39 0.6560166722586516 1105 40-41 0.4790808343214863 1105 42-43 0.40056040767117196 1105 44-45 0.4259611111232644 1105 46-47 0.7957715109209715 1105 48-49 0.18279295481421087 1105 50-51 0.26115857173969914 1105 52-53 0.2375161278052147 1105 54-55 0.4035006063105797 1105 56-57 0.2744105971408075 1105 58-59 0.27065637138966636 1105 60-61 0.09978732481915742 1105 62-63 0.352630648751866 1105 64-65 0.7824706627725284 1105 66-67 0.4851788526051486 1105 68-69 0.3178399056464336 1105 70-71 0.4507362612854422 1105 72-73 0.04609410396549407 1105 74-75 0.6145959856544572 1105 76-77 0.7037221781405698 1105 78-79 1.0709333435122765 1105 80-81 0.879530963692563 1105 82-83 0.7767492281203126 1105 84-85 0.8413391759741451 1105 86-87 0.485729936821361 1105 88-89 0.348195331123371 1105 90-91 0.4041407276199642 1105 92-93 0.5019015028788658 1105 94-95 0.7072660135275193 1105 96-97 0.8478958313944815 1105 98-99 1.1706896388990948 1105 100-101 0.6035496201739861 1106 1 -0.1562207748583333 1106 2 -0.29043152378902093 1106 3 -0.3263460839434309 1106 4 0.09772083306185664 1106 5 -0.15595968770458768 1106 6 -0.10055031028009154 1106 7 -0.33059698516795777 1106 8 0.08985905973902675 1106 9 -0.39955529456767636 1106 10-11 -0.32485053522899676 1106 12-13 -0.41340030744569845 1106 14-15 -0.23119658426925582 1106 16-17 0.09331484861331774 1106 18-19 -0.24488077906507755 1106 20-21 -0.17617694007345364 1106 22-23 -0.21743327900349385 1106 24-25 -0.2573261063765315 1106 26-27 -0.377397548210439 1106 28-29 -0.6494935125393795 1106 30-31 -0.905547173069813 1106 32-33 -0.8859585179946947 1106 34-35 -1.1381033808467365 1106 36-37 -0.9584836046477179 1106 38-39 -0.4599089676354353 1106 40-41 -0.37812900680825834 1106 42-43 -0.42184140168452444 1106 44-45 -0.6431584828749735 1106 46-47 -0.25473047495722057 1106 48-49 -0.2763310522466895 1106 50-51 -0.6449259384103456 1106 52-53 -0.3509381969961929 1106 54-55 -0.3318259603266682 1106 56-57 -0.3209667197170951 1106 58-59 0.2556519583270038 1106 60-61 -0.09576651454536389 1106 62-63 0.27046493824877516 1106 64-65 0.11402957715911555 1106 66-67 0.09691208296701959 1106 68-69 0.47488977325455295 1106 70-71 0.6618185649041557 1106 72-73 0.5859219945215379 1106 74-75 0.6934518991584291 1106 76-77 0.4621069972050016 1106 78-79 1.0274646762572033 1106 80-81 0.6840322876113589 1106 82-83 0.6145691751635596 1106 84-85 0.7748122562918844 1106 86-87 0.7362815696545475 1106 88-89 0.7714632040271638 1106 90-91 0.06792051579295943 1106 92-93 0.037867963602611354 1106 94-95 -0.40413623713444125 1106 96-97 -0.5283453424801863 1106 98-99 -0.4515521969349727 1106 100-101 0.19768576315721376 1107 1 -0.12384762475262434 1107 2 0.03726403646467702 1107 3 -0.15602367379543836 1107 4 -0.35735316270981343 1107 5 -0.21594911688006135 1107 6 0.08879958401166732 1107 7 -0.014265061277896507 1107 8 0.09091614219145328 1107 9 0.1544880458128759 1107 10-11 -0.28818298766028505 1107 12-13 -0.561457918439352 1107 14-15 -0.6251580007597397 1107 16-17 -0.018735891344405786 1107 18-19 -0.0824864873103266 1107 20-21 -0.09233708806500118 1107 22-23 -0.42871763418320086 1107 24-25 -0.2975613072221961 1107 26-27 -0.33570885899268177 1107 28-29 -0.20129055270851381 1107 30-31 0.00942375716274313 1107 32-33 -0.06453938480230903 1107 34-35 -0.03675560071989281 1107 36-37 -0.3681030549648412 1107 38-39 -0.4121420543161918 1107 40-41 -0.6688927488801468 1107 42-43 -0.8897985898451992 1107 44-45 -0.8636923095134463 1107 46-47 -0.5914773037098655 1107 48-49 -0.14247798670758272 1107 50-51 -0.2898123020467125 1107 52-53 -0.12029601940633938 1107 54-55 0.22419940965219354 1107 56-57 0.29597302658311975 1107 58-59 -0.1981006843791313 1107 60-61 -0.10567666253690433 1107 62-63 -0.24730726048272444 1107 64-65 -0.023721479923338507 1107 66-67 0.027739249986051817 1107 68-69 0.12380626374081416 1107 70-71 -0.14691557885905837 1107 72-73 0.19480148712196055 1107 74-75 0.3269746264311557 1107 76-77 0.09503511559823252 1107 78-79 0.40695727668003556 1107 80-81 0.7500999408883153 1107 82-83 0.5654148411255058 1107 84-85 0.8374443915984386 1107 86-87 1.0803619079209312 1107 88-89 0.9613416395451324 1107 90-91 0.7622915517337638 1107 92-93 0.8050455534546153 1107 94-95 0.7313685197365949 1107 96-97 0.7766890126995172 1107 98-99 1.074082052536486 1107 100-101 1.6939199069204314 1108 1 -0.035843869155335994 1108 2 0.08605068964971707 1108 3 -0.13778736005861703 1108 4 0.2714927133554781 1108 5 0.2481199283315476 1108 6 0.1502613780316011 1108 7 -0.2806957993972077 1108 8 0.05061569440870528 1108 9 -0.1877046452170248 1108 10-11 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-0.46187680329773073 2316 82-83 -0.6192944612000773 2316 84-85 -0.8333695618899313 2316 86-87 -0.7216729758000007 2316 88-89 -0.5583095232455619 2316 90-91 -0.907534029661587 2316 92-93 -1.628268400033754 2316 94-95 -1.5883407825889897 2316 96-97 -1.0948226152074625 2316 98-99 -1.2713249242077005 2316 100-101 -1.2428824186609653 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 10.0 5 19.0 6 38.0 7 86.0 8 153.0 9 280.0 10 412.0 11 728.0 12 1124.0 13 1663.0 14 2012.0 15 2887.0 16 4135.0 17 5736.0 18 4983.0 19 2720.0 20 2281.0 21 2247.0 22 2377.0 23 2587.0 24 2713.0 25 3002.0 26 3302.0 27 3587.0 28 4204.0 29 4868.0 30 5845.0 31 7049.0 32 8885.0 33 11769.0 34 17557.0 35 30618.0 36 27211.0 37 29651.0 38 22421.0 39 2125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.28103115435216 22.752939793570203 13.83658319355845 12.129445858519185 2 15.219532916380462 29.10460576262608 37.6093597483822 18.06650157261126 3 22.20409601966668 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17.71218629327272 36.57853768095967 31.599675532287474 14.109600493480142 24-25 16.516033404432235 37.833727992480384 32.64252919272622 13.00770941036116 26-27 16.750494834738756 37.13565250399935 33.54776172012689 12.566090941135002 28-29 14.486941078380644 39.36249776871959 33.456100829019526 12.694460323880236 30-31 14.59207006254293 41.04289432775388 31.989759950833303 12.375275658869889 32-33 14.261053468782764 41.310870901268935 31.3873775351578 13.040698094790498 34-35 14.677253172336503 40.523435522938435 31.546220310184015 13.25309099454105 36-37 15.233350279613624 40.915776986951364 31.756425464610516 12.094447268824492 38-39 15.356684501644915 41.20512635118036 31.494929684393192 11.943259462781533 40-41 13.549207812514123 42.55917681190857 31.474878663810635 12.416736711766672 42-43 13.31159145150792 43.702312504520144 29.595176104722643 13.390919939249295 44-45 14.280937059397708 43.29335526553631 28.565715266982394 13.859992408083583 46-47 14.546880083872601 43.47253353096418 27.5941307255703 14.386455659592928 48-49 15.231960160514804 43.72830881023824 27.315986406854414 13.72374462239254 50-51 15.245969053902606 44.609061494522976 26.986099562561005 13.158869889013413 52-53 14.335390983695456 45.31967390911392 26.628420881385345 13.716514225805284 54-55 13.453960449730667 44.96583637612523 26.29514478869166 15.28505838545244 56-57 14.872699830085681 45.511505368569466 24.91775423882 14.698040562524856 58-59 15.36820794620585 44.41045153826688 25.219171396551097 15.002169118976175 60-61 14.543038935685622 44.80134485376523 26.186462889989514 14.469153320559633 62-63 15.88043635443404 44.04124941253028 26.068968945446656 14.009345287589026 64-65 15.090063627489968 44.9346552908427 26.16228625140089 13.81299483026644 66-67 14.65895123097502 44.15670980803297 25.938821806876106 15.245517154115904 68-69 14.276644011424027 45.201502114891 25.573008929539785 14.948844944145186 70-71 15.01662991215068 45.051471385705504 24.58402624633961 15.3478724558042 72-73 14.689680416470843 43.89280033259824 25.31226275261198 16.10525649831893 74-75 14.633192943132931 45.14094754347276 24.985087307038793 15.240772206355519 76-77 14.981381728787824 43.2906438668161 26.173809695961825 15.554164708434257 78-79 13.974774953906222 43.4971620693395 26.60356639311666 15.924496583637612 80-81 13.773679548823253 44.889691262065725 25.74879794656737 15.587831242543654 82-83 13.764898604756256 43.974919505168614 25.32610292040897 16.934078969666157 84-85 13.703214144495282 43.33005705247698 25.32994407727504 17.636784725752698 86-87 14.007085788655507 43.675210585300604 25.338924840027477 16.978778786016413 88-89 14.125483532771774 42.8326434329923 25.63401540074473 17.407857633491197 90-91 13.382732453821086 42.94514573418483 26.025156038850394 17.64696577314369 92-93 12.991931165224702 44.35486604288164 25.037790888159307 17.615411903734348 94-95 12.746059433859946 44.11671667690973 24.453427207982358 18.683796681247966 96-97 12.696350457322584 43.500777267633126 24.433543617367413 19.369328657676874 98-99 13.978127753801658 43.92050974987008 23.584065797500962 18.517296698827305 100-101 13.714381275437912 43.95603153905432 23.61835845956714 18.711228725940625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6503.0 1 3805.5 2 1288.5 3 1652.0 4 1828.0 5 2032.5 6 3079.0 7 4717.0 8 5336.5 9 4701.5 10 4001.5 11 3801.0 12 3881.0 13 3789.5 14 3377.0 15 2723.5 16 2151.5 17 1851.0 18 1740.0 19 1723.0 20 1699.5 21 1651.5 22 1681.5 23 1828.0 24 1972.5 25 2151.5 26 2483.5 27 2814.5 28 3002.5 29 3238.0 30 3481.5 31 3767.5 32 4265.0 33 4848.0 34 5472.0 35 5800.0 36 6083.0 37 6546.5 38 6331.5 39 5770.5 40 5478.5 41 5162.0 42 4740.5 43 4533.5 44 4426.5 45 4068.0 46 3941.5 47 5074.0 48 11106.5 49 10876.5 50 5445.0 51 3988.0 52 2235.0 53 1594.5 54 1351.5 55 1261.0 56 1287.5 57 1258.0 58 1155.5 59 1196.5 60 1283.5 61 1372.0 62 1233.5 63 648.0 64 231.0 65 138.0 66 107.0 67 90.5 68 75.5 69 62.5 70 44.5 71 30.0 72 35.5 73 36.0 74 31.0 75 22.0 76 15.0 77 9.5 78 5.0 79 4.0 80 3.0 81 3.5 82 2.5 83 2.0 84 1.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04473807888362677 2 0.0 3 0.0 4 0.0 5 0.010845594880879215 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0049708976537363074 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.2594989335165033E-4 22-23 0.0011297494667582518 24-25 0.0 26-27 9.037995734066013E-4 28-29 0.0011297494667582518 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.2594989335165033E-4 38-39 0.0 40-41 9.037995734066013E-4 42-43 0.025306388055384835 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.2594989335165033E-4 84-85 2.2594989335165033E-4 86-87 0.0 88-89 0.0 90-91 0.01310509381439572 92-93 0.002033549040164853 94-95 0.0 96-97 0.0 98-99 0.0013556993601099019 100-101 0.017398141788077075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 221288.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.24051404464332 #Duplication Level Percentage of deduplicated Percentage of total 1 79.94173360068399 36.96546855315563 2 10.998987871748238 10.17197706320872 3 3.513359330325785 4.873786243734243 4 1.604410853902322 2.96755130492994 5 0.9036584682612673 2.0892816046598 6 0.5607877041320596 1.555866702538907 7 0.38885277897434806 1.258652667122335 8 0.27189705824797833 1.0058127792490277 9 0.21591825211171234 0.8985753872339824 >10 1.3944720448777084 11.988863075366693 >50 0.11695571989296287 3.8055407217292343 >100 0.07097312916581508 6.486475519012067 >500 0.010995836913013604 3.7986072696672957 >1k 0.005997729225280147 8.224923066008746 >5k 9.996215375466912E-4 3.908618042383384 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8456 3.82126459636311 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4509 2.0376161382451827 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3859 1.7438812768880374 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2834 1.280683995517154 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2578 1.164997650121109 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2097 0.9476338527168214 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1917 0.8662918911102274 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 984 0.44466939011604784 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 943 0.4261414988612125 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 907 0.40987310653989373 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 822 0.3714616246701132 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 758 0.3425400383211019 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 758 0.3425400383211019 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 668 0.30186905751780485 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 638 0.28831206391670583 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 634 0.28650446476989266 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 591 0.2670727739416507 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 515 0.23272839015219984 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 469 0.21194099996384802 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 443 0.2001916055095622 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 424 0.1916055095621995 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 408 0.1843751129749467 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 379 0.17127001916055096 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 373 0.16855862044033115 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 341 0.15409782726582552 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 313 0.1414446332381331 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 310 0.1400889338780232 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 294 0.1328585372907704 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 284 0.1283395394237374 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 276 0.12472434113011098 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 271 0.12246484219659448 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 264 0.11930154368967139 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 261 0.11794584432956148 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 260 0.11749394454285816 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 259 0.11704204475615487 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 250 0.11297494667582517 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 242 0.10935974838219877 No Hit GTACATGGGAATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 234 0.10574455008857235 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 231 0.10438885072846246 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 231 0.10438885072846246 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.2594989335165033E-4 0.0 16-17 0.0 0.0 0.0 6.77849680054951E-4 0.0 18-19 0.0 0.0 0.0 9.037995734066013E-4 0.0 20-21 0.0 0.0 0.0 9.037995734066013E-4 0.0 22-23 0.0 0.0 0.0 0.002033549040164853 0.0 24-25 0.0 0.0 0.0 0.003841148186978056 0.0 26-27 0.0 0.0 0.0 0.008586095947362713 0.0 28-29 0.0 0.0 0.0 0.016042442427967173 0.0 30-31 0.0 0.0 0.0 0.02575828784208814 0.0 32-33 0.0 0.0 0.0 0.036829832616319 0.0 34-35 0.0 0.0 0.0 0.04609377824373667 0.0 36-37 0.0 0.0 0.0 0.055583673764505986 0.0 38-39 0.0 0.0 0.0 0.07072231661906656 0.0 40-41 0.0 0.0 0.0 0.11003759806225372 0.0 42-43 0.0 0.0 0.0 0.1674288709735729 0.0 44-45 0.0 0.0 0.0 0.21917139655110085 0.0 46-47 0.0 0.0 0.0 0.33915078992082714 0.0 48-49 0.0 0.0 0.0 0.4620675319041249 0.0 50-51 0.0 0.0 0.0 0.5427316438306642 0.0 52-53 0.0 0.0 0.0 0.6304002024511044 0.0 54-55 0.0 0.0 0.0 0.711516214164347 0.0 56-57 0.0 0.0 0.0 0.8651621416434692 0.0 58-59 0.0 0.0 0.0 1.0621904486461082 0.0 60-61 0.0 0.0 0.0 1.288592241784462 0.0 62-63 0.0 0.0 0.0 1.5138642854560573 0.0 64-65 0.0 0.0 0.0 1.7350692310473228 0.0 66-67 0.0 0.0 0.0 2.0014641553089185 0.0 68-69 0.0 0.0 0.0 2.301073713893207 0.0 70-71 0.0 0.0 0.0 2.5896117277032644 0.0 72-73 0.0 0.0 0.0 2.863914898232168 0.0 74-75 0.0 0.0 0.0 3.1395737681211813 0.0 76-77 0.0 0.0 0.0 3.450028921586349 0.0 78-79 0.0 0.0 0.0 3.7950544087343188 0.0 80-81 0.0 0.0 0.0 4.221421857488883 0.0 82-83 0.0 0.0 0.0 4.5908499331188315 0.0 84-85 0.0 0.0 0.0 5.140811973536749 0.0 86-87 0.0 0.0 0.0 5.638805538483785 0.0 88-89 0.0 0.0 0.0 6.217011315570659 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGCA 15 6.6075526E-4 95.01175 4 TATCACG 15 6.6075526E-4 95.01175 2 ATCACGC 15 6.6075526E-4 95.01175 3 CGTCACT 25 0.0050000274 57.04574 1 ATGGGCC 40 5.3754105E-4 47.50588 5 CCTTATA 40 5.3754105E-4 47.50588 2 TCCAGGG 65 1.323756E-4 36.54298 2 GTCCAGG 55 0.0025650305 34.573174 1 ACCTGGG 155 3.6379788E-11 33.71385 3 TACCTGG 155 3.6379788E-11 33.71385 2 ATGGGTC 60 0.00394173 31.670582 5 GCCTTAG 60 0.00394173 31.670582 7 GAGCTGG 60 0.0039460957 31.663425 6 GGGAGAC 110 2.7176884E-6 30.231012 7 ATGGGTT 65 0.005830484 29.234383 5 GTCTATG 65 0.005830484 29.234383 2 ATGGGAG 850 0.0 28.503527 5 CATGGGC 355 0.0 28.102068 4 TGGGAGG 355 0.0 28.095718 6 GGATAGC 85 6.360793E-4 27.963594 1 >>END_MODULE