##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937836_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 183992 Sequences flagged as poor quality 0 Sequence length 101 %GC 30 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04140397408583 31.0 31.0 34.0 31.0 34.0 2 32.155653506674206 33.0 31.0 34.0 30.0 34.0 3 32.21402560980912 34.0 31.0 34.0 30.0 34.0 4 35.88429388234271 37.0 35.0 37.0 35.0 37.0 5 35.79751837036393 37.0 35.0 37.0 35.0 37.0 6 35.98825492412713 37.0 35.0 37.0 35.0 37.0 7 35.939606069829125 37.0 35.0 37.0 35.0 37.0 8 36.01515826775077 37.0 35.0 37.0 35.0 37.0 9 37.52572937953824 39.0 37.0 39.0 35.0 39.0 10-11 37.50068481238315 39.0 37.0 39.0 35.0 39.0 12-13 37.42037153789295 39.0 37.0 39.0 34.5 39.0 14-15 38.28540099569547 40.0 38.0 41.0 33.0 41.0 16-17 38.34667539893039 40.0 37.5 41.0 33.5 41.0 18-19 38.47804795860689 40.0 38.0 41.0 34.0 41.0 20-21 38.53415909387365 40.0 38.0 41.0 34.0 41.0 22-23 38.3454443671464 40.0 37.5 41.0 34.0 41.0 24-25 38.165251750076095 40.0 37.0 41.0 34.0 41.0 26-27 37.88037795121527 40.0 36.0 41.0 33.5 41.0 28-29 37.64269098656463 39.0 35.5 41.0 33.0 41.0 30-31 37.35591764859342 39.0 35.0 41.0 33.0 41.0 32-33 36.95568285577634 38.0 35.0 41.0 33.0 41.0 34-35 36.64147625983738 38.0 35.0 41.0 33.0 41.0 36-37 36.304363776685946 38.0 35.0 40.0 32.5 41.0 38-39 35.951429953476236 37.5 35.0 40.0 31.5 41.0 40-41 35.563538088612546 37.0 35.0 40.0 31.0 41.0 42-43 35.171477564241926 37.0 35.0 40.0 30.5 41.0 44-45 34.57377494673682 36.0 35.0 40.0 26.5 41.0 46-47 33.83761250489152 36.0 34.0 40.0 22.5 41.0 48-49 33.20936236358102 35.0 33.0 40.0 17.5 41.0 50-51 32.799178768642115 35.0 33.0 40.0 8.5 41.0 52-53 32.44649223879299 35.0 33.0 40.0 2.0 41.0 54-55 32.21775131527458 35.0 33.0 40.0 2.0 41.0 56-57 31.938350580460018 35.0 33.0 39.5 2.0 41.0 58-59 31.697603700160876 35.0 33.0 39.0 2.0 41.0 60-61 31.299654332797076 35.0 32.5 39.0 2.0 40.5 62-63 30.857045415018042 35.0 31.5 38.5 2.0 40.0 64-65 30.377576198965173 35.0 31.0 38.0 2.0 40.0 66-67 29.93461943997565 35.0 30.0 37.5 2.0 40.0 68-69 29.509867276838122 35.0 29.5 37.0 2.0 39.5 70-71 29.11628222966216 35.0 29.0 36.0 2.0 39.0 72-73 28.66921931388321 34.5 28.5 36.0 2.0 39.0 74-75 28.24422257489456 34.0 27.5 35.0 2.0 38.5 76-77 27.117304013217968 33.0 26.0 35.0 2.0 36.5 78-79 27.496298752119657 33.5 26.5 35.0 2.0 37.0 80-81 27.31423648854298 34.0 26.0 35.0 2.0 37.0 82-83 26.983800926127223 33.5 25.5 35.0 2.0 36.0 84-85 26.60715411539632 33.0 24.5 35.0 2.0 36.0 86-87 26.148174920648724 33.0 24.0 35.0 2.0 35.0 88-89 25.66470281316579 33.0 21.0 35.0 2.0 35.0 90-91 25.150112504891517 33.0 19.0 35.0 2.0 35.0 92-93 24.864524001043524 33.0 14.0 35.0 2.0 35.0 94-95 24.52672127049002 33.0 4.5 35.0 2.0 35.0 96-97 24.05217889908257 32.0 2.0 35.0 2.0 35.0 98-99 23.41553437105961 32.0 2.0 34.5 2.0 35.0 100-101 21.631008413409276 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1514517926878156 1101 2 0.38342356968241376 1101 3 0.1917577885949484 1101 4 0.31097938353693166 1101 5 -0.03185270942173446 1101 6 0.00650803813556422 1101 7 0.05565701198723616 1101 8 0.2931986664583661 1101 9 0.23667444381547398 1101 10-11 0.4195747498635356 1101 12-13 0.23826012304824928 1101 14-15 0.34424934059651235 1101 16-17 0.037481335102725666 1101 18-19 -0.053471680927287935 1101 20-21 0.3837103480799655 1101 22-23 0.4381724008005108 1101 24-25 0.43493929371248186 1101 26-27 0.521123221729745 1101 28-29 0.4874079150696389 1101 30-31 0.6806036031816305 1101 32-33 0.6008837759733439 1101 34-35 0.7954677797893552 1101 36-37 0.9950949856689704 1101 38-39 1.087987697452327 1101 40-41 1.273081996559256 1101 42-43 1.2501385644863134 1101 44-45 1.4341280809390682 1101 46-47 1.5175139331763887 1101 48-49 1.5998218963418012 1101 50-51 2.182769981231168 1101 52-53 1.7407082074403988 1101 54-55 1.5222935023745379 1101 56-57 1.6134918007686743 1101 58-59 1.591990420109699 1101 60-61 1.5902845313703295 1101 62-63 1.696941028828924 1101 64-65 1.641827575908664 1101 66-67 1.8421721242833868 1101 68-69 1.5243896360952789 1101 70-71 1.7623886383799459 1101 72-73 1.6966090400750886 1101 74-75 1.1283627965668224 1101 76-77 0.9399907660317695 1101 78-79 1.0255999545406773 1101 80-81 1.5426470361846825 1101 82-83 1.269731441913077 1101 84-85 1.1618714612303052 1101 86-87 1.501593450727146 1101 88-89 1.9942839944283186 1101 90-91 1.6412047905070466 1101 92-93 1.5902998018986096 1101 94-95 1.4638892470500657 1101 96-97 1.5933445246317426 1101 98-99 1.6800279553048227 1101 100-101 1.877040037559226 1102 1 0.3105370135612944 1102 2 0.25609282259193833 1102 3 0.16988943669906575 1102 4 0.3880973912956591 1102 5 0.36506257026791644 1102 6 0.2361726608361039 1102 7 0.2843205084207199 1102 8 0.232630527301815 1102 9 0.37680083600669434 1102 10-11 0.44907668954567015 1102 12-13 0.4177745768087249 1102 14-15 0.0847542836574533 1102 16-17 -0.02734159068879194 1102 18-19 0.3300378923426379 1102 20-21 0.2551438357536 1102 22-23 0.45641166997833693 1102 24-25 0.41705980428687894 1102 26-27 0.5742454842751172 1102 28-29 0.5723315791917756 1102 30-31 0.5283385550024278 1102 32-33 0.6292281634092092 1102 34-35 0.47448167041631706 1102 36-37 0.6557131811388643 1102 38-39 0.8260680378352632 1102 40-41 0.7843290244656487 1102 42-43 1.0782733411100125 1102 44-45 0.8780493673863248 1102 46-47 0.7865979026420362 1102 48-49 0.6109281408668963 1102 50-51 0.5480402853232746 1102 52-53 0.2528312208304584 1102 54-55 0.07413307189024465 1102 56-57 0.1959752196148692 1102 58-59 0.15725133864305008 1102 60-61 0.5343309586009646 1102 62-63 0.7602153374260965 1102 64-65 0.5059246851565717 1102 66-67 0.3237764291263332 1102 68-69 0.6830681494227733 1102 70-71 1.0048489061812198 1102 72-73 1.0164532397997803 1102 74-75 0.5826707680347241 1102 76-77 0.6534340146865034 1102 78-79 1.1349573566180133 1102 80-81 0.8952624785573562 1102 82-83 0.7147946380086871 1102 84-85 0.5791534034152654 1102 86-87 0.3185750550319888 1102 88-89 0.469897184266884 1102 90-91 0.36910179964232626 1102 92-93 0.8070592813129522 1102 94-95 0.23306707210324973 1102 96-97 0.7255870474699364 1102 98-99 1.1894760844501064 1102 100-101 1.1895009310644191 1103 1 -0.04207379396754618 1103 2 0.10020332653850517 1103 3 -0.09050825607567958 1103 4 0.14986120428898175 1103 5 0.04607349922966364 1103 6 -0.18542721773098947 1103 7 -0.23381853225929916 1103 8 -0.052433224975054316 1103 9 0.00385001633456028 1103 10-11 -0.08682191397588213 1103 12-13 -0.12802839829752344 1103 14-15 0.07249714947408847 1103 16-17 0.020358773609927994 1103 18-19 -0.011416266734471492 1103 20-21 0.05705640405961532 1103 22-23 -0.11694139620260557 1103 24-25 0.10091681703733713 1103 26-27 0.18296588500371058 1103 28-29 0.24594269030288984 1103 30-31 -0.08869241038919284 1103 32-33 -0.27871051297208993 1103 34-35 -0.04783162648714523 1103 36-37 -0.2817109232934314 1103 38-39 0.06900975003927101 1103 40-41 -0.08771104839409816 1103 42-43 0.18171899988354312 1103 44-45 0.26838027206149206 1103 46-47 0.3097460507901886 1103 48-49 0.3914002112979773 1103 50-51 0.8632725257604292 1103 52-53 0.060170322226937856 1103 54-55 0.13902378227275847 1103 56-57 0.2870499008534857 1103 58-59 0.2999350059168826 1103 60-61 0.13811814742913953 1103 62-63 0.2697781789561482 1103 64-65 0.011844539437078083 1103 66-67 -0.13994548951361452 1103 68-69 -0.06408576678852285 1103 70-71 0.39931611929597466 1103 72-73 0.1965913697329924 1103 74-75 0.01163251666860532 1103 76-77 0.45637117315644815 1103 78-79 0.4043107263812189 1103 80-81 0.3136747432810978 1103 82-83 0.35470811706150585 1103 84-85 0.4567718005008743 1103 86-87 0.9061205923726092 1103 88-89 0.7924533469869814 1103 90-91 1.038045333339955 1103 92-93 1.3256157476153199 1103 94-95 1.6668861370698593 1103 96-97 1.360908844676981 1103 98-99 1.2718607231872099 1103 100-101 1.0396071848591966 1104 1 0.07940768751393534 1104 2 0.12724036357554525 1104 3 -0.23180455237197606 1104 4 -0.09884249941472234 1104 5 0.05885127700744164 1104 6 -0.07339018069394854 1104 7 0.24044072699995667 1104 8 0.08312233058050111 1104 9 -0.02503887255432602 1104 10-11 -0.012562654716624877 1104 12-13 0.02150863873951181 1104 14-15 0.012960112437049531 1104 16-17 -0.20825233750117889 1104 18-19 -0.07326811858631999 1104 20-21 -0.13794359594039207 1104 22-23 0.02537341861221165 1104 24-25 0.042861261481782265 1104 26-27 0.22435477389259972 1104 28-29 0.16510935696955187 1104 30-31 0.5613075896108128 1104 32-33 0.17110430184272474 1104 34-35 0.056612817957294226 1104 36-37 0.07782611374360471 1104 38-39 -0.1337680277385047 1104 40-41 0.09840006271701185 1104 42-43 -0.007910629746085363 1104 44-45 9.728646540878572E-4 1104 46-47 0.32659790264203536 1104 48-49 -0.06628497388719978 1104 50-51 -0.032005252017341945 1104 52-53 -0.13918152962491348 1104 54-55 0.26402378227275136 1104 56-57 0.5542721230757088 1104 58-59 0.2984535244353985 1104 60-61 0.20098851779950877 1104 62-63 0.8403337345117059 1104 64-65 0.35740009499263437 1104 66-67 0.7026471030789736 1104 68-69 1.1145253443225869 1104 70-71 1.5912605637404198 1104 72-73 1.4931654438070652 1104 74-75 1.7588547388908253 1104 76-77 0.8844267287120076 1104 78-79 0.7220885041589966 1104 80-81 0.8394154840218349 1104 82-83 0.7266525615059543 1104 84-85 0.7764940227230959 1104 86-87 0.8669539257059427 1104 88-89 0.8815274210610582 1104 90-91 0.6341564444510688 1104 92-93 0.2842268587264307 1104 94-95 -0.006076825893106985 1104 96-97 0.345168103936242 1104 98-99 0.5741755380020237 1104 100-101 0.5059034811554959 1105 1 0.3246929727992196 1105 2 0.3358890122241931 1105 3 0.27558283501541325 1105 4 0.1923286717564423 1105 5 -0.010458032301862374 1105 6 0.2004836931799261 1105 7 0.13737766393689554 1105 8 0.17068989814806912 1105 9 0.1038500163345617 1105 10-11 0.09068058852661665 1105 12-13 0.0401272573581295 1105 14-15 0.19127843075536788 1105 16-17 0.35072664147779875 1105 18-19 0.3939390886208898 1105 20-21 0.258632980636186 1105 22-23 0.3214094546482471 1105 24-25 0.41379219241270704 1105 26-27 0.1230935126313355 1105 28-29 -0.30131706945687853 1105 30-31 0.02497125327447236 1105 32-33 8.940916325101966E-4 1105 34-35 -0.008792587448105849 1105 36-37 -0.17826998235248936 1105 38-39 -0.2121464061168865 1105 40-41 -0.2646630003460544 1105 42-43 -0.5482109300463875 1105 44-45 -0.931819928138701 1105 46-47 -0.391780475736347 1105 48-49 -0.7252038928061282 1105 50-51 -0.5192725192846126 1105 52-53 -0.3545569050002868 1105 54-55 -0.42876901052003547 1105 56-57 -0.4615236727200873 1105 58-59 -0.15271764673576982 1105 60-61 8.683976793868453E-4 1105 62-63 -0.3047713705934001 1105 64-65 -0.542059364466823 1105 66-67 -0.5282838278519577 1105 68-69 -0.3777869679897208 1105 70-71 -0.2861568536769994 1105 72-73 -0.2770447664031437 1105 74-75 -0.5543584743223882 1105 76-77 -0.32211981783454036 1105 78-79 0.038845260915756796 1105 80-81 -0.04851244390609111 1105 82-83 0.08845436330775414 1105 84-85 0.13511264134171697 1105 86-87 0.1446716434236599 1105 88-89 0.2691550486886847 1105 90-91 0.6813636516582768 1105 92-93 0.7893920238915939 1105 94-95 0.5866559068396242 1105 96-97 0.8295524883206262 1105 98-99 1.2642656280921116 1105 100-101 1.596113691365705 1106 1 -0.06386028634227259 1106 2 0.03009750643268916 1106 3 0.0820329826420334 1106 4 0.14608747257406662 1106 5 0.002305992226865783 1106 6 -0.04930054483961044 1106 7 0.1511410071120025 1106 8 0.1879962801603341 1106 9 0.3946063188555655 1106 10-11 -0.019789545472924885 1106 12-13 0.14401097300655152 1106 14-15 0.1414941927358413 1106 16-17 0.2527934141328103 1106 18-19 0.2718859430383276 1106 20-21 0.027994779409745263 1106 22-23 -0.24767980267630207 1106 24-25 -0.17353463020823057 1106 26-27 -0.04701777512701 1106 28-29 0.24985258759513584 1106 30-31 -0.4932208884470768 1106 32-33 -0.547345744842616 1106 34-35 -0.8891014677569942 1106 36-37 -1.0360487695430436 1106 38-39 -1.5984739100914496 1106 40-41 -1.5567820101121228 1106 42-43 -1.193475522369802 1106 44-45 -1.8547134098557194 1106 46-47 -1.5243264671058583 1106 48-49 -0.9046043016182992 1106 50-51 -0.7955159896457289 1106 52-53 -0.9016838638919538 1106 54-55 -1.1365364418168795 1106 56-57 -0.9530294642258781 1106 58-59 -0.5484932542760852 1106 60-61 -0.720094582107123 1106 62-63 -0.5204879274864282 1106 64-65 -0.7162133503855159 1106 66-67 -0.4434780136343761 1106 68-69 -0.24539995913212564 1106 70-71 -0.5266292588552872 1106 72-73 -0.33957031716398944 1106 74-75 -0.3829753264686495 1106 76-77 -0.019644233006012257 1106 78-79 -0.6624493109670553 1106 80-81 -0.84719516023587 1106 82-83 -0.9013026205668737 1106 84-85 -1.0870540631779342 1106 86-87 -0.9964821153771588 1106 88-89 -0.5782671634394134 1106 90-91 -1.0675802502268041 1106 92-93 -1.1764267360448102 1106 94-95 -1.2837052105803153 1106 96-97 -0.5410130351860758 1106 98-99 0.2167525688143499 1106 100-101 0.6872106706979793 1107 1 -0.42981551799245565 1107 2 0.028833283929522224 1107 3 -0.1607721039943968 1107 4 -0.12869500678935708 1107 5 -0.4181595391184132 1107 6 -0.1612957854137136 1107 7 -0.2957244647404522 1107 8 -0.3862582003929518 1107 9 -0.8147340544619013 1107 10-11 -0.3994653095838814 1107 12-13 -0.009071498920270926 1107 14-15 -0.5058992779267655 1107 16-17 0.12317342454404923 1107 18-19 0.3454339463104361 1107 20-21 0.05963752500355923 1107 22-23 -0.0069569648686140795 1107 24-25 -0.3337562409764345 1107 26-27 -0.45930304321654347 1107 28-29 -0.6942613411622105 1107 30-31 -0.8672076512938176 1107 32-33 -0.6410097591208981 1107 34-35 -0.8467500139011079 1107 36-37 -0.481092273670356 1107 38-39 -0.4665113905703677 1107 40-41 -0.7712164564570259 1107 42-43 -1.4262783780253372 1107 44-45 -1.569705601422612 1107 46-47 -1.4479153716942434 1107 48-49 -1.2390283367190662 1107 50-51 -1.2364693621451721 1107 52-53 -0.7839996220536278 1107 54-55 -1.3480706130074793 1107 56-57 -1.3145971001297987 1107 58-59 -0.8954107823492627 1107 60-61 -0.8314205284148457 1107 62-63 -0.9982896676515196 1107 64-65 -0.6295025598746236 1107 66-67 -0.33471769534776996 1107 68-69 0.07277509850154829 1107 70-71 -0.09017503114650083 1107 72-73 -0.1525769357406226 1107 74-75 -0.3132199907060276 1107 76-77 -0.07478664395269163 1107 78-79 -0.056849548938348704 1107 80-81 0.1204184338743417 1107 82-83 -0.1519905063406526 1107 84-85 -0.20258169015792404 1107 86-87 -0.2121807842252288 1107 88-89 -0.1512946045043293 1107 90-91 0.024893907577908436 1107 92-93 -0.37085672042794826 1107 94-95 -0.26239934310057933 1107 96-97 -0.15207870039020932 1107 98-99 0.2181283403619041 1107 100-101 0.4743007279598217 1108 1 0.2717489573552072 1108 2 0.0033117921469738576 1108 3 0.2411716381042126 1108 4 0.19913369106146206 1108 5 0.05797826113442994 1108 6 0.04208600978223842 1108 7 0.31710739366662466 1108 8 0.20847947343764872 1108 9 0.2806357306202756 1108 10-11 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-1.468789220037202 2316 88-89 -0.8674191878855524 2316 90-91 -0.7604755901809703 2316 92-93 -0.6412854067858369 2316 94-95 -0.7011994810157631 2316 96-97 -0.8345577257895904 2316 98-99 -0.5515820377555514 2316 100-101 -0.4902148449628321 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 2.0 5 19.0 6 35.0 7 65.0 8 124.0 9 190.0 10 313.0 11 519.0 12 841.0 13 1214.0 14 1552.0 15 2287.0 16 3164.0 17 4508.0 18 4124.0 19 2219.0 20 1843.0 21 1919.0 22 1931.0 23 2137.0 24 2212.0 25 2334.0 26 2603.0 27 2881.0 28 3402.0 29 3825.0 30 4607.0 31 5629.0 32 7226.0 33 9763.0 34 15484.0 35 27228.0 36 23335.0 37 23937.0 38 18671.0 39 1847.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.31234808897975 23.32700767235614 13.574795956652002 11.785848282012104 2 15.082177485977653 29.74966302882734 37.482607939475635 17.68555154571938 3 22.258032957954693 38.758750380451325 26.89410409148224 12.089112570111745 4 10.409691725727207 22.16944214965868 35.67926866385495 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40.01777251184834 30.971998782555765 12.531794860646114 26-27 16.381957617031453 39.49393713754626 31.965497937376693 12.158607308045589 28-29 14.074907466288378 41.985303309472954 31.651964540973005 12.287824683265665 30-31 14.276979433888428 43.6162985347189 30.1765837645115 11.930138266881169 32-33 13.897071611809208 43.992130092612726 29.618407322057482 12.492390973520589 34-35 14.158224270620462 43.3815057176399 29.718683421018305 12.741586590721335 36-37 14.649821731379625 43.98234705856776 29.987988608200354 11.379842601852253 38-39 14.762054871950955 44.164963694073656 29.665963737553806 11.407017696421583 40-41 12.961433105787206 45.41039827818601 29.60210226531588 12.0260663507109 42-43 12.988424837542773 46.46018540915305 27.762995898821284 12.788393854482896 44-45 13.700867429018654 46.197660767859475 26.900626114178877 13.200845688942998 46-47 13.972890125657637 46.362613591895304 25.86905952432714 13.79543675811992 48-49 14.596286795078047 46.74523892343145 25.582090525675028 13.07638375581547 50-51 14.521011783121004 47.48711900517414 25.316861602678376 12.675007609026478 52-53 13.787012478803426 48.072470542197486 24.990488716900735 13.15002826209835 54-55 12.744032349232576 47.75044567155094 24.69373668420366 14.811785295012827 56-57 14.266109396060697 48.29150180442628 23.305904604548026 14.136484194964998 58-59 14.653354493673637 47.2903713204922 23.71923779294752 14.337036392886649 60-61 13.876962041827905 47.64500630462194 24.670366102874038 13.807665550676115 62-63 15.042230096960738 46.99198878212096 24.58775381538328 13.378027305535023 64-65 14.361765728944736 47.760500456541585 24.574438019044305 13.303295795469369 66-67 13.910930910039568 46.853395799817385 24.470900908735164 14.764772381407887 68-69 13.653311013522327 47.96512891864864 23.948323840166964 14.433236227662071 70-71 14.375353276229403 47.49581503543632 23.2379668681247 14.890864820209574 72-73 14.031588329927388 46.489249532588374 23.806469846515064 15.672692290969174 74-75 14.004684986303753 47.8246336797252 23.325742423583634 14.844938910387409 76-77 14.190562633157963 46.05852428366451 24.623624940214793 15.127288142962739 78-79 13.206280707856862 46.334623244488895 24.917115961563546 15.541980086090701 80-81 13.151387016826819 47.28928431670943 24.223607548154266 15.335721118309491 82-83 13.02176181573112 46.59930866559416 23.915441975738077 16.46348754293665 84-85 12.944312796208532 46.04738249489108 23.88120135658072 17.12710335231967 86-87 13.399767381190486 46.52729466498543 23.564339753902345 16.508598199921735 88-89 13.515261533110134 45.641658332971 23.859189529979563 16.983890603939304 90-91 12.73515423291208 45.73719255333605 24.230737871993476 17.29691534175839 92-93 12.27273962551295 46.97475337663396 23.27825637959616 17.474250618256924 94-95 12.063024479325188 46.781925301100046 22.7439236488543 18.411126570720466 96-97 12.133951476151136 46.26940301752251 22.7235423279273 18.873103178399063 98-99 13.322082813608239 46.90189492449379 21.714590076171998 18.06143218572597 100-101 12.96887697831363 46.82015725945636 21.76600249506016 18.444963267169847 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6344.0 1 3678.0 2 1140.5 3 1493.5 4 1709.0 5 1910.0 6 2788.0 7 4178.0 8 4578.0 9 3950.0 10 3431.0 11 3411.5 12 3620.0 13 3532.5 14 3163.5 15 2580.5 16 2002.0 17 1682.0 18 1523.0 19 1420.0 20 1389.5 21 1395.5 22 1446.0 23 1541.0 24 1679.5 25 1841.0 26 2021.5 27 2292.0 28 2531.0 29 2633.5 30 2830.0 31 3081.5 32 3498.0 33 4023.0 34 4460.5 35 4698.5 36 4985.5 37 5303.0 38 5100.0 39 4696.5 40 4473.0 41 4164.5 42 3745.5 43 3588.0 44 3492.0 45 3153.5 46 3097.5 47 3986.5 48 8593.5 49 8453.5 50 4330.5 51 3175.5 52 1769.5 53 1266.0 54 1057.5 55 939.5 56 959.5 57 1037.0 58 916.5 59 941.0 60 1066.5 61 1120.5 62 1012.0 63 521.5 64 168.5 65 110.5 66 89.0 67 76.5 68 57.0 69 35.5 70 24.5 71 28.0 72 33.0 73 25.0 74 18.5 75 17.0 76 14.0 77 9.5 78 5.5 79 3.5 80 3.0 81 1.0 82 0.5 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04619766076785947 2 0.0 3 0.0 4 0.0 5 0.022283577546849863 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004891517022479238 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.001630505674159746 28-29 0.00271750945693291 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.011141788773424931 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.009239532153571895 92-93 0.0038045132397060743 94-95 0.0 96-97 0.0 98-99 2.71750945693291E-4 100-101 0.01548980390451759 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 183992.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.30540458280795 #Duplication Level Percentage of deduplicated Percentage of total 1 81.62988602041492 40.24794556285055 2 10.189818999537028 10.048262967955129 3 3.169161577636191 4.68770381320927 4 1.4010449965828171 2.763163615809383 5 0.8278401199320973 2.040849602156616 6 0.48722414515311185 1.4413670159572156 7 0.3747878039639322 1.2935345015000652 8 0.25904451156330605 1.0217835558067743 9 0.2061332921801627 0.9147136832036176 >10 1.2687669481249588 11.493977999043437 >50 0.10472012169580457 3.4968911691812687 >100 0.06393439008796489 5.771990086525501 >500 0.009920853634339382 3.035458063394061 >1k 0.006613902422892921 7.259554763250577 >5k 0.0011023170704821533 4.482803600156529 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8248 4.482803600156529 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3312 1.8000782642723596 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2689 1.461476585938519 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2166 1.1772250967433369 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2033 1.1049393451889213 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1690 0.9185181964433237 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1467 0.7973172746641157 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 721 0.3918648636897257 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 711 0.38642984477585984 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 678 0.3684942823601026 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 670 0.36414626722900995 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 622 0.338058176442454 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 578 0.31414409322144443 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 545 0.2962085308056872 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 542 0.2945780251315274 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 518 0.28153397973824945 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 454 0.24674985868950822 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 369 0.20055219792164874 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 356 0.19348667333362318 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 313 0.17011609200400019 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 307 0.16685508065568067 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 301 0.1635940693073612 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 276 0.15000652202269663 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 274 0.14891951823992347 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 274 0.14891951823992347 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 274 0.14891951823992347 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 261 0.1418539936518979 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 255 0.13859298230357842 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 235 0.12772294447584678 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 231 0.12554893691030045 No Hit GTACATGGGAATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 227 0.12337492934475411 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 219 0.11902691421366146 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 214 0.11630940475672856 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 202 0.10978738206008958 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 194 0.10543936692899691 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 188 0.10217835558067742 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 8.15252837079873E-4 0.0 20-21 0.0 0.0 0.0 0.001630505674159746 0.0 22-23 0.0 0.0 0.0 0.003261011348319492 0.0 24-25 0.0 0.0 0.0 0.004891517022479238 0.0 26-27 0.0 0.0 0.0 0.006250271750945694 0.0 28-29 0.0 0.0 0.0 0.011413539719118224 0.0 30-31 0.0 0.0 0.0 0.019294317144223663 0.0 32-33 0.0 0.0 0.0 0.025544588895169355 0.0 34-35 0.0 0.0 0.0 0.03206661159180834 0.0 36-37 0.0 0.0 0.0 0.039947389016913776 0.0 38-39 0.0 0.0 0.0 0.05951345710683073 0.0 40-41 0.0 0.0 0.0 0.09701508761250488 0.0 42-43 0.0 0.0 0.0 0.15707204661072222 0.0 44-45 0.0 0.0 0.0 0.2092482281838341 0.0 46-47 0.0 0.0 0.0 0.32664463672333577 0.0 48-49 0.0 0.0 0.0 0.4598026001130484 0.0 50-51 0.0 0.0 0.0 0.5600786990738728 0.0 52-53 0.0 0.0 0.0 0.6587242923605374 0.0 54-55 0.0 0.0 0.0 0.7413365798512979 0.0 56-57 0.0 0.0 0.0 0.9060176529414322 0.0 58-59 0.0 0.0 0.0 1.1095591112657073 0.0 60-61 0.0 0.0 0.0 1.3421779207791644 0.0 62-63 0.0 0.0 0.0 1.6019718248619506 0.0 64-65 0.0 0.0 0.0 1.827525109787382 0.0 66-67 0.0 0.0 0.0 2.131070916126788 0.0 68-69 0.0 0.0 0.0 2.4343449715205008 0.0 70-71 0.0 0.0 0.0 2.712889690856124 0.0 72-73 0.0 0.0 0.0 3.0061089612591854 0.0 74-75 0.0 0.0 0.0 3.3197095525892433 0.0 76-77 0.0 0.0 0.0 3.660756989434323 0.0 78-79 0.0 0.0 0.0 4.02381625288056 0.0 80-81 0.0 0.0 0.0 4.440953954519761 0.0 82-83 0.0 0.0 0.0 4.829286055915475 0.0 84-85 0.0 0.0 0.0 5.402408800382625 0.0 86-87 0.0 0.0 0.0 5.847265098482543 0.0 88-89 0.0 0.0 0.0 6.470661767902952 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCTAG 20 0.002071394 71.24918 6 AGTGACC 25 0.005014818 56.999348 8 CGCTAAT 20 5.383679E-4 47.499454 82-83 CTCGCTA 20 5.383679E-4 47.499454 80-81 GGGGGAT 60 8.264162E-5 39.582882 7 CGTCATC 25 0.0016144786 37.999565 40-41 AGGAGCC 80 1.0727834E-5 35.62459 7 GGATTGT 55 0.002574164 34.545063 8 GGGAGAT 70 2.0467959E-4 33.92818 7 ATGGGGG 280 0.0 32.231773 5 ATCTCGC 30 0.0039477125 31.666306 78-79 GGGAGCA 60 0.0039426256 31.666306 7 GGAGTCT 75 3.0686843E-4 31.666306 8 GGGATAC 60 0.0039426256 31.666306 7 GGGGGAC 90 2.4022296E-5 31.666302 7 ATGGGAG 770 0.0 30.226927 5 TCTATAT 80 4.4794416E-4 29.68716 3 GGGAGTC 65 0.005831813 29.230433 7 GGAACAT 65 0.005831813 29.230433 8 AGTACTC 65 0.005831813 29.230433 5 >>END_MODULE