##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937834_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 206629 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.123216005497774 33.0 31.0 34.0 31.0 34.0 2 32.330103712450814 34.0 31.0 34.0 31.0 34.0 3 32.38715765937985 34.0 31.0 34.0 30.0 34.0 4 36.0300877417981 37.0 35.0 37.0 35.0 37.0 5 35.959497456794544 37.0 35.0 37.0 35.0 37.0 6 36.02521427292394 37.0 35.0 37.0 35.0 37.0 7 35.961844658784585 37.0 35.0 37.0 35.0 37.0 8 35.959753955156344 37.0 35.0 37.0 35.0 37.0 9 37.58180119925083 39.0 37.0 39.0 35.0 39.0 10-11 37.54851206752198 39.0 37.0 39.0 35.0 39.0 12-13 37.466333380116055 39.0 37.0 39.0 35.0 39.0 14-15 38.577510901180375 40.0 38.0 41.0 34.0 41.0 16-17 38.552449075396 40.0 38.0 41.0 34.0 41.0 18-19 38.57557748428343 40.0 38.0 41.0 34.0 41.0 20-21 38.50804098166279 40.0 38.0 41.0 34.0 41.0 22-23 38.337818021671694 40.0 38.0 41.0 34.0 41.0 24-25 38.34076291324064 40.0 38.0 41.0 34.0 41.0 26-27 38.13762588987993 40.0 37.0 41.0 34.0 41.0 28-29 37.95132338635913 40.0 37.0 41.0 33.0 41.0 30-31 37.83884643491475 40.0 37.0 41.0 33.0 41.0 32-33 37.607409898900926 40.0 35.5 41.0 33.0 41.0 34-35 37.39260703967014 40.0 35.0 41.0 33.0 41.0 36-37 37.133584830783676 39.5 35.0 41.0 33.0 41.0 38-39 36.919299807868214 39.0 35.0 41.0 32.5 41.0 40-41 36.669794656122804 39.0 35.0 41.0 31.5 41.0 42-43 36.324823717871155 39.0 35.0 41.0 31.0 41.0 44-45 35.86631837738169 39.0 35.0 41.0 29.5 41.0 46-47 35.30256401569964 39.0 35.0 41.0 26.5 41.0 48-49 34.79165073634388 39.0 35.0 40.5 23.5 41.0 50-51 34.40430433288648 38.5 34.5 40.0 22.5 41.0 52-53 34.14482962217308 38.0 34.0 40.0 20.0 41.0 54-55 33.87870531241984 38.0 34.0 40.0 18.0 41.0 56-57 33.542053632355575 38.0 33.5 40.0 11.0 41.0 58-59 33.25091589273529 38.0 33.0 40.0 7.5 41.0 60-61 32.836692816593995 37.5 33.0 40.0 2.0 41.0 62-63 32.48418179442382 37.0 33.0 40.0 2.0 41.0 64-65 32.0772955393483 36.5 32.0 39.5 2.0 41.0 66-67 31.610981517599175 36.0 31.0 39.0 2.0 40.5 68-69 31.154748365427892 35.0 31.0 39.0 2.0 40.0 70-71 30.708087925702586 35.0 30.5 38.0 2.0 40.0 72-73 30.184117427853785 35.0 29.5 37.5 2.0 40.0 74-75 29.68983298568933 35.0 29.0 37.0 2.0 39.0 76-77 28.446321668304062 33.5 28.0 35.5 2.0 38.5 78-79 28.968537814149997 34.0 29.0 36.0 2.0 39.0 80-81 28.711579691137256 34.0 29.0 36.0 2.0 37.5 82-83 28.324939868072732 34.0 28.5 35.0 2.0 37.0 84-85 27.917826152185803 34.0 27.5 35.0 2.0 37.0 86-87 27.481265940405265 34.0 26.5 35.0 2.0 36.0 88-89 27.121142240440598 34.0 26.0 35.0 2.0 36.0 90-91 26.616041310755023 33.0 25.0 35.0 2.0 35.0 92-93 26.148519810868756 33.0 24.5 35.0 2.0 35.0 94-95 25.68885780795532 33.0 22.0 35.0 2.0 35.0 96-97 25.155740481732963 32.5 19.0 35.0 2.0 35.0 98-99 24.374782823321027 32.0 2.0 34.0 2.0 35.0 100-101 22.078065034433696 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.309233393240973 1101 2 0.1526051445705079 1101 3 0.4082170021985547 1101 4 0.15133757353204658 1101 5 0.28596953596199626 1101 6 0.04245301152531056 1101 7 0.23626668629825076 1101 8 0.045917270787519726 1101 9 0.27088736241990574 1101 10-11 -0.04192164029546319 1101 12-13 0.12375045731577217 1101 14-15 0.40554284914880157 1101 16-17 0.4248650279049997 1101 18-19 0.27236757593706074 1101 20-21 0.29583499825645276 1101 22-23 0.2884603775030783 1101 24-25 0.09351805372640598 1101 26-27 -0.04766199098902746 1101 28-29 -0.1386316925800486 1101 30-31 -0.0901397202257499 1101 32-33 0.3926714290964597 1101 34-35 0.4473585991011859 1101 36-37 0.49103674818911003 1101 38-39 0.1348235166984182 1101 40-41 0.01806331823309648 1101 42-43 0.013229557855552798 1101 44-45 -0.2919168832795478 1101 46-47 0.061482325383757086 1101 48-49 -0.011712917592390681 1101 50-51 0.06158125226658484 1101 52-53 0.26237037441217836 1101 54-55 0.35800760830262135 1101 56-57 0.3880739829523989 1101 58-59 0.44444904788769435 1101 60-61 0.3826614772254118 1101 62-63 0.3419643789860132 1101 64-65 0.5601707904935651 1101 66-67 0.16475395112068014 1101 68-69 0.7922264567199413 1101 70-71 0.948737493083037 1101 72-73 0.4421156593298896 1101 74-75 0.6283551563643677 1101 76-77 0.5186023813067671 1101 78-79 0.8755085239513463 1101 80-81 0.6047583853790499 1101 82-83 0.6740798018520557 1101 84-85 0.6260134581278614 1101 86-87 0.570084422256258 1101 88-89 0.8077428441370422 1101 90-91 0.7569613182279937 1101 92-93 1.102230463303485 1101 94-95 0.8688184952173685 1101 96-97 0.6819200428011385 1101 98-99 0.6554474886945592 1101 100-101 1.1414177926985722 1102 1 0.2719708923835711 1102 2 0.045413293277555056 1102 3 0.20041811338587934 1102 4 0.16848552606651168 1102 5 -0.06292270630427765 1102 6 0.2178251220953058 1102 7 0.25281446049401524 1102 8 0.2593749839365671 1102 9 0.36866498777143875 1102 10-11 0.2821918105585084 1102 12-13 0.17774935984680695 1102 14-15 0.392055984480443 1102 16-17 0.3668535044808934 1102 18-19 0.42555880990372685 1102 20-21 0.3765847067412551 1102 22-23 0.6120936856860837 1102 24-25 0.2207472875558807 1102 26-27 0.37723855088627545 1102 28-29 0.45829710912101973 1102 30-31 0.5761268275484426 1102 32-33 0.8092123441112093 1102 34-35 0.637735168138839 1102 36-37 0.6025841794258255 1102 38-39 0.7702408978631041 1102 40-41 0.7592149747253032 1102 42-43 0.7861735183466649 1102 44-45 0.9040327703318098 1102 46-47 0.7207353605713607 1102 48-49 0.4730682745332615 1102 50-51 0.764321495081596 1102 52-53 1.0078518889193404 1102 54-55 1.1921697593417804 1102 56-57 0.9228243087634951 1102 58-59 0.8882466334559709 1102 60-61 0.9184578281411149 1102 62-63 0.3262654284407063 1102 64-65 0.296323818821989 1102 66-67 0.16425666049718046 1102 68-69 0.35190990551615187 1102 70-71 0.2990873113147394 1102 72-73 0.6547502707572868 1102 74-75 1.023606888307576 1102 76-77 0.6815165043604736 1102 78-79 0.46504655811006756 1102 80-81 0.5635775773675284 1102 82-83 0.6759146327732424 1102 84-85 0.43274745908814793 1102 86-87 0.0305498176880441 1102 88-89 -0.14742383396159298 1102 90-91 0.09439872820124151 1102 92-93 0.3253510477057091 1102 94-95 0.5512212663264968 1102 96-97 0.2843790591945812 1102 98-99 0.4666056614087353 1102 100-101 0.7260000685748338 1103 1 0.40919069833874033 1103 2 0.5134795361681554 1103 3 0.45538633018079366 1103 4 0.23305561639772776 1103 5 0.23553831744948894 1103 6 0.11897935427998618 1103 7 0.4274715364391426 1103 8 0.340204692869321 1103 9 0.2995783367175733 1103 10-11 0.37277394505164807 1103 12-13 0.3935907783814301 1103 14-15 0.7902409961900432 1103 16-17 0.70410676644142 1103 18-19 0.5521814261633509 1103 20-21 0.5241758743558478 1103 22-23 0.881765816833628 1103 24-25 0.7084271135846549 1103 26-27 0.718096697423583 1103 28-29 0.6914938304324991 1103 30-31 1.0012188315631718 1103 32-33 0.9790517552855178 1103 34-35 0.9581433314041448 1103 36-37 0.9692452700915979 1103 38-39 1.3248243705964668 1103 40-41 1.067896808114405 1103 42-43 1.5716703400261665 1103 44-45 1.6071609068323127 1103 46-47 1.7330304425385705 1103 48-49 1.4843847683439009 1103 50-51 1.4757132649042788 1103 52-53 1.6745213435864557 1103 54-55 1.4732838443200365 1103 56-57 1.422665392738061 1103 58-59 1.8568732644362385 1103 60-61 1.7274825855850722 1103 62-63 2.147284163803704 1103 64-65 2.4272957157774897 1103 66-67 2.5629351482857388 1103 68-69 2.357839982464963 1103 70-71 2.1947882146268824 1103 72-73 2.6166438806602663 1103 74-75 3.028884573152002 1103 76-77 2.7463626067358504 1103 78-79 2.820474794978587 1103 80-81 2.974726791151401 1103 82-83 3.1166807752958228 1103 84-85 2.951775562132646 1103 86-87 2.7326324540212674 1103 88-89 2.769078340678753 1103 90-91 2.5647232514531666 1103 92-93 2.408882329070714 1103 94-95 2.8986451539143196 1103 96-97 2.9349812672909366 1103 98-99 2.4052724395954215 1103 100-101 2.3343389779090593 1104 1 0.299712240288855 1104 2 0.26386502369649634 1104 3 0.08540900591775369 1104 4 0.22371321276960998 1104 5 0.24669478003452383 1104 6 0.2317231184523223 1104 7 0.288242511495838 1104 8 0.15598700579449343 1104 9 0.1935465906858269 1104 10-11 0.33925920582262137 1104 12-13 0.18556356749707703 1104 14-15 0.20071718666623184 1104 16-17 0.18945824036432413 1104 18-19 0.18365534906583747 1104 20-21 0.47864299453725323 1104 22-23 0.46398803905584174 1104 24-25 0.39563799793839394 1104 26-27 0.24526223030339622 1104 28-29 0.29149383043250054 1104 30-31 0.2992233667105566 1104 32-33 0.25986808181612986 1104 34-35 0.18440183480550587 1104 36-37 0.16788472587391112 1104 38-39 0.16541393975746388 1104 40-41 0.11515304394206538 1104 42-43 0.17956149648874487 1104 44-45 0.5348706573992033 1104 46-47 0.1685859980941231 1104 48-49 -0.08327963074906108 1104 50-51 0.4197495460834162 1104 52-53 0.575201615695299 1104 54-55 0.16067613456947072 1104 56-57 0.16833432697843165 1104 58-59 0.49510455695324396 1104 60-61 0.35619006857827173 1104 62-63 0.17363337015290625 1104 64-65 0.04443857292034181 1104 66-67 -0.10173605352832382 1104 68-69 -0.10633235313594369 1104 70-71 0.4391419561234855 1104 72-73 0.9831291414312382 1104 74-75 0.35663967519282025 1104 76-77 0.3221449196610209 1104 78-79 0.15385347978584107 1104 80-81 0.2195793988611534 1104 82-83 0.1680639952504741 1104 84-85 -0.12981173945465585 1104 86-87 -0.010315391783723982 1104 88-89 0.07338673070142931 1104 90-91 -0.09831529729966704 1104 92-93 0.43940387102082923 1104 94-95 0.7189626142317813 1104 96-97 0.3619200428011382 1104 98-99 0.4066329838131111 1104 100-101 0.9868106332378552 1105 1 0.254459715036333 1105 2 0.28982461965609474 1105 3 0.22672213723088674 1105 4 0.08361220266859704 1105 5 0.023664477004217588 1105 6 -0.05393344720424409 1105 7 0.18925261250593906 1105 8 0.05356276337025179 1105 9 0.30607184321108605 1105 10-11 0.21117839774181135 1105 12-13 0.0952605371940507 1105 14-15 0.09511112606017491 1105 16-17 -0.15155186064577464 1105 18-19 0.4140088844193812 1105 20-21 0.6296025904968445 1105 22-23 0.3885839986518036 1105 24-25 0.2863955736959767 1105 26-27 -0.012515547474379218 1105 28-29 0.23467564861431356 1105 30-31 0.3691223566095445 1105 32-33 0.3175953545433998 1105 34-35 0.27182607722974694 1105 36-37 0.2640968470860301 1105 38-39 0.21591899026251582 1105 40-41 0.39080960959862665 1105 42-43 0.3715311934584449 1105 44-45 0.6931534856820321 1105 46-47 0.7739395334476598 1105 48-49 0.17060925813981953 1105 50-51 0.16439601072988097 1105 52-53 0.17595919145286842 1105 54-55 0.4923933062866439 1105 56-57 0.2975262461703494 1105 58-59 0.2017712236199074 1105 60-61 0.029523401911610847 1105 62-63 0.2599465014660396 1105 64-65 0.29807493655670925 1105 66-67 0.2723043505120799 1105 68-69 0.3229605761569836 1105 70-71 0.24580862279015037 1105 72-73 -0.18813348483138626 1105 74-75 0.162094220647365 1105 76-77 -0.5880065954904943 1105 78-79 -0.30276268183031974 1105 80-81 -0.6730468637651121 1105 82-83 -0.3591077219212444 1105 84-85 -0.2712763859193039 1105 86-87 -0.23874973521806808 1105 88-89 -0.2771183198036198 1105 90-91 -0.3306385296229024 1105 92-93 -0.15039410877715298 1105 94-95 0.008710088979253072 1105 96-97 0.17737458825568453 1105 98-99 0.7172390444191663 1105 100-101 1.3917601281873502 1106 1 0.08104074270431738 1106 2 0.09120801886557928 1106 3 0.25458774987910715 1106 4 0.1838098311270997 1106 5 0.11437593945480984 1106 6 0.14735113777599196 1106 7 0.2557545887905235 1106 8 0.2746101942002923 1106 9 0.29026156653124247 1106 10-11 0.0753582396390442 1106 12-13 -0.04979875134350209 1106 14-15 0.29792733159377605 1106 16-17 0.19770703263485245 1106 18-19 0.02962153264072498 1106 20-21 0.2565294679671979 1106 22-23 -0.06279940055767952 1106 24-25 0.0392611863441914 1106 26-27 0.08313662643866593 1106 28-29 0.1539938304324977 1106 30-31 0.25084172419848016 1106 32-33 0.023128951381345075 1106 34-35 -0.4138952666437703 1106 36-37 -0.12523121615507904 1106 38-39 0.10514823927437078 1106 40-41 -0.3936271492946517 1106 42-43 -0.4084095180040066 1106 44-45 -0.29424770008871803 1106 46-47 -0.10392607920055497 1106 48-49 -0.09678204620800557 1106 50-51 0.139544232073753 1106 52-53 0.2712161084489182 1106 54-55 0.46457710075303993 1106 56-57 0.08497683905572018 1106 58-59 0.11753209318512603 1106 60-61 0.3605016627811679 1106 62-63 0.29650776628817965 1106 64-65 0.08280813813773591 1106 66-67 0.5415731252156348 1106 68-69 0.7042352797143003 1106 70-71 0.15613470974667365 1106 72-73 0.06324991437810112 1106 74-75 0.28403097954064904 1106 76-77 0.18294202111029634 1106 78-79 0.1726819822013006 1106 80-81 0.16470017180801122 1106 82-83 -0.12170653615049432 1106 84-85 0.013110965859350898 1106 86-87 0.1766652845447787 1106 88-89 -0.3380866992502618 1106 90-91 0.07390692492255369 1106 92-93 0.18453672126237564 1106 94-95 0.7515954644733256 1106 96-97 0.7688765645402711 1106 98-99 0.2769228388855751 1106 100-101 0.3532719858948603 1107 1 0.3048360902534668 1107 2 0.26304407535962326 1107 3 -0.058483421541552616 1107 4 0.16650160625864174 1107 5 0.3275440369347322 1107 6 0.0961534086150948 1107 7 0.25398914985393617 1107 8 0.38112501004077615 1107 9 0.3723717853072017 1107 10-11 0.3251332327157499 1107 12-13 0.18310072248646492 1107 14-15 0.3773201590653841 1107 16-17 0.14039950009539126 1107 18-19 0.2188004304529656 1107 20-21 -0.03682763532828659 1107 22-23 0.13399511619667948 1107 24-25 0.05580784931844107 1107 26-27 0.3938538792772093 1107 28-29 0.39404160113313935 1107 30-31 -0.6266506601825768 1107 32-33 -0.48459051690998933 1107 34-35 -0.6265960420522418 1107 36-37 -0.5718180342110131 1107 38-39 -0.38547070284693064 1107 40-41 -0.9831625965391808 1107 42-43 -0.5250669536174115 1107 44-45 -0.6735228316312245 1107 46-47 -0.6491351625569663 1107 48-49 -0.3690404233888387 1107 50-51 -1.3427840703355471 1107 52-53 -1.295074392797268 1107 54-55 -0.7087647713045513 1107 56-57 -0.4250273149182462 1107 58-59 -0.9256173114119406 1107 60-61 -0.7176103560501801 1107 62-63 -0.5305138343764568 1107 64-65 -0.6983639748503556 1107 66-67 -0.6658832580576934 1107 68-69 -0.7525460827891628 1107 70-71 -0.6427264090569835 1107 72-73 -0.6876847297647934 1107 74-75 -0.6897297515587688 1107 76-77 -0.9025259932901477 1107 78-79 -0.2289207594215199 1107 80-81 -0.29403702010558774 1107 82-83 -0.5784186657544801 1107 84-85 -0.758870479723587 1107 86-87 -0.11525523608662169 1107 88-89 -0.20948658847762403 1107 90-91 -0.3321440304914596 1107 92-93 -0.28273342551137404 1107 94-95 -0.1706906058672999 1107 96-97 0.29656972432979956 1107 98-99 0.7234058075923002 1107 100-101 0.6830455943135796 1108 1 0.1330593762446881 1108 2 0.04462416793390389 1108 3 -0.13361100788487335 1108 4 0.08753653920162918 1108 5 0.13456227485853844 1108 6 0.1683138706952505 1108 7 0.2984564521445563 1108 8 0.360790318423895 1108 9 0.3244230572144673 1108 10-11 0.2698458172787994 1108 12-13 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-0.8444691549876282 2316 72-73 -1.0736764141243142 2316 74-75 -1.2597492136960646 2316 76-77 -1.4659106358945344 2316 78-79 -1.61822985354749 2316 80-81 -0.9479206011388506 2316 82-83 -0.9127693380828603 2316 84-85 -1.1553672950102118 2316 86-87 -1.302954280672612 2316 88-89 -1.0175854915207907 2316 90-91 -0.564981963966332 2316 92-93 -0.3518461289791759 2316 94-95 -0.305620719101551 2316 96-97 -0.2347466238655258 2316 98-99 -0.7683670161868932 2316 100-101 -0.12680047787325677 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 5.0 5 8.0 6 22.0 7 48.0 8 96.0 9 192.0 10 325.0 11 543.0 12 830.0 13 1157.0 14 1482.0 15 2115.0 16 2891.0 17 3924.0 18 3543.0 19 2253.0 20 1945.0 21 1837.0 22 1847.0 23 1932.0 24 2125.0 25 2353.0 26 2475.0 27 2875.0 28 3413.0 29 4074.0 30 4938.0 31 5950.0 32 7481.0 33 9496.0 34 12997.0 35 18565.0 36 25148.0 37 38599.0 38 35461.0 39 3683.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.319272734313735 23.295350030496365 15.513452285291072 18.87192494989883 2 15.2471337517967 25.574338548800025 37.992730933218475 21.185796766184804 3 19.99186948589017 28.367750896534368 31.593338785940016 20.047040831635442 4 12.276592346669634 21.45584598483272 37.44585706749778 28.82170460099986 5 11.377300583798855 41.92702030225871 33.80950536843226 12.88617374551017 6 27.1234918622265 40.41494659510524 17.087146528318872 15.37441501434939 7 24.331531392011772 38.50282390177565 20.968983056589348 16.196661649623238 8 24.921477624147627 36.159977544294364 19.996225118449008 18.922319713109 9 24.849851666513413 19.380145090960127 22.155650949285917 33.61435229324054 10-11 23.286663537064015 30.189373224474785 26.87255903092015 19.651404207541052 12-13 25.69802389906059 28.975070056480767 27.16594311268567 18.16096293177297 14-15 21.598855920514545 31.410160238882245 27.95033610964579 19.040647730957417 16-17 18.243566972690182 33.77962435088976 29.06682992222776 18.9099787541923 18-19 17.83946106306472 32.07487816327815 32.85453639131003 17.231124382347105 20-21 19.910564344791872 30.231235692956943 32.58884280522095 17.26935715703023 22-23 18.992919608763618 31.48281686323665 32.16883563135505 17.355427896644677 24-25 19.32448978604165 31.30054348615151 32.6060233558697 16.76894337193714 26-27 18.66489859820886 31.384102386650437 33.32042772415228 16.630571290988417 28-29 17.737646127898056 32.68086465610489 32.68110663979035 16.900382576206713 30-31 18.59104965905076 31.726427558571157 32.51600694965372 17.166515832724354 32-33 18.083860445532814 32.243295955553194 32.541656785833545 17.131186813080447 34-35 18.76237120636503 32.234826670022116 32.39695299304551 16.605849130567346 36-37 18.419486132149892 31.734412884929025 32.50608578660304 17.34001519631804 38-39 18.21767515692376 32.678133272677115 32.05527781676338 17.048913753635745 40-41 18.531567523357136 32.567869388782285 31.65124849669818 17.249314591162403 42-43 17.832543550971213 33.3013064081279 31.09169058602013 17.77445945488076 44-45 18.57459504716182 32.65417729360351 30.87272357703904 17.898504082195625 46-47 19.342638255036807 32.81170600448146 30.272130243092693 17.57352549738904 48-49 19.234957338998882 32.943100920006394 29.78865502906175 18.03328671193298 50-51 18.477319253347787 33.45198399063055 29.96868784149369 18.10200891452797 52-53 19.09513934428213 34.16421258442083 28.6676329741541 18.07301509714294 54-55 18.82988351102701 33.15265524200379 29.022789637466182 18.994671609503023 56-57 19.280691480866675 33.33099419732951 28.53350691335679 18.854807408447023 58-59 20.02768246470728 33.37213072705187 28.18675016575602 18.41343664248484 60-61 19.0154818539508 33.27558087199764 29.38914673158172 18.31979054246984 62-63 18.86860024488334 33.119504038639306 29.24710471424631 18.76479100223105 64-65 18.983056589346123 33.826326411103956 28.702166685218433 18.488450314331484 66-67 19.00241495627429 33.22645901591741 28.301206510218798 19.469919517589496 68-69 18.74620393604948 32.9274519245893 28.8955782963144 19.43076584304682 70-71 19.607605902366075 33.01158114301477 28.280396265771017 19.100416688848128 72-73 19.08420405654579 32.406874156096194 29.259445673163007 19.24947611419501 74-75 18.58572610814552 33.85875167570864 28.429697670704503 19.125824545441347 76-77 18.708167778966168 32.35484854497675 28.891636701527858 20.04534697452923 78-79 18.51264827299169 32.795977331352326 28.4650266903484 20.226347705307578 80-81 18.904897182873654 32.669663987146045 28.43211746657052 19.993321363409784 82-83 18.401165393429174 32.684252427701324 28.778877728063573 20.135704450805928 84-85 18.162789741008623 32.809123620410546 28.423233000287958 20.604853638292877 86-87 18.103944751220787 32.871474962372176 27.915733028761693 21.108847257645344 88-89 18.44150627453068 32.364043769267624 27.939205048662096 21.2552449075396 90-91 18.45180619384004 31.66399252692895 28.491325381095162 21.392875898135845 92-93 18.253248057457164 32.332237136627725 28.50861591251721 20.905898893397897 94-95 17.931897265146713 32.445106930779325 28.30023859187239 21.32275721220158 96-97 17.355743869447174 32.34323352481985 28.847112457593077 21.453910148139904 98-99 18.095781982378746 32.71450038838382 27.633568944781743 21.556148684455696 100-101 17.536267661879386 32.86428609461298 27.918233787858988 21.681212455648655 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2160.0 1 1310.5 2 444.0 3 428.5 4 406.0 5 457.5 6 675.5 7 984.0 8 1166.0 9 1133.0 10 995.0 11 899.5 12 878.5 13 866.0 14 840.0 15 774.5 16 713.5 17 698.0 18 736.0 19 836.5 20 950.0 21 1026.5 22 1133.0 23 1350.0 24 1585.0 25 1871.0 26 2281.0 27 2828.5 28 3294.5 29 3796.0 30 4402.5 31 4958.0 32 5654.5 33 6061.5 34 6584.0 35 7265.0 36 7687.5 37 7841.5 38 7452.0 39 7072.5 40 6937.0 41 6679.0 42 6336.0 43 6333.0 44 6170.5 45 5881.0 46 5943.5 47 6853.0 48 9108.0 49 8683.0 50 6059.5 51 4645.5 52 3851.0 53 3430.0 54 2987.0 55 2554.5 56 2161.0 57 1858.0 58 1697.0 59 1564.0 60 1373.0 61 1131.5 62 867.5 63 630.5 64 408.0 65 277.0 66 203.0 67 152.0 68 112.5 69 84.5 70 68.5 71 41.5 72 27.0 73 21.5 74 16.0 75 12.5 76 10.5 77 12.5 78 12.0 79 8.0 80 4.5 81 2.5 82 3.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02274608114059498 2 0.0 3 0.0 4 0.0 5 0.02468191783341157 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0048395917320414854 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.679183464082969E-4 24-25 0.0 26-27 2.4197958660207423E-4 28-29 0.0016938571062145196 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.4197958660207423E-4 42-43 0.01548669354253275 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.4197958660207423E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.4197958660207423E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0016938571062145196 84-85 2.4197958660207423E-4 86-87 0.0 88-89 0.0 90-91 0.009921163050685045 92-93 0.0026617754526228167 94-95 0.0 96-97 0.0 98-99 0.0016938571062145196 100-101 0.019358366928165942 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 206629.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.196087246351055 #Duplication Level Percentage of deduplicated Percentage of total 1 74.80093396104725 42.035198109835555 2 15.481035760868203 17.399472725632602 3 4.779429438235557 8.057557010965917 4 1.9435877384821494 4.36888104490724 5 0.9898183744262088 2.78119598636483 6 0.5290931436299069 1.7839778676523435 7 0.36088886901644235 1.4196379658639464 8 0.2293735173875147 1.0311915356088959 9 0.1463222725091475 0.7400465272807567 >10 0.6386737297475835 6.590380407258733 >50 0.05833086749364634 2.326800640822523 >100 0.03163708067452005 3.4968680117805544 >500 0.005931952626472509 2.041228642402567 >1k 0.004943293855393757 5.927563523623568 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3212 1.5544768643317248 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2206 1.0676139360883514 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2108 1.020185937114345 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1718 0.8314418595647272 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1425 0.6896418218159116 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 872 0.42201239903401744 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 628 0.30392636077220525 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 562 0.2719850553407315 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 547 0.2647256677426692 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 533 0.25795023931781114 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 532 0.257466280144607 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 445 0.21536183207584608 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 431 0.208586403650988 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 385 0.18632428168359716 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 318 0.15389901707891923 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 287 0.13889628270959062 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 279 0.13502460932395743 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 0.11227852818336244 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 228 0.11034269149054585 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 220 0.10647101810491266 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 217 0.10501914058530022 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 213 0.10308330389248363 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 211 0.10211538554607534 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.839591732041485E-4 0.0 22-23 0.0 0.0 0.0 0.0036296937990311137 0.0 24-25 0.0 0.0 0.0 0.006775428424858079 0.0 26-27 0.0 0.0 0.0 0.009679183464082971 0.0 28-29 0.0 0.0 0.0 0.017422530235349346 0.0 30-31 0.0 0.0 0.0 0.036054958403709064 0.0 32-33 0.0 0.0 0.0 0.0573491620246916 0.0 34-35 0.0 0.0 0.0 0.07888534523227621 0.0 36-37 0.0 0.0 0.0 0.0996955896800546 0.0 38-39 0.0 0.0 0.0 0.12268365040725165 0.0 40-41 0.0 0.0 0.0 0.14446181320143833 0.0 42-43 0.0 0.0 0.0 0.18438844499078058 0.0 44-45 0.0 0.0 0.0 0.2385918723896452 0.0 46-47 0.0 0.0 0.0 0.2988447894535617 0.0 48-49 0.0 0.0 0.0 0.3663570941155404 0.0 50-51 0.0 0.0 0.0 0.42540011324644655 0.0 52-53 0.0 0.0 0.0 0.5217079887140721 0.0 54-55 0.0 0.0 0.0 0.6018032318793587 0.0 56-57 0.0 0.0 0.0 0.7903053298423744 0.0 58-59 0.0 0.0 0.0 0.994294121347923 0.0 60-61 0.0 0.0 0.0 1.1716651583272435 0.0 62-63 0.0 0.0 0.0 1.4032396227054287 0.0 64-65 0.0 0.0 0.0 1.57020553746086 0.0 66-67 0.0 0.0 0.0 1.8714701227804422 0.0 68-69 0.0 0.0 0.0 2.2312937680577267 0.0 70-71 0.0 0.0 0.0 2.5190074965275926 0.0 72-73 0.0 0.0 0.0 2.935454365069763 0.0 74-75 0.0 0.0 0.0 3.3637582333554343 0.0 76-77 0.0 0.0 0.0 3.682445348910366 0.0 78-79 0.0 0.0 0.0 4.066466952847858 0.0 80-81 0.0 0.0 0.0 4.645040144413417 0.0 82-83 0.0 0.0 0.0 5.103591461024348 0.0 84-85 0.0 0.0 0.0 5.718703570166821 0.0 86-87 0.0 0.0 0.0 6.498603777785306 0.0 88-89 0.0 0.0 0.0 7.502819062183914 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 20 0.0020716833 71.24982 9 TGGCGGT 20 5.384218E-4 47.499878 80-81 TATTAGG 45 9.6068427E-4 42.222115 2 CGCAGAA 85 3.6214806E-7 39.11755 9 TTTAGGC 90 2.403506E-5 31.666586 3 GTCCTAC 125 2.1395135E-7 30.399923 1 CATACCA 65 0.005833201 29.230694 4 GTATACA 135 4.1969724E-7 28.148077 1 GCACTGC 70 0.008376029 27.14279 6 GGTAGAA 90 8.9227577E-4 26.388823 2 TCCTACA 140 1.7703673E-5 23.74994 2 CCTACAG 170 3.1283744E-6 22.352884 3 TGTAGGA 240 5.857146E-9 21.770777 2 ACTTACT 155 3.8865808E-5 21.451557 8 TACATGG 1440 0.0 21.440918 2 GTACATG 1465 0.0 21.399263 1 GAGTACT 1650 0.0 21.015097 12-13 GTAGGAC 250 9.37689E-9 20.899948 3 CATGGGC 205 6.773771E-7 20.853605 4 CTACAGT 140 4.8333168E-4 20.357092 4 >>END_MODULE