FastQCFastQC Report
Thu 26 May 2016
SRR937836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937836_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183992
Sequences flagged as poor quality0
Sequence length101
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82484.482803600156529No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33121.8000782642723596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26891.461476585938519No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21661.1772250967433369No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20331.1049393451889213No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16900.9185181964433237No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14670.7973172746641157No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7210.3918648636897257No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.38642984477585984No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT6780.3684942823601026No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6700.36414626722900995No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6220.338058176442454No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG5780.31414409322144443No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT5450.2962085308056872No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG5420.2945780251315274No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG5180.28153397973824945No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.24674985868950822No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.20055219792164874No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3560.19348667333362318No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3130.17011609200400019No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT3070.16685508065568067No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA3010.1635940693073612No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC2760.15000652202269663No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2740.14891951823992347No Hit
GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA2740.14891951823992347No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA2740.14891951823992347No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC2610.1418539936518979No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG2550.13859298230357842No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT2350.12772294447584678No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG2310.12554893691030045No Hit
GTACATGGGAATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2270.12337492934475411No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2190.11902691421366146No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA2140.11630940475672856No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2020.10978738206008958No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA1940.10543936692899691No Hit
GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA1880.10217835558067742No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTAG200.00207139471.249186
AGTGACC250.00501481856.9993488
CGCTAAT205.383679E-447.49945482-83
CTCGCTA205.383679E-447.49945480-81
GGGGGAT608.264162E-539.5828827
CGTCATC250.001614478637.99956540-41
AGGAGCC801.0727834E-535.624597
GGATTGT550.00257416434.5450638
GGGAGAT702.0467959E-433.928187
ATGGGGG2800.032.2317735
ATCTCGC300.003947712531.66630678-79
GGGAGCA600.003942625631.6663067
GGAGTCT753.0686843E-431.6663068
GGGATAC600.003942625631.6663067
GGGGGAC902.4022296E-531.6663027
ATGGGAG7700.030.2269275
TCTATAT804.4794416E-429.687163
GGGAGTC650.00583181329.2304337
GGAACAT650.00583181329.2304338
AGTACTC650.00583181329.2304335
Produced by FastQC (version 0.11.5)