FastQCFastQC Report
Thu 26 May 2016
SRR937834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937834_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206629
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32121.5544768643317248No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22061.0676139360883514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21081.020185937114345No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17180.8314418595647272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14250.6896418218159116No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8720.42201239903401744No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC6280.30392636077220525No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5620.2719850553407315No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT5470.2647256677426692No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5330.25795023931781114No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.257466280144607No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA4450.21536183207584608No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4310.208586403650988No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG3850.18632428168359716No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3180.15389901707891923No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2870.13889628270959062No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC2790.13502460932395743No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.11227852818336244No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.11034269149054585No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.10647101810491266No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT2170.10501914058530022No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA2130.10308330389248363No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.10211538554607534No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG200.002071683371.249829
TGGCGGT205.384218E-447.49987880-81
TATTAGG459.6068427E-442.2221152
CGCAGAA853.6214806E-739.117559
TTTAGGC902.403506E-531.6665863
GTCCTAC1252.1395135E-730.3999231
CATACCA650.00583320129.2306944
GTATACA1354.1969724E-728.1480771
GCACTGC700.00837602927.142796
GGTAGAA908.9227577E-426.3888232
TCCTACA1401.7703673E-523.749942
CCTACAG1703.1283744E-622.3528843
TGTAGGA2405.857146E-921.7707772
ACTTACT1553.8865808E-521.4515578
TACATGG14400.021.4409182
GTACATG14650.021.3992631
GAGTACT16500.021.01509712-13
GTAGGAC2509.37689E-920.8999483
CATGGGC2056.773771E-720.8536054
CTACAGT1404.8333168E-420.3570924
Produced by FastQC (version 0.11.5)