FastQCFastQC Report
Thu 26 May 2016
SRR937835_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937835_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211683
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34191.6151509568552977No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22771.0756650274230808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21191.0010251177468197No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17640.8333215232210428No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14930.7052999059915062No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8620.4072126717780833No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6090.287694335397741No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC6020.2843875039563876No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.25509842547582945No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT5320.25131918954285415No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.2352574368277094No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4780.22580934699527122No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA4600.2173060661460769No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4130.19510305503984732No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3110.1469177968944129No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3050.14408336994468143No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.13699730257035284No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC2820.1332180666373776No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT2510.11857352739709848No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2400.11337707798925753No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.11101505553114799No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA2220.10487379714006322No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.10345658366519749No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT2140.10109456120708796No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC156.5881404E-495.0806353
ACGTATA200.002064769671.310482
CCCTAGA554.9179318E-543.228691
CGCAGAA500.00161360737.9963159
GTGTAGC905.620732E-736.9845471
GTACTGA550.002561223234.5829541
GTAGCCC902.3898305E-531.6935463
CAAGCGT300.00395003231.66359754-55
GGACTCT650.00580258629.26251
CTATACC700.00834177127.1658954
TAGTATC700.00834177127.1658954
TCGTTAG350.00838965627.14022492-93
TAGGACA1955.2386895E-1026.8176164
TGTAGCC1309.905298E-625.5986332
GAATAGG950.001223941924.9975766
CCCTGGC1551.4027592E-624.5137527
TATAGGT1401.759E-523.7701593
AACCTGA1000.001650138423.7476987
GTCTATA1050.002176572822.64361
GAGTACT18250.022.6416412-13
Produced by FastQC (version 0.11.5)