##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937835_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 211683 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.805454382260265 33.0 31.0 34.0 30.0 34.0 2 31.911509190629385 33.0 31.0 34.0 30.0 34.0 3 31.878181053745458 33.0 31.0 34.0 30.0 34.0 4 35.49045506724678 37.0 35.0 37.0 33.0 37.0 5 35.48463031986508 37.0 35.0 37.0 33.0 37.0 6 35.593122735410965 37.0 35.0 37.0 33.0 37.0 7 35.56063547852213 37.0 35.0 37.0 33.0 37.0 8 35.57810972066722 37.0 35.0 37.0 33.0 37.0 9 37.1906907970881 39.0 37.0 39.0 34.0 39.0 10-11 37.112914121587465 39.0 37.0 39.0 34.0 39.0 12-13 37.019552821908235 39.0 37.0 39.0 33.5 39.0 14-15 38.09286763698549 40.0 38.0 41.0 33.0 41.0 16-17 38.027635662759884 40.0 38.0 41.0 33.0 41.0 18-19 38.06337778659599 40.0 38.0 41.0 33.5 41.0 20-21 38.04703967725325 40.0 38.0 41.0 34.0 41.0 22-23 37.93634821879886 40.0 38.0 41.0 33.0 41.0 24-25 37.90813386053675 40.0 38.0 41.0 33.0 41.0 26-27 37.76125858004658 40.0 37.0 41.0 33.0 41.0 28-29 37.59784678032719 40.0 37.0 41.0 33.0 41.0 30-31 37.22652031575516 39.0 36.5 41.0 33.0 41.0 32-33 36.84833217594233 39.0 35.0 41.0 32.0 41.0 34-35 36.66628401902845 39.0 35.0 41.0 31.5 41.0 36-37 36.45547823868709 39.0 35.0 41.0 31.0 41.0 38-39 36.22403783015169 39.0 35.0 41.0 30.5 41.0 40-41 35.894020776349535 39.0 35.0 41.0 30.0 41.0 42-43 35.46276980201527 39.0 35.0 41.0 28.0 41.0 44-45 34.97288162015845 38.5 35.0 41.0 25.0 41.0 46-47 34.31924150734825 38.0 35.0 40.0 21.0 41.0 48-49 33.763589896212736 38.0 34.0 40.0 11.0 41.0 50-51 32.75540785041784 37.5 33.0 39.5 4.0 40.5 52-53 32.79727233646537 38.0 33.0 39.5 2.0 40.5 54-55 33.03730342068093 38.0 33.0 40.0 2.0 41.0 56-57 32.765344878899114 38.0 33.0 40.0 2.0 41.0 58-59 32.5314574150971 38.0 33.0 40.0 2.0 41.0 60-61 32.21407481942339 38.0 33.0 40.0 2.0 41.0 62-63 31.869588016042854 37.0 32.0 40.0 2.0 41.0 64-65 31.497997004955522 36.5 31.5 40.0 2.0 41.0 66-67 31.067286933764166 36.0 31.0 39.0 2.0 41.0 68-69 30.584730469617305 35.5 30.5 39.0 2.0 40.5 70-71 30.124896661517457 35.0 29.5 39.0 2.0 40.0 72-73 29.675349461222677 35.0 29.0 38.0 2.0 40.0 74-75 29.183434664096787 35.0 28.5 37.5 2.0 39.5 76-77 28.69821383861718 35.0 28.0 37.0 2.0 39.0 78-79 28.166917041047228 34.0 26.5 36.0 2.0 39.0 80-81 27.635393489321295 34.0 26.0 36.0 2.0 38.5 82-83 27.142829136019426 34.0 25.5 35.0 2.0 37.0 84-85 26.633657402814585 33.5 24.5 35.0 2.0 37.0 86-87 26.15803111255982 33.0 22.0 35.0 2.0 36.0 88-89 25.74304266284964 33.0 20.0 35.0 2.0 36.0 90-91 25.258431239164224 33.0 18.0 35.0 2.0 35.5 92-93 24.708089454514536 32.0 9.0 35.0 2.0 35.0 94-95 24.08675944690882 32.0 2.0 35.0 2.0 35.0 96-97 23.39707487138788 31.0 2.0 34.0 2.0 35.0 98-99 22.56103702234001 31.0 2.0 34.0 2.0 35.0 100-101 20.147817727450953 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6980369339484049 1101 2 0.5976266155361181 1101 3 0.6300807180462549 1101 4 0.3144031646155767 1101 5 0.25464494758276146 1101 6 0.3262961553856769 1101 7 0.33703561241311064 1101 8 0.370346823019986 1101 9 0.4848409680275978 1101 10-11 0.5059944893181267 1101 12-13 0.23020780221560955 1101 14-15 0.8209125483980202 1101 16-17 0.3838667507331692 1101 18-19 0.46654998682124926 1101 20-21 0.37700444103583663 1101 22-23 0.4898722296948037 1101 24-25 0.7386944636282706 1101 26-27 0.34135520614943715 1101 28-29 0.49126648373102455 1101 30-31 0.6984804904672259 1101 32-33 0.5179321587535881 1101 34-35 0.6592214837594881 1101 36-37 0.8066171982515442 1101 38-39 0.6011607686157063 1101 40-41 0.7592890682472273 1101 42-43 1.5009166446760815 1101 44-45 1.7809643176058287 1101 46-47 1.700009800156927 1101 48-49 1.458082259439557 1101 50-51 1.9617267032325856 1101 52-53 1.9765796803999436 1101 54-55 1.8713681392837458 1101 56-57 1.7499438783568948 1101 58-59 1.4267344730158769 1101 60-61 1.4347920276860897 1101 62-63 1.4830395856066083 1101 64-65 1.637649400136855 1101 66-67 1.5162503451655631 1101 68-69 1.4306753900387434 1101 70-71 1.110958973942985 1101 72-73 1.2923761897566806 1101 74-75 1.2844608040328183 1101 76-77 0.954559764797704 1101 78-79 0.9856013270113273 1101 80-81 1.23147447837653 1101 82-83 1.4656646839922658 1101 84-85 1.7145280439469701 1101 86-87 1.8896403078796737 1101 88-89 1.884814440950656 1101 90-91 1.5111086674081236 1101 92-93 1.5692609784457616 1101 94-95 1.5107275727646332 1101 96-97 1.5019768086906318 1101 98-99 1.6867568396612818 1101 100-101 2.204701780738059 1102 1 0.4128659690107064 1102 2 0.5813632136236286 1102 3 0.6394620567886484 1102 4 0.5382151031841076 1102 5 0.22421898409392327 1102 6 0.32901275926861473 1102 7 0.4322978552412735 1102 8 0.26736942516428996 1102 9 0.4466636281261245 1102 10-11 0.4947658599353417 1102 12-13 0.595971349013638 1102 14-15 0.7220281670592854 1102 16-17 0.8482792398667556 1102 18-19 0.6147860053666037 1102 20-21 0.5951839542204169 1102 22-23 0.4770679700599132 1102 24-25 0.5063886102524364 1102 26-27 -0.062078581158587554 1102 28-29 0.35427720526102036 1102 30-31 0.46548643077438356 1102 32-33 0.7152481541172477 1102 34-35 0.9410600812674588 1102 36-37 0.875745711725898 1102 38-39 1.063182646332315 1102 40-41 0.7897150317360726 1102 42-43 0.8503443467914167 1102 44-45 0.6831303564351572 1102 46-47 0.8045628282647215 1102 48-49 0.8210386488918857 1102 50-51 0.4631755586368058 1102 52-53 0.18012575400179998 1102 54-55 0.10715124562973699 1102 56-57 -0.013747805213078834 1102 58-59 0.13665551039634494 1102 60-61 0.37432042525201936 1102 62-63 0.1901896870264892 1102 64-65 -0.30280264274926694 1102 66-67 -0.060955537187378894 1102 68-69 -0.14711003447589377 1102 70-71 0.04831408840255946 1102 72-73 0.15076868468568705 1102 74-75 0.428567584676113 1102 76-77 0.4359419728533389 1102 78-79 0.18702844958449916 1102 80-81 0.2855892277245431 1102 82-83 0.8350684799934243 1102 84-85 1.2390137002784662 1102 86-87 0.9694179085751244 1102 88-89 0.636951502671895 1102 90-91 0.8364129270430105 1102 92-93 1.1734336820099465 1102 94-95 1.2639331653464936 1102 96-97 1.4845543224547555 1102 98-99 0.8986338318374649 1102 100-101 0.9837875529779865 1103 1 0.0862553247530009 1103 2 -0.08971054921484267 1103 3 -0.1289039562832457 1103 4 -0.25216771661047943 1103 5 -0.2922516041413772 1103 6 -0.610389668369109 1103 7 -0.23533986651409577 1103 8 -0.38775662525588217 1103 9 -0.31619351473101887 1103 10-11 -0.38385225397689027 1103 12-13 -0.2622826192403309 1103 14-15 -0.1859456891498681 1103 16-17 0.0766732641431318 1103 18-19 0.06768983356455038 1103 20-21 -0.14743042486454527 1103 22-23 -0.019141180266878166 1103 24-25 -0.008326609168669563 1103 26-27 -0.19596280151339585 1103 28-29 -0.3881971178015391 1103 30-31 0.10416056709557608 1103 32-33 0.25257775262331705 1103 34-35 -0.04451414842441892 1103 36-37 -0.07461843393235768 1103 38-39 0.23802858470765642 1103 40-41 0.1761856199713634 1103 42-43 0.10821549525079632 1103 44-45 -0.04867169771983981 1103 46-47 0.07061324843279237 1103 48-49 0.1342412632709724 1103 50-51 0.22697574537816934 1103 52-53 0.3039072666068421 1103 54-55 0.5807646910078859 1103 56-57 0.24350709674769888 1103 58-59 0.33026895577449267 1103 60-61 0.38719624224547644 1103 62-63 0.23110471970622726 1103 64-65 0.3297183656540916 1103 66-67 -0.05180521038998975 1103 68-69 -0.14592422681949557 1103 70-71 -6.214858271285095E-4 1103 72-73 -0.2658326225038543 1103 74-75 0.2551408806611697 1103 76-77 -0.1572793156620662 1103 78-79 -0.15223392203081332 1103 80-81 0.47247064695890373 1103 82-83 0.46090414759379783 1103 84-85 0.6269035228741728 1103 86-87 0.3152629132436573 1103 88-89 0.5151401114487406 1103 90-91 0.25151096625869584 1103 92-93 0.05255599760285179 1103 94-95 0.1734383007339808 1103 96-97 0.929773743556531 1103 98-99 1.1067664182053427 1103 100-101 0.8028818573664083 1104 1 0.6274833949284364 1104 2 0.5928625502003619 1104 3 0.43203171623137493 1104 4 0.39403111379888145 1104 5 0.7100290976130097 1104 6 0.5612477585314721 1104 7 0.528051946351404 1104 8 0.45224337474412124 1104 9 0.5757363098303827 1104 10-11 0.8698027167833402 1104 12-13 0.7936530532742907 1104 14-15 0.5673584044173836 1104 16-17 0.5467902232074593 1104 18-19 0.7281576698218615 1104 20-21 0.9371602184103125 1104 22-23 0.672759404528442 1104 24-25 0.7585447358605748 1104 26-27 0.6520781341591189 1104 28-29 0.7415689640698062 1104 30-31 0.5484880524756903 1104 32-33 0.46979997484553593 1104 34-35 0.6151349743825989 1104 36-37 0.4916973555413193 1104 38-39 0.7745198127778323 1104 40-41 0.6175014094450475 1104 42-43 0.7058470741981608 1104 44-45 0.6699540332743155 1104 46-47 0.7551746519415587 1104 48-49 0.32903658490840115 1104 50-51 -5.388745048691135E-4 1104 52-53 0.13610024906297724 1104 54-55 0.28988749802543623 1104 56-57 0.6147351669231469 1104 58-59 1.021146148756948 1104 60-61 0.7553833767484015 1104 62-63 0.7438532577179195 1104 64-65 0.8352446814435659 1104 66-67 0.9120836784988988 1104 68-69 1.038169340431967 1104 70-71 1.4023609703132216 1104 72-73 1.2169451552739226 1104 74-75 1.2090297695500603 1104 76-77 1.2577206843379365 1104 78-79 1.4492280662732746 1104 80-81 1.2617981323390204 1104 82-83 1.4235064867750822 1104 84-85 1.405090657377098 1104 86-87 1.652836012658863 1104 88-89 1.5024792927352912 1104 90-91 1.5244934223990505 1104 92-93 1.3549536584215645 1104 94-95 1.409812569739831 1104 96-97 1.069656784492203 1104 98-99 1.1328775293164561 1104 100-101 1.8014198690623147 1105 1 0.23697996243415886 1105 2 0.26265660054361106 1105 3 0.1601781693206199 1105 4 -0.09178124318052738 1105 5 0.10861796107601407 1105 6 0.2862286891188148 1105 7 0.08823501271295697 1105 8 0.2870259834397757 1105 9 0.2820673548341972 1105 10-11 0.45044726293131987 1105 12-13 0.14317631795773877 1105 14-15 0.17241179877283486 1105 16-17 0.265707080568248 1105 18-19 0.15599901230851287 1105 20-21 0.22855991329970493 1105 22-23 0.2066076120036584 1105 24-25 0.29853329421298014 1105 26-27 0.40080048351075703 1105 28-29 0.3218511913771991 1105 30-31 0.43609293424533035 1105 32-33 0.381031858903512 1105 34-35 0.7656307791118167 1105 36-37 0.7190047544734313 1105 38-39 0.788173512243894 1105 40-41 0.39227257649309877 1105 42-43 0.503577814091372 1105 44-45 0.36398530711107924 1105 46-47 0.05017846582409646 1105 48-49 -0.33740124880632294 1105 50-51 -0.10109188747206588 1105 52-53 -0.12261447252359403 1105 54-55 -0.34619183073124304 1105 56-57 -0.5170726133972252 1105 58-59 -1.1492962616168079 1105 60-61 -1.1965235645178076 1105 62-63 -1.005078855173 1105 64-65 -0.8524555473893862 1105 66-67 -0.6875540026605229 1105 68-69 -0.7475667388967864 1105 70-71 -1.0217809061169838 1105 72-73 -0.823272236030423 1105 74-75 -0.7551006652325505 1105 76-77 -1.244090909864962 1105 78-79 -1.069383680484922 1105 80-81 -1.0067564061811929 1105 82-83 -1.3334919876719056 1105 84-85 -0.8736278780920124 1105 86-87 -0.849471386273251 1105 88-89 -0.7253429803386986 1105 90-91 -0.7953006279441972 1105 92-93 -0.7413087366966664 1105 94-95 -0.9316824722128771 1105 96-97 -1.2209992033033714 1105 98-99 -1.1790789924226743 1105 100-101 -0.5777944711360057 1106 1 -0.01940505260548875 1106 2 0.14433557237844852 1106 3 0.3449744504253509 1106 4 0.31806289135597865 1106 5 0.23328298705359174 1106 6 0.276822071672818 1106 7 0.3799221879932446 1106 8 0.25098551310890116 1106 9 0.5773913909293569 1106 10-11 -0.12179774663935916 1106 12-13 -0.38538534460720086 1106 14-15 0.28529120812476094 1106 16-17 0.17034202724585157 1106 18-19 0.12173595515784541 1106 20-21 -0.0015604039001857473 1106 22-23 0.17851081134737257 1106 24-25 0.11351825456299025 1106 26-27 0.3243306995348192 1106 28-29 0.21930812328860583 1106 30-31 0.5868859339718853 1106 32-33 0.31492576100906433 1106 34-35 0.4913349081793612 1106 36-37 0.5314193019166993 1106 38-39 0.6528713520032525 1106 40-41 0.6470032300342581 1106 42-43 0.6728695833011074 1106 44-45 1.0260033546910634 1106 46-47 1.043820795602599 1106 48-49 0.8191888523694999 1106 50-51 0.505047024204174 1106 52-53 0.7864229898772805 1106 54-55 0.939506829372668 1106 56-57 0.6580982917162572 1106 58-59 0.2677532325040559 1106 60-61 0.793946766354491 1106 62-63 0.4389873192869409 1106 64-65 0.42267434049685804 1106 66-67 0.26035411875046677 1106 68-69 0.18480952580105026 1106 70-71 -0.09722525941203841 1106 72-73 -0.48667119692733607 1106 74-75 -0.5354670857958546 1106 76-77 -0.13300258610231808 1106 78-79 0.13120423310545348 1106 80-81 0.5148186553446479 1106 82-83 0.30421651656654447 1106 84-85 0.2722703991844462 1106 86-87 0.15949771408223157 1106 88-89 0.49631411564161354 1106 90-91 0.34408958261090206 1106 92-93 0.028488911648970827 1106 94-95 0.10643620429792477 1106 96-97 0.372205609384622 1106 98-99 0.9025316173667726 1106 100-101 1.1571795512867418 1107 1 0.3995886580863335 1107 2 0.5591783396740446 1107 3 0.25887382149453586 1107 4 -0.244389938832704 1107 5 0.20774839585862281 1107 6 0.40405477607533413 1107 7 0.05910457793034851 1107 8 0.4322433747441181 1107 9 0.3688064852689763 1107 10-11 0.4739255238008866 1107 12-13 0.4338284918707842 1107 14-15 0.26599875529457506 1107 16-17 0.6647288196986807 1107 18-19 0.7449120557867701 1107 20-21 0.6206251306910104 1107 22-23 0.6653032641775596 1107 24-25 0.574728946386891 1107 26-27 0.15014830959771075 1107 28-29 -0.12097489557931596 1107 30-31 0.2055494559844604 1107 32-33 0.09646664151220818 1107 34-35 0.007152518242243389 1107 36-37 -0.27045176726569764 1107 38-39 -0.627804748625671 1107 40-41 -0.8488143800286352 1107 42-43 -1.0751178380825337 1107 44-45 -1.3139494754976155 1107 46-47 -1.48938675156721 1107 48-49 -1.3713142922845876 1107 50-51 -1.8266353657329404 1107 52-53 -1.446092733393158 1107 54-55 -0.826735308992113 1107 56-57 -1.260659569918964 1107 58-59 -1.339731044225502 1107 60-61 -1.1914148688656319 1107 62-63 -0.8008397247382177 1107 64-65 -0.4252816343459038 1107 66-67 -0.5262496548344373 1107 68-69 -0.7639797823750492 1107 70-71 -0.8289548191604617 1107 72-73 -1.0430548447260755 1107 74-75 -0.73597023044994 1107 76-77 -1.1481126489953972 1107 78-79 -1.2111228109197079 1107 80-81 -0.5744737974855418 1107 82-83 -0.8013180746284263 1107 84-85 -0.5750409215702739 1107 86-87 -0.7400148645341176 1107 88-89 -0.9595821107734857 1107 90-91 -0.6143223670746352 1107 92-93 -0.34022178017492877 1107 94-95 -0.11961725482157348 1107 96-97 0.12171818800097611 1107 98-99 0.5253775293164544 1107 100-101 1.1942707462552988 1108 1 0.16340888280543453 1108 2 0.20344800259539753 1108 3 0.2475255068877935 1108 4 0.09302579150438106 1108 5 0.457860755409186 1108 6 0.4677626412438727 1108 7 0.1962955891663043 1108 8 0.2612321387890617 1108 9 0.23077277740380708 1108 10-11 0.1635884451492018 1108 12-13 0.05062624467976917 1108 14-15 0.20467853057547103 1108 16-17 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2316 70-71 -0.9924382314827405 2316 72-73 -1.133173328138401 2316 74-75 -0.8780555385068105 2316 76-77 -1.4387643077631722 2316 78-79 -1.1164782611566686 2316 80-81 -0.5188932287651618 2316 82-83 -1.0069104916900358 2316 84-85 -1.9425646182527352 2316 86-87 -1.3814722105056845 2316 88-89 -1.3570347174085562 2316 90-91 -0.6368934571220279 2316 92-93 -0.9012407375209008 2316 94-95 -0.5493210462907676 2316 96-97 -0.49440740441608355 2316 98-99 -0.9340655986456277 2316 100-101 -1.080741102085934 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1159.0 3 200.0 4 165.0 5 175.0 6 193.0 7 220.0 8 298.0 9 333.0 10 513.0 11 710.0 12 1155.0 13 1603.0 14 1888.0 15 2658.0 16 3448.0 17 4669.0 18 3862.0 19 2443.0 20 2216.0 21 2046.0 22 2076.0 23 2173.0 24 2369.0 25 2552.0 26 2780.0 27 3160.0 28 3540.0 29 4147.0 30 4912.0 31 6018.0 32 7460.0 33 9509.0 34 13172.0 35 19010.0 36 24344.0 37 36369.0 38 33778.0 39 4360.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.19836559792192 20.53620522932823 17.092963728408638 19.172465444341213 2 14.587953223024583 21.785030609730672 42.04526070392904 21.58175546331571 3 18.33050630632338 26.34773379829241 34.36164466639502 20.96011522898919 4 11.099159886847703 18.94542818288216 40.63153006733225 29.323881862937885 5 10.585876967258562 37.71165356345697 37.298416715240954 14.404052754043514 6 25.914600955189403 38.48731699697539 19.465889135548856 16.132192912286346 7 22.00096559819755 36.626464963932065 23.973815248878203 17.39875418899218 8 23.089193078117308 33.27795448521337 23.071680109053734 20.561172327615584 9 24.14930728123861 16.828782263370396 24.88107578489982 34.140834670491174 10-11 22.017603858743115 27.081730848880888 29.989988663285377 20.910676629090624 12-13 24.560950963292875 26.487773362899087 30.38874860671004 18.562527067098006 14-15 20.955087956794316 27.59095653161735 32.13564729242646 19.31830821916187 16-17 16.918489065606362 30.193836978131213 33.15772034459907 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27.826303023131988 37.234100455540435 18.264213453233154 44-45 17.37040648251576 27.354266457146103 36.88800431662849 18.38732274370965 46-47 17.947862307264487 27.55511377755689 36.08079804039902 18.416225874779606 48-49 17.751806989392367 27.569427680190095 35.80750060351316 18.87126472690438 50-51 17.236180309826153 28.151575758292996 35.890602255648 18.721641676232856 52-53 17.850107958634798 28.562398196901395 34.59150914807379 18.995984696390014 54-55 17.666073490714275 27.463415557616948 35.28576567025728 19.584745281411507 56-57 18.318463260154118 27.84122500065064 34.115520476220894 19.724791262974346 58-59 18.689357715248317 28.03320848997901 34.24122764471932 19.036206150053353 60-61 18.332300296662897 27.26625376742954 35.51272527690903 18.888720658998537 62-63 17.953079678428292 27.457804359861264 35.27825227007103 19.31086369163942 64-65 17.82552533086026 27.909370076132163 34.994061785817365 19.27104280719021 66-67 18.223349413153592 27.403278579928692 34.46530851725761 19.9080634896601 68-69 18.146994035154965 26.868065956374526 35.32697808788978 19.65796192058073 70-71 18.699502447541693 26.859788628927127 34.70469800245583 19.736010921075355 72-73 18.000208193354847 26.318242469551723 35.78063044733181 19.900918889761616 74-75 17.695088654076386 27.292063957752678 35.153340557524594 19.859506830646346 76-77 17.638025559543895 26.603505268313686 35.18093043175801 20.57753874038441 78-79 17.487364176731155 26.36296520652709 35.420768182334456 20.7289024344073 80-81 17.96597738134671 26.196469975867124 34.902522121800025 20.935030520986135 82-83 17.623692571939596 26.1301075752163 35.64106264535194 20.605137207492174 84-85 17.657342657342657 25.99099141724311 35.00170330346244 21.349962621951796 86-87 17.415012656431124 26.673132880698354 34.69801991909347 21.213834543777057 88-89 17.739481821644628 25.646539996640428 34.627951158460554 21.986027023254383 90-91 17.527795326078984 25.141313117690345 35.32271917433862 22.008172381892045 92-93 17.49758696392748 25.234916158825087 35.81986449146448 21.44763238578296 94-95 17.222737467705148 25.286749884069764 35.43324784464402 22.057264803581063 96-97 16.676839799187096 25.41768989453064 35.811792316682514 22.093677989599747 98-99 17.499136376757416 25.463398937151887 35.01791130944213 22.019553376648574 100-101 17.26703960829302 24.843352690028738 35.78631154423852 22.10329615743971 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1595.0 1 1062.5 2 622.5 3 715.0 4 541.5 5 437.0 6 749.0 7 1408.0 8 2048.0 9 2198.5 10 1597.0 11 912.0 12 740.5 13 709.0 14 697.0 15 694.0 16 702.0 17 749.5 18 821.0 19 907.5 20 1034.5 21 1164.5 22 1239.0 23 1439.0 24 1734.0 25 2061.5 26 2522.0 27 3122.5 28 3561.0 29 4052.0 30 4673.0 31 5133.5 32 5603.0 33 6103.0 34 6814.0 35 7374.5 36 7639.5 37 7900.5 38 7740.5 39 7258.0 40 6904.5 41 6589.0 42 6321.5 43 6200.0 44 6123.0 45 6002.5 46 6113.5 47 7080.5 48 9106.0 49 8705.0 50 6092.0 51 4600.5 52 3805.5 53 3331.5 54 2934.0 55 2526.5 56 2094.0 57 1762.5 58 1603.0 59 1400.0 60 1172.0 61 1006.5 62 831.0 63 574.5 64 370.0 65 287.5 66 223.5 67 186.0 68 131.5 69 79.5 70 56.0 71 40.5 72 28.0 73 23.0 74 19.5 75 13.5 76 11.0 77 10.5 78 10.5 79 9.0 80 8.0 81 4.5 82 1.0 83 0.5 84 2.0 85 2.0 86 1.0 87 1.5 88 1.5 89 1.0 90 0.0 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33965882947615067 2 0.3018664701463981 3 0.29525280726369146 4 0.30233887463802006 5 0.31462139142018963 6 0.19652026851471305 7 0.1960478640230911 8 0.1941582460566035 9 0.1955754595314692 10-11 0.19959089771025545 12-13 0.1901428078778173 14-15 0.19581166177728018 16-17 0.19982709995606637 18-19 0.18943420114038445 20-21 0.18683597643646396 22-23 0.18234813376605585 24-25 0.18730838092808588 26-27 0.18471015622416537 28-29 0.20006330220187735 30-31 0.2088027852968826 32-33 0.21163721224661405 34-35 0.2047873471180964 36-37 0.1799861113079463 38-39 0.16534157206766723 40-41 0.1719552349503739 42-43 0.18683597643646396 44-45 0.19226862809011588 46-47 0.19628406626890207 48-49 0.19840988648120067 50-51 0.20573215610134019 52-53 0.231950605386356 54-55 0.18471015622416537 56-57 0.16652258329672198 58-59 0.16605017880510006 60-61 0.15683829121847292 62-63 0.1634519541011796 64-65 0.16061752715144814 66-67 0.161326133888881 68-69 0.17077422372131915 70-71 0.16463296533023436 72-73 0.16108993164307006 74-75 0.16817599901739866 76-77 0.1759706731291601 78-79 0.1790413023247025 80-81 0.16675878554253293 82-83 0.16179853838050293 84-85 0.15542107774360717 86-87 0.15494867325198527 88-89 0.16368815634699055 90-91 0.17077422372131915 92-93 0.156365886726851 94-95 0.1648691675760453 96-97 0.16156233613469198 98-99 0.1719552349503739 100-101 0.14195754973238287 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 211683.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.337202894568904 #Duplication Level Percentage of deduplicated Percentage of total 1 75.50172478965227 42.535559883645426 2 15.724261147817842 17.71721781303601 3 4.516893335148291 7.634074088261259 4 1.8332012951314054 4.1310973324161795 5 0.8096698211888562 2.280726649696296 6 0.431830589422812 1.4596876519436546 7 0.25031214543608343 0.9871320287085231 8 0.17145164381798716 0.7727284835505042 9 0.10247299894390409 0.5195737919445903 >10 0.5655638415964934 5.8395664671655325 >50 0.045323893881140116 1.8184801140421574 >100 0.035470873472196615 4.308621076066919 >500 0.005911812245366102 2.463496564749418 >1k 0.005911812245366102 7.532038054773514 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3755 1.7738788660402582 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3346 1.5806654289668987 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2234 1.0553516342833387 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1592 0.7520679506620749 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1541 0.7279753215893576 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1101 0.5201173452757188 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 905 0.42752606491782524 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 846 0.39965419991213275 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 801 0.378395997789147 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 778 0.36753069448184317 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 563 0.2659637287831333 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 545 0.25746044793393896 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 465 0.21966808860418643 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 414 0.1955754595314692 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 408 0.1927410325817378 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 329 0.15542107774360717 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 327 0.15447626876036338 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 317 0.1497522238441443 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 293 0.13841451604521857 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 285 0.1346352801122433 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 278 0.13132844867088997 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 274 0.12943883070440232 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 257 0.12140795434682992 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 251 0.11857352739709848 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 246 0.11621150493898895 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 241 0.11384948248087942 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 236 0.1114874600227699 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 212 0.10014975222384413 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0011810112290547659 0.0 22-23 0.0 0.0 0.0 0.0023620224581095317 0.0 24-25 0.0 0.0 0.0 0.005432651653651923 0.0 26-27 0.0 0.0 0.0 0.007558471865950501 0.0 28-29 0.0 0.0 0.0 0.012754921273791471 0.0 30-31 0.0 0.0 0.0 0.02456503356433913 0.0 32-33 0.0 0.0 0.0 0.035666539117453926 0.0 34-35 0.0 0.0 0.0 0.053145505307464466 0.0 36-37 0.0 0.0 0.0 0.06849865128517642 0.0 38-39 0.0 0.0 0.0 0.08786723544167457 0.0 40-41 0.0 0.0 0.0 0.10510999938587416 0.0 42-43 0.0 0.0 0.0 0.14313856096143762 0.0 44-45 0.0 0.0 0.0 0.1901428078778173 0.0 46-47 0.0 0.0 0.0 0.2487209648389337 0.0 48-49 0.0 0.0 0.0 0.3101335487497815 0.0 50-51 0.0 0.0 0.0 0.3772149865600922 0.0 52-53 0.0 0.0 0.0 0.47240449162190634 0.0 54-55 0.0 0.0 0.0 0.5536580641808743 0.0 56-57 0.0 0.0 0.0 0.7100239509077253 0.0 58-59 0.0 0.0 0.0 0.8796171633999896 0.0 60-61 0.0 0.0 0.0 1.0456673422050897 0.0 62-63 0.0 0.0 0.0 1.275492127379147 0.0 64-65 0.0 0.0 0.0 1.4363458567764062 0.0 66-67 0.0 0.0 0.0 1.7171903270456297 0.0 68-69 0.0 0.0 0.0 2.0556681452927252 0.0 70-71 0.0 0.0 0.0 2.3362764133161376 0.0 72-73 0.0 0.0 0.0 2.7394736469154353 0.0 74-75 0.0 0.0 0.0 3.1261367233079653 0.0 76-77 0.0 0.0 0.0 3.441466721465588 0.0 78-79 0.0 0.0 0.0 3.8073440002267542 0.0 80-81 0.0 0.0 0.0 4.3954875922960275 0.0 82-83 0.0 0.0 0.0 4.854900960398332 0.0 84-85 0.0 0.0 0.0 5.481073114043168 0.0 86-87 0.0 0.0 0.0 6.220858547923074 0.0 88-89 0.0 0.0 0.0 7.202515081513395 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTATAC 40 5.3547556E-4 47.541965 3 CATTCCG 50 2.8162292E-5 47.50817 9 CTATACC 75 3.0498876E-4 31.702158 4 GGTTATA 60 0.0039221365 31.702158 1 TATAAGG 75 3.0541344E-4 31.694641 2 TAGGAGT 65 0.005801555 29.26353 4 ATAGCAC 65 0.0058082836 29.256592 3 ATGGGGT 135 4.1566636E-7 28.179695 5 ACAATGC 85 6.3833036E-4 27.945984 8 TTTGCGT 70 0.008340315 27.166834 3 GGTATCA 2500 0.0 25.868961 1 CATGGGA 430 0.0 25.435453 4 GTACATG 870 0.0 25.143091 1 TACATGG 855 0.0 25.022085 2 GTAGGAC 115 1.2692776E-4 24.8045 3 ACATGGG 885 0.0 24.17388 3 TAGGCAT 120 1.6914745E-4 23.776619 5 TTTAGGC 100 0.0016407332 23.77098 3 CCGGTGT 50 0.0016479904 23.759716 12-13 GTATCAA 3335 0.0 23.527088 1 >>END_MODULE